| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,851,227 | T→A | 14.7% | intergenic (+34/+34) | ilvD → / ← PP_5129 | dihydroxy‑acid dehydratase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,851,227 | 0 | T | A | 14.7% | 81.5 / 7.3 | 34 | intergenic (+34/+34) | ilvD/PP_5129 | dihydroxy‑acid dehydratase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (14/15); new base A (3/2); total (17/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01 | |||||||||||
AGGTCCGTCCACGCAAGGTAACCACCGCGCTCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGG‑TTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTCATCGAACAGTTTGCTTTTTCGAGCAGCGGCACCTACCCCTCCT > NC_002947/5851087‑5851375 | aGGTCCGTCCACGCAAGGTAACCACCGCGCTCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCgg < 1:504775/150‑1 (MQ=255) cACGCAAGGTAACCACCGCGCTCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCgg > 2:43518/1‑141 (MQ=255) cACGCAAGGTAACCACCGCGCTCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCgg < 1:43518/141‑1 (MQ=255) gCAAGGTAACCACCGCGCTCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGG‑TTTTTTTTGAtt < 2:587552/150‑1 (MQ=255) gCAAGGTAACCACCGCGCTCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGG‑TTTTTTTTGAtt < 1:62249/150‑1 (MQ=255) caccGCGCTCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCAGAGCGTAACAAGGCAATACTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGG‑TTTTTTTTGATTGTTCATTGAc > 2:30490/1‑150 (MQ=255) cgcgCTCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGG‑TTTTTTTTGATTGTTCATTGACGGt < 1:296404/150‑1 (MQ=255) cTCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGG‑TTTTTTTTGATTGTTCATTGACGGTCAGt > 2:353978/1‑150 (MQ=255) tCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGG‑TTTTTTTTGATTGTTCATTGACGGTCAGTg > 1:75021/1‑150 (MQ=255) aaGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACAGGCCCCCTGGGCGCCGG‑TTTTTTTTGATTGTTCATTGACGGTCAGGGaa > 1:67332/1‑150 (MQ=255) aGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGG‑TTTTTTTTGATTGTTCATTGACGGTCAGTGAAg > 2:144636/1‑150 (MQ=255) cTATGCCCTGCTGGCCACCAGTGCCGATAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGG‑TTTTCTTTGATTGTTCATTGCCGGTCAGTGAAGCGAg < 2:885/150‑1 (MQ=255) gctgGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGG‑TTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTggg < 1:76477/150‑1 (MQ=255) tgGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCGCTGGGCGCCGG‑TTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTc > 2:164283/1‑150 (MQ=255) accaGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGAt < 2:477564/150‑1 (MQ=255) accaGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGACTGTGATGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGG‑TTTTTGTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATc > 1:322841/1‑150 (MQ=255) cAAGGGTGCGGTGTGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGG‑TTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGAc > 2:3621/1‑150 (MQ=255) cAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGG‑TTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGa < 1:302792/137‑1 (MQ=255) cAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGG‑TTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGa > 2:302792/1‑137 (MQ=255) gtgcgTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGG‑TTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGga < 1:144636/150‑1 (MQ=255) tgcgTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGG‑TTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGgag > 1:80538/1‑150 (MQ=255) aaCAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATACGAACCGGCGCCCTGGGCGCCGG‑TTTTTTTTGATTGTTCATTGACGGTCa < 1:143216/97‑1 (MQ=255) aaCAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGG‑TTTTTTTTGATTGTTCATTGACGGTCa > 2:143216/1‑97 (MQ=255) aaTGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGG‑TTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGt < 2:75021/150‑1 (MQ=255) cTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGG‑TTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCt > 1:459895/1‑150 (MQ=255) ggAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGG‑TTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCg < 2:80538/150‑1 (MQ=255) ctctGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGG‑TTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTCATCGa > 2:258933/1‑150 (MQ=255) gTGAAATAAGAACCGGCGCCCTGGGCGCCGG‑TTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTCATCGAACAGTTTGCTTTTTCGag < 1:258933/150‑1 (MQ=255) aaaaaaCCGGCGCCCAGGGCGCCGG‑TTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTCATCGAACAGTTTGCTTTTTCGAGCAGCgg > 1:185211/5‑150 (MQ=255) aaCCGGCGCCCAGGGCGCCGG‑TTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGga > 2:208906/1‑95 (MQ=255) aaCCGGCGCCCAGGGCGCCGG‑TTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGga < 1:208906/95‑1 (MQ=255) aaCCGGCGCCCAGGGCGCCGG‑TTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTc > 2:21115/1‑114 (MQ=255) aaCCGGCGCCCAGGGCGCCGG‑TTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTc < 1:21115/114‑1 (MQ=255) cTGGGCGCCGG‑TTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTCATCGAACAGTTTGCTTTTTCGAGCAGCGGCACCTACCcctcct < 1:164283/150‑1 (MQ=255) | AGGTCCGTCCACGCAAGGTAACCACCGCGCTCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGG‑TTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTCATCGAACAGTTTGCTTTTTCGAGCAGCGGCACCTACCCCTCCT > NC_002947/5851087‑5851375 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |