| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,872,077 | T→A | 22.3% | D211V (GAC→GTC) | ptsP ← | phosphoenolpyruvate‑dependent regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,872,077 | 0 | T | A | 22.3% | 40.6 / 5.7 | 22 | D211V (GAC→GTC) | ptsP | phosphoenolpyruvate‑dependent regulator |
| Reads supporting (aligned to +/- strand): ref base T (11/6); new base A (2/3); total (13/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCTTCAAGCATCATCAGATAGACGTCGAACAGCGCACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATACCTTTGCCCTGACGGCCCAGGCCACG > NC_002947/5871936‑5872219 | gcTTCAAGCATCATCAGATAGACGTCGAACAGCGCACGCTCTTCCGGGGGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGACATCGACg < 2:514907/149‑1 (MQ=255) cTTCAAGCATCATCAGATAGACGTCGAACAGCGCACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGACATCGACgc < 2:286882/150‑2 (MQ=255) ttCAAGCATCATCAGATAGACGTCGAACAGCGCACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGt > 1:146980/1‑150 (MQ=255) tCAAGCATCATCAGATAGACGTCGAACAGCGCACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTc < 2:252558/150‑1 (MQ=255) aaGCATCATCAGATAGACGTCGAACAGCGCACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCtt > 1:474689/1‑150 (MQ=255) gcACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCAAACCTTCCAAGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTGCAGGTcggccggc > 1:180743/1‑150 (MQ=255) cGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATcac > 1:604068/1‑150 (MQ=255) gggTGGCCAGTTTGGCCGACAGCTTGCGCCTGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGcc > 1:383571/1‑150 (MQ=255) gggTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGcc > 1:244416/1‑150 (MQ=255) ggCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACTACCGCACGCCCCAc < 2:507735/150‑1 (MQ=255) gTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCATCGCgg > 1:278408/1‑150 (MQ=255) gtcgCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATAGCCGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCAc > 1:263731/1‑150 (MQ=255) acacCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAgcgc < 2:604068/150‑1 (MQ=255) gAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATACCTTTGcc > 2:62186/1‑150 (MQ=255) gAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATACCTTTGcc < 1:84834/150‑1 (MQ=255) aCAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATACCTTTGCCCt > 1:510419/1‑150 (MQ=255) gCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATACCTTTGCCCTGAc < 1:356595/150‑1 (MQ=255) gTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATACCTTTGCCCTGACGGCCCa > 2:334611/1‑150 (MQ=255) accGTCGATGACATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGCCACCAGGCGAACCCGGCACACCCACGAGGCGCGCTTCCTGAATACCTTTGCCCTGACGGCCCAGGcc > 2:10948/3‑150 (MQ=255) cGTCGATGACATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGaa > 2:77035/1‑109 (MQ=255) cGTCGATGACATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGaa < 1:77035/109‑1 (MQ=255) gTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATACCTTTGCCCTGACGGCCCAGGCCACg < 1:84258/150‑1 (MQ=255) | TCTTCAAGCATCATCAGATAGACGTCGAACAGCGCACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATACCTTTGCCCTGACGGCCCAGGCCACG > NC_002947/5871936‑5872219 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |