Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,951,645 T→C 16.7% Q456Q (CAA→CAG)  ppk ← polyphosphate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,951,6450TC16.7% 78.3 / 9.1 36Q456Q (CAA→CAG) ppkpolyphosphate kinase
Reads supporting (aligned to +/- strand):  ref base T (16/14);  new base C (2/4);  total (18/18)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

AGGCGGGCGTTGCCGGCGTGGTAGTTGCCGGTGCCCAGATGCGCGTAACGCACGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTCGACGATTTCCGAGTTAGCCCCCGAGCGGTACAGGGTCT  >  NC_002947/5951502‑5951790
                                                                                                                                               |                                                                                                                                                 
aGGCGGGCGTTGCCGGCGTGGTAGTTGCCGGTGCCCAGATGCGCGTAACGCACGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAgg                                                                                                                                             <  2:109375/150‑1 (MQ=255)
  gcgggcgTTGCCGGCTTGGTAGTTGCCGGTGCCCAGATGCGCGTAACGCACGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAggcg                                                                                                                                           >  2:139794/1‑150 (MQ=255)
     ggcgTTGCCGGGGTGGTAGTTTTCTTTTTTTACATGCGCGTAACGCACGATCTCGCCCTGCTCGCCGGGATGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCCTGCAAGCGGCt                                                                                                                                        <  1:288779/150‑1 (MQ=255)
        gTTGCCGGCGTGGTAGTTGCCGGTGCCCACATGCCCGTAACGCACGAGCTCTCGCTGCTCGCGGCGCAGACTAAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCCTGCAAGCGGCTggt                                                                                                                                     <  1:138028/150‑2 (MQ=255)
                 gTGGTAGTTGCCGGTGCCCAGATGCGCGTAACGCACGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGTCCATCTGCAg                                                                                                                            >  2:465455/1‑150 (MQ=255)
                  tGGTAGTTGCCGGTGCCCAGATGCGCGTAACGCACGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAg                                                                                                                                              >  1:332938/1‑131 (MQ=255)
                  tGGTAGTTGCCGGTGCCCAGATGCGCGTAACGCACGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAg                                                                                                                                              <  2:332938/131‑1 (MQ=255)
                     tAGTTGCCGGTGCCCAGATGCGCGTAACGCACGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTg                                                                                                                        <  1:139794/150‑1 (MQ=255)
                                          gcgTAACGCACGATCTCGCCCTGCTCGCGGCGGAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTGCCGCACCAGCCGCTTGCCGGCGGCTGGCCATCTGCAGGTTGAACTCTTCGTCAAAACGcgca                                                                                                   <  2:551373/150‑1 (MQ=255)
                                          gcgTAACGCACGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCa                                                                                                                                               <  2:479338/106‑1 (MQ=255)
                                          gcgTAACGCACGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCa                                                                                                                                               >  1:479338/1‑106 (MQ=255)
                                                 gCACGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACt                                                                                            >  1:427859/1‑150 (MQ=255)
                                                  cACGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTc                                                                                           <  2:501844/150‑1 (MQ=255)
                                                   aCGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCg                                                                                          >  2:381542/1‑150 (MQ=255)
                                                           gCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGa                                                                                                                      <  1:328939/114‑1 (MQ=255)
                                                           gCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGa                                                                                                                      >  2:328939/1‑114 (MQ=255)
                                                                         gcAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGcgca                                                                                                   >  2:102729/1‑119 (MQ=255)
                                                                         gcAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGcgca                                                                                                   <  1:102729/119‑1 (MQ=255)
                                                                         gcAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGc                                                                    >  1:469700/1‑150 (MQ=255)
                                                                                    atcatcTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGAACTCTGCAGGTTGGATGGTGCGCCAAAACGCTCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGcgcc                                                         <  1:381542/150‑1 (MQ=255)
                                                                                               gTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAg                                              >  1:308883/1‑150 (MQ=255)
                                                                                                      ttGAAGCCAACCACACCGTAGATCACTACCTCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTcg                                       >  1:263595/1‑150 (MQ=255)
                                                                                                      ttGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTcg                                       <  2:308883/150‑1 (MQ=255)
                                                                                                          aGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTc                                                                                                                >  1:85935/1‑73 (MQ=255)
                                                                                                          aGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTc                                                                                                                <  2:85935/73‑1 (MQ=255)
                                                                                                            ccAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTCGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTCGACGAtt                                 >  1:522018/1‑150 (MQ=255)
                                                                                                                ccACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTCGACGATTTCCg                             >  1:508929/1‑150 (MQ=255)
                                                                                                                              aCTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTCGACGATTTCCGAGTTAGCCCCCGAg               >  2:134672/1‑150 (MQ=255)
                                                                                                                                     cACCAACCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTCGACGATTTCCGAGTTAGCCCCCGAGCGGTACa        <  2:414480/150‑1 (MQ=255)
                                                                                                                                       ccAGCCGCCTGCAAGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATccac                                                 >  2:248422/1‑107 (MQ=255)
                                                                                                                                       ccAGCCGCCTGCAAGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATccac                                                 <  1:248422/107‑1 (MQ=255)
                                                                                                                                        cAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTCGACGATTTCCGAGTTAGCCCCCGAGCGGTACAggg     >  2:419751/1‑150 (MQ=255)
                                                                                                                                        cAGCCGCCTGCAAGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTAcgc                                                            >  1:404889/1‑95 (MQ=255)
                                                                                                                                        cAGCCGCCTGCAAGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTAcgc                                                            <  2:404889/95‑1 (MQ=255)
                                                                                                                                         aGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTCGACGATTTCCGAGTTAGCCCCCGAGCGGTACAGGGt    <  1:465455/150‑1 (MQ=255)
                                                                                                                                           ccGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTCGACGATTTCCGAGTGAGCCCCCGAGCGGTACAGGGTCt  <  2:263595/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
AGGCGGGCGTTGCCGGCGTGGTAGTTGCCGGTGCCCAGATGCGCGTAACGCACGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTCGACGATTTCCGAGTTAGCCCCCGAGCGGTACAGGGTCT  >  NC_002947/5951502‑5951790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑