| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 354,876 | A→C | 17.4% | F100C (TTC→TGC) | cbcW ← | choline/betaine/carnitine ABC transporter permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 354,876 | 0 | A | C | 17.4% | 41.7 / 6.4 | 23 | F100C (TTC→TGC) | cbcW | choline/betaine/carnitine ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base A (11/8); new base C (2/2); total (13/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.17e-01 | |||||||||||
GTCAGGGTAGGGATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGATAA > NC_002947/354735‑355014 | gtcagggtAGGGATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGAcc > 1:396120/1‑150 (MQ=255) tAGGGATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGCAGGTCACCTTCGCCa < 1:148185/150‑1 (MQ=255) gggATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAgg < 2:491109/150‑1 (MQ=255) gTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCCTGGCGCAGGTCACCTTCGCCACGGtggtatg > 2:419676/1‑144 (MQ=255) cACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCa > 1:103557/1‑125 (MQ=255) cACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCa < 2:103557/125‑1 (MQ=255) cACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCCTGGCGCAGGTCACCTTCGCCacgg > 1:287897/1‑125 (MQ=255) cACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCCTGGCGCAGGTCACCTTCGCCAcgg < 2:287897/128‑1 (MQ=255) cACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATcagcag > 2:499089/1‑150 (MQ=255) ggCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACg > 2:546378/1‑150 (MQ=255) aGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATg > 2:393746/1‑150 (MQ=255) ggATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGa > 2:250460/1‑150 (MQ=255) aTACCACCGTGGCGAATGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATc < 2:542497/71‑1 (MQ=255) aTACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATc > 1:542497/1‑71 (MQ=255) tACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATcagca < 2:280242/74‑1 (MQ=255) tACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATcagca > 1:280242/1‑74 (MQ=255) accaccGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTgg < 1:453623/117‑1 (MQ=255) accaccGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTgg > 2:453623/1‑117 (MQ=255) ccaccGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGATaa < 1:546378/150‑1 (MQ=255) accGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCa > 2:335714/1‑97 (MQ=255) accGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCa < 1:335714/97‑1 (MQ=255) gAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGc > 2:323048/1‑75 (MQ=255) gAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGc < 1:323048/75‑1 (MQ=255) | GTCAGGGTAGGGATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGATAA > NC_002947/354735‑355014 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |