| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,071,939 | C→G | 27.6% | L214F (TTG→TTC) | pstB‑II ← | phosphate ABC transporter ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,071,939 | 0 | C | G | 27.6% | 25.5 / 8.4 | 18 | L214F (TTG→TTC) | pstB‑II | phosphate ABC transporter ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base C (6/7); new base G (2/3); total (8/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACAGGGTATCGGTATCCCCGAATTCGACCAGTTTGCCCATGTACATGAACGCGGTGTAATCCGAAACACGCGCCGCTTGCTGCATGTTGTGGGTCACGATGACGATGGTGTACTTGGATTTCAATTCATAGATCAGTTCTTCGACCTTCAAGGTCGAGATCGGGTCCAGCGCCGAGCACGGTTCGTCAAGCAGCAGCACCTCCGGCTCCACGGCAATGGTGCGGGCGATGACCAGACGCTGCTGCTGGCCACCGGACAGGCCCAGCGCCGACTCGTGCAGG > NC_002947/6071791‑6072071 | aCAGGGTATCGGTATCCCCGAATTCGACCAGTTTGCCCATGTACATGAACGCGGTGTAATCCGAAACACGCGCCGCTTGCTGCATGTTGTGGGTCACGATGACGATGGTGTACTTGGATTTCAATTCATAGATCAGTTCTTCGACCTTCa < 1:94233/150‑1 (MQ=255) gtatcCCCGAATTCGACCAGTTTGCCCATGTACATGAACGCGGTGTAATCCGAAACACGCGCCGCTTGCTGCATGTTGTGGGTCACGATGACGATGGTGTACTTGGATTTCAATTCATAGATCAGTTCTTCGACCTTGAAGGTCgaagaa < 1:169091/150‑5 (MQ=255) tcCCCGAATTCGACCAGTTTGCCCATGTACATGAACGCGGTGTAATCCGAAACACGCGCCGCTTGCTGCATGTTGTGGGTCACGATGACGATGGTGTACTTGGATTTCAATTCATAGATCAGTTCTTCGACCTTCAAGGTCGAGATCggg < 2:122645/150‑1 (MQ=255) aCATGAACGCGGTGTAATCCGAAACACGCGCCGCTTGCTGCATGTTGTGGGTCACGATGACGATGGTGTACTTGGATTTCAATTCATAGATCAGTTCTTCGACCTTCAAGGTCGAGATCGGGTCCAGCGCCGAGCACGGTTCGTCAagca < 1:410393/150‑1 (MQ=255) tGAACGCGGTGTAATCCGAAACACGCGCCGCTTGCTGCATGTTGTGGGTCACGATGACGATGGTGTACTTGGATTTCAATTCATAGATCAGTTCTTCGACCTTCAAGGTCGAGATCGGGTCCAGCGCCGAGCACGGTTCGTCAagcagca < 2:49018/150‑1 (MQ=255) tCCGAAACACGCGCCGCTTGCTGCATGTTGTGGGTCACGATGACGATGGTGTACTTGGATTTCAATTCATAGCTCAGTTCTTCGACCTTCAAGGTCGAGATCGGGTCCAGCGCCGAGCACGGTTCGTCAAGCAGCAGCACCTCCGGCTcc > 2:602394/1‑150 (MQ=255) tCCGAAACACGCGCCGCTTGCTGCATGTTGTGGGTCACGATGACGATGGTGTACTTGGATTTCAATTCATAGATCAGTTCTTCGACCTTGAAGGTCgaag > 1:474683/1‑98 (MQ=255) tCCGAAACACGCGCCGCTTGCTGCATGTTGTGGGTCACGATGACGATGGTGTACTTGGATTTCAATTCATAGATCAGTTCTTCGACCTTGAAGGTCgaag < 2:474683/100‑3 (MQ=255) ggTCACGATGACGATGGTGTACTTGGATTTCAATTCATAGATCAGTTCTTCGACCTTCAAGGTCGAGATCGGGTCCAGCGcc < 1:581252/82‑1 (MQ=255) ggTCACGATGACGATGGTGTACTTGGATTTCAATTCATAGATCAGTTCTTCGACCTTCAAGGTCGAGATCGGGTCCAGCGcc > 2:581252/1‑82 (MQ=255) gtgtACTTGGATTTCAATTCATAGATCAGTTCTTCGACCTTCAAGGTCGAGATCGGGTCCAGCGCCGAGCACGGTTCGTCAAGCAGCAGCACCTCCGGCTCCACGGCAATGGTGCGGGCGATGACCAGACGCTGCTGCTGGCCACCGGAc > 1:386195/1‑150 (MQ=255) tACTTGGATTTCAATTCATAGATCAGTTCTTCGACCTTCAAGGTCGAGATCGGGTCCAGCGCCGAGCACGGTTCGTCAAGCAGCAGCACCTCCGGCTCCACGGCAATGGTGCGGGCGATGACCAGACGCTGCTGCTGGCCACCGGACAgg > 1:310551/1‑150 (MQ=255) ttGGATTTCAATTCATAGATCAGTTCTTCGACCTTCAAGGTCGAGATCGGGTCCAGCGCCGAGCACGGTTCGTCAAGCAGCAGCACCTCCGGCTCCACGGCAATGGTGCGGGCGATGACCAGACGCTGCTGCTGGCCACCg < 1:145115/141‑1 (MQ=255) ttGGATTTCAATTCATAGATCAGTTCTTCGACCTTCAAGGTCGAGATCGGGTCCAGCGCCGAGCACGGTTCGTCAAGCAGCAGCACCTCCGGCTCCACGGCAATGGTGCGGGCGATGACCAGACGCTGCTGCTGGCCACCg > 2:145115/1‑141 (MQ=255) aaTTCATAGATCAGTTCTTCGACCTTCAAGGTCGAGATCGGGTCCAGCGCCGAGCACGGTTCGTCAAGCAGCAGCACCTCCGGCTCCACGGCAATGGTGCGGGCGATGACCAGACGCTGCTGCTGGCCACCGGACAGGCCCAGCGCCGAc > 2:245912/1‑150 (MQ=255) aTCAGTTCTTCGACCTTCAAGGTCGAGATCGGGTCCAGCGCCGAGCACGGTTCGTCAAGCAGCAGCACCTCCGGCTCCACGGCAATGGTGCGGGCGATGACCAGACGCTGCTGCTGGCCACCGGACAGGCCCAGCGCCGACTCGTGCAgg < 1:354082/150‑1 (MQ=255) ccTTGAAGGTCGAGATCGGGTCCAGCGCCGAGCACGGTTCGTCAAGCAGCAGCACCTCCGGCTCCACGGCAATGGTGCGGGCGATGACCAGACgctgctgc > 1:334725/1‑101 (MQ=255) ccTTGAAGGTCGAGATCGGGTCCAGCGCCGAGCACGGTTCGTCAAGCAGCAGCACCTCCGGCTCCACGGCAATGGTGCGGGCGATGACCAGACgctgctgc < 2:334725/101‑1 (MQ=255) | ACAGGGTATCGGTATCCCCGAATTCGACCAGTTTGCCCATGTACATGAACGCGGTGTAATCCGAAACACGCGCCGCTTGCTGCATGTTGTGGGTCACGATGACGATGGTGTACTTGGATTTCAATTCATAGATCAGTTCTTCGACCTTCAAGGTCGAGATCGGGTCCAGCGCCGAGCACGGTTCGTCAAGCAGCAGCACCTCCGGCTCCACGGCAATGGTGCGGGCGATGACCAGACGCTGCTGCTGGCCACCGGACAGGCCCAGCGCCGACTCGTGCAGG > NC_002947/6071791‑6072071 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |