Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 413,107 C→G 15.0% A686A (GCC→GCG)  glnE → glutamate‑ammonia‑ligase adenylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947413,1070CG15.0% 67.2 / 5.8 28A686A (GCC→GCG) glnEglutamate‑ammonia‑ligase adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base C (13/10);  new base G (2/2);  total (16/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01

GCTGGCCCACAGCCTGCGCGTGGCCGCCTCGGAAATCAGCGGCAACCTGCCGTTGATGAAAGTCAGCGACTACCTGACCTGGCTGGCTGAAGCCATCCTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGAGCTCGGCCACGGTTCGGACCTGGACCTGGTCTTCATCCATGACGGCGATCCGCAGGCGGAAAC  >  NC_002947/412969‑413256
                                                                                                                                          |                                                                                                                                                     
gCTGGCCCACAGCCTGCGCGTGGCCGCCTCGGAAATCAGCGGCAACCTGCCGTTGATGAAAGTCAGCGACTACCTGACCTGGCTGGCTGAAGCCATCCTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCGGGCCACggtct                                                                                                                                            <  1:458309/150‑4 (MQ=255)
 cTGGCCCACAGCCTGCGCGTGGCCGCCTCGGAAATCAGCGGCAACCTGCCGTTGATGAAAGTCAGCGACTACCTGACCTGGCTGGCTGAAGCCATCCTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGCCaa                                                                                                                                           >  2:344262/1‑150 (MQ=255)
  tGGCCCACAGCCTGCGCGTGGCCGCCTCGGAAATCAGCGGCAACCTGCCGTTGATGAAAGTCAGCGACTACCTGACCTGGCTGGCTGAAGCCATCCTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGCCAAc                                                                                                                                          >  1:477246/1‑150 (MQ=255)
                              ggAAATCAGCGGCAACCTGCCGTTGATGAAAGTCAGCGACTACCTGACCTGGCTGGCTGAAGCCATCCTCTACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCTCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGa                                                                                                              >  1:192118/1‑150 (MQ=255)
                                        ggCAACCTGCCGTTGATGAAAGTCAGCGACTACCTGACCTGGCTGGCTGAAGCCATCCTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCGGGCCACggtc                                                                                                                                             <  1:84435/109‑3 (MQ=255)
                                        ggCAACCTGCCGTTGATGAAAGTCAGCGACTACCTGACCTGGCTGGCTGAAGCCATCCTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCGGGCCACggtc                                                                                                                                             >  2:84435/1‑107 (MQ=255)
                                        ggCAACCTGCCGTTGATGAAAGTCAGCGACTACCTGACCTGGCTGGCTGAAGCCATCCTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTtc                                                                                                    >  1:387300/1‑150 (MQ=255)
                                           aaCCTGCCGTTGATGAAAGTCAGCGACTACCTGACCTGGCTGGCTGAAGCCATCCTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTtcatc                                                                                                 <  1:595417/150‑1 (MQ=255)
                                                     tgatgaAAGTCAGCGACTACCTGACCTGGCTGGCTGAAGCCATCCTCGCCCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGtt                                                                                       <  1:344262/150‑1 (MQ=255)
                                                           aaGTCAGCGACTACCTGACCTGGCTGGCTGAAGCCATCCTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCa                                                                                 >  1:352927/1‑150 (MQ=255)
                                                                   gACTACCTGACCTGGCTGGCTGAAGCCATCCTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGggc                                                                         >  2:444624/1‑150 (MQ=255)
                                                                           gacctgGCTGGCTGAAGCCATCCTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGa                                                                 <  2:163699/150‑1 (MQ=255)
                                                                              ctggctggCTGAAGCCATCCTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGAGCt                                                              >  2:297207/1‑150 (MQ=255)
                                                                                ggctggttGACGCCACCCTCGACCAGGTGCTGGCCCTGGCCGGGCGCCAGACCGTTGCCCTCCACGGCCAACCCAAGCGCAGCGACGGCCGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGAGCTCg                                                            <  2:192118/150‑1 (MQ=255)
                                                                                       tgAAGCCATCCTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCCTTATCGGTTACGGCAAGATGGGCGGCCTGGAGCCCGGCCACgg                                                     >  2:294956/1‑150 (MQ=255)
                                                                                       tgAAGCCATCCTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGAGCTCGGCCACgg                                                     >  1:94226/1‑150 (MQ=255)
                                                                                              aTCCTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGAGCTCGGCCACGGTTCggac                                              >  2:3073/1‑150 (MQ=255)
                                                                                                 cTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCATCTTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGAGCTCGGCCACGGTTCggacctg                                           <  2:298890/150‑1 (MQ=255)
                                                                                                       cAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGAGCTCGGCCACGGTTCGgacctggacctg                                     <  1:297207/150‑1 (MQ=255)
                                                                                                              tggccctggacAGGTGCCATACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGCCAGCAGGTTCTCCGACCCTGTTTTCATCATTATCGGTTCCGCCACGATGAGCGGCCTGGCGCTCGGTCACGGTTCGGACCTGGACCTGGTCTTCa                              <  1:444624/150‑1 (MQ=255)
                                                                                                                gccctggccTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTtca                                                                                                   >  1:532872/1‑79 (MQ=255)
                                                                                                                gccctggccTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTtca                                                                                                   <  2:532872/79‑1 (MQ=255)
                                                                                                                 ccctggccTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGAGCTCGGCCACGGTTCGGACCTGGACCTGGTCTTCATcc                           >  2:601160/1‑150 (MQ=255)
                                                                                                                        ctggcGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGAGCTCGGCCACGGTTCGGACCTGGACCTGGTCTTCATCCATGACgg                    <  2:387300/150‑1 (MQ=255)
                                                                                                                           gcgcCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCAGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGAGCTCGGCCACGGTTCGGACCTGGACCTGGTCTGCATCCATGACGGCGa                 >  1:216758/1‑150 (MQ=255)
                                                                                                                               ttggccGTGGCGGGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGAGCTCGGCCACGGTTCGGACCTGGACCTGGTCTTCATCCATGACGGCGATCCg             >  1:321924/5‑150 (MQ=255)
                                                                                                                                aGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGAGCTCGGCCACGGTTCGgacctggacctg                                     <  1:540116/125‑1 (MQ=255)
                                                                                                                                aGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGAGCTCGGCCACGGTTCGgacctggacctg                                     >  2:540116/1‑125 (MQ=255)
                                                                                                                                          ccGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGAGCTCGGCCACGGTTCGGACCTGGACCTGGCCTTCATCCATGACGGCGATCCGCAGGCGGAAAc  >  1:272494/1‑150 (MQ=255)
                                                                                                                                          |                                                                                                                                                     
GCTGGCCCACAGCCTGCGCGTGGCCGCCTCGGAAATCAGCGGCAACCTGCCGTTGATGAAAGTCAGCGACTACCTGACCTGGCTGGCTGAAGCCATCCTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGAGCTCGGCCACGGTTCGGACCTGGACCTGGTCTTCATCCATGACGGCGATCCGCAGGCGGAAAC  >  NC_002947/412969‑413256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑