| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 467,104 | C→T | 19.0% | intergenic (‑34/+19) | PP_5445 ← / ← PP_t71 | hypothetical protein/tRNA‑Met |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 467,104 | 0 | C | T | 19.0% | 38.0 / 3.4 | 21 | intergenic (‑34/+19) | PP_5445/PP_t71 | hypothetical protein/tRNA‑Met |
| Reads supporting (aligned to +/- strand): ref base C (9/8); new base T (2/2); total (11/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01 | |||||||||||
GCGCTGGGTGACCGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCACCTGTGC > NC_002947/466957‑467241 | gcgcTGGGTGACCGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATcgc > 1:388039/1‑150 (MQ=255) gcTGGGTGACCGGCTTCTTGGTGGGGCTGATTTAGCTGCGATTCCGATTGGGGGCTTCTGCCTAGGTCATGCGCCAGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATcgcgc < 1:291370/150‑1 (MQ=255) ccGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTc > 2:187674/1‑150 (MQ=255) cGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCg > 1:36573/1‑150 (MQ=255) gggCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGcc < 2:36573/150‑1 (MQ=255) ggCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGccc < 2:388039/150‑1 (MQ=255) gCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCca > 2:474538/1‑150 (MQ=255) aTTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACg < 1:474538/150‑1 (MQ=255) cGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGa > 2:282263/1‑150 (MQ=255) tAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCaaa < 1:282263/150‑1 (MQ=255) tAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGAAATCGCGCGGCTTCGTCGTAACTGGTGGGCCCAGACGGATCCGAACCGTGGACCaaa < 2:179641/150‑1 (MQ=255) ttCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGa < 1:187674/150‑1 (MQ=255) tgGATGTACGCGCCCCTGCCCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCCTGGACCAAAAGATGATGAGGCCTCTGCTCTAACCAACTGAGCTATAgcc > 2:586304/1‑148 (MQ=255) ggATGTACGCGCTCCTGACCATTTATTTCTTGCGGGCATGAAACAGCCGCGGAATCGTGCGGCGTTGTCGTATCTGGTAGGCCCACACGGACTAGAACCGTGGGACAAAGGATTATGAGTCGTCTGCTCTAAACAACTGAACTATAGGcc > 2:31636/1‑150 (MQ=255) ggATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGcc < 1:590348/150‑1 (MQ=255) ttGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAggg > 2:150653/1‑150 (MQ=255) tGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCAcc > 1:52980/1‑150 (MQ=255) aaaGCCGCGCGATTGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAAc > 2:221398/1‑91 (MQ=255) aaaGCCGCGCGATTGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAAc < 1:221398/91‑1 (MQ=255) aaaGCCGCGCGATTGCGCGGCTTTGACGTAGCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCACCtgtg > 1:570687/1‑150 (MQ=255) aaGCCGCGCGATTGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCACCTGTGc < 1:233430/150‑1 (MQ=255) gcgcGATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCaaa < 2:353207/61‑1 (MQ=255) gcgcGATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCaaa > 1:353207/1‑61 (MQ=255) | GCGCTGGGTGACCGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCACCTGTGC > NC_002947/466957‑467241 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |