| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 471,070 | G→A | 23.9% | intergenic (‑111/+17) | PP_0386 ← / ← rpoD | sensory box protein/RNA polymerase sigma 70 factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 471,070 | 0 | G | A | 23.9% | 47.1 / 11.1 | 29 | intergenic (‑111/+17) | PP_0386/rpoD | sensory box protein/RNA polymerase sigma 70 factor |
| Reads supporting (aligned to +/- strand): ref base G (13/9); new base A (4/3); total (17/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCAGCAACAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGG > NC_002947/470929‑471213 | tCAGCAACAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTTAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCCAGGCCgggg < 1:2496/150‑1 (MQ=255) cAGCAACAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCCAGGCCGGGGt < 2:189012/150‑1 (MQ=255) cAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGTCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGagga > 2:305798/1‑150 (MQ=255) tCAGGTGGGCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCg > 2:330699/1‑150 (MQ=255) gtCTGAAGTCGAGTGTAGACGGGCATTGCCTCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCggg > 2:350808/1‑150 (MQ=255) tgtAGACGGGCATTGCCTCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTgc < 1:350808/150‑1 (MQ=255) gggCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGTAAAACCCCGGCCTGGGCCGGGGGTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGc > 2:104114/1‑150 (MQ=255) gCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGcc < 1:253820/141‑1 (MQ=255) gCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGcc > 2:253820/1‑141 (MQ=255) cGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGGCGGGTGGCGCAGCTTGCGCAAc < 2:36315/126‑1 (MQ=255) cGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGGCGGGTGGCGCAGCTTGCGCAAc > 1:36315/1‑126 (MQ=255) gggCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGa < 1:130569/136‑1 (MQ=255) gggCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGa > 2:130569/1‑136 (MQ=255) tCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTATTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCGGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCAAGCGTTACGTCa > 1:583722/1‑150 (MQ=255) ccACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCcttc < 2:212179/107‑1 (MQ=255) ccACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCcttc > 1:212179/1‑107 (MQ=255) aCTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTg < 1:330699/150‑1 (MQ=255) atgacaaaaCCCCGGCCCAGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGGCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGccc > 1:532346/6‑150 (MQ=255) aaGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCa > 1:96588/1‑150 (MQ=255) aaaaaCCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACct > 2:371632/1‑150 (MQ=255) aaaaCCCCGGCCCAGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACctc > 2:546428/1‑150 (MQ=255) aaaaCCCCGGCCCAGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGGCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACctc > 2:527896/1‑150 (MQ=255) aaCCCCGGCCCAGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCtt > 2:48536/1‑150 (MQ=255) aCCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTc < 1:305798/150‑1 (MQ=255) aCCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTc < 1:104114/150‑1 (MQ=255) cccGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGa > 1:160970/1‑150 (MQ=255) cccGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGa > 1:131833/1‑150 (MQ=255) cccGGCCCAGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGGCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGa < 2:532346/150‑1 (MQ=255) cGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAgg < 2:160970/150‑1 (MQ=255) | TCAGCAACAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGG > NC_002947/470929‑471213 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |