| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 81,984 | G→C | 15.5% | E98D (GAG→GAC) | PP_0071 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 81,984 | 0 | G | C | 15.5% | 82.1 / 8.1 | 33 | E98D (GAG→GAC) | PP_0071 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (17/10); new base C (2/3); total (20/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
CTATACCTGCGCAGCGCTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGAC‑TCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGCTTCTTCGCGGGTAAACCCGCTC > NC_002947/81844‑82130 | cTATACCTGCGCAGCGCTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCGTCGACTCGATGCCa < 2:323959/150‑1 (MQ=255) cgcAGCCCTTACCAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACGCGATGGCGTCGACTCGATGCCATCgagtatc < 2:273792/150‑7 (MQ=255) ggCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCa > 2:359016/1‑150 (MQ=255) aCAAAGCTTTAGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGAATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAgc < 2:73173/150‑1 (MQ=255) tttGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCCTCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTa > 1:66932/1‑150 (MQ=255) tttGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTa > 2:209689/1‑150 (MQ=255) tGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCCATCGCCGTCACGCCCAGCCTCCAAGGCACAGCGCTGCTATCGAGGCCGAC‑TCAGTACCTGTggg > 2:79479/1‑150 (MQ=255) gTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGAC‑TCa < 1:359016/150‑1 (MQ=255) gaagGCCGTGGCATGTTCTGGTAGAAACTGAGTGCGAATGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGAC‑TCAGTAcc > 2:376815/1‑150 (MQ=255) ccGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGc < 2:258014/105‑1 (MQ=255) ccGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGc > 1:258014/1‑105 (MQ=255) cGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGAC‑TCAGTACCTGTggg > 1:271752/1‑150 (MQ=255) tCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGAC‑TCAGTACCTGTGGGAGCGGGCGTGCCcgc > 2:576801/1‑150 (MQ=255) tCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGAC‑TCAGTACCTGTGGGAGCGGGCGTGCCcgc < 1:488846/150‑1 (MQ=255) tGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGAC‑TCAGTACCTGTGGGAGCGGGCGTGCCCGCGAac > 2:13272/1‑143 (MQ=255) tGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGAC‑TCAGTACCTGTGGGAGCGGGCGTGCCCGCGAac < 1:13272/143‑1 (MQ=255) agaTGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGAC‑TCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGt < 1:56638/150‑1 (MQ=255) agaTGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGAC‑CCAGTACCTGTGGGAGCGCGCGTTGCCGCGAACACCGGCAAAGCCGGt > 2:558949/1‑150 (MQ=255) gaTGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTA‑CCAAGCCAACGAAAGTACGTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTg > 2:172284/1‑150 (MQ=38) tgATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGAC‑TCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCgg > 2:473738/1‑125 (MQ=255) tgATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGAC‑TCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCgg < 1:473738/125‑1 (MQ=255) gtcgaCTCGATGCCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGAC‑TCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGCttcttc > 2:436050/5‑150 (MQ=255) gATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGAC‑TCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACAcc > 1:8730/1‑113 (MQ=255) gATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGAC‑TCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACAcc < 2:8730/113‑1 (MQ=255) tCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGAC‑TCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGc > 2:94887/1‑111 (MQ=255) tCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGAC‑TCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGCTTCTTCGCGGGt > 2:609274/1‑150 (MQ=255) tCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCACAGCGCTGCTATCGAGGCCGAC‑TCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGc < 1:94887/111‑1 (MQ=255) gATGGCGTCGACTCGATGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGAC‑TCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCcatccatc < 2:238434/128‑1 (MQ=255) gATGGCGTCGACTCGATGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGAC‑TCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCcatccatc > 1:238434/1‑128 (MQ=255) gATGGCGTCGACTCGATGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGAC‑TCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGCTTCTTCGCCGGTaa > 1:375991/1‑150 (MQ=38) gATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGAC‑TCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGCTTCTTCGCGGGCaa > 2:243803/1‑150 (MQ=255) tCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGAC‑TCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGCTTCTTCGCGGGTAAACCCGCt > 1:135776/1‑150 (MQ=255) cGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGAC‑TCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGCTTCTTCGCGGGTAAACCCGCTc < 1:376815/150‑1 (MQ=255) | CTATACCTGCGCAGCGCTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGAC‑TCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGCTTCTTCGCGGGTAAACCCGCTC > NC_002947/81844‑82130 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |