| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 711,631 | C→T | 12.9% | P476S (CCG→TCG) | ileS → | isoleucine‑‑tRNA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 711,631 | 0 | C | T | 12.9% | 72.3 / 4.3 | 31 | P476S (CCG→TCG) | ileS | isoleucine‑‑tRNA ligase |
| Reads supporting (aligned to +/- strand): ref base C (13/14); new base T (2/2); total (15/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.42e-01 | |||||||||||
AGCACCGGCGAGCCGCTGCGTGAGCGTGCGCTGAAAGCCATCGAAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATCCCGTTCTTCCTGCACAAGCAGACCGGCGATCTGCATCCGCGCACTGTCGAACTGATGGAAGAAGTGGCCAAGCGTGTCGAGCAGCAAGGCATCGAGGCCTGGTTCAAGCTGGACGCCGCCGAACTGCTGGGCGCTGAAGCCGA > NC_002947/711487‑711779 | aGCCCCGGCGAGCCGCTGCGTGAGCGTGCGCTGAAAGCCATCGAAGCCCCCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGCTCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATc < 2:143910/150‑1 (MQ=255) gCGAGCCGCTGCGTGAGCGTGCGCTGAAAGCCATCGAAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATCCCGttct > 2:225112/1‑150 (MQ=255) gCGAGCCGCTGCGTGAGCGTGCGCTGAAAGCCATCGAAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATCCCGttct < 2:320022/150‑1 (MQ=255) gCTGCGTGAGCGTGCGCTGAAAGCCATCGAAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATCCCGTTCTTCCTGca > 2:335915/1‑150 (MQ=255) cTGCGTGAGCGTGCGCTGAAAGCCATCGAAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATCCCGttctac < 2:532445/144‑3 (MQ=255) cTGCGTGAGCGTGCGCTGAAAGCCATCGAAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATCCCGttctac > 1:532445/1‑142 (MQ=255) gTGAGCGTGCGCTGAAAGCCATCGAAGACACCAAGTTCGTCCAGGCCCGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATCCCGTTCTTCCTGCACAAGc < 1:373768/150‑1 (MQ=255) cGTGCGCTGAAAGCCATCGAAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATCCCGTTCTTCCTGCACAAGCAGAcc < 1:188789/150‑1 (MQ=255) cGTGCGCTGAAAGCCATCGAAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGACCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATCCCGTTCTTCCTGCACAAGCAGAcc > 2:175324/1‑150 (MQ=255) gcgcTGAAAGCCATCGAAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATCCCGTTCTTCCTGCACAAGCAGACCGGc < 2:160435/150‑1 (MQ=255) cTGAAAGCCATCGAAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATCCCGtt > 2:582270/1‑125 (MQ=255) cTGAAAGCCATCGAAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGCGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATCCCGtt < 1:582270/125‑1 (MQ=255) tGAAAGCCATCGAAGACACCAAGTTCGTCCCGGCCTGTGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATCCCGTTCTTCCTGCACAAGCAGACCGGCGATc > 1:427499/1‑150 (MQ=255) cATCGAAGACACCAAGTTCGTCCCGGCCTGTGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATCCCGTTCTTCCTGCACAAGCAGACCGGCGATCTGCATcc < 2:427499/150‑1 (MQ=255) aaGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATCCCGTTCTTCCTGCACAAGCAGACCGGCGATCTGCATCCGCGCa > 1:574393/1‑150 (MQ=255) cAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGTCGATCCCGTTCTTCCTGCACAAGCAGAc > 1:364612/1‑123 (MQ=255) cAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGTCGATCCCGTTCTTCCTGCACAAGCAGAc < 2:364612/123‑1 (MQ=255) tCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATCCCGTTCTTCCTGCACAAGCAGACCGGCGATCTGCATCCGCGCACTGTCGAACTGa < 1:225112/150‑1 (MQ=255) cGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATCCCGTTCTTCCTGCACAAGCAGACCGGCGATCTGCATCCGCGCACTGTCGAACTGAt < 1:506528/150‑1 (MQ=255) tCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCCGCCCAACTGGGGTGTGCCGATCCCGTTCTTCCTGCACAAGCAGACCGGCGATCTGCATCCGCGCACTGTCGAACTGATgg > 1:197487/1‑150 (MQ=255) gCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCTGGACGGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATCCCGGTCTTCCTGCACAAGCAGACCGGCGATCTGCATCCGCGCACTGTCGAACTGATGGAAGAAGTGGCCa > 1:204986/1‑150 (MQ=255) tGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATCCCGTTCTTCCTGCACAAGCAGACCGGCGATCTGCATCCGCGCACTGTCGAACTGATGGAAGAAGTGGCCAAGCGTGTCGagc < 1:470639/150‑1 (MQ=255) cACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATCCCGTTCTTCCTTCACAAGCAGACCGGCGATCTGCATCCGCGCACGCTCGAACGGATGGAAGAAGTGGCCAAGCGTGTCGagcag > 1:500039/1‑150 (MQ=255) cGCCACACGCGCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATCCCGTTCTTCCTGCACAAGCAGACCGGCGATCTGCATCCGCGCACTGTCGAACTGATGGAAGAAGTGGCCAAGCGTGTCGAGCAGCAAGGCATCGa < 1:333000/150‑1 (MQ=255) gACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATCCCGTTCTTCCTGCACAAGCAGACCGGCGATCTGCATCCGCGCACTGTCGAACTGATGGAAGAAGTGGCCAAGCGTGTCGAGCAGCAAGGCATCGAGGCCTGGTTCAAg < 2:263702/150‑1 (MQ=255) gcgTCAGCGCAACTGGGGTGTGTCGATCCCGTTCTTCCTGCACAAGCAGACCGGCGATCTGCATCCGCGCACTGTCGAACTGATGGAAGAAGTGGCCAAGCGTGTCGAGCAGCAAg > 1:183116/1‑116 (MQ=255) gcgTCAGCGCAACTGGGGTGTGTCGATCCCGTTCTTCCTGCACAAGCAGACCGGCGATCTGCATCCGCGCACTGTCGAACTGATGGAAGAAGTGGCCAAGCGTGTCGAGCAGCAAg < 2:183116/116‑1 (MQ=255) gcgTCAGCGCAACTGGGGTGTGCCGATCCCGTTCTTCCTGCACAAGCAGACCGGCGATCTGCATCCGCGCACTGTCGAACTGATGGAAGAAGTGGCCAAGCGTGTCGAGCAGCAAGGCATCGAGGCCTGGTTCAAGCTGGACGCCGCCGa > 2:110919/1‑150 (MQ=255) gTCAGCGCAACTGGGGTGTGCCGATCCCGTTCTTCCTGCACAAGCAGACCGGCGATCTGCATCCGCGCACTGTCGAACTGATGGAAGAAGTGGCCAAGCGTGTCGAGCAGCAAGGCATCGAGGCCTGGTTCAAGCTGGACGCCGCCGAAc > 1:299569/1‑150 (MQ=255) tGGGGTGTGCCGATCCCGTTCTTCCTGCACAAGCAGACCGGCGATCTGCATCCGCGCACTGTCGAACTGATGGAAGAAGTGGCCAAGCGTGTCGAGCAGCAAGGCATCGAGGCCTGGTTCAAGCTGGACGCCGCCGAACTGCTGGGCGCt > 1:424797/1‑150 (MQ=255) gCCGATCCCGTTCTTCCTGCACAAGCAGACCGGCGATCTGCATCCGCGCACTGTCGAACTGATGGAAGAAGTGGCCAAGCGTGTCGAGCAGCAAGGCATCGAGGCCTGGTTCAAGCTGGACGCCGCCGAACTGCTGGGCGCTGAAGCCGa < 1:175324/150‑1 (MQ=255) ccGATCCCGTTCTTCCTGCACAAGCAGACCGGCGATCTGCATCCGCGCACTGTCGAACTGATGGAAGAAGTGGCCAAGCGTGTCGAGCAGCAAGGCATCGAGGCCTGGt < 1:98076/109‑1 (MQ=255) ccGATCCCGTTCTTCCTGCACAAGCAGACCGGCGATCTGCATCCGCGCACTGTCGAACTGATGGAAGAAGTGGCCAAGCGTGTCGAGCAGCAAGGCATCGAGGCCTGGt > 2:98076/1‑109 (MQ=255) | AGCACCGGCGAGCCGCTGCGTGAGCGTGCGCTGAAAGCCATCGAAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATCCCGTTCTTCCTGCACAAGCAGACCGGCGATCTGCATCCGCGCACTGTCGAACTGATGGAAGAAGTGGCCAAGCGTGTCGAGCAGCAAGGCATCGAGGCCTGGTTCAAGCTGGACGCCGCCGAACTGCTGGGCGCTGAAGCCGA > NC_002947/711487‑711779 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |