| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 966,599 | A→T | 14.8% | L41M (TTG→ATG) ‡ | PP_0828 ← | membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 966,599 | 0 | A | T | 14.8% | 65.4 / 5.9 | 27 | L41M (TTG→ATG) ‡ | PP_0828 | membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (16/7); new base T (2/2); total (18/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.52e-01 | |||||||||||
GCTGCGCGAACTGTTCACCAGCGCGCCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGA > NC_002947/966450‑966745 | gCTGCGCGAACTGTTCACCAGCGCGCCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCaa < 1:59333/150‑1 (MQ=255) cTGCGCGAACTGTTCACCAGCGCGCCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAg > 2:515197/1‑150 (MQ=255) cgAACTGTTCACAAACGCGCACCACAGGGTGCGAGCCTCCCACCAACGGCTGTAGGCGGTGCTGTTGCGAAAACTAACCAGCACCACCAGCGCTGAGCCCAGCGGGGTCCGCGGGATCAGTGGCAGGGTGAACTTCATGCTCGAGAACAg > 2:104713/1‑150 (MQ=255) aCTGTTCACCAGCGCGCCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCAt > 1:466850/1‑150 (MQ=255) ccAGCGCGCCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGaa < 2:422666/136‑1 (MQ=255) ccAGCGCGCCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGaa > 1:422666/1‑136 (MQ=255) cAGCGCGCCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGAt > 1:490810/1‑150 (MQ=255) cccACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCTTGAACAGCaag < 1:213918/134‑3 (MQ=255) cccACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCTTGAACAGCaag > 2:213918/1‑132 (MQ=255) gAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGa > 1:306978/1‑150 (MQ=255) atGCGAAAACTCACTAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGGGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGAtataa > 2:294059/2‑149 (MQ=255) gCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCATCATCCAGAACAGCGACCAGCCGATATAGcc < 2:466850/150‑1 (MQ=255) gAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGa > 1:351119/1‑150 (MQ=255) aaaCTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATg < 2:490810/150‑1 (MQ=255) aaaCTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATg < 2:351119/150‑1 (MQ=255) naCTCACCAGCACCACCAGCGCTGAGCCNAGCAGGGTNAGCGGGATCNGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATgg > 1:175654/2‑150 (MQ=255) aaCTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCGGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATgg > 2:65797/1‑150 (MQ=255) agtcaGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAg > 1:576142/4‑150 (MQ=255) accaGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGTCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAgg > 1:496231/1‑150 (MQ=255) accaGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTc < 1:35645/138‑1 (MQ=255) accaGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTc > 2:35645/1‑138 (MQ=255) ggTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGAc > 1:539925/1‑150 (MQ=255) tCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGt > 2:566703/1‑150 (MQ=255) gCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATc > 1:434416/1‑67 (MQ=255) gCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATc < 2:434416/67‑1 (MQ=255) tttcatGCTGTTCTTGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGt > 1:564343/6‑143 (MQ=255) tttcatGCTGTTCTTGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGt < 2:564343/138‑1 (MQ=255) tCAAGAACAGGATGAAATCAATAGGGACCAGCACATCCCAGATCAGCAGCCAGAACAGATACCACTCCATAGAGCCGCTCGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGa < 2:175654/150‑1 (MQ=255) | GCTGCGCGAACTGTTCACCAGCGCGCCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGA > NC_002947/966450‑966745 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |