Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	991,557	G→A	14.8%	G59D (GGT→GAT)	der →	GTPase Der

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	991,557	0	G	A	14.8%	59.6 / 4.6	27	G59D (GGT→GAT)	der	GTPase Der
Reads supporting (aligned to +/- strand):  ref base G (13/10);  new base A (2/2);  total (15/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-01

CCCGAACGTCGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAGATGATCGCCGAGCAC  >  NC_002947/991411‑991696                                                                                                                                                   |                                                                                                                                            cccGAACGTCGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTAt                                                                                                                                          >  1:425243/1‑150 (MQ=255)        gTCGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGt                                                                                                                                   <  2:489445/150‑1 (MQ=255)              aaaTCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGaa                                                                                                                             <  1:82636/150‑1 (MQ=255)                          ttCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGAc                                                                                                                 >  1:331695/1‑150 (MQ=255)                                         aaGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAg                                                                                                                            <  2:49797/124‑1 (MQ=255)                                         aaGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAg                                                                                                                            >  1:49797/1‑124 (MQ=255)                                              ccGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATg                                                                                                        <  1:1948/139‑1 (MQ=255)                                              ccGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATg                                                                                                        >  2:1948/1‑139 (MQ=255)                                                    tGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCTCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCAt                                                                                       >  2:253340/1‑150 (MQ=255)                                                              ggTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTgaa                                                                             <  2:331695/150‑1 (MQ=255)                                                                 gACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTgaagaa                                                                          <  2:425243/150‑1 (MQ=255)                                                                             cggACGCGTAACCGCGAGAATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATGGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGACCGAGCAGTCGCTCATGACCATTGAAGAAGCCGACTAtgta                                                              >  2:104111/3‑149 (MQ=255)                                                                                         ctccAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGtt                                                  <  1:253340/148‑1 (MQ=255)                                                                                                       tGCCAGCTGGCAGGGTCGTTACTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCggg                                    <  2:465666/150‑1 (MQ=255)                                                                                                        gCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCGCGTGCGGGt                                   >  1:538915/1‑150 (MQ=255)                                                                                                           aGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTTGGCATGGACGAAAAAATGGCCGAGCAGTCGCTGATGGCCATTGAAGAAGCCGACTATGGGCTGTTCCTGGTTGATGGCCGTGCGGGTATg                                >  1:193131/1‑150 (MQ=255)                                                                                                                     gTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAg                      >  2:279382/1‑150 (MQ=255)                                                                                                                           ccTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAga                <  1:279382/150‑1 (MQ=255)                                                                                                                                 tCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAGATGATCg          >  1:418327/1‑150 (MQ=255)                                                                                                                                 tCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAGATGATCg          >  1:124234/1‑150 (MQ=255)                                                                                                                                         gACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAGATGATCGCCGAGCAc  >  1:265678/1‑150 (MQ=255)                                                                                                                                          tcacCGGTGATACCACCGGTGTCGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGt                                                   <  1:514161/99‑1 (MQ=255)                                                                                                                                          tcacCGGTGATACCACCGGTGTCGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGt                                                   >  2:514161/2‑100 (MQ=255)                                                                                                                                            acCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAGATGATCGCCGAGc    <  1:530419/145‑1 (MQ=255)                                                                                                                                            acCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAGATGATCGCCGAGc    >  2:530419/1‑145 (MQ=255)                                                                                                                                            acCGGTGATACCACCGGTGTCGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAga                >  2:361839/1‑133 (MQ=255)                                                                                                                                            acCGGTGATACCACCGGTGTCGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAga                <  1:361839/133‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                            CCCGAACGTCGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAGATGATCGCCGAGCAC  >  NC_002947/991411‑991696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑