| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,090,037 | T→G | 22.8% | L298R (CTG→CGG) | fumC‑I → | class 2 fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,090,037 | 0 | T | G | 22.8% | 34.4 / 9.3 | 22 | L298R (CTG→CGG) | fumC‑I | class 2 fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base T (9/8); new base G (2/3); total (11/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
TCGAGTTCACGCCCGGCCAGAACCTGTTCGCACTGATCGGCTCGCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCAACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGGGCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTGATCCCGGAGGCCACTGCCATGGTCGCCGCCCAAGTGATTGGCAACGACGCGACCATCGCCGTCGCAGG > NC_002947/1089896‑1090181 | tCGAGTTCACGCCCGGCCAGAACCTGTTCGCACTGATCGGCTCGCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCAACGATCTGCGCTGGATGAACTCCGGCCAGCGGGCCggag < 1:297158/150‑3 (MQ=255)tCGAGTTCACGCCCGGCCAGAACCTGTTCGCACTGATCGGCTCGCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCAACGATCTGCGCTGGATGAACTCCGGCCAGCGGGCCggag < 1:51796/150‑3 (MQ=255) cGAGTTCACGCCCGGCCAGAACCTGTTCGCACTGATCGGCTCGCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCAACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCt < 1:368946/150‑1 (MQ=255) ccGGCCAGAACCTGTTCGCACTGATCGGCTCGCAGGGCACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCAACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGGGCGAAATCg > 2:347172/1‑150 (MQ=255) tGATCGGCTCGCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCAACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGGGCGAAATCGAGCTGCAAGCTCTGCAGCCgg > 1:453775/1‑150 (MQ=255) tGATCGGCTCGCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCAACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGGGCGAAATCGAGCTGCAAGCTCTGCAGCCgg < 2:453775/150‑1 (MQ=255) gACACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCAACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGGGCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCAtt < 2:422028/150‑2 (MQ=255) acacTGCCGTGGATTTGTCTGTCCTGCTTAAGGCCCCCGCTGGGCCGTTGGTGAAAATCGCCAACGATCTGCGCTGGATGACCTCCGGCCCGCTCGCCGGTCTGGGCGAAATCGCGCTGCACGCTCTGCAGCCGGGCTCTTCGATCATGc < 2:344571/150‑1 (MQ=255) cacTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCAACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGGGCGAAATCGAGCTGCAAGCTCTGCAGCCg < 1:392033/133‑1 (MQ=255) cacTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCAACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGGGCGAAATCGAGCTGCAAGCTCTGCAGCCg > 2:392033/1‑133 (MQ=255) tGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCAACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGGGCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGt > 2:512781/1‑150 (MQ=255) cAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCAACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGGGCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTGATc > 2:89631/1‑150 (MQ=255) aaaTCGCCAACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGGGCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTGATCCCGGAGGCCACTGCCATGGTCGCCGCCCAAg < 1:89631/150‑1 (MQ=255) tCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGGGCGAAATCGAGCTGCAAGCTCTGc > 1:499135/1‑62 (MQ=255) tCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGGGCGAAATCGAGCTGCAAGCTCTGc < 2:499135/62‑1 (MQ=255) tCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGGGCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTGATCCCGGCTCGCACTGCCATGGTCGCCGCCCAAGTGATTGGCAacga > 1:32280/1‑150 (MQ=255) gcTGGATGAACTCCGGCCCGCTGGCCGGTCTGGGCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTGATCCCGGAGGCCACTGCCATGGTCGCCGCCCAAGTGATTGGCAACGACGCGa > 1:35692/1‑150 (MQ=255) ccataccGGCCAGCGGGCCGGTCTGGGCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTGATCCCGGAGGCCACTGCCATGGTCGCCGCCCAAGTGATTGGCAACGACGCGACCATCGc > 2:164906/6‑150 (MQ=255) gaccGGCCAGCGGGCCGGTCTGGGCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCgg < 1:71303/84‑1 (MQ=38) gaccGGCCAGCGGGCCGGTCTGGGCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCgg > 2:71303/3‑86 (MQ=38) ggCCCGCTGGCCGGTCTGGGCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTGATCCCGGAGGCCACTGCCATGGTCGCCGCCCAAGTGATTGGCAACGACGCGACCATCGCCGTCGCa > 2:2196/1‑150 (MQ=255) cccGCTGGCCGGTCTGGGCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTGATCCCGGAGGCCACTGCCATGGTCGCCGCCCAAGTGATTGGCAACGACGCGACCATCGCCGTCGCAgg < 1:463657/150‑1 (MQ=255) | TCGAGTTCACGCCCGGCCAGAACCTGTTCGCACTGATCGGCTCGCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCAACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGGGCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTGATCCCGGAGGCCACTGCCATGGTCGCCGCCCAAGTGATTGGCAACGACGCGACCATCGCCGTCGCAGG > NC_002947/1089896‑1090181 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |