| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 126,115 | A→G | 13.2% | intergenic (+25/+25) | PP_0120 → / ← thrB | zinc ABC transporter/homoserine kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 126,115 | 0 | A | G | 13.2% | 90.4 / 5.6 | 38 | intergenic (+25/+25) | PP_0120/thrB | zinc ABC transporter/homoserine kinase |
| Reads supporting (aligned to +/- strand): ref base A (17/16); new base G (3/2); total (20/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01 | |||||||||||
GCCTTGCCGAGCTGGATGCCTTGGGTGGCACCGATCCGGTGGATGGCAAAGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCG‑‑CGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAACTCGCTCGGATCGTGGATCATCACATCCATCCCGGCGAACGACTCGGCCGCAATCAGGC > NC_002947/125978‑126258 | gCCTTGCCGAGCTGGATGCCTTGGGTGGCACCGATCCGGTGGATGGCAAAGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCG‑‑CGGGCGTGCCCGCgaa > 1:194559/1‑148 (MQ=255)gCCTTGCCGAGCTGGATGCCTTGGGTGGCACCGATCCGGTGGATGGCAAAGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCG‑‑CGGGCGTGCCCGCgaa < 2:194559/148‑1 (MQ=255)gCCTTGCCGAGCTGGATGCCTTGGGTGGCACCGATCCGGTGGATGGCAAAGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCG‑‑CGGGCACGCCCGCgaaga > 1:558301/1‑150 (MQ=255) tGCCGAGCTGGATGCCTTGGGTGGCACCGATCCGGTGGATGGCAAAGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCG‑‑CGGGCACGCCCGCGAAGAAGCa > 2:419206/1‑150 (MQ=255) agatGGATGCCTTGGGTGGCACCGATCCGGTGGATGGCAAAGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCG‑‑CGGGCACGCCCGCGAAGAAGCAGAcgc < 2:21008/147‑1 (MQ=255) aTGCCTTGGGTGGCACCGATCCGGTGGATGGCAAAGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCG‑‑CGGGCACGCCCGCGAAGAAGCAGACGCGGTCTa > 2:400914/1‑150 (MQ=255) tGGCACCGATCCGGTGGATGGCAAAGGGTATGAGCGCTTTCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCG‑‑CGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAAc < 2:63889/150‑1 (MQ=255) tGGCACCGATCCGGTGGATGGCAAAGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCG‑‑CGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAAc > 2:424279/1‑150 (MQ=255) aCCGATCCGGTGGATGGCAAAGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCG‑‑CGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACATCGCGAACGGCa > 1:241791/1‑150 (MQ=255) aCCGATCCGGTGGATGGCAAAGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCG‑‑CGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCa > 2:144553/1‑150 (MQ=255) aCCGATCCGGTGGATGGCAAAGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCG‑‑CGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCa < 1:144553/150‑1 (MQ=255) ggTGGATGGCAAAGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCG‑‑CGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAAt < 1:184816/150‑1 (MQ=255) aaGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCG‑‑CGGGCGTGCCCGCgaaggg < 2:451895/103‑3 (MQ=255) aaGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCG‑‑CGGGCGTGCCCGCgaaggg > 1:451895/1‑101 (MQ=255) aGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCG‑‑CGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTgg < 1:400914/150‑1 (MQ=255) aGCGCTTGCTGCAGAGGCTTGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTAG‑‑AGCGCACGCGGATACCATCGCTCGGCCACGCCCGCGAATAAGCAGACGCCGTTCACAGATCTCACGGCAGGTGCAATGCCACCTGCTGGCGCTgcgc < 1:141039/150‑1 (MQ=255) tGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCG‑‑CGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCAc > 2:396960/1‑150 (MQ=255) tGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCG‑‑CGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCAc > 2:114484/1‑150 (MQ=255) tGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCG‑‑CGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAActc < 2:558301/150‑1 (MQ=255) gggCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCG‑‑CGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACctgctg > 2:56395/1‑122 (MQ=255) gggCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCG‑‑CGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACctgctg < 1:56395/122‑1 (MQ=255) cTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCG‑‑CGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAACTCGCTCGGATCGt > 2:292150/1‑150 (MQ=255) aGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCG‑‑CGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTgcgc > 2:128242/1‑116 (MQ=255) aGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCG‑‑CGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTgcgc < 1:128242/116‑1 (MQ=255) cTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCG‑‑CGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAACTCGCTCGGATCGTGGatcatc > 2:537839/1‑150 (MQ=255) cTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCG‑‑CGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAACTCGCTCGGATCGTGGATCATCAcat < 2:539947/150‑1 (MQ=255) aGTTGTAAGCCTGTGCCGGCCCCTTCG‑‑CGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAACTCGCTCGGATCGTGGATCATCACATCCATccc < 2:603221/150‑1 (MQ=255) aGTTGTAAGCCTGTGCCGGCCCCTTCG‑‑CGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAACTCGCTCGGATCGTGGATCATCACATCCATccc < 1:292150/150‑1 (MQ=255) ttGTAAGCCTGTGCCGGCCCCTTCG‑‑CGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAACTCGCTCGGATCGTGGATCATCACATCCATCCCgg > 2:267683/1‑150 (MQ=255) gTAAGCCTGTGCCGGCCCCTTCG‑‑CGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAACTCGCTCGGATCGTGGATCATCACATCCATCCCGGCg > 1:271683/1‑150 (MQ=255) aGCCTGTGCCGGCCCCTTCG‑‑CGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAACTCGCTCGGATCGTGGATCATCACATCCATCCCGGCGAAc > 1:361648/1‑150 (MQ=255) gCCTGTGCCGGCCCCTTCG‑‑CGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAACTCGCTCGGATCGTGGatc > 2:80775/1‑128 (MQ=255) gCCTGTGCCGGCCCCTTCG‑‑CGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAACTCGCTCGGATCGTGGatc < 1:80775/128‑1 (MQ=255) ccTGTGCCGGCCCCTTCG‑‑CGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAACTCGCTCGGATCGTGGATCATCACATCCATCCCGGCGAACGa < 1:424279/150‑1 (MQ=255) gCCCCTTCG‑‑CGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAACTCGCTCGGATCGTGGATCATCACATCCATCCCGGCGAACGACTCGGCCGc > 1:478145/1‑150 (MQ=255) cTTCG‑‑CGGGCGTGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAACTCGCTCGGATCGTGGATCATCACATCCATCCCGGCGAACGACTCGGCCGCAATc > 2:307035/1‑150 (MQ=255) cTTCG‑‑CGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAACTCGCTCGGATCGTGGATCATCACATCCATCCCGGCGAACGACTCGGCCGCAATc < 2:139872/150‑1 (MQ=255) g‑‑cgGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAACTCGCTCGGATCGTGGATCATCACATCCATCCCGGCGAACGACTCGGCCGCAATCAGgc < 1:396960/150‑1 (MQ=255) | GCCTTGCCGAGCTGGATGCCTTGGGTGGCACCGATCCGGTGGATGGCAAAGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCG‑‑CGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAACTCGCTCGGATCGTGGATCATCACATCCATCCCGGCGAACGACTCGGCCGCAATCAGGC > NC_002947/125978‑126258 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |