| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,182,864 | A→T | 16.0% | intergenic (+25/+69) | tadA → / ← mltF | tRNA adenosine(34) deaminase TadA/membrane‑bound lytic murein transglycosylase F |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,182,864 | 0 | A | T | 16.0% | 59.6 / 5.8 | 25 | intergenic (+25/+69) | tadA/mltF | tRNA adenosine(34) deaminase TadA/membrane‑bound lytic murein transglycosylase F |
| Reads supporting (aligned to +/- strand): ref base A (11/10); new base T (2/2); total (13/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01 | |||||||||||
GCCAGGGGCAGTTCTTTGGCCAAGGCTTTCTCAACCACCGGGTGATGGTGGAGGGCGGGGTGCTGGCGCAAGAGTGCGGGCAGATCCTCAGCGACTTCTTCAAGGCCCGCCGGGCCAAGGCTTGAGCTGCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGTTCTGCGCAAATCTTGTAGGCGCGGGTTTGCCCGCGAAGAGGTCCTGACAGGCTTACATCGGCGACGACTTATCCGCTGGCTTGTCCTTGTTGACCCCGGGCACATGCAGGTTGCCCTC > NC_002947/1182715‑1182998 | gCCAGGGGCAGTTCTTTGGCCAAGGCTTTCTCAACCACCGGGTGATGGTGGAGGGCGGGGTGCTGGCGCAAGAGTGCGGGCAGATCCTCAGCGACTTCTTCAAGGCCCGCCGGGCCAAGGCTTGAGCTGCACCGGCCTCTTCGCGGGCaa < 1:72506/150‑1 (MQ=255) ccAGGGGCAGTTCTTTGGCCAAGGCTTTCTCAACCACCGGGTGATGGTGGAGGGCGGGGTGCTGGCGCAAGAGTGCGGGCAGATCCTCAGCGACTTCTTCAAGGCCCGCCGGGCCAAGGCTTGAGCTGTACCGGCCTCTTCGCGGGCAAg > 2:296088/1‑150 (MQ=255) cAAGGCTTTCTCAACCACCGGGTGATGGTGGAGGGCGGGGTGCTGGCGCAAGAGTGCGGGCAGATCCTCAGCGACTTCTTCAAGGCCCGCCGGGCCAAGGCTTGAGCTGCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGTTCTg > 1:375033/1‑150 (MQ=255) aaGGCTTTCTCAACCACCGGGTGATGGTGGAGGGCGGGGTGCTGGCGCAAGAGTGCGGGCAGATCCTCAGCGACTTCTTCAAGGCCCGCCGGGCCAAGGCTTGAGCTGCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGTTCTgc < 2:492472/150‑1 (MQ=255) ggTGATGGTGGAGGGCGGGGTGCTGGCGCAAGAGTGCGGGCAGATCCTCAGCGACTTCTTCAAGGCCCGCCGGGCCAAGGCTTGAGCTGCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGTTCTGCGCAAATCTTGTAGGCGCgg > 1:590119/1‑150 (MQ=255) tggAGGGCGGGGTGCTGGCGCAAGAGTGCGGGCAGATCCTCAGCGACTTCTTCAAGGCCCGCCGGGCCAAGGCTTGAGCTGCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGTTCTGCGCAAATCTTGTAGGCGCGGGTTTGccc > 2:123264/1‑150 (MQ=255) aGGGCGGGGTGCTGGCGCAAGAGTGCGGGCAGATCCTCAGCGACTTCTTCAAGGCCCGCCGGGCCAAGGCTTGAGCTGCACCGGCCTCTTCGCGGGCAAACCCGCGCCTACAAGATTTGCGCAGAACCTGTAGGAGCGGGCTTGCCcgcg < 1:601391/150‑1 (MQ=21) cGGGGTGCTGGCGCAAGAGTGCGGGCAGATCCTCAGCGACTTCTTCAAGGCCCGCCGGGCCAAGACTTGAGCTGCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGTTCTGCGCAAATCTTGTAGGCGCGGGTTTGCCCGCGAaga < 2:333733/150‑1 (MQ=255) cgcAAGAGTGCGGGCAGATCCTCAGCGACTTCTTCAAGGCCCGCCGGGCCAAGGCTTGAGCTGCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGTTCTGCGCAAATCTTGTAGGCGCGGGTTTGCCCGCGAAGAGGTCCTGACAg < 2:516737/150‑1 (MQ=255) gcAAGAGTGCGGGCAGATCCTCAGCGACTTCTTCAAGGCCCGCCGGGCCAAGGCTTGAGCTGCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTAAAGGTTGTGCGCAAATCTTGTAGGCGCGGGTTTGCCCGCGAAGAGATCCTGCCAgg > 1:183338/1‑150 (MQ=255) ggCAGATCCTCAGCGACTTCTTCAAGGCCCGCCGGGCCAAGGCTTGAGCTGCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGTTCTGCGCAAATCTTGTAGGCGCGGGTTTGCCCGCGAAGAGGTCCTGACAGGCTTACATCGGc < 1:111470/150‑1 (MQ=255) ggCAGATCCTCAGCGACTTCTTCAAGGCCCGCCGGGCCAAGGCTTGAGCTGCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGTTCTGCGCAAATCTTGTAGGCGCGGGTTTGCCCGCGAAGAGGTCCTGACAGGCTTACATCGGc < 1:111435/150‑1 (MQ=255) tCAGCGACTTCTTCAAGGCCCGCCGGGCCAAGGCTTGAGCTGCACCGGCCTCTTCGCGGGCAAGCCCGCTc < 2:363304/71‑1 (MQ=255) tCAGCGACTTCTTCAAGGCCCGCCGGGCCAAGGCTTGAGCTGCACCGGCCTCTTCGCGGGCAAGCCCGCTc > 1:363304/1‑71 (MQ=255) tCAGCGACTTCTTCAAGGCCCGCCGGGCCAAGGCTTGAGCTGCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGTTCTGCGCAAATCTTGTAGGCGCGGGTTTGCCCGCGAAGAGGTCCTGACAGGCTTACATCGGCGACGACTTa > 1:491656/1‑150 (MQ=255) tcttcAAGGCCCGCCGGGCCAAGGCTTGAGCTGCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGTTCTGCGCAAATCTTGTAGGCGCGGGTTTGCCCGCGAAGAGGTCCTGACAGACTTACATCGGCGACGACTTATCCGCTGGc < 2:375033/150‑1 (MQ=255) cttcAAGGCCCGCCGGGCCAAGGCTTGAGCTGCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGTTCTGCGCAAATCTTGTAGGCGCGGGTTTGCCCGCGAagag > 1:448830/1‑109 (MQ=255) cttcAAGGCCCGCCGGGCCAAGGCTTGAGCTGCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGTTCTGCGCAAATCTTGTAGGCGCGGGTTTGCCCGCGAagag < 2:448830/109‑1 (MQ=255) ttcAAGGCCCGCCGGGCCAAGGCTTGAGCTGCACCGGCCTCTTCGCGGGCTTGCCCGcgaa > 1:373701/1‑58 (MQ=21) ttcAAGGCCCGCCGGGCCAAGGCTTGAGCTGCACCGGCCTCTTCGCGGGCTTGCCCGcgaa < 2:373701/61‑4 (MQ=21) attcccGCCGGGCCAAGGCTTGAGCTGCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGTTCTGCGCAAATATTGTAGGCGCGGGTTTGCCCGCGAAGAGGTCCTGACAGGCTTACATCGGCGACGACTTATCCGCTGGCTTGTcc > 1:547523/4‑150 (MQ=255) gCCAAGGCTTGAGCTGCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGTTCTGCGCAAATCTTGTAGGCGCGGGTTTGCCCGCGAAGAGGTCCTGACAGGCTTACATCGGCGACGACTTATCCGCTGGCTTGTCCTTGTTGAcccc < 2:590119/150‑1 (MQ=255) ccGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGTTCTGCGCAAATCTTGTAGGCGCGGGTTTGCCCGCGAAGAGGTCCTGACAGGCTTACATCGGCGACGACTTATCCGCTGGCTTGTCCTTGTTGACCCCGGGCACATGCAGGTTGcc > 2:548282/1‑150 (MQ=255) gCCTCTTCGCGGGCAAGCCCGCTCCTACAGGTTCTGCGCAAATCTTGTAGGCGCGGGTTTGCCCGCGAAGAGGTCCTGACAGGCTTACATCGGCGACGACTTATCCGCTGGCTTGTCCTTGTTGACCCCGGGCACATGCAGGTTGCCCTc > 2:608128/1‑150 (MQ=255) gtaggagcgGGCTTGCCCGCTCCTACAGGTTCTGCGCAAATCTTGTAGGCGCGGGTTTGCCCGCGAAGAGGTCCTGACAGGCTTACATCGGCGACGACTTATCCGCTGGCTTGTCCTTGTTGACCCCGGGCACATGCAGGTTGCCCTc > 1:492915/7‑148 (MQ=255) gtaggagcgGGCTTGCCCGCTCCTACAGGTTCTGCGCAAATCTTGTAGGCGCGGGTTTGCCCGCGAAGAGGTCCTGACAGGCTTACATCGGCGACGACTTATCCGCTGGCTTGTCCTTGTTGACCCCGGGCACATGCAGGTTGCCCTc < 2:492915/142‑1 (MQ=255) | GCCAGGGGCAGTTCTTTGGCCAAGGCTTTCTCAACCACCGGGTGATGGTGGAGGGCGGGGTGCTGGCGCAAGAGTGCGGGCAGATCCTCAGCGACTTCTTCAAGGCCCGCCGGGCCAAGGCTTGAGCTGCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGTTCTGCGCAAATCTTGTAGGCGCGGGTTTGCCCGCGAAGAGGTCCTGACAGGCTTACATCGGCGACGACTTATCCGCTGGCTTGTCCTTGTTGACCCCGGGCACATGCAGGTTGCCCTC > NC_002947/1182715‑1182998 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |