| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,219,156 | C→T | 16.3% | D229D (GAC→GAT) ‡ | PP_1064 → | alpha/beta family hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,219,156 | 0 | C | T | 16.3% | 63.7 / 5.1 | 30 | D229D (GAC→GAT) ‡ | PP_1064 | alpha/beta family hydrolase |
| Reads supporting (aligned to +/- strand): ref base C (12/13); new base T (2/3); total (14/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGCGTTGGGCGTGCGCCAGTTTCATGTATTGGGTGTGAGCATGGGCGGCATGATTGCCCAGCACCTGGCGGCGATGGCGCCCGAGCGTGTGCGCAGCCTGACGCTGGTCATGTCCAGTTCAGGGGCGGCGGGGCTCCCGGCGCCGGACCCGGCGCTGGTGCAGTTGCTGGCCCGGCGCAGCGCACCGAACCGTGAAGTGGCAATCGAGCAGCAGGCCGACCTGCTGGCGGCCTTGGGCAGCCCTGAGGTACGGGACGACCGCGAGGTGTTGCTGCACCAGGCGGCGCAGGCCTACGA > NC_002947/1219008‑1219305 | aCGCGTTGGGCGTGCGCCAGTTTCATGTATTGGGTGTGAGCATGGGCGGCATGATTGCCCAGCACCTGGCGGCGATGGCGCCCGAGCGTGTGCGCAGCCTGACGCTGGTCATGTCCAGTTCAGGGGCGGCGGGGCTCCCGGCGCCGGAcc > 1:395870/1‑150 (MQ=255) gCGTGCGCCAGTTTCATGTATTGGGTGTGAGCATGGGCGGCATGATTGCCCAGCACCTGGCGGCGATGGCGCCCGAGCGTGTGCGCAGCCTGACGCTGGTCATGTCCAGTTCAGGGGCGGCGGGGCTCCCGGCGCCGGACCCGGCGCTgg > 1:510946/1‑150 (MQ=255) cGTGCGCCAGTTTCATGTATTGGGTGTGAGCATGGGCGGCATGATTGCCCAGCACCTGGCGGCGATGGCGCCCGAGCGTGTGCGCAGCCTGACGCTGGTCATGTCCAGTTCAGGGGCGGCGGGGCTCCCGGCGCCGGGTCCGGCGCCggg < 1:327063/150‑2 (MQ=255) cGTGCGCCAGTTTCATGTATTGGGTGTGAGCATGGGCGGCATGATTGCCCAGCACCTGGCGGCGATGGCGCCCGAGCGTGTGCGCAGCCTGACGCTGGTCATGTCCAGTTCAGGGGCGGCGGGGCTCCCGGCGCCGGACCCGGCGCTGGt < 2:510946/150‑1 (MQ=255) aTGTATTGGGTGTGAGCATGGGCGTCATGATTGCCCAGCACCTGGCGGCGATGGCGCCCGAGCGTGTGCGCAGCCTGACGCTGGTCATGTCCAGTTCAGGGGCGGCGGGGCTCCCGGCGCCGGACCCGGCGCTGGTGCAGTTGCTGGccc > 2:24587/1‑150 (MQ=255) cATGGGCGGCATGATTGCCCAGCACCTGGCGGCGATGGCGCCCGAGCGTGTGCGCAGCCTGACGCTGGTCATGTCCAGTTCAGGGGCGGCGGGGCTCCCGGCGCCGGGTCCGgcgccggg > 1:132091/1‑116 (MQ=255) cATGGGCGGCATGATTGCCCAGCACCTGGCGGCGATGGCGCCCGAGCGTGTGCGCAGCCTGACGCTGGTCATGTCCAGTTCAGGGGCGGCGGGGCTCCCGGCGCCGGGTCCGGCGCCggg < 2:132091/120‑2 (MQ=255) ggcATGATTGCCCAGCACCTGGCGGCGATGGCGCCCGAGCGTGTGCGCAGCCTGACGCTGGTCATGTCCAGTTCAGGGGCGGCGGGGCTCCCGGCGCCGGACCCGGCGCTGGTGCAGTTGCTGGCCCGGCGCAGCGCACCGAACCGTGaa < 2:203240/150‑1 (MQ=255) aTGATTGCCCAGCACCTGGCGGCGATGGCGCCCGAGCGTGTGCGCAGCCTGACGCTGGTCATGTCCAGTTCAGGGGCGGCGGGGCTCCCGGCGCCGGACCCGGCGCTGGTGCAGTTGCTGGCCCGGCGCAGCGCACCGAACCGTGAAGTg < 1:429402/150‑1 (MQ=255) gCCCAGCACCTGGCGGCGATGGCGCCCGAGCGTGTGCGCAGCCTGACGCTGGTCATGTCCAGTTCAGGGGCGGCGGGGCTCCCGGCGCCGGACCCGGCGCTGGTGCAGTTGCTGGCCCGGCGCAGCGCACCGAACCGTGAAGTGGCAATc < 1:498721/150‑1 (MQ=255) gCCCAGCACCTGGCGGCGATGGCGCCCGAGCGTGTGCGCAGCCTGACGCTGGTCATGTCCAGTTCAGGGGCGGCGGGGCTCCCGGCGCCGGACCCGGCGCTGGTGCAGTTGCTGGCCCGGCGCAGCGCACCGAACCGTGAAGTGGCAATc < 1:253356/150‑1 (MQ=255) tGGCGGCGATGGCGCCCGAGCGTGTGCGCAGCCTGACGCTGGTCATGTCCAGTTCAGGGGCGGCGGGGCTCCCGGCGCCGGACCCGGCGCTGGTGCAGTTGCTGGCCCGGCGCAGCGCACCGAACCGTGAAGTGGCAATCGAGCAGCAgg > 2:163955/1‑150 (MQ=255) ggcggcGATGGCGCCCGAGCGTGTGCGCAGCCTGACGCTGGTCATGTCCAGTTCAGGGGCGGCGGGGCTCCCGGCGCCGGACCCGGCGCTGGTGCAGTTGCTGGCCCGGCGCAGCGCACCGAACCGTGAAGTGGCAATCGAGCAGCAGGc < 1:163955/150‑1 (MQ=255) gcggcgATGGCGCCCGAGCGTGTGCGCAGCCTGACGCTGGTCATGTCCAGTTCAGGGGCGGCGGGGCTCCCGGCGCCGGACCCGGCGCTGGTGCAGTTGCTGGCCCGGCGCAGCGCACCGAACCGTGAAGTGGCAATCGAGCAGCAGGcc > 1:255301/1‑150 (MQ=255) gATGGCGCCCGAGCGTGTGCGCAGCCTGACGCTGGTCATGTCCAGTTCAGGAGCGGCGGGGCTCCCGGCGCCGGACCCGGCGCTGGTGCAGTTGCTGGACCGGCGCAGCGCACCGAACCGTCAGGTGGCAAGCGAACGACAGGCCTACct > 2:261161/1‑150 (MQ=255) gcCCGAGCGTGTGCGCAGCCTGACGCTGGTCATGTCCAGTTCAGGGGCGGCGGGGCTCCCGGCGCCGGACCCGGCGCTGGTGCAGTTGCTGGCCCGGCGCAGCGCACCGAACCGTGAAGTGGCAATCGAGCAGCAGGTCGGCctgctgct < 2:381423/150‑3 (MQ=255) cccGAGCGTGTGCGCAGCCTGACGCTGGTCATGTCCAGTTCAGGGGCGGCGGGGCTCCCGGCGCCGGACCCGGCGCTGGTGCAGTTGCTGGCCCGGCGCAGCGCACCGAACCGTGAAGTGGCAATCGAGCAGCAGGCCGACCTGCTggcg > 2:126181/1‑150 (MQ=255) gACGCTGGTCATGTCCAGTTCAGGGGCGGCGGGGCTCCCGGCGCCGGACCCGGCGCTGGTGCAGTTGCTGGCCCGGCGCAGCGCACCGAACCGTGAAGTGGCAATCGAGCAGCAGGCCGACCTGCTGGCGGCCt > 2:432093/1‑134 (MQ=255) gACGCTGGTCATGTCCAGTTCAGGGGCGGCGGGGCTCCCGGCGCCGGACCCGGCGCTGGTGCAGTTGCTGGCCCGGCGCAGCGCACCGAACCGTGAAGTGGCAATCGAGCAGCAGGCCGACCTGCTGGCGGCCt < 1:432093/134‑1 (MQ=255) tCCAGTTCAGGGGCGGCGGGGCTCCCGGCGCCGGACCCGGCGCTGGTGCAGTTGCTGGCCCGGCGCAGCGCACCGAACCGTGAAGTGGCAATCGAGCAGCAGGCCGACCTGCTGGCGGCCTTGGGCAGCCCTGAGGTACGGGACGACcgc > 2:318191/1‑150 (MQ=255) tCCAGTTCAGGGGCGGCGGGGCTCCCGGCGCCGGACCCGGCGCTGGTGCAGTTGCTGGCCCGGCGCAGCGCACCGAACCGTGAAGTGGCAATCGAGCAGCAGGCCGACCTGCTGGCGGCCTTGGGCAGCCCTGAGGTACGGGACGACcgc > 2:447252/1‑150 (MQ=255) gTTCAGGGGCGGCGGGGCTCCCGGCGCCGGACCCGGCGCTGGTGCAGTTGCTGGCCCGGCGCAGCGCACCGAACCGTGAAGTGGCAATCGAGCAGCAGGCCGACCTGCTGGCGGCCTTGGGCAGCCCTGAGGTACGGGACGACCGCGAgg > 2:400005/1‑150 (MQ=255) ttCAGGGGCGGCGGGGCTCCCGGCGCCGGACCCGGCGCTGGTGCAGTTGCTGGCCCGGCGCAGCGCACCGAACCGTGAAGTGGCAATCGAGCAGCAGGCCGACCTGCTGGCGGCCTTGGGCAGCCCTGAGGTACGGGACGACCGCGAGgt < 2:126699/150‑1 (MQ=255) gggCGGCGGGGCTCCCGGCGCCGGACCCGGCGCTGGTGCAGTTGCTGGCCCGGCGCAGCGCACCGAACCGTGAAGTGGCAATCGAGCAGCAGGCCGACCTGCTGGCGGCCTTGGGCGGCCCTGAGGTACGGGACGACCCCGAGGTGGtgc > 2:195380/1‑150 (MQ=255) gCTCCCGGCGCCGGACCCGGCGCTGGTGCAGTTGCTGGCCCGGCGCAGCGCACCGAACCGTGAAGTGGCAATCGAGTAGCAGGCCGACCTGCTGGCGGCCTTGGGCAGCCCTGAGGTACGGGACGACCGCGAGGTGTTGCTGCACCAggc < 1:64405/150‑1 (MQ=255) ccGGCGCCGGACCCGGCGCTGGTGCAGTTGCTGGCCCGGCGCAGCGCACCGAACCGTGAAGTGGCAATCGAGCAGCAGGCCGACCTGCTGGCGGCCTTGGGCAGCCCt > 1:115267/1‑108 (MQ=255) ccGGCGCCGGACCCGGCGCTGGTGCAGTTGCTGGCCCGGCGCAGCGCACCGAACCGTGAAGTGGCAATCGAGCAGCAGGCCGACCTGCTGGCGGCCTTGGGCAGCCCt < 2:115267/108‑1 (MQ=255) cagcgcCGGGTCCGGCGCTGGTGCAGTTGCTGGCCCGGCGCAGCGCACCGAACCGTGAAGTGGCAATCGag < 1:401199/69‑1 (MQ=255) cagcgcCGGGTCCGGCGCTGGTGCAGTTGCTGGCCCGGCGCAGCGCACCGAACCGTGAAGTGGCAATCGag > 2:401199/3‑71 (MQ=255) cGGACCCGGCGCTGGTGCAGTTGCTGGCCCGGCGCAGCGCACCGAACCGTGAAGTGGCAATCGAGCAGCAGGCCGACCTGCTGGCGGCCTTGGGCAGCCCTGAGGTACGGGACGACCGCGAGGTGTTGCTGCACCAGGCGGCGCAGGCCt < 1:400005/150‑1 (MQ=255) gACCCGGCGCTGGTGCAGTTGCTGGCCCGGCGCAGCGCACCGAACCGTGAAGTGGCAATCGAGCAGCAGGCCGACCTGCTGGCGGCCTTGGGCAGCCCTGAGGTACGGGACGACCGCGACCTTCCGCTGCACCAGGCGGCGCAGGCCTAc < 1:323948/150‑1 (MQ=255) cccGGCGCTGGTGCAGTTGCTGGCCCGGCGCAGCGCACCGAACCGTGAAGTGGCAATCGAGCAGCAGGCCGACCTGCTGGCGGCCTTGGGCAGCCCTGAGGTACGGGACGACCGCGAGGTGTTGCTGCACCAGGCGGCGCAGGCCTACGa > 1:395435/1‑150 (MQ=255) | ACGCGTTGGGCGTGCGCCAGTTTCATGTATTGGGTGTGAGCATGGGCGGCATGATTGCCCAGCACCTGGCGGCGATGGCGCCCGAGCGTGTGCGCAGCCTGACGCTGGTCATGTCCAGTTCAGGGGCGGCGGGGCTCCCGGCGCCGGACCCGGCGCTGGTGCAGTTGCTGGCCCGGCGCAGCGCACCGAACCGTGAAGTGGCAATCGAGCAGCAGGCCGACCTGCTGGCGGCCTTGGGCAGCCCTGAGGTACGGGACGACCGCGAGGTGTTGCTGCACCAGGCGGCGCAGGCCTACGA > NC_002947/1219008‑1219305 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |