| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,425,657 | T→C | 18.7% | intergenic (+52/‑453) | PP_1249 → / → PP_1250 | DUF4223 domain‑containing protein/group II intron‑encoding maturase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,425,657 | 0 | T | C | 18.7% | 51.6 / 5.0 | 27 | intergenic (+52/‑453) | PP_1249/PP_1250 | DUF4223 domain‑containing protein/group II intron‑encoding maturase |
| Reads supporting (aligned to +/- strand): ref base T (13/8); new base C (3/2); total (17/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
CTACGACTACCTGTTCCACCCTTCGGTTTCCATCTCCAAGATCATCGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCG‑GGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGC > NC_002947/1425518‑1425801 | cTACGACTACCTGTTCCACCCTTCGGTTTCCATCTCCAAGATCATCGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGCCATg > 2:596065/1‑144 (MQ=255)cTACGACTACCTGTTCCACCCTTCGGTTTCCATCTCCAAGATCATCGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGCCATg < 1:596065/144‑1 (MQ=255) gTTTCCATCTCCAAGATCATCGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCG‑GGGGTTTTTGTGTGCGCCAgg > 1:524967/1‑150 (MQ=255) tttCCATCTCCAAGATCATCGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGGCATGCACATAGCCG‑TGGGTTTTTGTGTGAGCCAGGc > 1:2740/1‑150 (MQ=255) ccAAGAACATCGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCG‑GGGGTTTTTGTGTGCGCCAGGCATGGCGCGt > 2:153923/1‑150 (MQ=255) gCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCG‑GGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAAccc > 1:30107/1‑150 (MQ=255) ccGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCTG‑GGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTg > 2:32136/1‑150 (MQ=255) ccGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCG‑GGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTg < 1:153923/150‑1 (MQ=255) tAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATACAAGGCCCCCGGTCATGCACATGGCCG‑GGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTgcg < 2:329462/123‑1 (MQ=255) tAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATACAAGGCCCCCGGTCATGCACATGGCCG‑GGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTgcg > 1:329462/1‑123 (MQ=255) cTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCG‑GGGGTTTTTGTGTGCGCCAg > 2:74712/1‑100 (MQ=255) cTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCG‑GGGGTTTTTGTGTGCGCCAg < 1:74712/100‑1 (MQ=255) tCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCG‑GGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTg > 1:326956/1‑150 (MQ=255) gcagcagTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCG‑GGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGt > 1:264915/1‑150 (MQ=255) aaTCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCG‑GGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGa < 1:137029/150‑1 (MQ=255) tCTTGGCGGTCCCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGCCAT‑CATCGCGCAGTTGGGTGTATGTGTTCGCCAGGCAGCGCGCGTTGAGCGTTTGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAgg < 1:596565/150‑1 (MQ=17) ttGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCG‑GGGGTTTTtgtgtg < 2:23787/78‑1 (MQ=255) ttGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCG‑GGGGTTTTtgtgtg > 1:23787/1‑78 (MQ=255) ccTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCG‑GGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCctct < 2:524967/150‑1 (MQ=255) tGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCG‑GGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGa < 1:421452/139‑1 (MQ=255) tGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCG‑GGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGa > 2:421452/1‑139 (MQ=255) gTGATCCAACGGCCCCCGTCATGCACATGGACT‑GGGGTTTTTGTGTGCGCCAGGCATGGCACGTTGGGCGTATGGGCGACCCGCTTGGTTACGGTGGCCACGTGGTTAACTTGGAGGGAAATGTGCTCTACACACCCGGCAAggggtacc > 2:134246/1‑146 (MQ=9) tCCAAGGCCCCCGGTCATGCACATGGCCG‑GGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTa > 2:227789/1‑150 (MQ=255) cccccGGTCATGCACATGGCCG‑GGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAg < 2:30107/150‑1 (MQ=255) cccccGGCCATGTGCATGACCG‑GGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAg > 2:109653/1‑150 (MQ=255) ccccGGTCATGCACATGGCCG‑GGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAgg > 2:580177/1‑150 (MQ=255) cccGGCCATGTGCATGACCG‑GGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGGGGTGGTGGCCAAGTGGGTGAGTTGGAGCGGCAATTGCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGc > 1:594192/1‑150 (MQ=11) | CTACGACTACCTGTTCCACCCTTCGGTTTCCATCTCCAAGATCATCGGTGGCTGCGGCCCGATCGATAAACTGCCTCAGCAGCAGTAATCTTGGCGGTACCCTGATCGCGACGCTTGCGGCGTGATCCAAGGCCCCCGGTCATGCACATGGCCG‑GGGGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGC > NC_002947/1425518‑1425801 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |