| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,518,500 | G→A | 13.8% | L55L (CTG→CTA) | murF → | D‑alanyl‑D‑alanine‑adding enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,518,500 | 0 | G | A | 13.8% | 62.1 / 3.0 | 29 | L55L (CTG→CTA) | murF | D‑alanyl‑D‑alanine‑adding enzyme |
| Reads supporting (aligned to +/- strand): ref base G (13/12); new base A (2/2); total (15/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01 | |||||||||||
ACTCAGCCAGCTGACCACAGCACTGAAGGGTCAACACATCGGTGGCGACGCCACGTTCACCGGCGTCAGCATCGATAGCCGCAGTGTCAGCGCCGGGCAACTTTTCGTCGCCCTGGCCGGCCCGCGTTTCGATGGCCATGACTACCTGGCAGATGTGAAAGCCAAAGGCGCGGTAGCCGCCCTGGTCGAGCGTGAAGTAGCCGATGTCGACCTGCCGCAGTTGCAGGTGGCCGATTGCCGCCTGGCCCTCGGCCAGCTCGGTGCATTGAACCGCGCCGCTTTCGACAAGCCAGTG > NC_002947/1518353‑1518647 | aCTCAGCCAGCTGACCACAGCACTGAAGGGTCAACACATCGGTGGCGACGCCACGTTCACCGGCGTCAGCATCGATAGCCGCAGTGTCAGCGCCGGGCAACTTTTCGTCGCCCTGGCCGGCCCGCGTTTCGATGGCCATGACTACCTGGc > 2:268791/1‑150 (MQ=255) agccagcTGACCACAGCACTGAAGGGTCAACACATCGGTGGCGACGCCACGTTCACCGGCGTCAGCATCGATAGCCGCAGTGTCAGCGCCGGGCAACTTTTCGTCGCCCTGGCCGGCCCGCGTTTCGATGGCCATGACTACCTGGCAGAt < 1:268791/150‑1 (MQ=255) agccagcTGACCACAGCACTGAAGGGTCAACACATCGGTGGCGACGCCACGTTCACCGGCGTCAGCATCGATAGCCGCAGTGTCAGCGCCGGGCAACTTTTCGTCGCCCTGGCCGGCCCGCGTTTCGATGGCCATGACTACCTAGCAGAt > 2:179501/1‑150 (MQ=255) acccagcTGACCACAGCACTGAAGGGTCAACACATCGGTGGCGACGCCACGTTCACCGGCGTCAGCATCGATAGCCGCAGTGTCAGCGCCGGGCAACTTTTCGTCGCCCTGGCCGGCCCGCGTTTCGATGGCCATGACTACCTAGCAGAt > 2:179390/3‑150 (MQ=255) cTGAAGGGTCAACACATCGGTGGCGACGCCACGTTCACCGGCGTCAGCATCGATAGCCGCAGTGTCAGCGCCGGGCAACTTTTCGTCGCCCTGGCCGGCCCGCGTTTCGATGGCCATGACTACCTGGCAGATGTGAAAGCCAAAGGcgcg > 1:507834/1‑150 (MQ=255) gAAGGGTCAACACATCGGTGGCGACGCCACGTTCACCGGCGTCAGCATCGATAGCCGCAGTGTCAGCGCCGGGCAACTTTTCGTCGCCCTGGCCGGCCCGCGTTTCGATGGCCATGACTACCTGGCAGATGTGAAAGCCAAAGGCGCGGt < 1:445866/150‑1 (MQ=255) gTCAACACATCGGTGGCGACGCCACGTTCACCGGCGTCAGCATCGATAGCCGCAGTGTCAGCGCCGGGCAACTTTTCGTCGCCCTGGCCGGCCCGCGTTTCGATGGCCATGACTACCTGGCAGATGTGAAAGCCAAAGGCGCGGTAgccg > 2:560315/1‑150 (MQ=255) aaCACATCGGTGGCGACGCCACGTTCACCGGCGTCAGCATCGATAGCCGCAGTGTCAGCGCCGGGCAACTTTTCGTCGCCCTGGCCGGCCCGCGTTTCGATGGCCATGACTACCTGGCAGATGTGAAAGCCAAAGGCGCGGTAGCCGccc < 1:173456/150‑1 (MQ=255) ataggTGGCGCCGCCACGTTCACCGGCGTCAGCATCGATAGCCGCAGTGTCAGCGCCGGGCAACTTTTCGTCGCCCTGGCCGGCCCGCGTTTCGATGGCCATGACTACCTGGCAGATGGGAAAGCCAAAGGCGCGGTAGCCGCCCTGGTc > 2:134070/4‑150 (MQ=255) cGGTGGCGACGCCACGTTCACCGGCGTCAGCATCGATAGCCGCAGTGTCAGCGCCGGGCAACTTTTCGTCGCCCTGGCCGGCCCGCGTTTCGATGGCCATGACTACCTGGCAGATGTGAAAGCCAAAGGCGCGGTAGCCGCCCTGGTCGa < 2:507834/150‑1 (MQ=255) gACGCCACGTTCACCGGCGTCAGCATCGATAGCCGCAGTGTCAGCGCCGGGCAACTTTTCGTCGCCCTGGCCGGCCCGCGTTTCGATGGCCATGACTACCTGGCAGATGTGAAAGCCAAAGGCGCGGTAGCCGCCCTGGTCGAGCGTg > 1:390116/1‑148 (MQ=255) gACGCCACGTTCACCGGCGTCAGCATCGATAGCCGCAGTGTCAGCGCCGGGCAACTTTTCGTCGCCCTGGCCGGCCCGCGTTTCGATGGCCATGACTACCTGGCAGATGTGAAAGCCAAAGGCGCGGTAGCCGCCCTGGTCGAGCGTg < 2:390116/148‑1 (MQ=255) aTAGCCGCAGTGTCAGCGCCGGGCAACTTTTCGTCGCCCTGGCCGGCCCGCGTTTCGATGGCCATGACTACCTGGCAGATGTGAAAGCCAAAGGCGCGGTAGCCGCCCTGGTCGAGCGTGAAGTAGCCGATGTCGACCTGCCGCAGTTGc > 1:34630/1‑150 (MQ=255) cAGTGTCAGCGCCGGGCAACTTTTCGTCGCCCTGGCCGGCCCGCGTTTCGATGGCCATGACTACCTAGCAGATGTGAAAGCCAAAGGCGCGGTAGCCGCCCTGGTCGAGCGTGAAGTAGCCGATGTCGACCTGCCGCAGTTGCAGGTGGc < 1:179390/150‑1 (MQ=255) cAGTGTCAGCGCCGGGCAACTTTTCGTCGCCCTGGCCGGCCCGCGTTTCGATGGCCATGACTACCTAGCAGATGTGAAAGCCAAAGGCGCGGTAGCCGCCCTGGTCGAGCGTGAAGTAGCCGATGTCGACCTGCCGCAGTTGCAGGTGGc < 1:179501/150‑1 (MQ=255) tCAGCGCCGGGCAACTTTTCGTCGCCCTGGCCGGCCCGCGTTTCGATGGCCATGACTACCTGGCAGATGTGAAAGCCAAAGGCGCGGTAGCCGCCCTGGTCGAGCGTg > 1:479390/1‑108 (MQ=255) tCAGCGCCGGGCAACTTTTCGTCGCCCTGGCCGGCCCGCGTTTCGATGGCCATGACTACCTGGCAGATGTGAAAGCCAAAGGCGCGGTAGCCGCCCTGGTCGAGCGTg < 2:479390/108‑1 (MQ=255) tCAGCGCCGGGCAACTTTTCGTCGCCCTGGCCGGCCCGCGTTTCGATGGCCATGACTACCTGGCAGATGTGAAAGCCAAAGGCGCGGTAGCCGCCCTGGTCGAGCGTGAAGTAGCCGATGTCGACCTGCCGCAGTTGCAGGTGGCCGAtt < 2:3477/150‑1 (MQ=255) gtggaCTTTTCGTCGCCCTGGCCGGCCCGCGTTTCGATGGCCATGACTACCTGGCAGATGTGAAAGCCAAAGGCGCGGTAGCCGCCCTGGTCGAGCGTGAAGTAGCCGATGTCGACCTGCCGCAGTTGCAGGTGGCCGATTGCCGCCTgg > 1:21554/5‑150 (MQ=255) ggCAACTTTTCGTCGCCCTGGCCGGCCCGCGTTTCGATGGCCATGACTACCTGGCAGATGTGAAAGCCAAAGGCGCGGTAGCCGCCCTGGTCGAGCGTGAAGTAGCCGATGTCGACCTGCCGCTGTTGTAGGTGGCCGATTGCCGCCTgg < 1:124828/150‑1 (MQ=255) ccTGGCCGGCCCGCGTTTCGATGGCCATGACTACCTGGCAGATGTGAAAGCCAAAGGCGCGGTAGCCGCCCTGGTCGAGCGTGAAGTAGCCGATGTCGACCTGCCGCAGTTGCAGGTGGCCGATTGCCGCCTGGCCCTCGGCCa < 2:568221/144‑1 (MQ=255) ccTGGCCGGCCCGCGTTTCGATGGCCATGACTACCTGGCAGATGTGAAAGCCAAAGGCGCGGTAGCCGCCCTGGTCGAGCGTGAAGTAGCCGATGTCGACCTGCCGCAGTTGCAGGTGGCCGATTGCCGCCTGGCCCTCGGCCa > 1:568221/1‑144 (MQ=255) tGGCCGGCCCGCGTTTCGATGGCCATGACTACCTGGCAGATGTGAAAGCCAAAGGCGCGGTAGCCGCCCTGGTCGAGCGTGAAGTAGCCGATGTCGACCTGCCGCAGTTGCAGGTGGCCGATTGCCGCCTGGCCCTCGGCTAGCTCGGTg < 2:2527/150‑1 (MQ=255) gttCGATGGCCATGACTACCTGGCAGATGTGAGAGCCAAAGGGGCGGTAGCAGCACTGGTGGAGCGTGACGTAGCCGATGTCGACCTGCCGCAGTTGCAGGTGGCCGATTGCCGCCTGGCTCTCGGCACGCTAGGTGCATTGAACGgcgc > 2:59149/2‑150 (MQ=255) aCTACCTGGCAGATGTGAAAGCCAAAGGCGCGGTAGCCGCCCTGGTCGAGCGTGAAGTAGCCGATGTCGACCTGCCGCAGTTGCAGGTGGCCGATTGCCGCCTGGCCCTCGGCCAGCTCGGTGCATTGAACCGCGCCGCTTTCGACAAGc < 1:59149/150‑1 (MQ=255) cTACCTGGCAGATGTGAAAGCCAAAGGCGCGGTAGCCGCCCTGGTCGAGCGTGAAGTAGCCGATGTCGACCTGCCGCAGTTGCAGGTGGCCGATTGCCGCCTGGcc > 2:133594/1‑106 (MQ=255) cTACCTGGCAGATGTGAAAGCCAAAGGCGCGGTAGCCGCCCTGGTCGAGCGTGAAGTAGCCGATGTCGACCTGCCGCAGTTGCAGGTGGCCGATTGCCGCCTGGcc < 1:133594/106‑1 (MQ=255) tACCTGGCAGATGTGAAAGCCAAAGGCGCGGTAGCCGCCCTGGTCGAGCGTGAAGTAGCCGATGTCGACCTGCCGCAGTTGCAGGTGGCCGATTGCCGCCTGGCCCTCGGCCAGCTCGGTGCATTGAACCGCGCCGCTTTCGACAAGCCa > 1:536780/1‑150 (MQ=255) cTGGCAGATGTGAAAGCCAAAGGCGCGGTAGCCGCCCTGGTCGAGCGTGAAGTAGCCGATGTCGACCTGCCGCAGTTGCAGGTGGCCGATTGCCGCCTGGCCCTCGGCCAGCTCGGTGCATTGAACCGCGCCGCTTTCGACAAGCCAgtg > 2:364007/1‑150 (MQ=255) | ACTCAGCCAGCTGACCACAGCACTGAAGGGTCAACACATCGGTGGCGACGCCACGTTCACCGGCGTCAGCATCGATAGCCGCAGTGTCAGCGCCGGGCAACTTTTCGTCGCCCTGGCCGGCCCGCGTTTCGATGGCCATGACTACCTGGCAGATGTGAAAGCCAAAGGCGCGGTAGCCGCCCTGGTCGAGCGTGAAGTAGCCGATGTCGACCTGCCGCAGTTGCAGGTGGCCGATTGCCGCCTGGCCCTCGGCCAGCTCGGTGCATTGAACCGCGCCGCTTTCGACAAGCCAGTG > NC_002947/1518353‑1518647 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |