Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,327,1270CT38.9% 27.3 / 35.6 37intergenic (+97/‑196)PP_16SE/PP_23SE16S ribosomal RNA/23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (11/11);  new base T (7/7);  total (19/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAG  >  NC_002947/1326986‑1327231
                                                                                                                                             |                                                                                                        
tAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATAAAATATTTAATAGATGATATAAGCCTCCTGATGATCGCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATg                                                                                                  >  1:11745/1‑150 (MQ=11)
  aCAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGtt                                                                                                >  1:515821/1‑150 (MQ=32)
    aaGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGa                                                                                              <  2:325586/150‑1 (MQ=32)
    aaGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGa                                                                                              >  1:255011/1‑150 (MQ=32)
            cGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGc                                                                                      <  2:405378/150‑1 (MQ=32)
               atgggAACCTGCGGCTGGATCACCTCCTTAATCGACGGCATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCCTTGTCGAAGAGGTTTTTCCTGGNCAGNGCNTGGAAATGAGCANTCGCATCGAATGTTGATTTCTGGCttt                                                                                   <  1:584713/148‑1 (MQ=11)
                      ccTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTc                                                                               >  2:79726/1‑147 (MQ=17)
                      ccTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTc                                                                               <  1:79726/147‑1 (MQ=17)
                        tGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCATATc                                                                          <  2:310255/150‑1 (MQ=17)
                              tGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCtt                                                                    >  1:111293/1‑150 (MQ=32)
                                 aTCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTaa                                                                 >  1:573222/1‑150 (MQ=32)
                                      ctcctTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAAtt                                                            >  2:572261/1‑150 (MQ=32)
                                       tcctTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTc                                                           <  1:572261/150‑1 (MQ=32)
                                         ctTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTAt                                                                                                        <  2:363637/103‑1 (MQ=18)
                                         ctTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCgg                                                         >  2:283636/1‑150 (MQ=17)
                                         ctTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCgg                                                         >  1:308982/1‑150 (MQ=17)
                                            aaTCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGata                                                      <  1:283636/150‑1 (MQ=17)
                                              tCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATAtg                                                    >  1:550767/1‑150 (MQ=17)
                                              tCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATAtg                                                    >  2:258272/1‑150 (MQ=32)
                                                          gCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAg                                        <  2:515821/150‑1 (MQ=32)
                                                           ccTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGa                                       <  2:255011/150‑1 (MQ=32)
                                                             tgctgATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACt                                     >  1:141061/1‑150 (MQ=17)
                                                             tgctgATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACt                                     <  1:616780/150‑1 (MQ=17)
                                                                       cTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCac                                                                                                        <  2:127060/73‑2 (MQ=25)
                                                                       cTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCac                                                                                                        >  1:127060/1‑72 (MQ=25)
                                                                       cTCCCACACGAATTGCTTGATTCATTGTCGAAGACGATTTTCATGGTCACAACCTGGAACTGCGCATTGGGATCGAATGGTTATTTCTGGCTTTTGGCATATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGt                           >  2:70241/1‑150 (MQ=11)
                                                                          ccACACGCATTGCTTGATTACTTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTc                        >  2:6716/1‑150 (MQ=12)
                                                                          ccACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTc                        >  1:539042/1‑150 (MQ=17)
                                                                               cGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCActgc                   <  2:308982/150‑1 (MQ=17)
                                                                                 aaTTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCActgctg                 >  2:274466/1‑150 (MQ=32)
                                                                                  aTTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATATACTGAACACCAGTTTCACTGCtgg                <  2:11745/150‑1 (MQ=17)
                                                                                   ttGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCtggt               <  2:573222/150‑1 (MQ=32)
                                                                                       ttGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGAt           >  2:173261/1‑150 (MQ=32)
                                                                                               ttGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTgatagata                                           <  2:210214/110‑1 (MQ=18)
                                                                                               ttGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTgatagata                                           >  1:210214/1‑110 (MQ=18)
                                                                                                tGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAg  <  2:341674/150‑1 (MQ=17)
                                                                                                tGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAg  <  2:111293/150‑1 (MQ=32)
                                                                                                                                             |                                                                                                        
TAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAG  >  NC_002947/1326986‑1327231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑