Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 924,322 Δ1 bp 7.9% coding (874/2169 nt) PP_0804 ← protein secretion ABC transporter permease/ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947924,3220T. 7.9% 367.6 / 26.0 127coding (874/2169 nt)PP_0804protein secretion ABC transporter permease/ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (61/53);  new base . (5/5);  total (68/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.25e-01

Only 100 of 127 total aligned reads displayed.

TTGGCCAACCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGCGCTT  >  NC_002947/924174‑924469
                                                                                                                                                    |                                                                                                                                                     
  ggCCAACCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTcg‑c                                                                                                                                                   <  1:507429/150‑1 (MQ=255)
    ccAACCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑Cgg                                                                                                                                                 <  1:1222619/150‑1 (MQ=255)
     cAACCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGAAGGTCGGCCAGCAATGGCG‑CGGt                                                                                                                                                >  1:1179464/1‑150 (MQ=255)
      aaCCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑g                                                                                                                                              >  1:1871928/1‑150 (MQ=255)
         cGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTg                                                                                                                                           <  1:1153892/150‑1 (MQ=255)
           tGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGa                                                                                                                                         >  2:2216873/1‑150 (MQ=255)
           ggCCAGGCGCCTTTGTGCAAGCACCCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCCGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGTCCAGCACCG‑CGACGGT‑GCTggc                                                                                                                                         <  2:2345376/149‑2 (MQ=255)
                ggCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGa                                                                                                                                    <  1:440144/150‑1 (MQ=255)
                   gcCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGCACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCa                                                                                                                                 <  2:2403087/150‑1 (MQ=255)
                        tgtgCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTcg                                                                                                                            <  2:465230/150‑1 (MQ=255)
                          tgCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTcgcg                                                                                                                          >  2:463487/1‑150 (MQ=255)
                           gcaagcaaCCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTcg‑cg                                                                                                                                                  >  1:2407585/1‑126 (MQ=255)
                           gcaagcaaCCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑AGGT‑GCTGGACGTGATCAGGTCGCgg                                                                                                                         >  1:698275/1‑150 (MQ=255)
                            caagcaaCCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTGCGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCACGTCGGCAAGCACCGTCG‑TGGT‑GCTGGACGTGATCAGGTCGCggc                                                                                                                        >  2:795425/1‑149 (MQ=255)
                            caagcaaCCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGa                                                                                                                        <  1:65793/150‑1 (MQ=255)
                            caagcaaCCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCG‑CGACGGT‑GCTgg                                                                                                                                          >  2:717603/1‑132 (MQ=255)
                            caagcaaCCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCG‑CGACGGT‑GCTgg                                                                                                                                          <  1:717603/132‑1 (MQ=255)
                             aagcaaCCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGAt                                                                                                                       >  2:1578455/1‑150 (MQ=255)
                              agcaaCCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCG‑CGACGGT‑GCTggc                                                                                                                                         <  1:131300/131‑2 (MQ=255)
                               gcaaCCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGAtag                                                                                                                     >  1:687921/1‑148 (MQ=255)
                               gcaaCCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGACAGGACCCTCT‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCg                                                                                                                     >  2:564489/1‑150 (MQ=255)
                                  aCCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGa                                                                                                                                         >  1:1063322/1‑127 (MQ=255)
                                  aCCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGa                                                                                                                                         <  2:1063322/127‑1 (MQ=255)
                                  aCCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATc                                                                                                                      <  1:612183/146‑1 (MQ=255)
                                  aCCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATc                                                                                                                      >  2:612183/1‑146 (MQ=255)
                                   accGGGTAGCACAATGGACAGCAGCGCGACCAGAGGGATGAACACCAGTACGCGGCCGACCAGCCAGAACACGAACAGGAACTGGAGTAAGAACGGCAGGTCGGCCAGCACTGTCG‑CGGT‑GATGGACGTGATCAGGTCGCGGGTCGACTc                                                                                                                 >  2:1036370/2‑150 (MQ=255)
                                          agcaCCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGCGA‑CGGT‑GCTgg                                                                                                                                          >  2:775476/1‑118 (MQ=255)
                                              ccATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCa                                                                                                      >  2:2130939/1‑150 (MQ=255)
                                                   gCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGAACCG‑GG‑CTGTAGGTTGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGc                                                                                                 >  2:1638396/1‑150 (MQ=255)
                                                    ccAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTcgc                                                                                                                           <  2:653546/123‑1 (MQ=255)
                                                    ccAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCg                                                                                                >  2:1742523/1‑150 (MQ=255)
                                                     cagcagCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAGCACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCga                                                                                               <  1:1036370/150‑1 (MQ=255)
                                                     cagcagCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCga                                                                                               >  1:1041944/1‑150 (MQ=255)
                                                     cagcagCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCga                                                                                               >  2:374821/1‑150 (MQ=255)
                                                         agcGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCCGCTCGCGCAACTGCGAGATg                                                                                           >  1:827829/1‑150 (MQ=255)
                                                            gcgACCAGCGGGATGAACACTAGTACGCCGCCGATCACCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAAc                                                                                        <  1:795425/150‑1 (MQ=255)
                                                            gcgACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAAc                                                                                        <  1:349043/150‑1 (MQ=255)
                                                            gcgACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCAAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAAc                                                                                        <  1:21267/150‑1 (MQ=255)
                                                                    cGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCgg                                                                                                                         <  2:189206/109‑1 (MQ=255)
                                                                    cGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCgg                                                                                                                         >  1:189206/1‑109 (MQ=255)
                                                                    cGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGt                                                                                <  2:1753466/150‑1 (MQ=255)
                                                                     gggATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGTAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTg                                                                               <  2:1245614/150‑1 (MQ=255)
                                                                       gATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGa                                                                             <  2:1485824/150‑1 (MQ=255)
                                                                         tGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACt                                                                           >  1:1625833/1‑150 (MQ=255)
                                                                           aaCACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCCGCGCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTg                                                                         >  1:500725/1‑150 (MQ=255)
                                                                           aaCACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTg                                                                         <  1:791795/150‑1 (MQ=255)
                                                                            acacCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTgg                                                                        >  1:318748/1‑150 (MQ=255)
                                                                            acacCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTgg                                                                        <  1:463487/150‑1 (MQ=255)
                                                                               ccAGTACGCCGCCGATCAGCCAGAACACGAAAAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACAGCGAGATGAACGAGCCGGTGGACTTGGGcc                                                                     <  2:236572/150‑1 (MQ=255)
                                                                                cAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCa                                                                                                                                 >  1:2445556/1‑89 (MQ=255)
                                                                                cAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCa                                                                                                                                 <  2:2445556/89‑1 (MQ=255)
                                                                                cAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCg                                                                                                >  2:1229152/1‑122 (MQ=255)
                                                                                 aGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGc                                                                   >  2:1483303/1‑150 (MQ=255)
                                                                                 aGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCACAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGc                                                                   >  1:1438748/1‑150 (MQ=255)
                                                                                      gccgccGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTc                                                                                                           >  2:1596387/1‑105 (MQ=255)
                                                                                        cgccgATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGAc                                                                                                                                        >  2:1272826/1‑74 (MQ=255)
                                                                                        cgccgATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGAc                                                                                                                                        <  1:1272826/74‑1 (MQ=255)
                                                                                         gccgATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGtt                                                           >  2:1138051/1‑150 (MQ=255)
                                                                                           cgATCAGCCAGAACACGAACAGGAACACGAGGTAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTgc                                                         <  2:301509/150‑1 (MQ=255)
                                                                                            gATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGaa                                                                                         >  1:358933/1‑117 (MQ=255)
                                                                                            gATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGaa                                                                                         <  2:358933/117‑1 (MQ=255)
                                                                                             aTCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTgcgc                                                       <  1:2130939/150‑1 (MQ=255)
                                                                                                    aGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCGGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTgcgcaggcgca                                                >  1:1727022/1‑150 (MQ=255)
                                                                                                    aGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTgcgcaggcgca                                                >  1:1548399/1‑150 (MQ=255)
                                                                                                     gAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCCGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTgcgcaggcgcag                                               <  2:39187/150‑1 (MQ=255)
                                                                                                     gAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTgcgcaggcgcag                                               <  1:1884400/150‑1 (MQ=255)
                                                                                                      aaCACGACCAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCCGGGTGG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGAAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGcgcaggcgcagg                                              <  1:1321085/150‑1 (MQ=255)
                                                                                                      aaCACGAACAGGAACAGCAGGAAGAACGGAAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGcgcaggcgcagg                                              >  2:441754/1‑150 (MQ=255)
                                                                                                         acGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCAcc                                                                <  2:1833529/129‑1 (MQ=255)
                                                                                                             acaggaacagCAGGAAGAACGGCAGGTCGGCCAGAATCTTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGc                                       >  1:564862/1‑150 (MQ=255)
                                                                                                                 gaacagCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGCCTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTa                                   <  2:1683686/150‑1 (MQ=255)
                                                                                                                 gaacagCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTa                                   >  1:1590775/1‑150 (MQ=255)
                                                                                                                 gaacagCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGACTTGCGCAGGCGCAGGGCATGGCCGTa                                   <  1:1012742/150‑1 (MQ=255)
                                                                                                                   acagCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATg                                                                                           <  2:489134/92‑1 (MQ=255)
                                                                                                                       cagGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAg                             >  2:1214100/1‑150 (MQ=255)
                                                                                                                        agGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAgg                            <  1:1578455/150‑1 (MQ=255)
                                                                                                                        agGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAgg                            >  2:1588856/1‑150 (MQ=255)
                                                                                                                         ggAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCg                                     <  2:469546/140‑1 (MQ=255)
                                                                                                                         ggAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCg                                     >  1:469546/1‑140 (MQ=255)
                                                                                                                          gaagaaCGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAAc                                                                                        >  2:195691/1‑88 (MQ=255)
                                                                                                                          gaagaaCGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAAc                                                                                        <  1:195691/88‑1 (MQ=255)
                                                                                                                          gaagaaCGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTc                          <  1:2216873/150‑1 (MQ=255)
                                                                                                                                 ggCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTggg                                                                       >  2:1065804/1‑98 (MQ=255)
                                                                                                                                     ggTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCa               >  1:2073417/1‑150 (MQ=255)
                                                                                                                                     ggTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATACGGTGCAGGAGGGTCAAGTGTACTCACAACTGCTAGTTGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGAGCAGGTCGCTCACCCGCa               >  1:46025/1‑150 (MQ=255)
                                                                                                                                      gTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCga                                                                                               >  2:1795174/1‑69 (MQ=255)
                                                                                                                                      gTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCga                                                                                               <  1:1795174/69‑1 (MQ=255)
                                                                                                                                      gTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAg              <  2:318748/150‑1 (MQ=255)
                                                                                                                                      gTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAg              >  1:2353285/1‑150 (MQ=255)
                                                                                                                                      gTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGCGCATGGCCGTAGACCAGGTCGCTCACCCGCAg              <  2:1349183/150‑1 (MQ=255)
                                                                                                                                      cacgtccAGCACCG‑CGACGGT‑GCTGGCCGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAg              >  2:1570549/6‑150 (MQ=255)
                                                                                                                                         gtccAGCACCG‑CGACGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGtt                                                           >  2:297502/3‑102 (MQ=255)
                                                                                                                                         gtccAGCACCG‑CGACGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGtt                                                           <  1:297502/100‑1 (MQ=255)
                                                                                                                                          gCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGCAACATCCTTTCTCAACTTCTAGATGAACGAGCAGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCg          <  1:1742523/150‑1 (MQ=255)
                                                                                                                                             aGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGgcg       >  2:1657675/1‑150 (MQ=255)
                                                                                                                                             aGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGgcg       >  2:1657629/1‑150 (MQ=255)
                                                                                                                                              gCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGCGCCGGGGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGgcgc      <  1:287877/150‑1 (MQ=255)
                                                                                                                                               cACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGgcgcg     >  1:1175924/1‑150 (MQ=255)
                                                                                                                                                aCCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAgg                            >  1:412289/1‑126 (MQ=255)
                                                                                                                                                  cGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGCGCtt  >  1:28755/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TTGGCCAACCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCG‑CGGT‑GCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGCGCTT  >  NC_002947/924174‑924469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑