Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,232,743	Δ1 bp	6.8%	intergenic (‑25/+192)	glpD ← / ← glpR	aerobic glycerol‑3‑phosphate dehydrogenase/DNA‑binding transcriptional repressor GlpR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,232,743	0	A	.	6.8%	216.0 / 10.2	74	intergenic (‑25/+192)	glpD/glpR	aerobic glycerol‑3‑phosphate dehydrogenase/DNA‑binding transcriptional repressor GlpR
Reads supporting (aligned to +/- strand):  ref base A (30/37);  new base . (3/2);  total (34/40)
Fisher's exact test for biased strand distribution p-value = 6.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.11e-01

CCTTCAAGCCGCGACCAGCGGCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGCTTGGGTGAGGGGGCGCT  >  NC_002947/1232595‑1232888                                                                                                                                                      |                                                                                                                                                  ccTTCAAGCCGCGACCAGCGGCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGaa                                                                                                                                                  >  2:1372428/1‑150 (MQ=255)  cTTCAAGCCGCGACCAGCGGCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAAt                                                                                                                                                 >  2:1011704/1‑150 (MQ=255)    tCAAGCCGCGACCAGCGGCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAAtgt                                                                                                                                               >  2:999596/1‑150 (MQ=255)     cAAGCCGCGACCAGCGGCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAAtgtg                                                                                                                                              >  1:805532/1‑150 (MQ=255)     cAAGCCGCGACCAGCGGCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAAtgtg                                                                                                                                              <  1:1258274/150‑1 (MQ=255)       aGCCGCGACCAGCGGCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGaa                                                                                                                                            <  1:2434953/150‑1 (MQ=255)       aGCCGCGACCAGCGGCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGCACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGaa                                                                                                                                            <  1:1011704/150‑1 (MQ=255)             gACCAGCGGCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGa                                                                                                                                      <  1:2083876/150‑1 (MQ=255)              aCCAGCGGCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGAt                                                                                                                                     <  2:821561/150‑1 (MQ=255)               ccAGCGGCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACATTCG‑ATGTGAACa                                                                                                                                          >  2:1178847/1‑144 (MQ=255)               ccAGCGGCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACATTCG‑ATGTGAACa                                                                                                                                          <  1:1178847/144‑1 (MQ=255)                 aGCGGCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCCAGGTCATAGCAGTTGGCTGTAGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGtt                                                                                                                                  >  1:685161/1‑150 (MQ=255)                  gcggcgTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTc                                                                                                                                 <  2:2122720/150‑1 (MQ=255)                  gcggcgTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTc                                                                                                                                 <  2:199994/150‑1 (MQ=255)                  gcggcgTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTc                                                                                                                                 <  1:228290/150‑1 (MQ=255)                    ggcgTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGTGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGt                                                                                                                               <  1:2017230/150‑1 (MQ=255)                     gcgTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGtt                                                                                                                              >  1:956452/1‑150 (MQ=255)                           gCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGaa                                                                                                                        <  2:129551/150‑1 (MQ=255)                             ggCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGaaaa                                                                                                                      <  2:1411910/149‑1 (MQ=255)                                gATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAatat                                                                                                                   >  1:869838/1‑150 (MQ=255)                                  tGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCCTAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGACGTTCGTTTGCGAAAATATTa                                                                                                                 <  1:1206215/150‑1 (MQ=255)                                  tGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTa                                                                                                                 <  2:805532/150‑1 (MQ=255)                                      cACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAgcgc                                                                                                             >  1:1789844/1‑150 (MQ=255)                                      cACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGATGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TC‑AATGTTCACAttcgatg                                                                                                                                   >  1:1548477/1‑122 (MQ=255)                                            aTTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGa                                                                                                       >  1:1188287/1‑150 (MQ=255)                                            aTTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGa                                                                                                       >  2:590995/1‑150 (MQ=255)                                                       cgccgATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGc                                                                                            >  2:2020857/1‑150 (MQ=255)                                                           gATTACGGCAAGGTCATAGCAGTTAAAAGACTGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCcagt                                                                                        >  1:1242625/1‑150 (MQ=255)                                                            aTTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCcagtc                                                                                       <  2:1188287/150‑1 (MQ=255)                                                             ttACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCcagtca                                                                                      >  2:2069443/1‑150 (MQ=255)                                                               aCGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCGTCTTGAGGCTGTTCCCA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCcagtcagt                                                                                    <  1:1354926/150‑1 (MQ=255)                                                                 ggCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGCAAATATGAGCGCAACAGAACGCCGGTCGCCAATCAGtct                                                                                  >  2:787592/1‑150 (MQ=255)                                                                      ggTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCtata                                                                             <  1:6109/150‑1 (MQ=255)                                                                           tAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAAAGCCTGTCGCCAGTCAGTCTCTATAGaaaa                                                                        <  1:2277989/150‑1 (MQ=255)                                                                             gCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCcagt                                                                                        <  2:1394616/132‑1 (MQ=255)                                                                             gCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCcagt                                                                                        >  1:1394616/1‑132 (MQ=255)                                                                             gCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGaaaaaa                                                                      >  1:710106/1‑150 (MQ=255)                                                                              cAGTTGGGTGTTGGGGGCTAGGACGAAACAGGCTGGGAAATGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAATATTGATGTTCGTTTGCGAAAATATTAGAGCGACAGGACGCGGGTCGCCAGTCAGTCGCTATATaatgaac                                                                     <  2:972870/150‑6 (MQ=255)                                                                              cAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAAc                                                                     >  2:976429/1‑150 (MQ=255)                                                                                  tGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGAt                                                                 <  1:410943/150‑1 (MQ=255)                                                                                      tGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCaaa                                                             <  1:735518/150‑1 (MQ=255)                                                                                        ttGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAAtgt                                                                                                                                               <  2:203143/66‑1 (MQ=255)                                                                                        ttGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAAtgt                                                                                                                                               >  1:203143/1‑66 (MQ=255)                                                                                           ggggCTGGGACGAAAATGGCTGGGACACGGGCAAGGCCTCCTGGCGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCCGCCAGTCTCTATAGAACAAACGAATAAAACGACa                                                        >  2:290304/1‑150 (MQ=255)                                                                                            gggCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACaa                                                       <  1:999596/150‑1 (MQ=255)                                                                                                     cGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGaa                                              <  1:714811/150‑1 (MQ=255)                                                                                                      gAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGa                                                                           >  2:2204662/1‑120 (MQ=255)                                                                                                      gAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGa                                                                           <  1:2204662/120‑1 (MQ=255)                                                                                                      gAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGa                                                                  >  1:114844/1‑129 (MQ=255)                                                                                                      gAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGa                                                                  <  2:114844/129‑1 (MQ=255)                                                                                                      gAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGAGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAAc                                             <  2:742716/150‑1 (MQ=255)                                                                                                        aaCTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACAt                                           >  2:40855/1‑150 (MQ=255)                                                                                                            ggctggGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTAc                                       <  1:1019886/150‑1 (MQ=255)                                                                                                                 ggACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGaaaa                                  <  2:710106/150‑1 (MQ=255)                                                                                                                        ggCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAggg                           <  1:1372428/150‑1 (MQ=255)                                                                                                                           aaGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGt                        >  2:110590/1‑150 (MQ=255)                                                                                                                            aGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTa                       <  1:590995/150‑1 (MQ=255)                                                                                                                             ggCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCTAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTAc                      <  2:685161/150‑1 (MQ=255)                                                                                                                              gCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGaa                                              <  1:41098/125‑1 (MQ=255)                                                                                                                              gCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGaa                                              >  2:41098/1‑125 (MQ=255)                                                                                                                               cctcctGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACgg                    >  2:2112788/1‑150 (MQ=255)                                                                                                                                  cctGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGCtt                 <  2:1789844/150‑1 (MQ=255)                                                                                                                                       ggCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGCTTGGGTg            >  1:303986/1‑150 (MQ=255)                                                                                                                                       caatGTTCACATTCG‑ATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGCTTgg               >  1:1957186/4‑147 (MQ=255)                                                                                                                                       caatGTTCACA‑TTCGATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGCTTgg               <  2:1957186/144‑1 (MQ=255)                                                                                                                                        gCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGCTTGGGTGa           <  1:2020857/150‑1 (MQ=255)                                                                                                                                         atGTTCACA‑TTCGATGTGAACATTCATGTTCGTTTGCTAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCACATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGCTTGGGTGAg          >  1:2017952/2‑150 (MQ=255)                                                                                                                                          tGTTCACATTCG‑ATGCGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCta                                                                               >  2:378010/1‑80 (MQ=255)                                                                                                                                          tGTTCACATTCG‑ATGCGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCta                                                                               <  1:378010/80‑1 (MQ=255)                                                                                                                                          tGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGCTTGGGTGAgg         <  1:40855/150‑1 (MQ=255)                                                                                                                                           gTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGCTTGGGTGAggg        >  2:1670462/1‑150 (MQ=255)                                                                                                                                              caca‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGCTTGGGTGAGGGGgc     <  1:341383/150‑1 (MQ=255)                                                                                                                                               aca‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGCTTGGGTGAGGGGgcg    >  1:1194524/1‑150 (MQ=255)                                                                                                                                                ca‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGCTTGGGTGAGGGGgcgc   >  1:1867443/1‑150 (MQ=255)                                                                                                                                                 a‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGCTTGGGTGAGGGGGCGCt  >  1:914226/1‑150 (MQ=255)                                                                                                                                                      |                                                                                                                                                  CCTTCAAGCCGCGACCAGCGGCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACA‑TCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGCTTGGGTGAGGGGGCGCT  >  NC_002947/1232595‑1232888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑