| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,468,121:1 | +A | 11.5% | coding (819/1047 nt) | mltB ← | membrane‑bound lytic murein transglycosylase B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,468,121 | 1 | . | A | 11.5% | 294.7 / 37.8 | 87 | coding (819/1047 nt) | mltB | membrane‑bound lytic murein transglycosylase B |
| Reads supporting (aligned to +/- strand): ref base . (40/37); new base A (7/3); total (47/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.97e-01 | |||||||||||
AGTGATCGCGTAGAAGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAGATATTGATATG > NC_002947/5467974‑5468270 | aGTGATCGCGTAGAAGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑Tc < 1:786885/150‑1 (MQ=255) tGATCGCGTAGAAGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGa > 1:95974/1‑150 (MQ=255) gATCGCGTAGAAGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCGGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGAt > 1:254311/1‑150 (MQ=255) gTAGAAGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCggt < 1:1756709/150‑1 (MQ=255) tAGAAGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCggtg < 2:1631483/150‑1 (MQ=255) tAGAAGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCggtg > 2:2288370/1‑150 (MQ=255) aaGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTc > 1:220314/1‑150 (MQ=255) aGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGCATCGA‑GCCggtgaaaa < 1:1445819/150‑5 (MQ=255) gTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAg < 1:1908186/150‑1 (MQ=255) gTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAg < 1:1889010/150‑1 (MQ=255) gTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTCCACCGGCATCGA‑GCCggtgaaaac < 2:1290989/150‑6 (MQ=255) gTTCTTCAGGCCCATCCAGTACGCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCACTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAg < 2:1099774/150‑1 (MQ=255) tcttcAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGc > 2:214195/1‑150 (MQ=255) tcttcAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGc < 1:214195/150‑1 (MQ=255) gCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTtcgtc > 2:441990/1‑150 (MQ=255) gCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTtcgtc > 1:825547/1‑150 (MQ=255) cATCCACTTCTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGgc < 1:1606188/150‑1 (MQ=255) aTCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGgcg < 2:332869/150‑1 (MQ=255) tCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAg > 2:586329/1‑136 (MQ=255) tCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAg < 1:586329/136‑1 (MQ=255) ccAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGgcgcg > 2:1763108/1‑150 (MQ=255) aGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCt > 1:2405545/1‑150 (MQ=255) tCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCt > 1:2110240/1‑150 (MQ=255) ccGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCAcc > 1:516375/1‑150 (MQ=255) tctcGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGATGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAgg > 1:124234/1‑150 (MQ=255) ccTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGAc > 1:1674090/1‑150 (MQ=255) cTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCACCGAATCATGGACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACt > 1:2256184/1‑150 (MQ=255) tCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTg > 1:434414/1‑150 (MQ=255) cGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGa < 1:1195625/150‑1 (MQ=255) gagaCGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGAc < 2:2405545/150‑1 (MQ=255) gaCGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACca < 1:2436478/150‑1 (MQ=255) gTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCg < 2:1109299/150‑1 (MQ=255) tAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCgg > 1:2013986/1‑150 (MQ=255) gCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCCACTCACCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCCCTCCCCTTCCACCCAGGCGCGACTGACCCCACCCTCGCCGGCCAccc > 2:462696/1‑150 (MQ=255) atcatcGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGcc > 2:1283687/1‑150 (MQ=255) tcatcGCGCGGCGAATCATGCACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTAACCGGC‑TCGCTGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACGGACCACACCCTCGCCGGCCACCCAGCCg > 1:584187/1‑150 (MQ=255) gcgcAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGgcg > 1:259726/1‑150 (MQ=255) aGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTg > 1:2078563/1‑150 (MQ=255) gCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCCCCCAGGCGCGACTGACCacac < 2:931073/120‑1 (MQ=255) gCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCacac > 1:931073/1‑120 (MQ=255) aTCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTa < 2:1148580/150‑1 (MQ=255) tCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGGCTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAg < 2:254311/150‑1 (MQ=255) aCCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGcc > 1:1674746/1‑150 (MQ=255) cAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAg > 1:1522180/1‑135 (MQ=255) cAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAg < 2:1522180/135‑1 (MQ=255) cAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTg > 2:1580480/1‑150 (MQ=255) ccAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCTCTTGAAGTAGCTGGCCACGCTGCCg > 1:1818267/1‑150 (MQ=255) ccAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTAAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTGGCTGGCCACGCTGCCg > 1:507245/1‑150 (MQ=255) aaGCGTACGCAACTCCCCTACCGTTTTCGCCGGC‑TCGATGCCGGTGGTCAGGCCGTCGCCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGAt < 2:1876719/150‑1 (MQ=255) aaGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TGGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGAt < 2:879451/150‑1 (MQ=255) aaGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGAt < 1:441990/150‑1 (MQ=255) aGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATg < 2:825547/150‑1 (MQ=255) aGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATg < 2:1674090/150‑1 (MQ=255) gCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATgg > 2:2415027/1‑150 (MQ=255) cGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCt < 2:2302298/141‑1 (MQ=255) cGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCt > 1:2302298/1‑141 (MQ=255) aCGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATc < 2:1674746/150‑1 (MQ=255) gCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGt > 1:1393950/1‑150 (MQ=255) aCTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTgg > 2:810384/1‑126 (MQ=255) aCTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTgg < 1:810384/126‑1 (MQ=255) aCTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAg < 1:1763108/150‑1 (MQ=255) cTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTTGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAgg < 1:2443515/150‑1 (MQ=255) tCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGc > 1:1521462/1‑133 (MQ=255) tCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGc < 2:1521462/133‑1 (MQ=255) tCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAggg > 1:1617352/1‑150 (MQ=255) ccTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCa < 1:2169605/61‑1 (MQ=255) ccTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCa > 2:2169605/1‑61 (MQ=255) cTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGgttgt > 1:2418566/1‑150 (MQ=255) tACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCATCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGgttgtt > 2:674773/1‑150 (MQ=255) ccGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAg > 1:1701231/1‑110 (MQ=255) ccGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAg < 2:1701231/110‑1 (MQ=255) cGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCa < 1:2415027/150‑1 (MQ=255) gTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAg > 2:9443/1‑139 (MQ=255) gTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAg < 1:9443/139‑1 (MQ=255) gTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAg > 2:2376552/1‑150 (MQ=255) ctgaccACCGGCATCGA‑GCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAg > 2:2121389/6‑150 (MQ=255) ctgaccACCGGCATCGA‑GCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAg > 1:1176649/6‑150 (MQ=255) gaccACCGGCATCGA‑GCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCacac < 1:888854/71‑1 (MQ=38) gaccACCGGCATCGA‑GCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCacac > 2:888854/4‑74 (MQ=38) cACCGGCATCGA‑GCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAGATAtt > 2:944429/1‑150 (MQ=255) cACCGGCATCGA‑GCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAGATAtt > 2:2279596/1‑150 (MQ=255) cACCGGCATCGA‑GCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAGATAtt > 2:1630352/1‑150 (MQ=255) cACCGGCATCGA‑GCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAGATAtt > 2:1026052/1‑150 (MQ=255) ccGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAGATATTGa < 2:2418566/150‑1 (MQ=255) cGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAGATATTGat < 1:1580480/150‑1 (MQ=255) cGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAGATATTGat < 2:2110240/150‑1 (MQ=255) cGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAGATATTGat < 2:124234/150‑1 (MQ=255) c‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAGATATTGATATg < 1:2288370/150‑1 (MQ=255) | AGTGATCGCGTAGAAGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAGATATTGATATG > NC_002947/5467974‑5468270 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |