Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,948,729:1 +G 5.0% intergenic (‑54/+28) PP_mr62 ← / ← trxA Pseudomon‑Rho regulatory region/thioredoxin I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,948,7291.G 5.0% 409.4 / 12.4 99intergenic (‑54/+28)PP_mr62/trxAPseudomon‑Rho regulatory region/thioredoxin I
Reads supporting (aligned to +/- strand):  ref base . (46/48);  new base G (2/3);  total (48/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

Only 100 of 107 total aligned reads displayed.

GGCCGCGCCGCTGGATGGCGACAGGATCGCGTACTGATTCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTCTTGAACAGCATCAGCGTCGGGATACCACGCACACCGTGC  >  NC_002947/5948584‑5948874
                                                                                                                                                  |                                                                                                                                                 
ggCCGCGCCGCTGGATGGCGACAGGATCGCGTACTGATTCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑Gccc                                                                                                                                               >  2:2032326/1‑150 (MQ=255)
 gccgcgccgcTGGATGGCGACAGGATCGCGTACTGATTCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑Gcccc                                                                                                                                              <  1:617508/150‑1 (MQ=255)
      gccgcTGGATGGCGACAGGATCGCGTACTGATTCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGGCCCCGCaa                                                                                                                                          >  1:1868481/1‑149 (MQ=255)
          cgggATGGCGACAGGATCGCGTACTGATTCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCg                                                                                                                                     <  2:169211/148‑1 (MQ=255)
          cTGGATGGCGACAGGATCGCGTACTGATTCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCg                                                                                                                                     >  2:353373/1‑150 (MQ=255)
            ggATGGCGACAGGATCGCGTACTGATTCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCggg                                                                                                                                   <  1:358406/150‑1 (MQ=255)
                 gCGACAGGATCGCGTACTGATTCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGGCCCCGCAAATGCGGGGctt                                                                                                                               <  2:1868481/150‑3 (MQ=255)
                   gACAGGATCGCGTACTGATTCGAATTAGGGATGCTTCGGCGACGGCATGTATAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCtttt                                                                                                                            >  1:301400/1‑150 (MQ=255)
                   gACAGGATCGCGTACTGATTCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTTCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCttt                                                                                                                             <  1:1102471/149‑1 (MQ=255)
                   gACAGGATCGCGTACTGATTCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTTCGTCTAGTGCTCTAAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCttt                                                                                                                             >  2:1102471/1‑149 (MQ=255)
                   gACAGGATCGCGTACTGATTCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCtttt                                                                                                                            >  2:2266743/1‑150 (MQ=255)
                   gACAGGATCGCGTACTGATTCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCtttt                                                                                                                            <  2:1468113/150‑1 (MQ=255)
                   gACAGGATCGCGTACTGATTCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACAATTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCtttt                                                                                                                            >  1:1144757/1‑150 (MQ=255)
                    aCAGGATCGCGTACTGATTCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTtc                                                                                                                           >  2:1641222/1‑150 (MQ=255)
                     cAGGATCGCGTACTGATTCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTCGAAAAGCAGTGCGAGGCCGAATGTAACACATGCTTTTTTCTGCGTCTAGTGCTATGAAAACGAAAA‑TCACCGAATTTGCGGGGCCTTTtca                                                                                                                          >  2:780997/1‑150 (MQ=255)
                        gATCGCGTACTGATTCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑Gc                                                                                                                                                 >  2:163759/1‑124 (MQ=255)
                        gATCGCGTACTGATTCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑Gc                                                                                                                                                 <  1:163759/124‑1 (MQ=255)
                        gATCGCGTACTGATTCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACAATTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTtcacat                                                                                                                       <  2:1144757/150‑1 (MQ=255)
                           cgcgTACTGATTCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTtcacatcat                                                                                                                    <  1:1265361/150‑2 (MQ=255)
                              gTACTGATTCGAATTAGGGAAGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAgg                                                                                                                 <  2:2420463/150‑1 (MQ=255)
                              gTACTGATTCGAATGAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTCATCTCCGTCTATTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTGTTCACATCACAgg                                                                                                                 >  2:323205/1‑150 (MQ=255)
                                   gATTCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGc                                                                                                            <  1:353373/150‑1 (MQ=255)
                                    aTTCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCTCTCATTTGCGGGGCCTTTTCACATCACAGGTGGGCg                                                                                                           >  2:1772706/1‑150 (MQ=255)
                                     ttCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGGCCCCGCAAATGCGGGGct                                                                                                                                >  1:1935045/1‑128 (MQ=255)
                                     ttCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGGCCCCGCAAATGCGGGGct                                                                                                                                <  2:1935045/129‑2 (MQ=255)
                                        gAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGa                                                                                                       <  1:900303/150‑1 (MQ=255)
                                        gAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGa                                                                                                       >  1:1103496/1‑150 (MQ=255)
                                             aGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAAt                                                                                                  <  1:2452270/150‑1 (MQ=255)
                                               ggATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGc                                                                                                <  2:1103496/150‑1 (MQ=255)
                                                gATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCg                                                                                               >  2:108009/1‑150 (MQ=255)
                                                gATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCg                                                                                               <  2:2087768/150‑1 (MQ=255)
                                                 aTGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCgg                                                                                              <  2:1011570/150‑1 (MQ=255)
                                                  tGCTTCGGCGACGGCATGTATAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGc                                                                                             <  2:301400/150‑1 (MQ=255)
                                                   gCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGc                                                                                                            >  2:560039/1‑134 (MQ=255)
                                                   gCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGc                                                                                                            <  1:560647/134‑1 (MQ=255)
                                                   gCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGc                                                                                                            >  2:560647/1‑134 (MQ=255)
                                                    cTTCGGCGACGGCAAGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAAAACA‑GCAGAGAGTTTTCGGGGCCTTTTCACATCACATGTGGGCGTCGAGGAATGCGGCCa                                                                                           >  2:665213/1‑150 (MQ=255)
                                                           gACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGa                                                                                    >  1:435559/1‑150 (MQ=255)
                                                                 aTGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGa                                                                              >  2:48011/1‑150 (MQ=255)
                                                                    tAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGTTTTGGACAg                                                                           >  1:365856/1‑150 (MQ=255)
                                                                            aCTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCAAATGCGGGGCtttt                                                                                                                             <  2:1134626/92‑1 (MQ=255)
                                                                            aCTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCAAATGCGGGGCtttt                                                                                                                             >  1:1134626/1‑92 (MQ=255)
                                                                              tGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAAcc                                                                 >  2:174096/1‑150 (MQ=255)
                                                                               ggAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCgggg                                                                                                                                  >  2:28049/1‑84 (MQ=255)
                                                                               ggAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCgggg                                                                                                                                  <  1:28049/84‑1 (MQ=255)
                                                                               ggAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCt                                                                <  2:365856/150‑1 (MQ=255)
                                                                                gAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCgg                                                                                                                                    >  1:1287830/1‑81 (MQ=255)
                                                                                 aaaaGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTtg                                                              <  1:1197958/150‑1 (MQ=255)
                                                                                 aaaaGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTtg                                                              <  2:1667461/150‑1 (MQ=255)
                                                                                  aaaGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTtgg                                                             <  1:108009/150‑1 (MQ=255)
                                                                                  aaaGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTtgg                                                             >  1:333402/1‑150 (MQ=255)
                                                                                  aaaGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTtgg                                                             <  1:1641222/150‑1 (MQ=255)
                                                                                  aaaGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTATGCGTCTAGAGCTCTGAAAAAGACAA‑GAAACGCATTTGCACGTCCTTGGCACAGCACTGTTTGGGGTCTAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTtgg                                                             >  2:865342/1‑150 (MQ=255)
                                                                                   aaGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTtggt                                                            <  1:154432/150‑1 (MQ=255)
                                                                                   aaGCAGTGCCCGGCCGAATGTAACTCGTTCTTGTTTCGGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCCCTTGTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTtggt                                                            <  1:413085/150‑1 (MQ=255)
                                                                                    aGCAGTGCGAGGCCGAATGTAACTCTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGNGTCGNGGAATGCGGCCAGCNGCGATTTGGACAGTGCGCCAACCTtggtg                                                           <  1:174096/150‑1 (MQ=255)
                                                                                      cAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGc                                                         >  2:1292407/1‑150 (MQ=255)
                                                                                       aGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGa                                                                                                       <  1:1015908/103‑1 (MQ=255)
                                                                                       aGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGa                                                                                                       >  2:1015908/1‑103 (MQ=255)
                                                                                        gTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCtt                                                       <  2:435559/150‑1 (MQ=255)
                                                                                        gTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCt                                                        >  2:1513238/1‑149 (MQ=255)
                                                                                        gTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCt                                                        <  1:1513238/149‑1 (MQ=255)
                                                                                            taGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACGGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCATCCATGGTGGCTTCGAc                                                   <  2:1919738/149‑1 (MQ=255)
                                                                                               gCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAAt                                                                                                  >  2:1338830/1‑100 (MQ=255)
                                                                                               gCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAAt                                                                                                  <  1:1338830/100‑1 (MQ=255)
                                                                                                ccGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTg                                               <  1:1171002/150‑1 (MQ=255)
                                                                                                       tAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTCt                                        <  1:2184249/150‑1 (MQ=255)
                                                                                                           acTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTg                                                                                >  1:997046/1‑106 (MQ=255)
                                                                                                             ttGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTtggt                                                            <  1:1702710/124‑1 (MQ=255)
                                                                                                             ttGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTtggt                                                            >  2:1702710/1‑124 (MQ=255)
                                                                                                             ttGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTCTTGAACa                                  >  2:2029799/1‑150 (MQ=255)
                                                                                                                 ttttttCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAAt                                                                                                  <  2:325187/82‑1 (MQ=255)
                                                                                                                 ttttttCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAAt                                                                                                  >  1:325187/1‑82 (MQ=255)
                                                                                                                        tGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGa                                                                              <  1:347251/95‑1 (MQ=255)
                                                                                                                        tGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGa                                                                              >  2:352556/1‑95 (MQ=255)
                                                                                                                        tGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGa                                                                              <  1:352556/95‑1 (MQ=255)
                                                                                                                        tGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTCTTGAACAGCATCAGCGTc                       >  2:1888497/1‑150 (MQ=255)
                                                                                                                         gCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTCTTGAACAGCATCAGCGTCg                      <  1:1281586/150‑1 (MQ=255)
                                                                                                                         gCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTCTTGAACAGCATCAGCGTCg                      <  1:1975670/150‑1 (MQ=255)
                                                                                                                           gTCTAGTGCTCTGAAAAAGACAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTCTTGAACAGCATCAGCGTCggg                    >  2:470031/1‑150 (MQ=255)
                                                                                                                            tCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTCTTGAACAGCATCAGCGTCGGGa                   >  1:1886459/1‑150 (MQ=255)
                                                                                                                             cTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTtggt                                                            >  1:1057400/1‑108 (MQ=255)
                                                                                                                             cTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTtggt                                                            <  2:1057400/108‑1 (MQ=255)
                                                                                                                                   ctctGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTCTTGAACAGCATCAGCGTCGGGATAc                <  2:902294/146‑1 (MQ=255)
                                                                                                                                   ctctGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTCTTGAACAGCATCAGCGTCGGGATAc                >  1:902294/1‑146 (MQ=255)
                                                                                                                                      tGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTc                                                      <  2:1309831/105‑1 (MQ=255)
                                                                                                                                      tGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTc                                                      >  1:1309831/1‑105 (MQ=255)
                                                                                                                                        aaaaaGAAAA‑GCCCCGCATTTGCGGTGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGa                                                                                    <  2:1227249/73‑1 (MQ=255)
                                                                                                                                        aaaaaGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGa                                                                                    >  1:1227249/1‑73 (MQ=255)
                                                                                                                                        aaaaaGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTCTTGAACAGCATCAGCGTCg                      <  2:1677847/135‑1 (MQ=255)
                                                                                                                                        aaaaaGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTCTTGAACAGCATCAGCGTCg                      >  1:1677847/1‑135 (MQ=255)
                                                                                                                                        aaaaaGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTCTTGAACAGCATCAGCGTCGGGATACCACGCacac       >  2:1075449/1‑150 (MQ=255)
                                                                                                                                          aaagaaaa‑gCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTCTTGAACAGCATCAGCGTCGGGATACCACGCACACCg     >  2:1323696/1‑150 (MQ=255)
                                                                                                                                            tgaaaagGCCCCGCAAATGCGGGGCTTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTg                                                                                <  1:535002/73‑1 (MQ=25)
                                                                                                                                            tgaaaagGCCCCGCAAATGCGGGGCTTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTg                                                                                >  2:535002/2‑74 (MQ=25)
                                                                                                                                            tgaaaagGCCCCGCAAATGCGGGGCTTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTg                                                                                >  2:536093/2‑74 (MQ=21)
                                                                                                                                            tgaaaagGCCCCGCAAATGCGGGGCTTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTg                                                                                <  1:536093/73‑1 (MQ=21)
                                                                                                                                             gaaaa‑gCCCCGCATTTGCGGGGCCTTTTCACATCACATGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTCTTGAACAGCATCAGCGTCGGGATACCACGCACACCGTGc  >  1:2191777/1‑150 (MQ=255)
                                                                                                                                              aaaa‑gCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTCTTGAACa                                  >  1:2001268/1‑117 (MQ=255)
                                                                                                                                              aaaa‑gCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTCTTGAACa                                  <  2:2001268/117‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GGCCGCGCCGCTGGATGGCGACAGGATCGCGTACTGATTCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAA‑GCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTCTTGAACAGCATCAGCGTCGGGATACCACGCACACCGTGC  >  NC_002947/5948584‑5948874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑