| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,392,927:1 | +A | 10.7% | intergenic (+91/‑95) | PP_1214 → / → ruvC | transcriptional regulator/crossover junction endodeoxyribonuclease RuvC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,392,927 | 1 | . | A | 10.7% | 201.3 / 18.3 | 56 | intergenic (+91/‑95) | PP_1214/ruvC | transcriptional regulator/crossover junction endodeoxyribonuclease RuvC |
| Reads supporting (aligned to +/- strand): ref base . (17/33); new base A (2/4); total (19/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
GCAGAACGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGG > NC_002947/1392779‑1393076 | gCAGAACGTCTATTCCAATGCCCAGATTTCCGACGACATCAGTGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGcc‑c < 2:1378853/150‑1 (MQ=255) gAACGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CAtt > 1:2249326/1‑150 (MQ=255) tCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCaaa < 2:1050888/150‑1 (MQ=255) aTTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAAtgt > 1:1151251/1‑150 (MQ=255) aTTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTACTGCACCACATTTGAAAtg > 1:451406/1‑150 (MQ=37) aTGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCa < 2:1151251/150‑1 (MQ=255) aGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCt < 2:2175158/150‑1 (MQ=255) atgcgcgagccggggtcgatacctagaatcagagtcataaagcccgtcgaaaaaatgatgcatctctacaaactgcGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGa > 1:2194299‑M1/77‑150 (MQ=255) gacgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTAAAAATCTTGTTCATTACCTTTAGGAGc < 2:2040650/150‑1 (MQ=38) acgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCg < 1:1169325/150‑1 (MQ=255) acgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCg > 2:1847056/1‑150 (MQ=255) acgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTACTGCACCACATTTGAAATG‑GGCGTAATACCTGTAGGAGCg < 2:451406/150‑1 (MQ=18) cgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCgg < 1:2415181/150‑1 (MQ=255) gagaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCACCTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAATCCCGCTCCTACAGGTACTGCACCACTTTTGAAATG‑GGCGTAATACCTGTAGGAGCggg < 1:1260162/150‑1 (MQ=17) gagaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCggg < 2:2249326/150‑1 (MQ=255) gagaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCggg > 2:1733917/1‑150 (MQ=255) gagaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCggg < 1:2325547/150‑1 (MQ=255) agaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGt < 2:1399202/150‑1 (MQ=255) agaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGt < 1:1692242/150‑1 (MQ=255) agaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGt < 1:1847056/150‑1 (MQ=255) gtctaTGGAGACCCTCGGCTAAGGCGACTTGGTTCCTTTTTGCAATTCTTAGCGTTTGATAGCGCCTGTGCCTGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGtt < 2:22975/146‑1 (MQ=25) cATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTAc < 1:1733917/150‑1 (MQ=255) aTGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTACTGCACCACATTTGAAATG‑GGCGTAATACCTGTAGGAGCgg < 2:637471/143‑1 (MQ=17) aTGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTACTGCACCACATTTGAAATG‑GGCGTAATACCTGTAGGAGCgg > 1:637471/1‑143 (MQ=17) gAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCcgc < 1:411020/150‑1 (MQ=255) atacctagaatcagagtcataaagcccgtcgaaaaaatgatgcatctctacaaactgcGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAg > 2:2426848‑M1/59‑133 (MQ=255) atacctagaatcagagtcataaagcccgtcgaaaaaatgatgcatctctacaaactgcGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAg < 1:2426848‑M1/75‑1 (MQ=255) gCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCaaa < 1:2156526/82‑1 (MQ=255) gCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCaaa > 2:2156526/1‑82 (MQ=255) gCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCA‑CATTTGAAAtgggc < 1:928508/76‑4 (MQ=21) gCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCA‑CATTTGAAAtgggc > 2:928508/1‑73 (MQ=21) GCCCTTTCGCGGGTAAACCCGCTCCTACCGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGGGGGTTTACCCGCGAAAGGGCCGGCGCCGTTTGTAGAGATGCATCATTTTTTCGACGGGGTTTATGTCTCTGattcctg < 2:1783160‑M1/150‑4 (MQ=255) CTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTa < 1:2346463‑M1/150‑1 (MQ=255) GCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGAc < 2:342578‑M1/150‑1 (MQ=255) GGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGc < 1:862517‑M1/82‑1 (MQ=255) GGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGc > 2:862517‑M1/1‑82 (MQ=255) GGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGAccc < 1:2064363‑M1/150‑1 (MQ=255) CCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcg < 2:2190912‑M1/150‑1 (MQ=255) CCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgc < 2:938042‑M1/150‑1 (MQ=255) CCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgc > 1:342578‑M1/1‑150 (MQ=255) CCGCTCCTACAGGTACTGCACCACATTTGAAATG‑GGCGTAATACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgc < 2:1341663‑M1/150‑1 (MQ=255) CGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgcg < 2:2174782‑M1/150‑1 (MQ=255) TCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGAc > 1:887431‑M1/1‑105 (MQ=255) TCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGAc < 2:887431‑M1/105‑1 (MQ=255) CTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCa < 1:1419204‑M1/150‑1 (MQ=255) CTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCa < 2:2297205‑M1/150‑1 (MQ=255) TACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCAc < 1:779910‑M1/150‑1 (MQ=255) TATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTa < 2:1563373‑M1/150‑1 (MQ=255) ATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTAt > 2:2064363‑M1/1‑150 (MQ=255) ttACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTa > 2:1567959/1‑116 (MQ=255) ttACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTa < 1:1567959/116‑1 (MQ=255) aCGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTa < 2:519258/71‑1 (MQ=255) aCGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTa > 1:519258/1‑71 (MQ=255) gCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGa < 2:402055/101‑1 (MQ=255) gCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGa > 1:402055/1‑101 (MQ=255) gCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGt > 2:2279609/1‑150 (MQ=255) gCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGt > 1:210967/1‑150 (MQ=255) cc‑cATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTg < 1:1563373/150‑1 (MQ=255) cc‑cATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTg > 1:713798/1‑150 (MQ=255) c‑cATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTgg > 1:95279/1‑150 (MQ=255) | GCAGAACGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGG > NC_002947/1392779‑1393076 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |