BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,469,902	G→T	5.3%	E266* (GAG→TAG)	PP_3083 →	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,469,902	0	G	T	5.3%	361.4 / 4.5	111	E266* (GAG→TAG)	PP_3083	membrane protein
Reads supporting (aligned to +/- strand): ref base G (46/59); new base T (3/3); total (49/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 114 total aligned reads displayed.

CGCATTGGCCTGGGGGCTGCGGTGGGCTGGGCAGTGGGCGCGGTACTGGGTATGGACAGCCTGGAGCGTGCGGTGCTGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGTTCTGGCCTTTTCGCGGGCTTGTCCGTGAAAGGCCAGTGGCAATCACATCATGTTCTGCGGATG  >  NC_002947/3469755‑3470051

cgcATTGGCCTGGGGGCTGCGGTGGGCTGGGCAGTGGGCGCGGTACTGGGTATGGACAGCCTGGCGCGTGCGGTGCTGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCagag                                                                                                                                                      <  1:1334864/150‑1 (MQ=255)

 gcATTGGCCTGGGGGCTGCGGTGGGCTGGGCAGTGGGCGCGGTACTGGGTATGGACAGCCTGGAGCGTGCGGTGCTGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGc                                                                                                                                                     >  2:543688/1‑150 (MQ=255)

 gcATTGGCCTGGGGGCTGCGGTGGGCTGGGCAGTGGGCGCGGTACTGGGTATGGACAGCCTGGAGCGTGCGGTGCTGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGc                                                                                                                                                     >  2:1743833/1‑150 (MQ=255)

   aTTGGCCTGGGGGCTGCGGTGGGCTGGGCAGTGGGCGCGGTACTGGGTATGGACAGCCTGGAGCGTGCGGTGCTGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAg                                                                                                                                                   >  1:1152435/1‑150 (MQ=255)

       gCCTGGGGGGTGCGGTGGGCTGGGCAGGGGGCGCGGTGCTGGGTATGGACGGCCTGGAGCGTGCGGTGCTGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGTGGTGCGGGCCAACCGTGAGCCAGAGCAGGTgg                                                                                                                                               <  1:862557/150‑1 (MQ=255)

        ccTGGGGGCTGCGGTGGGCTGGGCAGTGGGCGCGGTACTGGGTATGGACAGCCTGGAGCGTGCGGTGCTGATGGTGCAGTCGGCGATGCCGGTAGCCGTTC‑TC‑GACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGc                                                                                                                                              <  1:2274709/150‑1 (MQ=255)

                cTGCGGTGGGCTGGGCAGTGGGCGCGGTACTGGGTATGGACAGCCTGGAGCGTGCGGTGCTGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTgg                                                                                                                                      <  1:1729672/150‑1 (MQ=255)

                  gCGGTGGGCTGGGCAGTGGGCGCGGTACTGGGTATGGACAGCCTGGAGCGTGCGGTGCTGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTg                                                                                                                                    <  2:1529252/150‑1 (MQ=255)

                    ggTGGGCTGGGCAGTGGGCGCGGTACTGGGTATGGACAGCCTGGAGCGTGCGGTGCTGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGAt                                                                                                                                  <  2:1953342/150‑1 (MQ=255)

                               cAGTGGGCGCGGTACTGGGTATGGACAGCCTGGAGCGTGCGGTGCTGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACgg                                                                                                                       <  2:1297815/150‑1 (MQ=255)

                                 gTGGGCGCGGTACTGGGTATGGACAGCCTGGAGCGTGCGGTGCTGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTg                                                                                                                     >  1:2078205/1‑150 (MQ=255)

                                      cgcgGTACTGGGTATGGACAGCCTGGAGCGTGCGGTGCTGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTc                                                                                                               <  2:1101903/150‑1 (MQ=255)

                                        cgGTACTGGGTATGGACAGCCTGGAGCGTGCGGTGCTGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGAGTCTAACCTGGTGATGTGTTCTACGGTGC‑TGTCGt                                                                                                             <  1:2410265/150‑1 (MQ=255)

                                          gTACTGGGTATGGACAGCCTGGAGCGTGCGGTGCTGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACg                                                                                                                        >  2:2339132/1‑138 (MQ=255)

                                          gTACTGGGTATGGACAGCCTGGAGCGTGCGGTGCTGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACg                                                                                                                        <  1:2339132/138‑1 (MQ=255)

                                              tGGGTATGGACAGCCTGGAGCGTGCGGTGCTGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCt                                                                                                       <  2:965940/150‑1 (MQ=255)

                                                ggTATGGACAGCCTGGAGCGTGCGGTGCTGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTgg                                                                                                     <  1:1644968/150‑1 (MQ=255)

                                                ggTATGGACAGCCTGGAGCGTGCGGTGCTGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTgg                                                                                                     >  1:1419673/1‑150 (MQ=255)

                                                  tATGGACAGCCTGGAGGTGGCGGTGCTGATGGTGCAGTCGGCGATGCCGGAAGCCATGTGTCTGACTAATTAA‑‑ATTGTAGAGGCCAACCCTGCGCCAGAGCAGGTGGCCCACTTCGTTATGGGTTCTACGGTGC‑TGTCGTTTGCCTGGtt                                                                                                   <  1:543688/150‑1 (MQ=255)

                                                  tATGGACAGCCTGGAGCGTGCGGTGCTGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGtt                                                                                                   <  1:1536301/150‑1 (MQ=255)

                                                  tATGGACAGCCTGGAGCGTGCGGTGCTGATGGTGCAGTCGGCGATGCCGGAAAGCGTGT‑GC‑AACGACCTTGTGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGtt                                                                                                   <  1:1743833/150‑1 (MQ=255)

                                                      gACAGCCTGGAGCGTGCGGTGCTGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCg                                                                                               <  1:2291122/150‑1 (MQ=255)

                                                        cAGCCTGGAGCGTGCGGTGCTGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGt                                                                                             >  2:342869/1‑150 (MQ=255)

                                                           ccTGGAGCGTGCGGTGCTGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGtgct                                                                                          >  2:1396654/1‑150 (MQ=255)

                                                           ccTGGAGCGTGCGGTGCTGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGtgct                                                                                          >  2:1855465/1‑150 (MQ=255)

                                                           ccTGGAGCGTGCGGTGCTGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGtg                                                                                            <  1:66993/148‑1 (MQ=255)

                                                           ccTGGAGCGTGCGGTGCTGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGtg                                                                                            >  2:66993/1‑148 (MQ=255)

                                                            cTGGAGCGTGCGGTGCTGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGtgctg                                                                                         >  1:923342/1‑150 (MQ=255)

                                                             tGGAGCGTGCGGTGCTGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGtgctgc                                                                                        >  2:787791/1‑150 (MQ=255)

                                                                   gTGCGGTGCTGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TG‑AACGACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGANGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCtt                                                                                  <  1:136347/150‑1 (MQ=255)

                                                                    tGCGGTGCTGATGTTTTTGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTCCCCCATCGCGGCGCGCGCCAACGGTGAGCCAGAGCAGGTAGCGAACCTGGTGATGTGTTCTACGGTGC‑TCTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTtg                                                                                 <  2:562225/150‑1 (MQ=255)

                                                                     gCGGTGCTGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTtgg                                                                                >  2:1181991/1‑150 (MQ=255)

                                                                         tGCTGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGTTGTCATCGACTTTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGa                                                                            >  2:1881564/1‑150 (MQ=255)

                                                                         tGCTGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGACCATCACG‑TCGCTTGGTTGCCGGTGCTGCTGGCTtggtggg                                                                            >  2:1379242/1‑149 (MQ=255)

                                                                          gCTGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTATGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGAt                                                                           >  2:570564/1‑150 (MQ=255)

                                                                           cTGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATg                                                                          <  2:1419673/150‑1 (MQ=255)

                                                                            tGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTtggt                                                                               >  1:784463/1‑144 (MQ=255)

                                                                            tGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTtggt                                                                               <  2:784463/144‑1 (MQ=255)

                                                                             gATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTtggt                                                                               <  1:1400813/143‑1 (MQ=255)

                                                                             gATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTtggt                                                                               >  2:1400813/1‑143 (MQ=255)

                                                                             gATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTa                                                                        >  1:1276630/1‑150 (MQ=255)

                                                                             gATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTa                                                                        >  1:2102572/1‑150 (MQ=255)

                                                                              aTGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTaa                                                                       <  2:2001956/150‑1 (MQ=255)

                                                                              aTGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTaa                                                                       <  2:923342/150‑1 (MQ=255)

                                                                                ggTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGc                                                                     <  1:1855465/150‑1 (MQ=255)

                                                                                  tGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCt                                                                   <  1:342785/150‑1 (MQ=255)

                                                                                     aGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGtt                                                                                                            >  2:432995/1‑106 (MQ=255)

                                                                                     aGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGtt                                                                                                            <  1:432995/106‑1 (MQ=255)

                                                                                     aGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGtt                                                                <  2:2078205/150‑1 (MQ=255)

                                                                                     aGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGtt                                                                >  1:819142/1‑150 (MQ=255)

                                                                                       tCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGt                                                                 <  1:101982/147‑1 (MQ=255)

                                                                                       tCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGt                                                                 >  2:101982/1‑147 (MQ=255)

                                                                                        cGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGGGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGTTCTg                                                             <  2:1772744/150‑1 (MQ=255)

                                                                                                  ggTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGTATGTAAGCCt                                                                   <  2:2250247/134‑1 (MQ=255)

                                                                                                  ggTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGTATGTAAGCCt                                                                   >  1:2250247/1‑134 (MQ=255)

                                                                                                    tAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGTTCTGGCCTTTTCGCgg                                                 <  1:1179627/150‑1 (MQ=255)

                                                                                                    tAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGTTCTGGCCTTTTCGCgg                                                 <  2:1587858/150‑1 (MQ=255)

                                                                                                    tAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGTTCTGGCCTTTTCGCgg                                                 <  2:1152435/150‑1 (MQ=255)

                                                                                                      gCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGACGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGTTGGATGTAAGCCTGTTCTGGCCTTTTCGCGGGc                                               <  1:440180/150‑1 (MQ=255)

                                                                                                        cGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCt                                                                                   >  2:74852/1‑112 (MQ=255)

                                                                                                        cGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTtggtg                                                                              >  2:952199/1‑117 (MQ=255)

                                                                                                        cGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGTTCTGGCCTTTTCGCGGGCtt                                             <  1:1992305/150‑1 (MQ=255)

                                                                                                         gtgt‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGTTCTGGCCGTTTCGCGGGCTTg                                            >  2:1296111/1‑150 (MQ=255)

                                                                                                          tgt‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATg                                                                          <  2:1632850/119‑1 (MQ=255)

                                                                                                           gt‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGTGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTCCCGGTGCTGCTGGCTTGGTGGATGTAACCCTGTTCTGGCCTTTTCGCGGGCTTGTc                                          <  2:1252876/150‑1 (MQ=255)

                                                                                                            t‑tC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAGGCGCGTTCTGGCCTTTTcgcg                                                  >  1:2341327/1‑141 (MQ=255)

                                                                                                            t‑tC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGTTCTGGCCTTTTcgcg                                                  <  2:2341327/141‑1 (MQ=255)

                                                                                                               c‑AACTACCTGATGGCGGTGCGGTCCAACCGTGAGCCAGAGCAGGTGGCGAACGTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGTTCTGGCCTTTTCGCGGGCTTGTCAGt                                       <  2:178308/150‑1 (MQ=255)

                                                                                                               c‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGTTGGATGTAAGCCTGTTCTGGCCTTTTCGCGGGCTTGTCCGt                                       <  2:2102572/150‑1 (MQ=255)

                                                                                                                 aaCTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAAc                                                                                                                                          <  1:1724770/51‑1 (MQ=255)

                                                                                                                 aaCTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAAc                                                                                                                                          >  2:1724770/1‑51 (MQ=255)

                                                                                                                 aaCTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGTTCTGGCCTTTTCGCGGGCTTGTCCGTg                                      >  1:706099/1‑150 (MQ=255)

                                                                                                                 aaCTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGCGGCTGTAAGCCTGTTCTGGCCTTTTCGCGGGCTTGTCCGTg                                      >  1:1205205/1‑150 (MQ=255)

                                                                                                                   cTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGTTCTGGCCTTTTCGCGGGCTTGTCCGTGaa                                    >  2:1875621/1‑150 (MQ=255)

                                                                                                                    tACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGTTCTGGCCTTTTcgc                                                   <  2:236120/134‑1 (MQ=255)

                                                                                                                    tACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGTTCTGGCCTTTTcgc                                                   >  1:236120/1‑134 (MQ=255)

                                                                                                                    tACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAACCTGTTCTGGCCTTTTCGCGGGCTTGTCCGTGaaa                                   <  1:1379242/150‑1 (MQ=255)

                                                                                                                      ccTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGTTCTGGCCTTTTCGCGGGCTTGTCCGTGAAAgg                                 >  1:252054/1‑150 (MQ=255)

                                                                                                                         gATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGTTCTGGCCTTTTCGCGGGCTTGTCCGTGAAAGGCCa                              >  1:508503/1‑150 (MQ=255)

                                                                                                                            ggCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTATGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGTTCTGGCCTTTTCGCGGGCTTGTCCGTGAAAGGCCAGTg                           <  1:570564/150‑1 (MQ=255)

                                                                                                                            ggCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGTTCTGGCCTTTTCGCGGGCTTGTCCGTGAAAGGCCAGTg                           <  1:2088165/150‑1 (MQ=255)

                                                                                                                            ggCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGTTATGGCCTTTTCGCGGGCTTGTCCGTGAAAGGCCAGTg                           <  1:787791/150‑1 (MQ=255)

                                                                                                                                gTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGGTGTAAGCCTGTTCTGGCCTTTTCGCGGGCTTGTCCGTGAAAGGCCAGTGGCaa                       >  2:1862340/1‑150 (MQ=255)

                                                                                                                                gTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGTTCTGGCCTTTTCGCGGGCTTGTCCGTGAAAGGCCAGTGGCaa                       >  2:1370910/1‑150 (MQ=255)

                                                                                                                                     ggCCAACCGTGAGCCATAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGTTCTGGCCTTTTCGCGGGCTTGTCCGTGAAAGGCCAGTGGCAATcaca                  <  2:414818/150‑1 (MQ=255)

                                                                                                                                     ggCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTtggt                                                                               >  2:809481/1‑89 (MQ=255)

                                                                                                                                     ggCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTtggt                                                                               <  1:809481/89‑1 (MQ=255)

                                                                                                                                        cAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGTTCTGGCCTTTTCGCGGGCTTGTCCGTGAAAGGCCAGTGGCAATCAcatca               >  1:2118575/1‑150 (MQ=255)

                                                                                                                                         aaCCGTGAGCCATAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGTTCTGGCCTTTTCGCGGGCTTGTc                                          >  1:1366081/1‑122 (MQ=255)

                                                                                                                                         aaCCGTGAGCCATAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGTTCTGGCCTTTTCGCGGGCTTGTc                                          <  2:1366081/122‑1 (MQ=255)

                                                                                                                                            cGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCg                                                                                               >  1:2435896/1‑66 (MQ=255)

                                                                                                                                            cGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGTTCTGGCCTTTTCGCGGGCTTGTCCGTGAAAGGCCAGTGGCAATCACATCATGtt           <  1:1396654/150‑1 (MQ=255)

                                                                                                                                                aGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTGGCTGCCCGGTTTTTCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGTTCTGGCCTTTTCGCGGGCTTGTCCGTGAAAGGCCAGTGGCAATCACATCATGTTCTGc       <  2:1638092/150‑1 (MQ=255)

                                                                                                                                                aGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGTTCTGGCCTTTTCGCGGGCTTGTCCGTGATTTCTGAGTGGCAATCACATCATGTTCTGc       <  1:1881564/150‑1 (MQ=255)

                                                                                                                                                 gCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTaa                                                                       <  1:1442731/85‑1 (MQ=255)

                                                                                                                                                 gCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTaa                                                                       >  2:1442731/1‑85 (MQ=255)

                                                                                                                                                  ccAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGTTCTGGCCTTTTCGCGGGCTTGTCCGTGAAAGGCCAGTGGCAATCACATCATGTTCTGCgg     >  1:812956/1‑150 (MQ=255)

                                                                                                                                                  ccAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGTTCTGGCCTTTTCGCGGGCTTGTCCGTGAAAGGCCAGTGGCAATCACATCATGTTCTGCgg     >  2:1286640/1‑150 (MQ=255)

                                                                                                                                                    agagCAGGTGGCGAACCTGGTGACGTATCCGACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGTTCTGGCCTTTTCGCGGGCTTGTCCGTGAAAGGCCAGTGGCAATCACATCATGTTCTGCGGAt   <  1:1181991/150‑1 (MQ=255)

                                                                                                                                                     gagCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGTTCTGGCCTTTTCGCGGGCTTGTCCGTGAAAGGCCAGTGGCAATCACATCATGTTCTGCGGATg  >  2:1849246/1‑150 (MQ=255)

CGCATTGGCCTGGGGGCTGCGGTGGGCTGGGCAGTGGGCGCGGTACTGGGTATGGACAGCCTGGAGCGTGCGGTGCTGATGGTGCAGTCGGCGATGCCGGTAGCCGTGT‑TC‑AACTACCTGATGGCGGTGCGGGCCAACCGTGAGCCAGAGCAGGTGGCGAACCTGGTGATGTGTTCTACGGTGC‑TGTCGTTTGCCTGGTTGCCGGTGCTGCTGGCTTGGTGGATGTAAGCCTGTTCTGGCCTTTTCGCGGGCTTGTCCGTGAAAGGCCAGTGGCAATCACATCATGTTCTGCGGATG  >  NC_002947/3469755‑3470051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`