Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,730,641 C→T 5.7% L142L (CTG→TTG)  PP_3297 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,730,6410CT 5.7% 252.6 / 3.2 87L142L (CTG→TTG) PP_3297hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (37/44);  new base T (2/3);  total (39/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

ACACTGTTTCACGCGATGCCTGCAGCTATTCGCGAGAGCCTGGCCCTGAACAGCCAGGAACACTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATCCGTTCGTGCTGTGTTACATCGCTGAGGTCCAAGCTTGATCTGCGGCCCAGTAATCGAGGCCCGTGGCCTGG  >  NC_002947/3730497‑3730781
                                                                                                                                                 |                                                                                                                                            
acacTGTTTCACGCGATGCCTGCAGCTATTCGCGAGAGCCTGGCCCTGAACAGCCAGGAACACTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATg                                                                                                                                         >  1:251762/1‑150 (MQ=255)
 cacTGTTTCACGCGATGCCTGCAGCTATTCGCGAGAGCCTGGCCCTGAACAGCCAGGAACACTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATgg                                                                                                                                        >  1:320715/1‑150 (MQ=255)
 cacTGTTTCACGCGATGCCTGCAGCTATTCGCGAGAGCCTGGCCCTGAACAGCCAGGAACACTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATgg                                                                                                                                        <  2:958412/150‑1 (MQ=255)
  acTGTTTCACGCGATGCCTGCAGCTATTCGCGAGAGCCTGGCCCTGAACAGCCAGGAACACTCATTCGATCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATggg                                                                                                                                       >  2:2296554/1‑150 (MQ=255)
  acTGTTTCACGCGATGCCTGCAGCTATTCGCGAGAGCCTGGCCCTGAACAGCCAGGAACACTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATggg                                                                                                                                       >  2:872042/1‑150 (MQ=255)
  acTGTTTCACGCGATGCCTGCAGCTATTCGCGAGAGCCTGGCCCTGAACAGCCAGGAACACTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATggg                                                                                                                                       >  2:688833/1‑150 (MQ=255)
   cTGTTTCACGCGATGCCTGCAGCTATTCGTGGGAGCCTGGCCCTGAACAGCCAGGAACACTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATgggg                                                                                                                                      >  1:1244922/1‑150 (MQ=255)
    tGTTTCACGCGATGCCTGCAGCTATTCGCGAGAGCCTGGCCCTGAACAGCCAGGAACACTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGt                                                                                                                                     <  1:1922186/150‑1 (MQ=255)
    tGTTTCACGCGATGCCTGCAGCTATTCGCGAGAGCCTGGCCCTGAACAGCCAGGAACACTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGt                                                                                                                                     >  1:1797969/1‑150 (MQ=255)
      tttCACGCGATGCCTGCAGCTGTTCGCGAGAGCCTGGCCCTGAACAGCCAGGAACACTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTtg                                                                                                                                   >  2:812868/1‑150 (MQ=255)
          aCGCGATGCCTGCAGCTATTCGCGAGAGCCTGGCCCTGAACAGCCAGGAACACTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTtgctgc                                                                                                                               >  2:967986/1‑150 (MQ=255)
          aCGCGATGCCTGCAGCTATTCGCGAGAGCCTGGCCCTGAACAGCCAGGAACACTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTtgctgc                                                                                                                               <  2:251762/150‑1 (MQ=255)
            gcgATGCCTGCAGCTATTCGCGAGAGCCTGGCCCTGAACAGCCAGGAACACTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCgg                                                                                                                             >  1:1798328/1‑150 (MQ=255)
            gcgATGCCTGCAGCTATTCGCGAGAGCCTGGCCCTGAACAGCCAGGAACACTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCgg                                                                                                                             >  2:2263924/1‑150 (MQ=255)
             cgATGCCTGCAGCTATTCGCGAGAGCCTGGCCCTGAACAGCCAGGAACACTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGt                                                                                                                            <  1:165868/150‑1 (MQ=255)
                tGCCTGCAGCTATTCGCGAGAGCCTGGCCCTGAACAGCCAGGAACACTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGa                                                                                                                         >  1:276870/1‑150 (MQ=255)
                     gCAGCTATTCGCGAGAGCCTGGCCCTGAACAGCCAGGAACACTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTg                                                                                                                    <  1:2263924/150‑1 (MQ=255)
                     gCAGCTATTCGCGAGAGCCTGGCCCTGAACAGCCAGGAACACTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTg                                                                                                                    <  1:1934732/150‑1 (MQ=255)
                              cgcgAGAGCCTGGCCCTGAACACCCAGGAACACTCATTCGAGCGGTATCGCGATGTTTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGtg                                                                                                           <  1:368554/150‑1 (MQ=255)
                                 gagaGCCTGGCCCTGAACAGCCAGGAACACTCATTCGATCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGtggtt                                                                                                        <  1:2296554/150‑1 (MQ=255)
                                 gagaGCCTGGCCCTGAACAGCCAGGAACACTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGtggtt                                                                                                        <  1:2271281/150‑1 (MQ=255)
                                  agagCCTGGCCCTGAACAGCCAGGAACACTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGtggttg                                                                                                       >  1:1698671/1‑150 (MQ=255)
                                  agagCCTGGCCCTGAACAGCCAGGAACACTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGtggttg                                                                                                       <  1:95626/150‑1 (MQ=255)
                                   gagCCTGGCCCTGAACAGCCAGGAACACTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGtggttgg                                                                                                      >  1:1800624/1‑150 (MQ=255)
                                       cTGGCCCTGAACAGCCAGGAACACTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTtac                                                                                                  >  1:1431845/1‑150 (MQ=255)
                                       cTGGCCCTGAACAGCCAGGAACACTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTtac                                                                                                  <  1:2450363/150‑1 (MQ=255)
                                             cTGAACAGCCAGGAACACTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAg                                                                                            <  2:607726/150‑1 (MQ=255)
                                             cTGAACAGCCAGGAACACTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAg                                                                                            <  1:812868/150‑1 (MQ=255)
                                              tGAACAGCCAGGAACACTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAgg                                                                                           >  2:2204886/1‑150 (MQ=255)
                                                aaCAGCCAGGAACACTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGaa                                                                                         <  1:967986/150‑1 (MQ=255)
                                                  cAGCCAGGAACACTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATggg                                                                                                                                       >  1:871133/1‑102 (MQ=255)
                                                  cAGCCAGGAACACTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATggg                                                                                                                                       <  2:871133/102‑1 (MQ=255)
                                                   aGCCAGGAACACTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCACCGTGGTGCCCTACGACAATGAACGa                                                                                      <  2:1797969/150‑1 (MQ=255)
                                                    gCCAGGAACACTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAg                                                                                     >  2:517675/1‑150 (MQ=255)
                                                         gAACACTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCg                                                                                >  2:409974/1‑150 (MQ=255)
                                                              cTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCa                                                                              <  1:2342228/147‑1 (MQ=255)
                                                              cTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCa                                                                              >  2:2342228/1‑147 (MQ=255)
                                                              cTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGa                                                                           >  1:729259/1‑150 (MQ=255)
                                                               tCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGTTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTta                                                                                                   >  1:1978499/1‑125 (MQ=255)
                                                               tCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGTTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTta                                                                                                   >  1:1978846/1‑125 (MQ=255)
                                                               tCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGTTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTta                                                                                                   <  2:1978846/125‑1 (MQ=255)
                                                               tCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGTTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTta                                                                                                   <  2:1978499/125‑1 (MQ=255)
                                                               tCATTCGAGCGGTATCGCGATGTGAACCGTGGCAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAACTTTGCCGATCCAGTGCTGATGGGGTTGCTGCAGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGCACGAGGCTCGCAGGAt                                                                          >  1:1881191/1‑150 (MQ=255)
                                                                 aTTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATcc                                                                        >  1:1658368/1‑150 (MQ=255)
                                                                     gAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTACCTCCTCTG‑CAGACACATATCTGCCGATCCAGTGCTGATGGGGTTGCTGCAGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATCCGTTc                                                                    <  2:1881191/150‑1 (MQ=255)
                                                                        cGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATCCGTTCGTg                                                                 <  2:1658368/150‑1 (MQ=255)
                                                                            aTCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGTTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATCCGTTCGTGCggt                                                             <  1:688833/150‑1 (MQ=255)
                                                                            aTCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATCCGTTCGTGCtgt                                                             <  1:872042/150‑1 (MQ=255)
                                                                             tCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATCCGTTCGTGCtgt                                                             >  1:1090833/1‑149 (MQ=255)
                                                                             tCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATCCGTTCGTGCtgt                                                             <  2:1090833/149‑1 (MQ=255)
                                                                             gcgcgATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTtacta                                                                                                <  1:2188255/113‑1 (MQ=255)
                                                                             gcgcgATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTtacta                                                                                                >  2:2188255/2‑114 (MQ=255)
                                                                                   tgtgTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATCCGTTCGTGCTGTGTTACAt                                                      <  1:517675/150‑1 (MQ=255)
                                                                                       ttCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAGCGAGGCTCGCAGTATCCGTTCGTGCTGTGTTACATCGCt                                                  <  1:2204886/150‑1 (MQ=255)
                                                                                         cACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGGGGCTCGCAGGATCCGTTCGTGCTGTGTTACATCGCTGa                                                >  1:1812390/1‑150 (MQ=255)
                                                                                           ccAACATCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATCCGTTCGTGCTGTGTTACATCGCTGAgg                                              <  1:1121031/150‑1 (MQ=255)
                                                                                           ccAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATCCGTTCGTGCTGTGTTACATCGCTGAgg                                              >  1:1053673/1‑150 (MQ=255)
                                                                                           ccAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATCCGTTCGTGCTGTGTTACATCGCTGAgg                                              <  1:709741/150‑1 (MQ=255)
                                                                                           ccAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATCCGTTCGTGCTGTGTTACATCGCTGAgg                                              >  2:1125367/1‑150 (MQ=255)
                                                                                            cAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGtggttgg                                                                                                      <  1:1116163/93‑1 (MQ=255)
                                                                                            cAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGtggttgg                                                                                                      >  2:1116163/1‑93 (MQ=255)
                                                                                                aGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATCCGTTCGTGCTGTGTTACATCGCTGAGGTCCaa                                         <  1:2437582/150‑1 (MQ=255)
                                                                                                   cGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCAAACTCAGTGAGCACTGCATTGTTGGGTTGTTTGTTGGCTAATGGGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATCCGTTCGTGCTGTGTTACATCGCTGAGGTCCAAGCt                                      <  1:1116895/150‑1 (MQ=255)
                                                                                                    ggTATCAGTA‑CGAGTAGGACCGCTGGACAATTGGCGACCTAGTATAAATGTGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATCCGTTCGTGCTGTGTTACATCGCTGAGGTCCAAGCtt                                     <  2:1204705/150‑1 (MQ=255)
                                                                                                     gTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGt                                                                                                                      <  1:2244803/68‑1 (MQ=255)
                                                                                                     gTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGt                                                                                                                      >  2:2244803/1‑68 (MQ=255)
                                                                                                       aTCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATCCGTTCGTGCtg                                                              >  2:229176/1‑122 (MQ=255)
                                                                                                       aTCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATCCGTTCGTGCtg                                                              <  1:229176/122‑1 (MQ=255)
                                                                                                       aTCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATCCGTTCGTGCTGTGTTACATCGCTGAGGTCCAAGCTTGAt                                  <  2:1431845/150‑1 (MQ=255)
                                                                                                          aGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATCCGTTCGTGCTGTGTTACATCGCTGAGGTCCAAGCTTGAt                                  >  2:1681235/1‑147 (MQ=255)
                                                                                                          aGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATCCGTTCGTGCTGTGTTACATCGCTGAGGTCCAAGCTTGAt                                  <  1:1681235/147‑1 (MQ=255)
                                                                                                           gTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTg                                                                                                                           <  1:84385/57‑1 (MQ=255)
                                                                                                           gTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTg                                                                                                                           >  2:84385/1‑57 (MQ=255)
                                                                                                                gAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATCCGTTCGTGCTGTGTTACATCGCTGAGGTCCAAGCTTGATCTGCGGcc                          <  2:729259/150‑1 (MQ=255)
                                                                                                                  gTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTCGACGTTGGCCAAGGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATCCGTTCGTGCTGTGTTACATCGCTGAGGTCCAAGCTTACTCTGCGGCCCa                        >  2:1328094/1‑150 (MQ=255)
                                                                                                                  gTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGATGCGGTGCACGTTGTCCAACGTGTTTGTTTACTCACAGGAACGAGGCTCAAAGCTTCTTGTCTTTCGTTTTGACATCGCTGAGGTCCAAGCTTGATCTGCGGCCCa                        <  1:1863283/150‑1 (MQ=255)
                                                                                                                      gCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATCCGTTCGTGCTGTGTTACATCGCTGAGGTCCAAGCTTGATCTGCGGCCCAGTaa                    >  2:792722/1‑150 (MQ=255)
                                                                                                                       ccAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATCCGTTCGTGCTGTGTTACATCGCTGAGGTCCAAGCTTGATCTGCGGCCCAGTAAt                   <  1:1950529/150‑1 (MQ=255)
                                                                                                                       ccAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGAAGTAAGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATCCGTTCGTGCTGTGTTACATCGCTGAGGTCCAAGCTTGATCTGCGGCCCAGTAAt                   <  2:1244922/150‑1 (MQ=255)
                                                                                                                            tGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATCCGTTCGTGCTGTGTTACATCGCTGAGGTCCAAGCTTGATCTGCGGCCCAGTAATCGAgg              <  1:409974/150‑1 (MQ=255)
                                                                                                                            tGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATCCGTTCGTGCTGTGTTACATCGCTGAGGTCCAAGCTTGATCTGCGGCCCAGTAATCGAgg              <  1:2164963/150‑1 (MQ=255)
                                                                                                                                 aTTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATCCGTTCGTGCTGTGTTACATCGCTGAGGTCCAAGCTTGCCCTGCGGCCAAGTTATCGTGTcccag         >  2:510570/1‑148 (MQ=255)
                                                                                                                                    tGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATCCGTTCGTGCTGTGTTACATCGCTGAGGTCCAAGCTTGATCTGCGGCCCAGTAATCGAGGCCCGTGGc      <  2:276870/150‑1 (MQ=255)
                                                                                                                                      ccGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATCCGTTCGTGCTGTGTTACATCGCTGAGGTCCAAGCTTGATCTGCGGCCCAGTAATCGAGGCCCGTGGCCt    <  2:1053673/150‑1 (MQ=255)
                                                                                                                                       cGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATCCGTTCGTGCTGTGTTACATCGCTGAGGTCCAAGCTTGATCTGCgg                            >  1:2051932/1‑125 (MQ=255)
                                                                                                                                       cGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATCCGTTCGTGCTGTGTTACATCGCTGAGGTCCAAGCTTGATCTGCgg                            <  2:2051932/125‑1 (MQ=255)
                                                                                                                                        gATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATCCGTTCGTGCTGTGTTACATCGCTGAGGTCCAAGCTTGATCTGCGGCCCAGTAATCGAGGCCCGTGGCCTgg  <  1:1177519/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                            
ACACTGTTTCACGCGATGCCTGCAGCTATTCGCGAGAGCCTGGCCCTGAACAGCCAGGAACACTCATTCGAGCGGTATCGCGATGTGTTCACCAACAGCCGGTATCAGTA‑CGAGTCGGCCAGCTGGAAATTTGCCGATCCAGTGCTGATGGGGTTGCTGCGGTGGACGTTGGCCAACGTGGTTGGTTACTACAAGGAACGAGGCTCGCAGGATCCGTTCGTGCTGTGTTACATCGCTGAGGTCCAAGCTTGATCTGCGGCCCAGTAATCGAGGCCCGTGGCCTGG  >  NC_002947/3730497‑3730781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑