BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,787,762	T→G	8.1%	S306R (AGC→CGC) ‡	PP_3350 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,787,762	0	T	G	8.1%	296.5 / 17.1	112	S306R (AGC→CGC) ‡	PP_3350	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (52/51); new base G (3/6); total (55/57)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.52e-01

Only 100 of 112 total aligned reads displayed.

CTGGCTTCGCCATAGACACCATTGCGTGGGTAAAGCGGGTCGAAGGTGCCGACTTTTCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATGGCGGCCGAACAGCCGCGTGCCGAAAGTGTAGCGCTGCTCGTCACCCGACTCGCCGGCCAGGGT  >  NC_002947/3787614‑3787909

ctggctTCGCCATAGACACCATTGCGTGGGTAAAGCGGGTCGAAGGTGCCGACTTTTCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTc                                                                                                                                                    <  2:1201855/150‑1 (MQ=255)

   gctTCGCCATAGACACCATTGCGTGGGTAAAGCGGGTCGAAGGTGCCGACTTTTCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGAc                                                                                                                                                 >  2:2267352/1‑150 (MQ=255)

   gctTCGCCATAGACACCATTGCGTGGGTAAAGCGGGTCGAAGGTGCCGACTTTTCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGAc                                                                                                                                                 >  2:1811125/1‑150 (MQ=255)

    ctTCGCCATAGACACCATTGCGTGGGTAAAGCGGGTCGAAGGTGCCGACTTTTCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACa                                                                                                                                                <  2:208594/150‑1 (MQ=255)

        gCCATAGACACCATTGCGTGGGTAAAGCGGGTCGACGGTGCCAACTTTTCCTCCGCCCAGGTCACTGTCGCCGCTTGGGGCATCGATGCGAATGGCCAGGCGCGCGTGCCAGGGGTGGGTGAAGGGGAAGCCGCTGTCGCTCGACAgcgc                                                                                                                                            >  1:1879717/1‑150 (MQ=255)

        gCCATAGACACCATTGCGTGGGTAAAGCGGGTCGAAGGTGCCGACTTTTCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAgcgc                                                                                                                                            >  1:60053/1‑150 (MQ=255)

        gCCATAGACACCATTGCGTGGGTAAAGCGGGTCGAAGGTGCCGACTTTTCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCAGCTGTTGAGCGACAgcgt                                                                                                                                            <  2:1731857/150‑2 (MQ=255)

         ccATAGACACCATTGCGTGGGTAAAGCGGGTCGAAGGTGCCGACTTTTCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGcc                                                                                                                                           >  1:212484/1‑150 (MQ=255)

         ccATAGACACCATTGCGTGGGTAAAGCGGGTCGAAGGTGCCGACTTTTCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGcc                                                                                                                                           >  2:1621174/1‑150 (MQ=255)

         ccATAGACACCATTGCGTGGGTAAAGCGGGTCGAAGGTGCCGACTTTTCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGcc                                                                                                                                           >  2:708026/1‑150 (MQ=255)

         ccATAGACACCATTGCGTGGGTAAAGCGGGTCGAAGGTGCCGACTTTTCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGcc                                                                                                                                           >  2:1481012/1‑150 (MQ=255)

           aTAGACACCATTGCGTGGGTAAAGCGGGTCGAAGGTGCCGACTTTTCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGAGCGACAgcggctt                                                                                                                                         <  2:1536350/150‑5 (MQ=255)

              gACACCATTGCGTGGGTAAAGCGGGTCGAAGGTGCCGACTTTTCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTTTCGCTCGACAGCGCCCAGgc                                                                                                                                      >  2:1134730/1‑150 (MQ=255)

              gACACCATTGCGTGGGTAAAGCGGGTCGAAGGTGCCGACTTTTCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGgc                                                                                                                                      <  1:1127065/150‑1 (MQ=255)

               acacCATTGCGTGGGTAAAGCGGGTCGAAGGTGCCGACTTTTCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGgcg                                                                                                                                     <  1:679440/150‑1 (MQ=255)

                 acCATTGCGTGGGTAAAGCGGGTCGAAGATGCCGACTTTTCCTTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCCATGCGGATGTCCATGCGCGGTTGCCCGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCCGGcgcg                                                                                                                                   >  1:917906/1‑150 (MQ=255)

                   cATTGCGTGGGTAAAGCGGGTCGAAGGTGCCGACTTTTCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGa                                                                                                                                 >  1:39724/1‑150 (MQ=255)

                     ttGCGTGGGTAAAGCGGGTCGAAGGTGCCGACTTTTCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATc                                                                                                                               >  1:463222/1‑150 (MQ=255)

                       gCGTGGGTAAAGCGGGTCGAAGGTGCCGACTTTTCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGa                                                                                                                             <  2:39724/150‑1 (MQ=255)

                        cGTGGGTAAAGCGGGTCGAAGGTGCCGACTTTTCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGAc                                                                                                                            <  1:522395/150‑1 (MQ=255)

                         gTGGGTAAAGCGGGTCGAAGGTGCCGACTTTTCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGAcg                                                                                                                           >  1:1201927/1‑150 (MQ=255)

                          tGGGTAAAGCGGGTCGAAGGTGCCGACTTTTCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGAcgc                                                                                                                          >  1:2397675/1‑150 (MQ=255)

                           gggTAAAGCGGGTCGAAGGTGCCGACTTTTCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGAcgcg                                                                                                                         >  2:1414274/1‑150 (MQ=255)

                            ggTAAAGCGGGTCGAAGGTGCCGACTTTTCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGt                                                                                                                        <  1:708026/150‑1 (MQ=255)

                              tAAAGCGGGTCGAAGGTGCCGACTTTTCAGTGGTGCAGGGCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTg                                                                                                                      <  2:188954/150‑1 (MQ=255)

                                  gCGGGTCGAAGGTGCCGACTTTTCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAgcgg                                                                                                                                            <  1:1749208/124‑2 (MQ=255)

                                  gCGGGTCGAAGGTGCCGACTTTTCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAgcgg                                                                                                                                            >  2:1749208/1‑123 (MQ=255)

                                   cGGGTCGAAGGTGCCGACTTTTCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAg                                                                                                                 <  1:2267352/150‑1 (MQ=255)

                                   cGGGTCGAAGGTGCCGACTTTTCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAg                                                                                                                 <  2:2397675/150‑1 (MQ=255)

                                     ggTCGAAGGTGCCGACTTTTCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGt                                                                                                               <  1:773946/150‑1 (MQ=255)

                                         gAAGGTGCCGACTTTTCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGcc                                                                                                           >  1:1559331/1‑150 (MQ=255)

                                            ggTGCCGACTTTTCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGt                                                                                                        <  1:1134730/150‑1 (MQ=255)

                                                   aCTTTTCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTc                                                                                                       <  1:853912/144‑1 (MQ=255)

                                                   aCTTTTCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTc                                                                                                       >  2:853912/1‑144 (MQ=255)

                                                     ttttCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCcgac                                                                                               >  1:80184/1‑150 (MQ=255)

                                                       ttCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCcgacga                                                                                             <  1:1414274/150‑1 (MQ=255)

                                                        tCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAg                                                                                            <  1:1274349/150‑1 (MQ=255)

                                                          cGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCATGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGt                                                                                          >  1:2091191/1‑150 (MQ=255)

                                                          cGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGt                                                                                          >  1:1269857/1‑150 (MQ=255)

                                                          cGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGt                                                                                          >  2:2427983/1‑150 (MQ=255)

                                                           gTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGtt                                                                                         <  1:2361317/150‑1 (MQ=255)

                                                             cGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTcc                                                                                       <  2:782035/150‑1 (MQ=255)

                                                                ccAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGc                                                                                    <  2:1559331/150‑1 (MQ=255)

                                                                ccAGGTCACTGTCGCCCCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGc                                                                                    >  2:1221693/1‑150 (MQ=255)

                                                                 cAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGcc                                                                                                           <  1:589731/126‑1 (MQ=255)

                                                                 cAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGcc                                                                                                           >  2:589731/1‑126 (MQ=255)

                                                                    gTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCtcca                                                                                <  1:205233/150‑1 (MQ=255)

                                                                       aCTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAgg                                                                                           <  1:911666/136‑1 (MQ=255)

                                                                       aCTGTCGCCGCTTGCGGAATCGCTGTGTAATGCCAGGTTTTGTTGCCAGGTGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCtccagtc                                                                             <  2:1839982/150‑1 (MQ=255)

                                                                         tGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCtccagtcca                                                                           >  2:1561604/1‑150 (MQ=255)

                                                                          gTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCtccagtccag                                                                          <  1:1561604/150‑1 (MQ=255)

                                                                           tCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTccagtccagt                                                                         <  2:1391602/150‑1 (MQ=255)

                                                                             gccgcTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATGGAGGCGGTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTgg                                                                       >  2:2276264/1‑149 (MQ=255)

                                                                             gccgcTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGc                                                                       >  2:396972/1‑150 (MQ=255)

                                                                              ccgcTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGcc                                                                      <  1:1811125/150‑1 (MQ=255)

                                                                               cgcTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCt                                                                     <  1:1691041/150‑1 (MQ=255)

                                                                                 cTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGa                                                                   <  2:80184/150‑1 (MQ=255)

                                                                                   ggCGGCATCGATGCGCAGGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCGGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATg                                                                 <  2:49863/149‑1 (MQ=255)

                                                                                     cGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCAGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATggc                                                               <  1:2149922/150‑1 (MQ=255)

                                                                                      ggCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATggcg                                                              <  2:2177830/150‑1 (MQ=255)

                                                                                         aTCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGt                                                                                                               <  1:2035463/98‑1 (MQ=255)

                                                                                         aTCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGt                                                                                                               >  2:2035463/1‑98 (MQ=255)

                                                                                            gATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATGGCGGCCGaa                                                        >  2:2449165/1‑150 (MQ=255)

                                                                                            gATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATGGCGGCCGaa                                                        >  2:1524560/1‑150 (MQ=255)

                                                                                                cgcATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATGGCGGCCGAACAGc                                                    <  1:851067/150‑1 (MQ=255)

                                                                                                 gcATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATGGCGGCCGAACAGcc                                                   <  2:1686636/150‑1 (MQ=255)

                                                                                                 gcATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGCCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATGGCGGCCGCACAGcc                                                   >  1:47868/1‑150 (MQ=255)

                                                                                                  cATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATGGCGGCCGAACAGCcg                                                  >  1:4112/1‑150 (MQ=255)

                                                                                                      gCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCg                                                                                                          >  1:1874809/1‑90 (MQ=255)

                                                                                                      gCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCg                                                                                                          <  2:1874809/90‑1 (MQ=255)

                                                                                                        cAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATGGCGGCCGAACAGCCGCGTGcc                                            >  1:868400/1‑150 (MQ=255)

                                                                                                        cAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAACTCCAGTCCAGTGCCTGATGGCGGCCGAACAGCCGCGTGcc                                            >  1:78115/1‑150 (MQ=255)

                                                                                                        cAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCACGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATGGCGGCCGAACAGCCGCGTGcc                                            >  2:1643230/1‑150 (MQ=255)

                                                                                                         aGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATggc                                                               >  2:477764/1‑130 (MQ=255)

                                                                                                         aGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATggc                                                               <  1:477764/130‑1 (MQ=255)

                                                                                                            cgcgGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATGGCGGCCGAACAGCCGCGTGCCGaaa                                        <  1:1221693/150‑1 (MQ=255)

                                                                                                                gTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATGGCGGCCGAACAGCCGCGTGCCGAAAgtgt                                    <  1:2427983/150‑1 (MQ=255)

                                                                                                                   gCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATGGCGGCCGAACAGCCGCGTGCCGAAAGTGTAgc                                 >  2:1022306/1‑150 (MQ=255)

                                                                                                                     cAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGa                                                                   <  1:2435312/114‑1 (MQ=255)

                                                                                                                     cAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGa                                                                   >  2:2435312/1‑114 (MQ=255)

                                                                                                                     cAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATGGCGGCCGCACAGCCGCGTGCCGAAAGTGTAgcgc                               >  1:927577/1‑150 (MQ=255)

                                                                                                                       ggggTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATGGCGGCCGAACAGCCGCGTGCCGAAAGTGTAGCgctg                             <  1:396972/150‑1 (MQ=255)

                                                                                                                          gtgggtgAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATGGCGGCCGAACAGCCGCGTGCCGAAAg                                       <  2:890362/137‑1 (MQ=255)

                                                                                                                          gtgggtgAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATGGCGGCCGAACAGCCGCGTGCCGAAAg                                       >  1:890362/1‑137 (MQ=255)

                                                                                                                          gtgggtgAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATGGCGGCCGAACAGCCGCGTGCCGAAAGTGTAGCGCTGCTc                          <  1:1022306/150‑1 (MQ=255)

                                                                                                                          gtgggtgAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATGGCGGCCGAACAGCCGCGTGCCGAAAGTGTAGCGCTGCTc                          <  2:2305012/150‑1 (MQ=255)

                                                                                                                              gtgaaggtgaagCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATGGCGGCCGAACAGCCGCGTGCCGAAAGTGTa                                   >  1:1171606/1‑137 (MQ=255)

                                                                                                                              gtgaaggtgaagCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATGGCGGCCGAACAGCCGCGTGCCGAAAGTGTa                                   <  2:1171606/137‑1 (MQ=255)

                                                                                                                                  aggtgaagCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATGGCGGCCGAACAGCCGCGTGCCGAAAGTGTAGCGCTGCTCGTCACCCg                  >  1:1027044/1‑150 (MQ=255)

                                                                                                                                      gaagCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATGGCGGCCGAACAGCCGCGTGCCGAAAGTGTa                                   <  1:2233580/129‑1 (MQ=255)

                                                                                                                                      gaagCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATGGCGGCCGAACAGCCGCGTGCCGAAAGTGTa                                   >  2:2233580/1‑129 (MQ=255)

                                                                                                                                      ctgggcGCTGTCGAGCGACAGCGCCCAGGCGCGGATCGACGCGTTGCGCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATGGCGGCCGAACAGCCGCGTGCCGAAAGTGTAGCGCTGCTCGTCACCCGACTc              >  2:1225254/6‑150 (MQ=255)

                                                                                                                                         gCCGCTGTCGCTCGACAGCGCCCAGGGGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATGGCGGCCGAACAGCCGCGTGCCGAAAGTGTAGCGCTGCTCGTCACCCGACTCGcc           >  1:2157554/1‑150 (MQ=255)

                                                                                                                                          gcGCTGTCGAGCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATggcggc                                                            <  1:1570408/99‑1 (MQ=255)

                                                                                                                                          gcGCTGTCGAGCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATggcggc                                                            >  2:1570408/2‑100 (MQ=255)

                                                                                                                                          gcGCTGTCGAGCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATGGCGGCCGAACAGCCGCGTGCCGAAAGTGTAGCGCTGCTCGt                        >  1:88770/2‑136 (MQ=255)

                                                                                                                                          gcGCTGTCGAGCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATGGCGGCCGAACAGCCGCGTGCCGAAAGTGTAGCGCTGCTCGt                        <  2:88770/135‑1 (MQ=255)

                                                                                                                                                  gCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATGGCGGCCGAACAGCCGCGTGCCGAAAGTGTAGCGCTGCTCGTCACCCGACTCGCCGGCCAgggg  >  2:826831/1‑149 (MQ=255)

                                                                                                                                                   cTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATGGCGGCCGAACAGCCGCGTGCCGaaa                                        >  1:1732477/1‑111 (MQ=255)

                                                                                                                                                   cTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATGGCGGCCGAACAGCCGCGTGCCGaaa                                        <  2:1732477/111‑1 (MQ=255)

CTGGCTTCGCCATAGACACCATTGCGTGGGTAAAGCGGGTCGAAGGTGCCGACTTTTCCGTCGCCCAGGTCACTGTCGCCGCTTGCGGCATCGATGCGCATGGCCAGGCGCGGTTGCCAGGGGTGGGTGAAGGTGAAGCCGCTGTCGCTCGACAGCGCCCAGGCGCGGATCGACGCGTTGCCCAGGTGGCCGGTCTGCCCGACGAGGTTCCAGCTCCAGTCCAGTGCCTGATGGCGGCCGAACAGCCGCGTGCCGAAAGTGTAGCGCTGCTCGTCACCCGACTCGCCGGCCAGGGT  >  NC_002947/3787614‑3787909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`