Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,914,733 C→G 11.3% R33P (CGG→CCG)  PP_3454 ← transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,914,7330CG11.3% 351.3 / 29.9 116R33P (CGG→CCG) PP_3454transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (45/57);  new base G (10/3);  total (55/61)
Fisher's exact test for biased strand distribution p-value = 3.75e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 123 total aligned reads displayed.

CGCGGGCCGTCAGCATGAGGATTGGCAGGGTCTGCGACTCCTGGCGCAGCAGCCGGCACAGCTGCAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGAAGTAAGCCTGTGGCGAGCGCTTCGCGGG  >  NC_002947/3914584‑3914881
                                                                                                                                                     |                                                                                                                                                    
cgcgGGCCGTCAGCATGAGGATTGGCAGGGTCTGCGACTCCTGGCGCAGCAGCCGGCACAGCTGCAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGcccc                                                                                                                                                      <  2:1180331/150‑1 (MQ=255)
      ccGTCAGCATGAGGATTGGCAGGGTCTGCGACTCCTGGCGCAGCAGCCGGCACAGCTGCAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGa                                                                                                                                                <  1:1940589/150‑1 (MQ=255)
       cGTCAGCATGAGGATTGGCAGGGTCTGCGACTCCTGGCGCAGCAGCCGGCACAGCTGCAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGAt                                                                                                                                               <  2:1230767/150‑1 (MQ=255)
        gTCAGCATGAGGATTGGCAGGGTCTGCGACTCCTGGCGCAGCAGCCGGCACAGCTGCAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCTGCACATGATCGCCCCGGGCGAtg                                                                                                                                              >  2:1761834/1‑149 (MQ=255)
         ccAGCATGAGGATTGGCAGGGTCTGCGACTCCTGGCGCGGCAGCCTGCACGGCTGCAGACCATCGATGCCCGGCGGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCa                                                                                                                                             >  1:92011/2‑150 (MQ=255)
          cAGCATGAGGATTGGCAGGGTCTGCGACTCCTGGCGCAGCAGCCGGCACAGCTGCAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCGGGGCGATCat                                                                                                                                            <  2:469441/150‑2 (MQ=255)
           aGCATGAGGATTGGCAGGGTCTGCGACTCCTGGCGCAGCAGCCGGCACAGCTGCAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCggg                                                                                                                                                   <  2:898354/142‑1 (MQ=255)
           aGCATGAGGATTGGCAGGGTCTGCGACTCCTGGCGCAGCAGCCGGCACAGCTGCAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCggg                                                                                                                                                   >  1:898354/1‑142 (MQ=255)
           aGCATGAGGATTGGCAGGGTCTGCGACTCCTGGCGCAGCAGCCGGCACAGCTGCAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACc                                                                                                                                           <  2:490118‑M2/150‑2 (MQ=255)
            gCATGAGGATTGGCAGGGTCTGCGACTCCTGGCGCAGCAGCCGGCACAGCTGCAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACct                                                                                                                                          >  1:750412‑M2/1‑148 (MQ=255)
              aTGAGGATTGGCAGGGTCTGCGACTCCTGGCGCAGCAGCCGGCACAGCTGCAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGCTCGCCCCGGGCGATCCCctgg                                                                                                                                        <  2:105232‑M2/150‑5 (MQ=255)
                gAGGATTGGCAGGGTCTGCGACTCCTGGCGCAGCAGCCGGCACAGCTGCAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACctggac                                                                                                                                      <  1:840674‑M2/150‑7 (MQ=255)
                    aTTGGCAGGGTCTGCGACTCCTGGCGCAGCAGCCGGCACAGCTGCAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATgg                                                                                                                                  >  2:217994/1‑150 (MQ=255)
                    aTTGGCAGGGTCTGCGACTCCTGGCGCAGCAGCCGGCACAGCTGCAGGCCATCGATGCCCGGCAGAATCAGCCTTCGCCGCACCAGGCCGCTTCGTCGCTGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATgg                                                                                                                                  <  1:1486323/150‑1 (MQ=255)
                      tGGCAGGGTCTGCGACTCCTGGCGCAGCAGCCGGCACAGCTGCAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGaa                                                                                                                                <  1:1385426/150‑1 (MQ=255)
                      tGGCAGGGTCTGCGACTCCTGGCGCAGCAGCCGGCACAGCTGCAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGaa                                                                                                                                <  1:1435237/150‑1 (MQ=255)
                              tCTGCGACTCCTGGCGCAGCAGCCGGCACAGCTGCAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTcgttgc                                                                                                                        >  2:891412/1‑150 (MQ=255)
                                tGCGACTCCTGGCGCAGCAGCCGGCACAGCTGCAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCGGGGCGATCat                                                                                                                                            <  2:563572/128‑2 (MQ=255)
                                tGCGACTCCTGGCGCAGCAGCCGGCACAGCTGCAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCGGGGCGATCat                                                                                                                                            >  1:563572/1‑127 (MQ=255)
                                 gCGACTCCTGGCGCAGCAGCCGGCACAGCTGCAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGG‑‑‑TGATCGCCCGGGGCGATCATgtgctggacgagtaccgtcggcaaag                                                                                                                  >  1:1398958‑M2/1‑124 (MQ=255)
                                  cGACTCCTGGCGCAGCAGCCGGCGCAGCTGCAGGCCATCGATGCCCGGCCGCATCAGGACAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTcgttgcgttg                                                                                                                    >  2:1041122/1‑150 (MQ=255)
                                   gACTCCTGGCGCAGCAGCCGGCACAGCTGCAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCgttgcgttgc                                                                                                                   >  1:1992634/1‑150 (MQ=255)
                                   gACTCCTGGCGCAGCAGCCGGCACAGCTGCAGGCCATCGATGCCAGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCgttgcgttgc                                                                                                                   >  1:1992343/1‑150 (MQ=255)
                                    ccTCCTGGCGCAGCAGCCGGCACAGCTGCAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCa                                                                                                                  <  2:2380504/149‑1 (MQ=255)
                                        cTGGCGCAGCAGCCGGCACAGCTGCAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGCTGACCAGGTCAGGCCCGTGCCGAGGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTa                                                                                                              >  1:884713/1‑150 (MQ=255)
                                        cTGGCGCAGCAGCCGGCACAGCTGCAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGaa                                                                                                                                >  1:1736500/1‑132 (MQ=255)
                                         tGGCGCAGCAGCCGGCACAGCTGCAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAg                                                                                                             >  2:1312782/1‑150 (MQ=255)
                                          ggCGCAGCAGCCGGCACAGCTGCAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGc                                                                                                            >  1:588249/1‑150 (MQ=255)
                                           gcgcAGCAGCCGGCACAGCTGCAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAg                                                                                                                 >  1:1854765/1‑144 (MQ=255)
                                           gcgcAGCAGCCGGCACAGCTGCAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAg                                                                                                                 <  2:1854765/144‑1 (MQ=255)
                                            cgcAGCAGCCGGCACAGCTGCAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCAAGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTg                                                                                                          <  1:1761834/150‑1 (MQ=255)
                                               agcagcCGGCACAGCTGCAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCg                                                                                                       >  2:2384974/1‑150 (MQ=255)
                                                   gcCGGCACAGCTGCAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCa                                                                                                   <  1:1269657/150‑1 (MQ=255)
                                                       gCACAGCTGCAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTc                                                                                               <  2:1930286/150‑1 (MQ=255)
                                                              tGCAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAgg                                                                                        <  1:2384974/150‑1 (MQ=255)
                                                               gCAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAggg                                                                                       >  2:119158/1‑150 (MQ=255)
                                                                cAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGgc                                                                                      >  2:778454/1‑150 (MQ=255)
                                                                  ggCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGgcgc                                                                                    >  1:96367/1‑150 (MQ=255)
                                                                   gCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGa                                                                                                                                                <  2:651291/89‑1 (MQ=255)
                                                                   gCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGa                                                                                                                                                >  1:651291/1‑89 (MQ=255)
                                                                    ccATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTAGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTg                                                                                  >  1:1830085/1‑150 (MQ=255)
                                                                     cATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTgt                                                                                 >  1:750447/1‑150 (MQ=255)
                                                                           tGCCCGGCAGCATCAGGTCAAGGATGACCATGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTCGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCtt                                                                           <  2:1278493/150‑1 (MQ=255)
                                                                           tGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCtt                                                                           <  2:1448439/150‑1 (MQ=255)
                                                                              ccGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGa                                                                        >  2:491964/1‑150 (MQ=255)
                                                                               cGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGAc                                                                       <  2:757497/150‑1 (MQ=255)
                                                                                   agcaTCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGcccc                                                                                                                                                      >  1:1278196/1‑67 (MQ=255)
                                                                                     caTCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCGGGGCGATCat                                                                                                                                            >  1:2419042/1‑74 (MQ=255)
                                                                                     caTCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCGGGGCGATCat                                                                                                                                            <  2:2419042/75‑2 (MQ=255)
                                                                                          ggTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGt                                                            <  1:1281730/150‑1 (MQ=255)
                                                                                           gTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGtt                                                           >  2:2410066/1‑150 (MQ=255)
                                                                                            tCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTc                                                          >  2:2282951/1‑150 (MQ=255)
                                                                                             cAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCg                                                         <  2:2129841/150‑1 (MQ=255)
                                                                                              aaGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGCTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCgg                                                        >  1:673539/1‑150 (MQ=255)
                                                                                                ggATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCa                                                      <  1:217994/150‑1 (MQ=255)
                                                                                                   tGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAg                                                   <  2:1992634/150‑1 (MQ=255)
                                                                                                   tGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAg                                                   <  2:1992343/150‑1 (MQ=255)
                                                                                                     aCCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGt                                                 >  1:617390/1‑150 (MQ=255)
                                                                                                       cAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTgtcgtc                                                                             <  2:1514112/120‑1 (MQ=255)
                                                                                                       cAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCTTCTTCGACCAGGAAAATGTTCGGCATAGGTGc                                               <  1:2410066/150‑1 (MQ=255)
                                                                                                         ggTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGttag                                                         >  2:1295159/1‑136 (MQ=255)
                                                                                                         ggTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCg                                                         <  1:1295159/138‑1 (MQ=255)
                                                                                                           tCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGTCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTgg                                           <  2:1049356/150‑1 (MQ=255)
                                                                                                             aGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCg                                                         <  2:94203/134‑1 (MQ=255)
                                                                                                             aGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCg                                                         >  1:94203/1‑134 (MQ=255)
                                                                                                             aGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGc                                         <  1:490118/150‑1 (MQ=255)
                                                                                                                ccTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTc                                      <  1:1579859/150‑1 (MQ=255)
                                                                                                                 cTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAg                                                                                         >  2:526737/1‑98 (MQ=255)
                                                                                                                 cTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAg                                                                                         <  1:526737/98‑1 (MQ=255)
                                                                                                                   ttGCCGACGGTAATCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTgg                                   <  1:491964/150‑1 (MQ=255)
                                                                                                                     gCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGt                                 <  1:1041122/150‑1 (MQ=255)
                                                                                                                      ccGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATa                                                    >  2:1766509/1‑130 (MQ=255)
                                                                                                                      ccGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATa                                                    <  1:1766509/130‑1 (MQ=255)
                                                                                                                        gACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGTGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGa                              >  1:1436716/1‑150 (MQ=255)
                                                                                                                        gACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGa                              >  1:700176/1‑150 (MQ=255)
                                                                                                                         aCGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGaa                             <  1:119158/150‑1 (MQ=255)
                                                                                                                         aCGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGaa                             <  1:891412/150‑1 (MQ=255)
                                                                                                                         aCGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGaa                             <  2:2162873/150‑1 (MQ=255)
                                                                                                                             tACTCGTCCAGCACATGATCGCCCCGGGCGATCACatggatgagtaccgacggcaaaggcctgacctggccatccttgacctgatgctgccgggcatcgatggcctgcagctgtgccggctgctgcgccaggagtcgcagaccctgccaa                         <  1:1864980‑M2/150‑116 (MQ=255)
                                                                                                                             tACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAGCAGGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGAAGTaa                         >  1:21864/1‑150 (MQ=255)
                                                                                                                             tACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGAAGTaa                         <  2:1159835/150‑1 (MQ=255)
                                                                                                                                tCGTCCAGCACATGATCGCCCCGGGCGATCACgtgctggacgagtaccgtcggcaaaggcctgacctggtcatccttgacctgatgctgccgggcatcgatggcctgcagctgtgccggc                                                    >  2:1312499‑M2/1‑32 (MQ=255)
                                                                                                                                tCGTCCAGCACATGATCGCCCCGGGCGATCACctggacgagtaccgtcggcaaaggcctgacctggtcatccttgacctgatgctgccgggcatcgatggcctgcagctgtgccggctgctgcgccaggagtcgcagaccctgccaatcc                      <  2:302270‑M2/150‑119 (MQ=255)
                                                                                                                                tCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGAAGTAAGcc                      >  2:1671951/1‑150 (MQ=255)
                                                                                                                                 cGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGAAGTAAGCCt                     <  1:778454/150‑1 (MQ=255)
                                                                                                                                   tCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGTAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGAAGTAAGCCtgt                   >  1:1807522/1‑150 (MQ=255)
                                                                                                                                        caggtGATCGCCCGGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGAAGTAAGCCTGTGGCGa              >  2:1568891/5‑150 (MQ=255)
                                                                                                                                        cacaTGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGAAGTAAGCCTGTGGCGa              >  2:345772/1‑150 (MQ=255)
                                                                                                                                         aggtGATCGCCCGGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGAAGTAAGCCTGTGGCGAg             >  2:44182/4‑150 (MQ=255)
                                                                                                                                         aggtGATCGCCCGGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGAAGTAAGCCTGTGGCGAg             >  1:272766/4‑150 (MQ=255)
                                                                                                                                         acaTGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGAAGTAAGCCTGTGGCGAg             >  1:1283848/1‑150 (MQ=255)
                                                                                                                                          ggtGATCGCCCGGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGAAGTAAGCCTGTGGCGAgc            >  2:432311/3‑150 (MQ=255)
                                                                                                                                           gtGATCGCCCGGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGAAGTAAGCCTGTGGCGAgcg           >  2:2214456/2‑150 (MQ=255)
                                                                                                                                           gtGATCGCCCGGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGAAGTAAGCCTGTGGCGAgcg           >  2:1642775/2‑150 (MQ=255)
                                                                                                                                           gtGATCGCCCGGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGAAGTAAGCCTGTGGCGAgcg           >  2:1806391/2‑150 (MQ=255)
                                                                                                                                                 gCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGAAGTAAGCCTGTGGCGAgcg           >  2:2280432/1‑144 (MQ=255)
                                                                                                                                                   cccGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGAAGTAAGCCTGTGGCGAGCGCTTCGCgg   <  1:106864/150‑1 (MQ=255)
                                                                                                                                                   cccGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGAAGTAAGCCTGTGGCGAGCGCTTCGCgg   <  1:105232/150‑1 (MQ=255)
                                                                                                                                                    ccGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGAAGTAACACTGTGGCGATCTCTTCGCggg  <  2:700176/150‑1 (MQ=255)
                                                                                                                                                    ccGGGCGAGCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGAAGTAAGCCTGTGGCGAGCGCTTCGCggg  <  2:92011/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGCGGGCCGTCAGCATGAGGATTGGCAGGGTCTGCGACTCCTGGCGCAGCAGCCGGCACAGCTGCAGGCCATCGATGCCCGGCAGCATCAGGTCAAGGATGACCAGGTCAGGCCTTTGCCGACGGTACTCGTCCAGCACATGATCGCCCCGGGCGATCACCTGGACATGGAAGTCGTTGCGTTGCAGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGAAGTAAGCCTGTGGCGAGCGCTTCGCGGG  >  NC_002947/3914584‑3914881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑