Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,241,041 G→C 5.6% I234M (ATC→ATG)  PP_3718 ← aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,241,0410GC 5.6% 306.7 / 6.7 100I234M (ATC→ATG) PP_3718aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (50/44);  new base C (3/3);  total (53/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

ACAGTTCCTCGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGGCGCGCGGTACGGGTTGGGGTTGGAGACGTGGTG  >  NC_002947/4240893‑4241184
                                                                                                                                                    |                                                                                                                                               
aCAGTTCCTCGCTGGCGAAGAAGGTGCTCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGa                                                                                                                                                <  2:395263/150‑1 (MQ=255)
aCAGCTCCTCGCGGGCGATGAAGGTGCCCAGGCGCCCGAACGAGGTCCCCACTTCGTCTGCGACGACCAGGATGTCGTAGGTCTGGCATAGCTGCCACATGCGCTGGAAGTAGCTCTCGTGCGGAATGATCACCCCGTCCTAGCCCATGa                                                                                                                                                <  2:1052304/150‑1 (MQ=255)
 cAGTTCCTCGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGAt                                                                                                                                               <  2:968390/150‑1 (MQ=255)
       cTCGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGAtcggctc                                                                                                                                         >  2:730934/1‑150 (MQ=255)
       cTCGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGAtcggctc                                                                                                                                         <  1:1539834/150‑1 (MQ=255)
         cGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGAtcggctcgg                                                                                                                                       <  1:442806/150‑1 (MQ=255)
           cTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCGATCAtgggctcgggc                                                                                                                                     <  1:851709/150‑3 (MQ=255)
           cTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCg                                                                                                                                     >  1:106703/1‑150 (MQ=255)
            tGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCga                                                                                                                                    >  1:260185/1‑150 (MQ=255)
                 aagaagGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGGTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAAt                                                                                                                               <  2:497553/150‑1 (MQ=255)
                 aagaagGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAAt                                                                                                                               <  1:1455415/150‑1 (MQ=255)
                   gaagGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGCTGACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATgc                                                                                                                             >  1:982645/1‑150 (MQ=255)
                    aagGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATgcc                                                                                                                            <  1:931790/150‑1 (MQ=255)
                    aagGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATgcc                                                                                                                            >  1:2191375/1‑150 (MQ=255)
                       gTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATgccgcc                                                                                                                         >  1:1764074/1‑150 (MQ=255)
                         gCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCGATCAtgggctcgggcggg                                                                                                                                  >  2:1038060/1‑134 (MQ=255)
                         gCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCGATCAtgggctcgggcggg                                                                                                                                  <  1:1038060/139‑6 (MQ=255)
                          cccAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACg                                                                                                                      >  2:2077117/1‑150 (MQ=255)
                          cccAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACg                                                                                                                      >  2:2071918/1‑150 (MQ=255)
                            cAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGtt                                                                                                                    >  2:2059272/1‑150 (MQ=255)
                               gcgcCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGTTGTAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGACACGGGGATCGGCTCGGCGAAGAATGCCGCCACGTTGTc                                                                                                                 <  1:2409633/150‑1 (MQ=255)
                               gcgcCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCTCCACGTTGTc                                                                                                                 <  1:575005/150‑1 (MQ=255)
                                    cGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCAc                                                                                                            >  1:2175091/1‑150 (MQ=255)
                                     gAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCAcc                                                                                                           >  2:2364858/1‑150 (MQ=255)
                                      aaCGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCAccc                                                                                                          >  1:636537/1‑150 (MQ=255)
                                        cGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAg                                                                                                        <  2:2191375/150‑1 (MQ=255)
                                         gAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGc                                                                                                       >  2:2381373/1‑150 (MQ=255)
                                            gTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCgag                                                                                                    >  1:17066/1‑150 (MQ=255)
                                             tCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCgaga                                                                                                   >  2:1102556/1‑150 (MQ=255)
                                             tCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCgaga                                                                                                   <  1:1102556/150‑1 (MQ=255)
                                                 cacTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGTCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGAt                                                                                               >  1:1347511/1‑150 (MQ=255)
                                                 cacTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGAt                                                                                               >  1:1189562/1‑150 (MQ=255)
                                                  acTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATc                                                                                              <  2:1288355/150‑1 (MQ=255)
                                                    ttCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCtt                                                                                            <  2:1347511/150‑1 (MQ=255)
                                                       gtcgGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTc                                                                                         <  1:730934/150‑1 (MQ=255)
                                                        tcgGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTcc                                                                                        >  1:896323/1‑150 (MQ=255)
                                                        gctgCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTcc                                                                                        <  2:519192/147‑1 (MQ=255)
                                                         cgGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCt                                                                                       <  2:896323/150‑1 (MQ=255)
                                                             gacgaACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGCTCTTGTCCTCGaa                                                                                   >  1:1081902/1‑150 (MQ=255)
                                                             gacgaACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGaa                                                                                   >  1:2285855/1‑150 (MQ=255)
                                                             gacgaACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGaa                                                                                   >  1:661069/1‑150 (MQ=255)
                                                                 aaCAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCg                                                                               <  1:2059272/150‑1 (MQ=255)
                                                                 aaCAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCg                                                                               <  1:1942122/150‑1 (MQ=255)
                                                                   cAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCTCCCGAGCCCATGATCGGCTCGGCGACGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGc                                                                             <  1:675335/150‑1 (MQ=255)
                                                                      gATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGccac                                                                          >  1:879672/1‑150 (MQ=255)
                                                                      gATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCACGAGGCCATGATCGGCTCGGCGAAGAATGCCGCCACGGTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGccac                                                                          >  2:74798/1‑150 (MQ=255)
                                                                        tGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGccacca                                                                        >  1:1424165/1‑150 (MQ=255)
                                                                          tCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAgg                                                                      >  1:84390/1‑150 (MQ=255)
                                                                            gTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAggca                                                                    >  2:1150833/1‑148 (MQ=255)
                                                                                 tCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGGCGa                                                               >  2:637038/1‑150 (MQ=255)
                                                                                   tGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAgg                                                             >  1:654648/1‑150 (MQ=255)
                                                                                   tGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAgg                                                             <  1:1503816/150‑1 (MQ=255)
                                                                                     gCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGaa                                                                    <  2:115379/141‑1 (MQ=255)
                                                                                     gCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGaa                                                                    >  1:115379/1‑141 (MQ=255)
                                                                                            tGCCACATGCGCTGTAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGcc                                                    <  1:819768/150‑1 (MQ=255)
                                                                                               cacaTGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCg                                                 >  2:2051265/1‑150 (MQ=255)
                                                                                                acaTGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGt                                                >  2:2292749/1‑150 (MQ=255)
                                                                                                acaTGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGt                                                >  1:864558/1‑150 (MQ=255)
                                                                                                acaTGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGt                                                <  1:2292749/150‑1 (MQ=255)
                                                                                                   cgcgcCGGAAGTAGCCCTCGGGCGGCATGATCACACCGCCCGAGCCCATGGTCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCa                                             <  2:982645/149‑1 (MQ=255)
                                                                                                    gcgcTGGAAGTAGCCCTCGAGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCAt                                            <  2:330305/150‑1 (MQ=255)
                                                                                                      gcTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAATCCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATgt                                          >  1:854615/1‑150 (MQ=255)
                                                                                                        tGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATCATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGCACTCGGCCACCAGGAACTCGAGGAACTCCGCCTCGTCCATgtcg                                        >  2:180043/1‑150 (MQ=255)
                                                                                                           aaGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCt                                     <  2:106703/150‑1 (MQ=255)
                                                                                                            aGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGAt                                                                                                                                               <  1:1994462/43‑1 (MQ=255)
                                                                                                            aGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGAt                                                                                                                                               >  2:1994462/1‑43 (MQ=255)
                                                                                                              tAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGgc                                  <  2:905568/150‑1 (MQ=255)
                                                                                                                gCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGccac                                                                          <  1:2112145/108‑1 (MQ=255)
                                                                                                                gCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGccac                                                                          >  2:2112145/1‑108 (MQ=255)
                                                                                                                  gcTCGGGCGGGGTGATCATTCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCGCCCGGCGAGGGGATCTGGCCTTGGAAGACGGTCACCAGGAAGTCGAGGGACACCGTCTCGTCCAGGTCGGCTGgcgcgc                              >  2:2342800/2‑150 (MQ=255)
                                                                                                                  ccTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGgcgcgc                              >  1:1607508/1‑150 (MQ=255)
                                                                                                                  ccTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGgcgcgc                              >  1:2161086/1‑150 (MQ=255)
                                                                                                                  ccTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGgcgcgc                              >  1:2235509/1‑150 (MQ=255)
                                                                                                                   cTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGa                                                               >  2:2065273/1‑116 (MQ=255)
                                                                                                                   cTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGa                                                               <  1:2065273/116‑1 (MQ=255)
                                                                                                                   cTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGGcgcgcg                             >  2:1432094/1‑150 (MQ=255)
                                                                                                                       ggCGGGATGATCACCCCGCCCGAGCCCCGGATCGGCTCGGCGGAGAATGCCGCCACGTGGTCGGCACCCAGCGGGAGGATCTTGTCCTCGGCCTCGGGCACCAGGAAGCCGACGAACTGCTGCTCGACCACAGCGTCTAGCTCGCGGTAc                         >  2:1099021/1‑150 (MQ=255)
                                                                                                                               gATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGGCGCGCGGTACgggttggg                 <  2:84390/150‑1 (MQ=255)
                                                                                                                                  cACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGGCGCGCGGTACgggttggggtt              <  2:879672/150‑1 (MQ=255)
                                                                                                                                  cACCCCGCCCGAGACCATGATCTGTTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCCGGCGCGCGGTACgggttggggtt              <  2:550439/150‑1 (MQ=255)
                                                                                                                                   aCCNCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCCAAGTCACAAAGAACTCCGCCTCGTCCACGTCGTCTGGCGCGCGGTACgggttgggggtg             >  2:1519368/1‑150 (MQ=255)
                                                                                                                                   aCCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCg                                                                               <  1:1460129/84‑1 (MQ=255)
                                                                                                                                   aCCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCg                                                                               >  2:1460129/1‑84 (MQ=255)
                                                                                                                                   aCCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGGCGCGCGGTACgggttggggttg             <  2:17066/150‑1 (MQ=255)
                                                                                                                                   aCCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGGCGCGCGGTACgggttggggttg             >  1:1314855/1‑150 (MQ=255)
                                                                                                                                     cccgcccgAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGGCGCGCGGTACGGGTTGGGGTTGga           <  2:2175091/150‑1 (MQ=255)
                                                                                                                                     cccgcccgAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGGCGCGCGGTACGGGTTGGGGTTGga           <  1:2077117/150‑1 (MQ=255)
                                                                                                                                     cccgcccgAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGGCGCGCGGTACGGGTTGGGGTTGga           <  1:2071918/150‑1 (MQ=255)
                                                                                                                                     cccgcccgAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGGCGCGCGGTACGGGTTGGGGTTGga           >  2:1475755/1‑150 (MQ=255)
                                                                                                                                      ccgcccgAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGGCGCGCGGTACgggttg                   >  1:1977640/1‑141 (MQ=255)
                                                                                                                                      ccgcccgAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGGCGCGCGGTACgggttg                   <  2:1977640/141‑1 (MQ=255)
                                                                                                                                      ccgcccgAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGGCGCGCGGTACGGGTTGGGGTTGgag          >  1:571356/1‑150 (MQ=255)
                                                                                                                                      ccgcccgAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGGCGCGCGGTACGGGTTGGGGTTGgag          >  2:2093756/1‑150 (MQ=255)
                                                                                                                                       tcgccgAGCCGATCATGGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGGCGCGCGGTa                          <  1:2009969/130‑1 (MQ=255)
                                                                                                                                       tcgccgAGCCGATCATGGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGGCGCGCGGTa                          >  2:2009969/4‑133 (MQ=255)
                                                                                                                                            gAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGGcgcgcg                             >  1:1085484/1‑125 (MQ=255)
                                                                                                                                            gAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGGcgcgcg                             <  2:1085484/125‑1 (MQ=255)
                                                                                                                                             aGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGGCGCGCGGTACGGGTTGGGGTTGGAGACgtggt   <  1:2364858/150‑1 (MQ=255)
                                                                                                                                             aGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGGCGCGCGGTACGGGTTGGGGTTGGAGACgtggt   <  2:1314855/150‑1 (MQ=255)
                                                                                                                                              gCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGGCGCGCGGTACGGGTTGGGGTTGGAGACgtggtg  <  2:2285855/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
ACAGTTCCTCGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGGCGCGCGGTACGGGTTGGGGTTGGAGACGTGGTG  >  NC_002947/4240893‑4241184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑