| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 408,431 | C→T | 10.3% | A746T (GCC→ACC) | aceE ← | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 408,431 | 0 | C | T | 10.3% | 249.8 / 15.2 | 96 | A746T (GCC→ACC) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (41/45); new base T (6/4); total (47/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01 | |||||||||||
GAGGCGATGACCGGGCCTTTGCGACCGTTCAGGCACTGCTCGACGTAGGTCTGCTGTGGCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTACATGCCCTTGATGATGCCTTCCTC > NC_002947/408283‑408575 | gagGCGATGACCGGGCCTTTGCGACCGTTCAGGCACTGCTCGACGTAGGTCTGCTGTGGCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCa < 2:168481/150‑1 (MQ=255)gagGCGATGACCGGGCCTTTGCGACCGTTCAGGCACTGCTCGACGTAGGTCTGCTGTGGCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCa > 2:1849918/1‑150 (MQ=255) ggCGCTGACAGGGCCTTTGCGACCGTTCAGGCACTGCTCGACGTAGGTCTGCTGTGGCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCAcc > 2:98958/1‑150 (MQ=255) ggCGATGACCGGGCCTTTGCGACCGTTCAGGCACTGATCGTCGTAGGTCTGCTGTGGCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCAcc < 1:2343167/150‑1 (MQ=255) gCGATGACCGGGCCTTTGCGACCGTTCAGGCACTGCTCGACGTAGGTCTGCTGTGGCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCg > 2:2376717/1‑150 (MQ=255) gCGATGACCGGGCCTTTGCGACCGTTCAGGCACTGCTCGACGTAGGTCTGCTGTGGCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGATCCAAATGTCGGTAcca > 2:1957118/1‑149 (MQ=255) gATGACCGGGCCTTTGCGACCGTTCAGGCACTGCTCGACGTAGGTCTGCTGTGGCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGAc < 2:769347/150‑1 (MQ=255) gACCGGGCCTTTGCGACCGTTCAGGCACTGCTCGACGTAGGTCTGCTGTGGCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGtt < 2:1644423/150‑1 (MQ=255) cGGGCCTTTGCGACCGTTCAGGCACTGCTCGACGTAGGTCTGCTGTGGCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGTGCCGACGtgt < 1:881562/148‑3 (MQ=255) cGGGCCTTTGCGACCGTTCAGGCACTGCTCGACGTAGGTCTGCTGTGGCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGTGCCGACGtgt > 2:881562/1‑146 (MQ=255) cGGGCCTTTGCGACCGTTCAGGCACTGCTCGACGTAGGTCTGCTGTGGCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGaa < 2:717763/150‑1 (MQ=255) cGGGCCTTTGCGACCGTTCAGGCACTGCTCGACGTAGGTCTGCTGTGGCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGaa > 2:384280/1‑150 (MQ=255) cGGGCCTTTGCGACCGTTCAGGCACTGCTCGACGTAGGTCTGCTGTGGCTTCTGGCCAGGCTTCAGGCGGTTGGAGCGTGCTACGGCCGCGCCGGCGCTACGCAGTTCGTTGAAGCAGGTGACGCTTCACACGTCGGCACCGACGTTGaa > 1:644708/1‑150 (MQ=255) gggCCTTTGCGACCGTTCAGGCACTGCTCGACGTAGGTCTGCTGTGGCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAAc > 2:1552962/1‑150 (MQ=255) cTTTGCGACCGTTCAGGCACTGCTCGACGTAGGTCTGCTGTGGCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCtt < 2:2423493/150‑1 (MQ=255) tttGCGACCGTTCAGGCACTGCTCGACGTAGGTCTGCTGTGGCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGTCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTc > 1:612511/1‑150 (MQ=255) tttGCGACCGTTCAGGCACTGCTCGACGTAGGTCTGCTGTGGCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTc > 2:1481999/1‑150 (MQ=255) gACCGTTCAGGCACTGCTCGACGTAGGTCTGCTGTGGCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCa < 1:2046986/150‑1 (MQ=255) gACCGTTCAGGCACTGCTCGACGTAGGTCTGCTGTGGCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCa < 1:2047208/150‑1 (MQ=255) gACCGTTCAGGCACTGCTCGACGTAGGTCTGCTGTGGCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCa < 2:1952808/150‑1 (MQ=255) gACCGTTCAGGCACTGCTCGACGTAGGTCTGCTGTGGCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCa > 1:1423155/1‑150 (MQ=255) aCCGTTCAGGCACTGCTCGACGTAGGTCTGCTGTGGCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCCCCACACGTCGGCACCGACGTTGAACTCTTCACGCAg > 2:1125267/1‑150 (MQ=255) ccGTTCAGGCACTGCTCGACGTAGGTCTGCTGTGGCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAgg < 2:1334262/150‑1 (MQ=255) ccGTTCAGGCACTGCTCGACGTAGGTCTGCTGTGGCTTCTGGCCAGGCTGCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAgg < 2:1832262/150‑1 (MQ=255) aGGCACTGCTCGACGTAGGTCTGCTGTGGCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATcttc < 2:1671449/150‑1 (MQ=255) aCTGCTCGACGTAGGTCTGCTGTGGCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATcttcgcgg > 1:133149/1‑150 (MQ=255) aCTGCTCGACGTAGGTCTGCTGTGGCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATcttc > 2:916819/1‑146 (MQ=255) aCTGCTCGACGTAGGTCTGCTGTGGCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATcttc < 1:916819/146‑1 (MQ=255) tGCTCGACGTAGGTCTGCTGTGGCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATcttcgcggct > 2:438993/1‑150 (MQ=255) ggcGTAGGTCTGCTGTGGCTTCTGGCCATGCTTTAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCCGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATcttcgcggcttcgcg < 2:1234171/148‑1 (MQ=255) gACGTAGGTCTGCTGTGGCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGTCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATcttcgcggcttcgcg < 2:912242/150‑1 (MQ=255) aCGTAGGTCTGCTGTGGCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTGGAAGCTGGGGACGCTCCACACGTCAGCACCGACGTTGAACTCTTCACGCAGGATcttcgcggcttcgcgg > 2:458933/1‑150 (MQ=255) tCTGCTGTGGCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGTTCTTCGCGGCTTCGCGGACTTcgc > 1:2296181/1‑150 (MQ=255) tCTGCTGTGGCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTcgc > 1:1398763/1‑150 (MQ=255) gtgGCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGa > 2:2287949/1‑150 (MQ=255) ggCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATg < 2:133149/150‑1 (MQ=255) cTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGcc < 2:1423155/150‑1 (MQ=255) ccAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAg < 1:384280/150‑1 (MQ=255) ccAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAg < 2:2148193/150‑1 (MQ=255) cAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGc > 2:1491490/1‑150 (MQ=255) cAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGc < 2:644708/150‑1 (MQ=255) cAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACAAGTGGGCACCGACGTGGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGc > 1:883533/1‑150 (MQ=255) gCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCa > 2:1570426/1‑150 (MQ=255) cTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCAt < 1:2316594/150‑1 (MQ=255) aGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCCGGATGGTGCCGGAGccttacaaa > 2:734282/1‑143 (MQ=255) aGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGc > 2:1343376/1‑150 (MQ=255) gcgcGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACgtgg > 1:2027514/1‑150 (MQ=255) cgcgTTCCACGTCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACgtggt < 2:612511/150‑1 (MQ=255) cgTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGgtgt < 1:1957118/150‑1 (MQ=255) gTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAActct > 1:1668929/1‑77 (MQ=255) gTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAActct < 2:1668929/77‑1 (MQ=255) gcgaCGGCCTGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCg > 2:477932/4‑150 (MQ=255) ccACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATcttcgcggcttcgcg < 1:2098444/100‑1 (MQ=255) ccACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATcttcgcggcttcgcg > 2:2098444/1‑100 (MQ=255) cACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGc > 1:1313479/1‑150 (MQ=255) aCGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGc < 2:1369410/146‑1 (MQ=255) aCGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGc > 1:1369410/1‑146 (MQ=255) ccAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTcgc > 2:644066/1‑150 (MQ=255) aGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCATTTTCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCgg < 1:2376717/150‑1 (MQ=255) ggCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTcgcg > 2:2126282/1‑148 (MQ=255) ggCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTcgcg < 1:2126282/148‑1 (MQ=255) ggCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCggg < 2:1116935/150‑1 (MQ=255) ggcgccgcgACGCAGTTCGTGGAAGCTGGTGACGATCCACACGTCGGCACGGACGTTGAACTCTTCACGCAGGAGCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGgt < 2:1161770/145‑1 (MQ=255) cGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGgtgt > 2:2387404/1‑150 (MQ=255) gcgACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGc > 1:1301543/1‑132 (MQ=255) gcgACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGCTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGc < 2:1301543/132‑1 (MQ=255) gACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGGTGTCCTCtt > 2:200325/1‑150 (MQ=255) ttCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTGCGGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTCcagcag > 1:1333998/1‑150 (MQ=255) tCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTCCAGCAgg < 1:1481999/150‑1 (MQ=255) gTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTa > 1:2155193/1‑150 (MQ=255) tGACGCTCCACGCGTCGGCGCCGACGTTGATCTCTTCACGCAGGATGTTCGCGGCTTCGCGGACGTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTACATGCCCTtga < 2:749585/150‑1 (MQ=255) tGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTGAAGCAGGTACATggcttgta < 2:524773/150‑8 (MQ=255) tGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTACATGCCCTtga < 1:502554/150‑1 (MQ=255) gACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTACATGCCCTtgat > 2:2077799/1‑150 (MQ=255) gACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTACATGCCCTtgat < 1:1491490/150‑1 (MQ=255) gCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTACATGCCCTTgatgat > 1:1512669/1‑150 (MQ=255) gCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTACATGCCCTTgatgat < 1:1858670/150‑1 (MQ=255) cTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTACATGCCCTTGatgatg < 1:70569/150‑1 (MQ=255) tCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGAGGCTCACGCGCGGTATGGCGCCGAAGACCATCAGCTGTACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTACATGCCCTTGATGATGc < 1:2387404/150‑1 (MQ=255) ttcaacGTCGGTGCCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGgt > 1:1185442/5‑114 (MQ=255) ttcaacGTCGGTGCCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGgt < 2:1185442/110‑1 (MQ=255) ccACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGAATATTTCGCTGAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTACATGCCCTTGATGATGcc < 2:323296/150‑1 (MQ=255) caacGTCGGTGCCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGgtgt < 2:1319436/112‑1 (MQ=255) caacGTCGGTGCCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGgtgt > 1:1319436/3‑114 (MQ=255) caacGTCGGTGCCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTACATGCCCTTGATGATGCCtt > 1:2418442/3‑150 (MQ=255) cacGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGCTCGTCTGCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTACATGCCCTTGATGATGCCTTc < 1:644066/150‑1 (MQ=255) aacGTCGGTGCCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCAt > 2:2340545/2‑81 (MQ=255) aacGTCGGTGCCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCAt < 1:2340545/80‑1 (MQ=255) acGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTACATGCCCTTGATGATGCCTTcc < 2:1398763/150‑1 (MQ=255) cGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGgtgt < 1:1904503/95‑1 (MQ=255) cGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGgtgt > 2:1904503/1‑95 (MQ=255) gTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTACATGCCCTTGATGATGCCTTCCTc < 1:2077799/150‑1 (MQ=255) gTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTACATGCCCTTGATGATGCCTTCCTc > 1:802825/1‑150 (MQ=255) gTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTACATGCCCTTGATGATGCCTTCCTc > 2:681918/1‑150 (MQ=255) gCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTcgc < 1:2381258/100‑1 (MQ=255) gCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTcgc > 2:2381258/1‑100 (MQ=255) | GAGGCGATGACCGGGCCTTTGCGACCGTTCAGGCACTGCTCGACGTAGGTCTGCTGTGGCTTCTGGCCAGGCTTCAGGCGGTTGGCGCGTTCCACGGCCAGGCCGTCGCGACGCAGTTCGTTGAAGCTGGTGACGCTCCACACGTCGGCACCGACGTTGAACTCTTCACGCAGGATCTTCGCGGCTTCGCGGACTTCGCGCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTACATGCCCTTGATGATGCCTTCCTC > NC_002947/408283‑408575 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |