BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,902,717	T→C	7.5%	V196A (GTG→GCG) ‡	PP_4310 →	hydantoin racemase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,902,717	0	T	C	7.5%	330.8 / 11.2	116	V196A (GTG→GCG) ‡	PP_4310	hydantoin racemase
Reads supporting (aligned to +/- strand): ref base T (48/58); new base C (3/6); total (52/64)
Fisher's exact test for biased strand distribution p-value = 7.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

Only 100 of 119 total aligned reads displayed.

GCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGTGGTGGGCTGGCCGATGCGCTTCGGCCGCTAGAGATCC  >  NC_002947/4902568‑4902865

gCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGt                                                                                                                                                      <  1:301147/150‑1 (MQ=255)

  tggaactggaaGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACACGGTCCTATTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGc                                                                                                                                                    >  1:1614254/1‑150 (MQ=255)

       ctggaaGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCggtg                                                                                                                                               <  1:933107/150‑1 (MQ=255)

          gaaGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGgtggtg                                                                                                                                            <  1:295659/150‑1 (MQ=255)

           aaGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCACGCCggtgcgtt                                                                                                                                           <  2:2111097/150‑5 (MQ=255)

           aaGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCACGCCggtgcgtt                                                                                                                                           <  2:2361157/150‑5 (MQ=255)

            aGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGa                                                                                                                                          <  2:1476654/150‑1 (MQ=255)

            aGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCACGCCggtgcgttg                                                                                                                                          <  1:316213/150‑6 (MQ=255)

             cacggcccGCAGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCAGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGtgggggga                                                                                                                                         >  2:1557763/6‑148 (MQ=255)

              ccGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGgtggtg                                                                                                                                            <  1:2391000/146‑1 (MQ=255)

              ccGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGgtggtg                                                                                                                                            >  2:2391000/1‑146 (MQ=255)

                ggcccGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGgt                                                                                                                                      <  1:1531370/148‑1 (MQ=255)

                gACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGAGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGgt                                                                                                                                      >  2:1217541/1‑150 (MQ=255)

                  cccGCTGCGGGCCGGGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTCCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGCGCAGATTCGCCAACGCACCGGCGTGCCGGTTGTGGATGgtgt                                                                                                                                    <  2:890091/150‑1 (MQ=255)

                      cTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGt                                                                                                                                >  1:970994/1‑150 (MQ=255)

                           ggCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGc                                                                                                                           >  1:816527/1‑150 (MQ=255)

                           ggCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGc                                                                                                                           <  1:540836/150‑1 (MQ=255)

                              cGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGCGCTTGTGGTGGATGGTGTGAGTGCAGCGGt                                                                                                                        <  1:63150/150‑1 (MQ=255)

                               gTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTg                                                                                                                       >  2:1955130/1‑150 (MQ=255)

                                 ggAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGGGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGgt                                                                                                                                      >  2:1322585/1‑133 (MQ=255)

                                 ggAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGgt                                                                                                                                      <  1:1322585/133‑1 (MQ=255)

                                 ggAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGACGGTGTGAGTGCAGCGGTGAc                                                                                                                     <  1:1211792/150‑1 (MQ=255)

                                    ggCGCTTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCAGAGGACGGCGGCCGGGGGTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCCCCGGCGGTCCTGGTGTGGACGGTGTGAGAGCCGCGGTGGcccg                                                                                                                  >  2:928271/1‑148 (MQ=255)

                                       gATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACTGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGc                                                                                                               <  1:50284/150‑1 (MQ=255)

                                       gATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGAc                                                                                                                     <  1:110023/144‑1 (MQ=255)

                                       gATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGAc                                                                                                                     >  2:110023/1‑144 (MQ=255)

                                       gATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGc                                                                                                               >  1:1931056/1‑150 (MQ=255)

                                        aTTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGgtgt                                                                                                                                    >  2:1129468/1‑128 (MQ=255)

                                        aTTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGgtgt                                                                                                                                    <  1:1129468/128‑1 (MQ=255)

                                            tCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGa                                                                                                                                  <  1:263519/126‑1 (MQ=255)

                                            tCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGa                                                                                                                                  >  2:263519/1‑126 (MQ=255)

                                             cGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGTCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATc                                                                                                         <  2:917708/150‑1 (MQ=255)

                                                gCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCt                                                                                                      <  2:619298/150‑1 (MQ=255)

                                                  aGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGAAACCCCGAGCAAATCGCGTTTATTCGCCAGTTGGCCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTgg                                                                                                    <  1:2121533/150‑1 (MQ=255)

                                                    gCCNAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTg                                                                                                  >  2:1503877/1‑150 (MQ=255)

                                                    gCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTg                                                                                                  >  2:2379798/1‑150 (MQ=255)

                                                      cGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTg                                                                                                     <  1:325586/145‑1 (MQ=255)

                                                      cGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTg                                                                                                     >  2:325586/1‑145 (MQ=255)

                                                       gAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGGgcgc                                                                                               >  1:119675/1‑150 (MQ=255)

                                                        aaCGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCa                                                                                              <  1:1503877/150‑1 (MQ=255)

                                                        aaCGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCa                                                                                              >  2:1355859/1‑150 (MQ=255)

                                                           gcgcCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATgg                                                                                           <  2:2107832/150‑1 (MQ=255)

                                                           gcgcCGGGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGTGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATgg                                                                                           <  1:2091421/150‑1 (MQ=255)

                                                                 tGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCt                                                                                                      >  1:409139/1‑133 (MQ=255)

                                                                 tGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCt                                                                                                      <  2:409139/133‑1 (MQ=255)

                                                                 tGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGa                                                                                     <  2:1931056/150‑1 (MQ=255)

                                                                 tGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGa                                                                                     <  2:816527/150‑1 (MQ=255)

                                                                    gcgACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCa                                                                                  >  2:1282790/1‑150 (MQ=255)

                                                                     cgacgaCAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCAc                                                                                 <  1:2367023/150‑1 (MQ=255)

                                                                     cgacgaCAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCAc                                                                                 >  2:841430/1‑150 (MQ=255)

                                                                      gacgacAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACg                                                                                >  2:2147654/1‑150 (MQ=255)

                                                                      gacgacAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACg                                                                                >  1:2440790/1‑150 (MQ=255)

                                                                          acAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCa                                                                            >  1:1031474/1‑150 (MQ=255)

                                                                           cAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCaa                                                                           >  1:860104/1‑150 (MQ=255)

                                                                            aaGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATg                                                                                                                                        >  2:1092394/1‑88 (MQ=255)

                                                                            aaGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATg                                                                                                                                        <  1:1092394/88‑1 (MQ=255)

                                                                            aaGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCaaa                                                                          <  1:1063274/150‑1 (MQ=255)

                                                                            aaGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCaaa                                                                          <  2:1652156/150‑1 (MQ=255)

                                                                            aaGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCaaa                                                                          <  1:1930215/150‑1 (MQ=255)

                                                                                  gAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTgcgc                                                                    <  2:1701676/150‑1 (MQ=255)

                                                                                     gTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCAcc                                                                 <  1:1591157/150‑1 (MQ=255)

                                                                                        aTCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTAt                                                              <  1:1738415/150‑1 (MQ=255)

                                                                                          cTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCa                                                                                                                             >  1:454268/1‑85 (MQ=255)

                                                                                          cTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGc                                                            <  1:829802/150‑1 (MQ=255)

                                                                                            gCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGa                                                          <  2:886456/150‑1 (MQ=255)

                                                                                            gCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGa                                                          <  1:2242418/150‑1 (MQ=255)

                                                                                             ccTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGAc                                                         >  2:590339/1‑150 (MQ=255)

                                                                                              cTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTAt                                                              <  1:1640025/144‑1 (MQ=255)

                                                                                              cTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTAt                                                              >  2:1640025/1‑144 (MQ=255)

                                                                                              cTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACg                                                        >  2:516619/1‑150 (MQ=255)

                                                                                                gggTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCggtggt                                                                                                                                             >  2:2276073/1‑63 (MQ=255)

                                                                                                gggTTGTGGCGGCATGGCCGGGCTGGACGAGAACATTCGCCAACGCACCGGCGTGCCggtggt                                                                                                                                             <  1:2276073/63‑1 (MQ=255)

                                                                                                     gtgGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACggg                                                 <  1:1217541/150‑1 (MQ=255)

                                                                                                           gcATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCgaaga                                           >  1:309766/1‑150 (MQ=255)

                                                                                                            cATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGACAGCGCAAGGTGCGCACCTATGCGACGCCACGGGCgaagaa                                          >  1:723327/1‑150 (MQ=255)

                                                                                                                  cGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAgtggt                                    <  2:970994/150‑1 (MQ=255)

                                                                                                                    ggCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGtggtgg                                  <  2:604403/150‑1 (MQ=255)

                                                                                                                        ggACGAGCAGATTCGCCAACGCACCGGCGTGCACGGGTTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGCATCGCTGTTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGTGGTGGGCTg                              >  1:789895/1‑150 (MQ=255)

                                                                                                                         gACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGTGGTGGGCTgg                             >  1:979718/1‑150 (MQ=255)

                                                                                                                            gAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGTGGTGGGCTGGCCg                          >  1:2282244/1‑150 (MQ=255)

                                                                                                                            gAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGTGGTGGGCTGGCCg                          <  1:1940805/150‑1 (MQ=255)

                                                                                                                               cAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTg                                                                                                     >  1:689514/1‑72 (MQ=255)

                                                                                                                               cAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTg                                                                                                     <  2:689514/72‑1 (MQ=255)

                                                                                                                                      gCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGTGGTGGGCTGGCCGATGCGCTTCg                >  2:631424/1‑150 (MQ=255)

                                                                                                                                      catccaccACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGTGGTGGGCTGGCCGAt                        >  2:1124589/8‑142 (MQ=255)

                                                                                                                                      catccaccACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGTGGTGGGCTGGCCGAt                        <  1:1124589/135‑1 (MQ=255)

                                                                                                                                       ctaaCGCACCGTCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGTGGTGGGCTGGCCGATGCGCTTCgg               <  1:1913436/148‑1 (MQ=255)

                                                                                                                                       ccAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGTGGTGGGCTGGCCGATGCGCTTCgg               >  2:1112535/1‑150 (MQ=255)

                                                                                                                                       ccAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGTGGTGGGCTGGCCGATGCGCTTCgg               >  2:2276836/1‑150 (MQ=255)

                                                                                                                                         aaCGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGTGGTGGGCTGGCCGATGCGCTTCGGcc             >  2:1287150/1‑150 (MQ=255)

                                                                                                                                          caccACCGGCACGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGTGGTGGGCTGGCCGATGCGCTTCgagat            >  1:1881387/4‑146 (MQ=255)

                                                                                                                                           accACCGGCACGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGTGGTGGGCTGGCCGATGCGCTTCGGCCGc           >  1:1374634/3‑150 (MQ=255)

                                                                                                                                            ccACCGGCACGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGTGGTGGGCTGGCCGATgcgc                    >  1:823589/2‑140 (MQ=255)

                                                                                                                                            ccACCGGCACGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGTGGTGGGCTGGCCGATgcgc                    <  2:823589/139‑1 (MQ=255)

                                                                                                                                             aaCCGGCGTGCCGGTGGTGGATGGTGTGAGTGGAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGTGGTGGGCTGGCCGATGCGCTTCGGCCGCTa         <  2:1013546/149‑1 (MQ=255)

                                                                                                                                               ccGNCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGCTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGCGGGGGCCTGGCCGATGCGCTTCGGCCGCTaga       >  2:1496985/1‑150 (MQ=255)

                                                                                                                                                cGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGTGGTGGGCTGGCCGATGCGCTTCGGCCGCTagag      >  2:1547767/1‑150 (MQ=255)

                                                                                                                                                    gTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCgaag                                            >  1:2067471/1‑108 (MQ=255)

                                                                                                                                                    gTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGTGGTGGGCTGGCCGATGCGCTTCGGCCGCTAGAGATcc  <  2:860104/150‑1 (MQ=255)

                                                                                                                                                     tGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCAc                                                                                 <  2:1400245/70‑1 (MQ=255)

GCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGTGGTGGGCTGGCCGATGCGCTTCGGCCGCTAGAGATCC  >  NC_002947/4902568‑4902865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`