Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,914,274	G→T	7.2%	P157Q (CCG→CAG)	ccmF ←	holocytochrome c synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,914,274	0	G	T	7.2%	305.5 / 7.1	97	P157Q (CCG→CAG)	ccmF	holocytochrome c synthetase
Reads supporting (aligned to +/- strand):  ref base G (38/52);  new base T (3/4);  total (41/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

CGTCCAGCCGGCCGCCGAGCAAGGCGGCAATGGCGAACGCGAAAGCCACCGAGAAGCCCACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCAGCACGCGGGCCAGCATCACCTGCGGCAACTGCCGGGAGAACACCGACACGGCAAAGGTCCAGCCGCC  >  NC_002947/4914125‑4914419                                                                                                                                                      |                                                                                                                                                   cGTCCAGCCGGCCGCCGAGCAAGGCGGCAATGGCGAACGCGAAAGCCACCGAGAAGCCCACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCg                                                                                                                                                    <  2:2190280/150‑1 (MQ=255)      aGCCGCCCGCCGAGCAAGGCGGAAATAGAGACCACGAAAGGCACCGAGAAGCCCACATAGCCCATGTAGAGCATGGGTGGGGGAACGATCAGGCCAAAGTCCTGAAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGgcagc                                                                                                                                               <  2:981314/150‑1 (MQ=255)        ccggccgCCGAGCAAGGCGGCAATGGCGAACGCGAAAGCCACCGAGAAGCCCACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGcagcag                                                                                                                                             <  1:1815053/150‑1 (MQ=255)        ccggccgCCGAGCAAGGCGGCAATGGCGAACGCGAAAGCCACCGAGAAGCCCACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGcagcag                                                                                                                                             <  1:1539737/150‑1 (MQ=255)          ggccgCCGAGCAAGGCGGCAATGGCGAACGCGAAAGCCACCGAGAAGCCCACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGgc                                                                                                                                           >  1:937396/1‑150 (MQ=255)            ccgccgAGCAAGGCGGCAATGGCGAACGCGAAAGCCACCGAGAAGCCCACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCTGCCGCAGgt‑gc                                                                                                                                        <  1:661284/150‑1 (MQ=255)            ccgccgAGCAAGGCGGCAATGGCGAACGCGAAAGCCACCGAGAAGCCCACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCTGCCGCAGgt‑gc                                                                                                                                        <  1:1681504/150‑1 (MQ=255)             cgccgAGCAAGGCGGCAATGGCGAACGCGAAAGCCACCGAGAAGCCCACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCt                                                                                                                                       >  1:2448599/1‑150 (MQ=255)              gccgAGCAAGGCGGCAATGGCGAACGCGAAAGCCACCGAGAAGCCCACATAGCCCATGGAGAGCATGGGCAGGTGAAGGATCAGGCCAAAGTCCTGCAACAGAGGGTTGAGGTCACGGCCATCGGTCGGCACGTGCGgcaacacgggccg                                                                                                                                       >  2:1113327/1‑143 (MQ=255)               ccgAGCAAGGCGGCAATGGCGAACGCGAAAGCCACCGAGAAGCCCACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCAGCGCCTGCTGCCGCAggtgccga                                                                                                                                      <  2:388917/150‑7 (MQ=255)                 gAGCAAGGCGGCAATGGCGAACGCGAAAGCCACCGAGAAGCCCACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGaa                                                                                                                                   <  1:432718/150‑1 (MQ=255)                  aGCAAGGCGGCAATGGCGAACGCGAAAGCCACCGAGAAGCCCACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAAc                                                                                                                                  <  1:825515/150‑1 (MQ=255)                    cAAGGCGGCAATGGCGAACGCGAAAGCCACCGAGAAGCCCACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACgg                                                                                                                                <  2:1609114/150‑1 (MQ=255)                           gcAATGGCGAACGCGAAAGCCACCGAGAAGCCCACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGaa                                                                                                                         <  1:369907/150‑1 (MQ=255)                           gcAATGGCGAACGCGAAAGCCACCGAGAAGCCCACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGaa                                                                                                                         <  1:1489805/150‑1 (MQ=255)                              aTGGCGAACGCGAAAGCCACCGAGAAGCCCACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTa                                                                                                                      >  1:1318661/1‑150 (MQ=255)                               tGGCGAACGCGAAAGCCACCGAGAAGCCCACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTaa                                                                                                                     <  1:1622809/150‑1 (MQ=255)                               tGGCGAACGCGAAAGCCACCGAGAAGCCCACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTaa                                                                                                                     <  1:1813627/150‑1 (MQ=255)                               tGGCGAACGCGAAAGCCACCGAGAAGCCCACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTaa                                                                                                                     <  1:916018/150‑1 (MQ=255)                                 gCGAACGCGAAAGCCACCGAGAAGCCCACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATg                                                                                                                   <  1:1155106/150‑1 (MQ=255)                                   gAACGCGAAAGCCACCGAGAAGCCCACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGAt                                                                                                                 >  1:1606591/1‑150 (MQ=255)                                     aCGCGAAAGCCACCGAGAAGCCCACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGAtca                                                                                                               <  1:272685/150‑1 (MQ=255)                                           aaGCCACCGAGAAGCCCACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGtt                                                                                                                             <  2:221452/130‑1 (MQ=255)                                           aaGCCACCGAGAAGCCCACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGtt                                                                                                                             >  1:221452/1‑130 (MQ=255)                                               cACCGAGAAGCCCACATAGCGCATGTAGAGCATGGGCGGATGAACGATACGGCGGAAGTCCTGCAACAGCGGGGTGAGGTAACGGCCATCGGTCGGCACCTACGGCAGCAGGC‑GCTGGAACGGGTGGGAAGTAATGAtcaggacgctcag                                                                                                     >  2:334409/1‑150 (MQ=255)                                               cACCGAGAAGCCCACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGtt                                                                                                                             <  1:403455/126‑1 (MQ=255)                                               cACCGAGAAGCCCACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGtt                                                                                                                             >  2:403455/1‑126 (MQ=255)                                               cACCGAGAAGCCCACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAAt                                                                                                                    <  2:2331352/135‑1 (MQ=255)                                               cACCGAGAAGCCCACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAAt                                                                                                                    >  1:2331352/1‑135 (MQ=255)                                               cACCGAGAAGCCCACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGAtcaggaagctcag                                                                                                     >  2:1648330/1‑150 (MQ=255)                                                  cGAGAAGCCCACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATGGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGAtcaggaagctcaggaa                                                                                                  >  2:1213625/1‑150 (MQ=255)                                                   gagaAGCCCACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGAtcaggaagctcaggaag                                                                                                 <  2:214770/150‑1 (MQ=255)                                                        gCCCACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCCCACCACGGGCGATGTTGTA‑CATGCGGCCGTAACGGGTTGGAAGTAATGATCAGGAAGCTCAGTAAGCCCAc                                                                                            >  1:876461/1‑150 (MQ=255)                                                        gCCCACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCGGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCAc                                                                                            >  2:546758/1‑150 (MQ=255)                                                         cccACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAAc                                                                                                                                  <  1:2211837/111‑1 (MQ=255)                                                         cccACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAAc                                                                                                                                  >  2:2211837/1‑111 (MQ=255)                                                          ccATATAGCCGATGTAGAGCATGGGTGGGTGAACGACCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGc                                                                                          >  2:1008769/1‑150 (MQ=255)                                                          ccACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCGCAGGAGGCCCACGc                                                                                          >  1:1696490/1‑150 (MQ=255)                                                           cacaTAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGTAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCt                                                                                         >  2:1863903/1‑150 (MQ=255)                                                             caTAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCGCAGGAAGCCCACGCTGa                                                                                       <  2:937396/150‑1 (MQ=255)                                                              aTAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGAt                                                                                      >  2:1721356/1‑150 (MQ=255)                                                              aTAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGAt                                                                                      <  1:932694/150‑1 (MQ=255)                                                                aGCCCATGTACAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGAtcagga                                                                                                            <  2:1805745/126‑1 (MQ=255)                                                                aGCCCATGTACAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGAtcagga                                                                                                            >  1:1805745/1‑126 (MQ=255)                                                                 gCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCAt                                                                                   >  1:2303533/1‑150 (MQ=255)                                                                 gCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCAt                                                                                   <  1:1213625/150‑1 (MQ=255)                                                                   ccATGTAGAGCATGGGTGGGTGACCGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGc                                                                                 <  1:1113327/150‑1 (MQ=255)                                                                   ccATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCTGCCGCAggtgcc                                                                                                                                        >  2:1318072/1‑92 (MQ=255)                                                                   ccATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCTGCCGCAGgt‑gcc                                                                                                                                       <  1:1318072/96‑2 (MQ=255)                                                                   ccATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGc                                                                                 >  1:2070071/1‑150 (MQ=255)                                                                   ccATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGc                                                                                 <  1:1721356/150‑1 (MQ=255)                                                                   ccATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGc                                                                                 >  2:468665/1‑150 (MQ=255)                                                                   ccATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGc                                                                                 <  1:334409/150‑1 (MQ=255)                                                                    cATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGcc                                                                                <  1:2106166/150‑1 (MQ=255)                                                                    cATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGcc                                                                                <  2:1375666/150‑1 (MQ=255)                                                                          ctacATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTAc                                                                          <  1:712862/147‑1 (MQ=255)                                                                               tgggtgggTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCa                                                                     <  2:1870754/150‑1 (MQ=255)                                                                               tgggtgggTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCa                                                                     <  1:1653941/150‑1 (MQ=255)                                                                                  gtgggtgAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCAGCa                                                                  >  2:65687/1‑150 (MQ=255)                                                                                    gggtgAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACggg                                                                                                                               <  1:1470862/87‑1 (MQ=255)                                                                                    gggtgAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACggg                                                                                                                               >  2:1470862/1‑87 (MQ=255)                                                                                     ggtgAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCAGCAcgc                                                               <  2:2303533/150‑1 (MQ=255)                                                                                       tgAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACgg                                                                                                                                <  1:188510/83‑1 (MQ=255)                                                                                       tgAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACgg                                                                                                                                >  2:188510/1‑83 (MQ=255)                                                                                       tgAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCAGCACGCgg                                                             <  1:978926/150‑1 (MQ=255)                                                                                       tgAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCAGCACGCgg                                                             <  1:1285683/150‑1 (MQ=255)                                                                                            gATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCAGCACGCGGGCCAg                                                        <  2:2070071/150‑1 (MQ=255)                                                                                                  gCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGtt                                                                                                                             >  1:242366/1‑75 (MQ=255)                                                                                                  gCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGtt                                                                                                                             <  2:242366/75‑1 (MQ=255)                                                                                                  gCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCAGCACGCGGGCCAGCATCAc                                                  >  1:1385043/1‑150 (MQ=255)                                                                                                      aaGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCAGCACGCGGGCCAGCATCACCTGc                                              <  2:593750/150‑1 (MQ=255)                                                                                                        gTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCAGCACGCGAGCCAGCATCACCTGCgg                                            >  1:739590/1‑150 (MQ=255)                                                                                                           cTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCGGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCAGCACGCGGGCCAGCATCACCTGCGGCaa                                         <  1:546758/150‑1 (MQ=255)                                                                                                           cTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCAGCACGCGGGCCAGCATCACCTGCGGCaa                                         <  2:421425/150‑1 (MQ=255)                                                                                                            tGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCAGCACGCGGGCCAGCATCACCTGCGGCAAc                                        >  2:1674135/1‑150 (MQ=255)                                                                                                             gCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCAGCACGCGGGCCAGCATCACCTGCGGCAACt                                       <  1:1674135/150‑1 (MQ=255)                                                                                                                aCAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCAGCACGCGGGCCAGCATCACCTGCGGCAACTGcc                                    >  2:2274410/1‑150 (MQ=255)                                                                                                                 cAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCAGCACGCGGGCCAGCATCACCTGCGGCAACTGCCg                                   >  2:1089296/1‑150 (MQ=255)                                                                                                                  aGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGAAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCAGCACGCGGGCCAGCATCACCTGCGGCAACTGCCgg                                  <  1:1008769/150‑1 (MQ=255)                                                                                                                    cGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCAGCACGCGGGCCAGCATCACCTGCGGCAACTGCCGGga                                <  2:2448599/150‑1 (MQ=255)                                                                                                                            ggTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCAGCACGCGGGCCAGCATCACCTGCGGCAACTGCCGGga                                >  2:432517/1‑142 (MQ=255)                                                                                                                            ggTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCAGCACGCGGGCCAGCATCACCTGCGGCAACTGCCGGga                                <  1:432517/142‑1 (MQ=255)                                                                                                                                 cGGCCATCGGTCGGAACCTGCGGCCGACGGA‑TCGCCATCGGCGTGGAAGCAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCAGCACGCGGGCCAGCATCACCTGCGGCAACTGCCGGGAGAACACCGACACg                   <  2:1841272/150‑1 (MQ=255)                                                                                                                                  ggCNATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCAGCACGCGGGCCAGCATCACCTGCGGCAACTGCCGGGAGAACACCGACACgg                  >  2:1515067/1‑150 (MQ=255)                                                                                                                                    ccATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGTAGCCCACGCTGATCATGCCCATTACCGCCAGCACGCGGGCCAGCATCACATGCGGCAACTGCACAGAGAACACCGACACGGCa                >  1:1307064/1‑150 (MQ=255)                                                                                                                                      aTCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTaa                                                                                                                     <  1:1790560/47‑1 (MQ=255)                                                                                                                                      aTCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTaa                                                                                                                     >  2:1790560/1‑47 (MQ=255)                                                                                                                                       tcggtcggCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCAGCACGCGGGCCAGCATCACCTGCGGCAACTGCCGGGAGAACACCGACACg                   >  2:217485/1‑144 (MQ=255)                                                                                                                                       tcggtcggCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCAGCACGCGGGCCAGCATCACCTGCGGCAACTGCCGGGAGAACACCGACACg                   <  1:217485/144‑1 (MQ=255)                                                                                                                                       tcggtcggCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCAGCACGCGGGCCAGCATCACCTGCGGCAACTGCCGGGAGAACACCGACACGGCAAAg             <  1:468665/150‑1 (MQ=255)                                                                                                                                       tcggtcggCACCTGCGGCAGCAGGC‑GCGGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCAGCACGCGGGCCAGCATCACCTGCGGCAACTGCCGGGAGAACACCGACACGGCAAAg             <  1:679298/150‑1 (MQ=255)                                                                                                                                        cggtcggCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCAGCACGCGGGCCAGCATCACCTGCGGCAACTGCCGGGAGAACACCGACACGGCAAAgg            <  1:1648330/150‑1 (MQ=255)                                                                                                                                            cagcgccTGCTGCCGCAGGT‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCAGCACGCGGGCCAGCATCACCTGCGGCAACTGCCGGGAGAACACCGACACGGCAAAGGTCCa        >  1:54848/6‑150 (MQ=255)                                                                                                                                                aCCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCAGCACGCGGGCCAGCATCACCTGCGGCAACTGCCgg                                  <  1:2313490/120‑1 (MQ=255)                                                                                                                                                aCCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCAGCACGCGGGCCAGCATCACCTGCGGCAACTGCCgg                                  >  2:2313490/1‑120 (MQ=255)                                                                                                                                                  cTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCAGCACGCGGGCCAGCATCACCTGCGGCAACTGCCGGGAGAACACCGacac                    >  2:759209/1‑132 (MQ=255)                                                                                                                                                  cTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCAGCACGCGGGCCAGCATCACCTGCGGCAACTGCCGGGAGAACACCGacac                    <  1:759209/132‑1 (MQ=255)                                                                                                                                                  cTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCAGCACGCGGGCCAGCATCACCTGCGGCAACTGCCGGGAGAACACCGACACGGCAAAGGTCCAgccgcc  >  1:1786359/1‑150 (MQ=255)                                                                                                                                                      |                                                                                                                                                   CGTCCAGCCGGCCGCCGAGCAAGGCGGCAATGGCGAACGCGAAAGCCACCGAGAAGCCCACATAGCCCATGTAGAGCATGGGTGGGTGAACGATCAGGCCAAAGTCCTGCAACAGCGGGTTGAGGTCACGGCCATCGGTCGGCACCTGCGGCAGCAGGC‑GCTGGAACGGGTTGGAAGTAATGATCAGGAAGCTCAGGAAGCCCACGCTGATCATGCCCATTACCGCCAGCACGCGGGCCAGCATCACCTGCGGCAACTGCCGGGAGAACACCGACACGGCAAAGGTCCAGCCGCC  >  NC_002947/4914125‑4914419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑