Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,160,484 C→A 5.3% D207Y (GAC→TAC)  PP_4543 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,160,4840CA 5.3% 296.4 / 2.5 93D207Y (GAC→TAC) PP_4543hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (46/42);  new base A (2/3);  total (48/45)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

GTTGGCATAGCATAGAAAGACGAAATCCCACTGTGCCTCGTCCAGTTCATCACCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGCCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATA  >  NC_002947/5160335‑5160628
                                                                                                                                                     |                                                                                                                                                
gtTGGCATAGCATAGAAAGACGAAATCCCACTGTGCCTCGTCCAGTTCATCACCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTc                                                                                                                                                  >  1:1560525/1‑150 (MQ=255)
   ggCATAGCATAGAAAGACGAAATCCCACTGTGCCTCGTCCAGTTCATCACCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTg                                                                                                                                               <  2:1560525/150‑1 (MQ=255)
    gcatagcataGAAAGACGAAATCCCACTGTGCCTCGTCCAGTTCATCACCGCGGTCCCAGCGGCAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGCGAAAGTCCTgg                                                                                                                                              >  1:607289/1‑150 (MQ=255)
    gcatagcataGAAAGACGAAATCCCACTGTGCCTCGTCCAGTTCATCACCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTgg                                                                                                                                              <  2:2056220/150‑1 (MQ=255)
      atagcatagAAAGACGAAATCCCACTGTGCCTCGTCCAGTTCATCACCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTTCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGaa                                                                                                                                            >  2:1968956/1‑150 (MQ=255)
           atgcaaaGACTAGATCCCACTGTGCCTCGTCCAGTTCATCACCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTcgc                                                                                                                                       <  2:257773/146‑1 (MQ=255)
            tagAAAGACGAAATCCCACTGTGCCTCGTCCAGTTCATCACCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTcgcg                                                                                                                                      >  1:273432/1‑150 (MQ=255)
              gAAAGACGAAATCCCACTGTGCCTCGTCCAGTTCATCACCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGAt                                                                                                                                    <  1:606355/150‑1 (MQ=255)
               aaaGACGAAATCCCACTGTGCCTCGTCCAGTTCATCACCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATa                                                                                                                                   >  2:2161497/1‑150 (MQ=255)
                aaGACGAAATCCCACTGTGCCTCGTCCAGTTCATCACCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATAc                                                                                                                                  <  1:96076/150‑1 (MQ=255)
                aaGACGAAATCCCACTGTGCCTCGTCCAGTTCATCACCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATAc                                                                                                                                  >  2:1076614/1‑150 (MQ=255)
                 aGACGAAATCCCACTGTGCCTCGTCCAGTTCATCACCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGGCTGCGCTCCTTGCGCATCTGCTTGAGCCCACGCGAGCGGAGGCTGGCGAGAAAGTCCTGGAAGTCGCGATAcc                                                                                                                                 >  1:1343333/1‑150 (MQ=255)
                   tcGAAATCCCACTGTGCCTCGTCCAGTTCATCACCGAGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATAcccc                                                                                                                               <  1:501047/149‑1 (MQ=255)
                     gAAATCCCACTGTGCCTCGTCCAGTTCATCACCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGATCTGAGGCTGTCGAGAAAGTCCTTGAAGTCGCGATAcccaca                                                                                                                             >  2:501047/1‑147 (MQ=255)
                          cccACTGTGCCTCGTCCAGTTCATCACCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGtttt                                                                                                                        <  2:1396119/150‑1 (MQ=255)
                            cACTGTGCCTCGTCCAGTTCATCACCGCGGTACCAGCGGAATTCGATACCCTGCCCCGCCCCCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGCCGTGGGAGTCGCGATACCCCCGGGTTTGc                                                                                                                      >  1:864788/1‑150 (MQ=255)
                            cACTGTGCCTCGTCCAGTTCATCACCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATAc                                                                                                                                  <  1:2317539/138‑1 (MQ=255)
                            cACTGTGCCTCGTCCAGTTCATCACCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATAc                                                                                                                                  >  2:2317539/1‑138 (MQ=255)
                            cACTGTGCCTCGTCCAGTTCATCACCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGc                                                                                                                      <  1:202416/150‑1 (MQ=255)
                            cACTGTGCCTCGTCCAGTTCATCACCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGCACTGTAAGTCGCGATACCCCCGGTTTTGc                                                                                                                      <  1:2412040/150‑1 (MQ=255)
                               tgtgCCTCGTCCAGTTCATCACCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAg                                                                                                                   >  1:1590944/1‑150 (MQ=255)
                                    cTCGTCCAGTTCATCACCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGaaa                                                                                                              >  1:1658488/1‑150 (MQ=255)
                                         ccAGTTCATCACCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTcg                                                                                                                                        <  1:303204/119‑1 (MQ=255)
                                         ccAGTTCATCACCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTcg                                                                                                                                        >  2:303204/1‑119 (MQ=255)
                                          cAGTTCATCACCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCa                                                                                                        >  1:860119/1‑150 (MQ=255)
                                           aGTTCATCACCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTTTCACTGGCaa                                                                                                       <  2:1857636/150‑2 (MQ=255)
                                           aGTTCATCACCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAg                                                                                                       >  2:499496/1‑150 (MQ=255)
                                           aGTTCATCACCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAg                                                                                                       >  1:353368/1‑150 (MQ=255)
                                           aGTTCATCACCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAg                                                                                                       >  1:177269/1‑150 (MQ=255)
                                               catcaCCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCa                                                                                                   <  2:803552/150‑1 (MQ=255)
                                                atcaCCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGttt                                                                                                                         >  2:1647109/1‑127 (MQ=255)
                                                atcaCCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGttt                                                                                                                         <  1:1647109/127‑1 (MQ=255)
                                                 tcaCCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAgg                                                                                                 >  2:203275/1‑150 (MQ=255)
                                                  caCCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCGCCGGTTTTGCCAGTGAAACTGGCAgaaccggg                                                                                                <  2:1343333/150‑8 (MQ=255)
                                                  caCCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGc                                                                                                <  2:2055363/150‑1 (MQ=255)
                                                   aCCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCg                                                                                               >  1:1894501/1‑150 (MQ=255)
                                                   aCCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCg                                                                                               >  2:110632/1‑150 (MQ=255)
                                                   aCCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCg                                                                                               >  1:454011/1‑150 (MQ=255)
                                                   aCCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCg                                                                                               >  2:2404626/1‑150 (MQ=255)
                                                   aCCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCg                                                                                               >  2:1648532/1‑150 (MQ=255)
                                                   aCCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCCTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCt                                                                                                                                                >  1:1814063/1‑101 (MQ=255)
                                                   aCCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCCTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCt                                                                                                                                                <  2:1814063/101‑1 (MQ=255)
                                                    ccGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGt                                                                                              >  2:298378/1‑150 (MQ=255)
                                                     cgcgGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGtt                                                                                             <  2:2387980/150‑1 (MQ=255)
                                                          tACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTtccatc                                                                                        <  2:2255302/150‑1 (MQ=255)
                                                          tACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTtccatc                                                                                        >  1:2027436/1‑150 (MQ=255)
                                                             cAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCAc                                                                                     <  1:1076614/150‑1 (MQ=255)
                                                              aGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGACGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCAcc                                                                                    >  1:877188/1‑150 (MQ=255)
                                                               gCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGACGTCGGATTACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCAccc                                                                                   >  2:665989/1‑150 (MQ=255)
                                                                 ggAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCcag                                                                                 <  2:679469/150‑1 (MQ=255)
                                                                       cGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCTCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCaggcaggc                                                                           <  1:2090444/150‑1 (MQ=255)
                                                                        gATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGcc                                                                          >  1:1832439/1‑150 (MQ=255)
                                                                        gATACCATGCCCCGGGAGCTGCTCGCGCTCCTTGCACATCTGCTTGCGCTTGCGCTAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGcc                                                                          >  2:1282074/1‑150 (MQ=255)
                                                                             ccTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGCCCGCCa                                                                     >  1:449796/1‑150 (MQ=255)
                                                                             ccTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGCCCGCCa                                                                     >  2:120376/1‑150 (MQ=255)
                                                                              cTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGCCCGCCaa                                                                    >  1:2284623/1‑150 (MQ=255)
                                                                                  cccGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTgg                                                                                                          >  1:712820/1‑108 (MQ=255)
                                                                                  cccGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTgg                                                                                                          <  2:712820/108‑1 (MQ=255)
                                                                                  accgaccccTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAAAAAGTCCCGTCAGGACCGGATTTCGCGATACCCCCCGAGTAAAAAGTGCCCCTGGCAGTCAAAGAGTTCCAGCCACCCAGGCAGGCCCGCCAACTgc                                                                <  1:544234/143‑1 (MQ=255)
                                                                                    cGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGCCCGCCAACTgcgc                                                              >  1:1313543/1‑150 (MQ=255)
                                                                                       cACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGCCCGCCAACTGCGCGTc                                                           <  1:1587780/150‑1 (MQ=255)
                                                                                        aCCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGCCCGCCAACTGCGCGTcc                                                          >  2:503993/1‑150 (MQ=255)
                                                                                        aCCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGCCCGCCAACTGCGCGTcc                                                          <  1:1648532/150‑1 (MQ=255)
                                                                                                gcgcTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGtttt                                                                                                                        >  2:1199114/1‑80 (MQ=255)
                                                                                                gcgcTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGtttt                                                                                                                        <  1:1199114/80‑1 (MQ=255)
                                                                                                gcgcTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGCCCGCCAACTGCGCGTCCAGCGCCgg                                                  >  1:1016424/1‑150 (MQ=255)
                                                                                                gcgcTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTACTGGAAGTCGCGATAcccc                                                                                                                               <  2:1267066/73‑1 (MQ=255)
                                                                                                gcgcTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTACTGGAAGTCGCGATAcccc                                                                                                                               >  1:1267066/1‑73 (MQ=255)
                                                                                                  gcTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGACAGGCCCGCCAACTGCGCGTCCAGCGCCGGGt                                                <  1:312460/150‑1 (MQ=255)
                                                                                                    tCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGCCCGCCAACTGCGCGTCCAGCGCCGGGTCg                                              <  2:1832439/150‑1 (MQ=255)
                                                                                                      cTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGCCCGCCAACTGCGCGTCCAGCGCCGGGTCGGt                                            <  2:353368/150‑1 (MQ=255)
                                                                                                      cTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTCTAATTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGCCCGCCAACTGCGCGTCCAGCGCCGGGTCGGt                                            <  1:1968956/150‑1 (MQ=255)
                                                                                                       ttGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCATTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGCCCGCCAACTgc                                                                <  1:2132652/129‑1 (MQ=255)
                                                                                                       ttGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGCCCGCCAACTgc                                                                >  2:2132652/1‑129 (MQ=255)
                                                                                                         gcgcATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGCCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGaa                                         <  2:1313543/150‑1 (MQ=255)
                                                                                                            cATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGCCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAgct                                      >  1:1816992/1‑148 (MQ=255)
                                                                                                             aTCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGCCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTg                                     >  1:57322/1‑150 (MQ=255)
                                                                                                              tCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGCCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGa                                    <  2:273432/150‑1 (MQ=255)
                                                                                                               cTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGCCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGAt                                   <  1:203275/150‑1 (MQ=255)
                                                                                                                 gcttgcgcttgcGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGCCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGAtgt                                 >  1:2010190/1‑150 (MQ=255)
                                                                                                                 gcttgcgcttgcGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGCCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGAtgt                                 <  2:1016424/150‑1 (MQ=255)
                                                                                                                        cttgcgcGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGCCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTcc                          <  2:877188/150‑1 (MQ=255)
                                                                                                                             gcgAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGCCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGaaa                     >  1:1359118/1‑150 (MQ=255)
                                                                                                                             gcgAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGCCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGACGTGCGCTCCGGaaa                     <  1:2404626/150‑1 (MQ=255)
                                                                                                                                  cTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGCCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCa                <  1:1282074/150‑1 (MQ=255)
                                                                                                                                  cTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGCCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCa                <  2:1658488/150‑1 (MQ=255)
                                                                                                                                   tGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCACACCCCGCCCCCGGGTGGCCAGAGCCGGCTCGTTGAAGTTGATGTGCGACCAGGCAAGCCCAc               >  2:564656/1‑150 (MQ=255)
                                                                                                                                     aGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGCCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCAccc             <  1:503993/150‑1 (MQ=255)
                                                                                                                                       gCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGCCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCtt           <  2:3559/150‑1 (MQ=255)
                                                                                                                                             gagaAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGCCCGCCAACTGCGCGTCCAGCGCCGGGGCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAg     >  1:2323728/1‑150 (MQ=255)
                                                                                                                                             gagaAAGTACTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGCCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAg     >  1:2247190/1‑150 (MQ=255)
                                                                                                                                              agaAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGCCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGa    <  2:860119/150‑1 (MQ=255)
                                                                                                                                              agaAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGCCCGCCAACTGCAAGTCCAGCGCCAGTGCTGGGAAGTGTATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGa    >  1:1116603/1‑150 (MQ=255)
                                                                                                                                                aaaGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGCCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATa  <  1:120376/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
GTTGGCATAGCATAGAAAGACGAAATCCCACTGTGCCTCGTCCAGTTCATCACCGCGGTACCAGCGGAAGTCGATACCCTGCCCCGCCACCTGCTCGCGCTCCTTGCGCATCTGCTTGCGCTTGCGCGAGCTGAGGCTGTCGAGAAAGTCCTGGAAGTCGCGATACCCCCGGTTTTGCCAGTGAAACTGGCAGCCCAGGCGTTCCATCCACCCAGGCAGGCCCGCCAACTGCGCGTCCAGCGCCGGGTCGGTGAAGTTGATGTGCGCTCCGGAAAGCCCACCCTTGCCCAGATA  >  NC_002947/5160335‑5160628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑