Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,184,165 C→T 7.1% A85V (GCC→GTC)  PP_4564 → lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,184,1650CT 7.1% 320.4 / 11.4 114A85V (GCC→GTC) PP_4564lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (50/55);  new base T (2/6);  total (52/62)
Fisher's exact test for biased strand distribution p-value = 2.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.22e-01

Only 100 of 120 total aligned reads displayed.

GGTGCCGTGGTCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGAGGCCT  >  NC_002947/5184016‑5184314
                                                                                                                                                     |                                                                                                                                                     
ggTGCCGTGGTCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGc                                                                                                                                                       <  1:515168/150‑1 (MQ=255)
   gCCGTGGTCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCcga                                                                                                                                                    >  1:128316/1‑150 (MQ=255)
   gCCGTGGTCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCcga                                                                                                                                                    <  2:285458/150‑1 (MQ=255)
   gCCGTGGTCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGGGACATGGTCGCcga                                                                                                                                                    <  2:2040916/150‑1 (MQ=255)
   caggtagTCGCTCAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCcga                                                                                                                                                    >  1:1088698/7‑150 (MQ=255)
         gTCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTGCCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCa                                                                                                                                              <  2:2169267/150‑1 (MQ=255)
          tCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGCGACATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCAGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAg                                                                                                                                             >  1:436175/1‑150 (MQ=255)
            gCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGCGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACCTGGTCGTCGGCGACCATGt                                                                                                                                           <  2:80622/150‑1 (MQ=255)
            gCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACCTGGTCGTCGGCGACCATGt                                                                                                                                           <  1:329309/150‑1 (MQ=255)
            gCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGt                                                                                                                                           >  2:280777/1‑150 (MQ=255)
             atctgCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTc                                                                                                                                          >  2:406236/5‑150 (MQ=255)
              tGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCa                                                                                                                                              >  1:236028/1‑145 (MQ=255)
                                   cccGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCaaa                                                                                                                    <  2:49631/149‑1 (MQ=255)
                                   aCCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCaaa                                                                                                                    <  2:1952176/150‑1 (MQ=255)
                                   aCCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCaaa                                                                                                                    <  1:985906/150‑1 (MQ=255)
                                   aCCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCaaa                                                                                                                    <  2:1063256/150‑1 (MQ=255)
                                     cgcgAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGCCAGGGTACGTGACATGGTCGCCGCCGACCAGGTCACCGACCAGGCCCTGCAGCAAAGc                                                                                                                  >  2:2276144/1‑150 (MQ=255)
                                          gCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCcg                                                                                                             >  2:2394749/1‑150 (MQ=255)
                                            aaaGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGTCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCcgcg                                                                                                           >  1:980839/1‑150 (MQ=255)
                                             aaGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGa                                                                                                          >  1:2404894/1‑150 (MQ=255)
                                             aaGGGCGCTGCTGGATGATCGGTGGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGa                                                                                                          <  2:1791560/150‑1 (MQ=255)
                                               gggCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAgg                                                                                                        <  2:1250586/150‑1 (MQ=255)
                                                    ctgctgGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGt                                                                                                   <  2:888542/150‑1 (MQ=255)
                                                    ctgctgGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGt                                                                                                   <  1:550579/150‑1 (MQ=255)
                                                     tgctgGATGATCGGTTGCGCGAGCACCTGGCCTGGCATCGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTg                                                                                                  <  1:1370940/150‑1 (MQ=255)
                                                      gctgGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGa                                                                                                 <  2:2404894/150‑1 (MQ=255)
                                                       ctgGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGt                                                                                                       <  2:897960/143‑1 (MQ=255)
                                                       ctgGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGt                                                                                                       >  1:897960/1‑143 (MQ=255)
                                                       ctgGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAg                                                                                                >  1:470726/1‑150 (MQ=255)
                                                        tgGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACGGAGCGGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAgg                                                                                               <  1:648934/150‑1 (MQ=255)
                                                          gatgatCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCa                                                                                             >  1:905030/1‑150 (MQ=255)
                                                            tgatCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAAt                                                                                           >  1:1080706/1‑150 (MQ=255)
                                                             gatCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAAtt                                                                                          <  2:159318/150‑1 (MQ=255)
                                                              atCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAACTg                                                                                         <  2:2012986/150‑1 (MQ=255)
                                                               tCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTgg                                                                                        >  2:436927/1‑150 (MQ=255)
                                                               tCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGACAGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACAAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTgg                                                                                        >  1:436310/1‑150 (MQ=255)
                                                                tcatgtcgcgAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACAGGCTAAGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCGAAGGGCCGGGGAGGTGCGTGAGGCAATGGGt                                                                                       >  1:344344/7‑150 (MQ=255)
                                                                cGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGt                                                                                       <  2:1246384/150‑1 (MQ=255)
                                                                  gTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACCTGGTCGTCGGCGACCATGTc                                                                                                                                          >  1:1722250/1‑97 (MQ=38)
                                                                  gTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACCTGGTCGTCGGCGACCATGTc                                                                                                                                          <  2:1722250/97‑1 (MQ=38)
                                                                   ttGCTCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGa                                                                                    <  2:2381051/150‑1 (MQ=255)
                                                                     gcgcGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCAGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGAtt                                                                                  <  2:236805/150‑1 (MQ=255)
                                                                     cctcgAGCACCTGGCCTGGCATGGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGAtt                                                                                  <  2:508492/147‑1 (MQ=255)
                                                                        cgAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGc                                                                               <  2:470726/150‑1 (MQ=255)
                                                                         gAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGt                                                                                                   >  1:869733/1‑129 (MQ=255)
                                                                         gAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGt                                                                                                   <  2:869733/129‑1 (MQ=255)
                                                                         gAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGcc                                                                              >  1:1322373/1‑150 (MQ=255)
                                                                           gCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCCGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGa                                                                            >  2:1660538/1‑150 (MQ=255)
                                                                           gCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGGCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGa                                                                            >  2:1708375/1‑150 (MQ=255)
                                                                               ctggcctggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGCCCAGGTCAc                                                                                                                                        >  1:1223574/1‑86 (MQ=255)
                                                                               ctggcctggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCAc                                                                                                                                        <  2:1223574/86‑1 (MQ=255)
                                                                               ctggcctggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGaa                                                                        >  1:1395989/1‑150 (MQ=255)
                                                                                tggcctggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGaaa                                                                       <  1:923728/150‑1 (MQ=255)
                                                                                tggcctggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGaaa                                                                       <  2:1395989/150‑1 (MQ=255)
                                                                                    ctggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGCCGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTgg                                                                                <  2:1102028/137‑1 (MQ=255)
                                                                                    ctggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTg                                                                                         <  1:507664/128‑1 (MQ=255)
                                                                                    ctggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTg                                                                                         >  2:507664/1‑128 (MQ=255)
                                                                                    ctggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTgg                                                                                >  1:1102028/1‑137 (MQ=255)
                                                                                    ctggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCAc                                                                   <  1:2394749/150‑1 (MQ=255)
                                                                                    ctggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCAc                                                                   >  2:850242/1‑150 (MQ=255)
                                                                                     tggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCAcc                                                                  <  1:508296/150‑1 (MQ=255)
                                                                                      ggcATTGCAAGCCTCAACTGCCCGGCTACCTCGACTGGCTCGTCAGGCTGAGATAAGGGCTCGCCGACCGCCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCAccc                                                                 <  1:280777/150‑1 (MQ=255)
                                                                                           ggCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCg                                                            >  2:1059513/2‑150 (MQ=255)
                                                                                            gCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCgg                                                           <  1:742862/150‑1 (MQ=255)
                                                                                                 aCTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCAcc                                                      >  2:2134852/1‑150 (MQ=255)
                                                                                                   tCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCAcc                                                      <  2:2258917/148‑1 (MQ=255)
                                                                                                   tCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCAcc                                                      >  1:2258917/1‑148 (MQ=255)
                                                                                                   tCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGa                                                    >  2:2406658/1‑150 (MQ=255)
                                                                                                        tGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAActgc                                               >  1:287255/1‑150 (MQ=255)
                                                                                                        tGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTCACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCTGATTTAATCACCCCTTCGGCCACCGAActgc                                               >  2:2133574/1‑150 (MQ=255)
                                                                                                           ccGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAAAtgctgc                                            >  1:480079/1‑150 (MQ=255)
                                                                                                              gCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGcgcg                                         <  1:2191919/150‑1 (MQ=255)
                                                                                                                 aCCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCa                                      <  2:287255/150‑1 (MQ=255)
                                                                                                                  ccTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGGCGACCGGGTCACCGACCAGGCGCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCAt                                     >  2:1966693/1‑150 (MQ=255)
                                                                                                                  ccTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAGTCACCCCTTCGGCCAACGCACTGCGGCGCGGCAt                                     >  2:1239592/1‑150 (MQ=255)
                                                                                                                   cTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGCCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATg                                    >  2:332964/1‑150 (MQ=255)
                                                                                                                    tCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGa                                   <  1:1641348/150‑1 (MQ=255)
                                                                                                                      gACTGGCTCGACTCGCGGCGGGACATGGTAGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGTCCACCGAACTGCTGCGCGGCATGAGc                                 <  2:436175/150‑1 (MQ=255)
                                                                                                                         tGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGAc                              <  2:1088698/150‑1 (MQ=255)
                                                                                                                            cTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTAGATTGGCCGAGGAAATCACCCCTTCGGCCAACGAACTGCTGCGCGGCATGAGCGACAAc                           >  2:182599/1‑150 (MQ=255)
                                                                                                                             tCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAAcc                          >  2:412272/1‑150 (MQ=255)
                                                                                                                             tCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAAcc                          >  1:1358330/1‑150 (MQ=255)
                                                                                                                                  cGGCTACGGGACATGGTCGCCGCCGACCAGGACACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTa                     <  2:556231/150‑1 (MQ=255)
                                                                                                                                    gCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAgc                   <  1:1067847/150‑1 (MQ=255)
                                                                                                                                      tACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTACGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAgcgc                 >  1:872554/1‑150 (MQ=255)
                                                                                                                                      tACGTGACATGGTCGCCGACGACCAGGTCACCGACAACGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAgcgc                 >  1:789853/1‑150 (MQ=255)
                                                                                                                                          tGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGAt             <  1:406236/150‑1 (MQ=255)
                                                                                                                                           gACCTGGTCGTCGGCGACCATGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAg                                                                           >  1:990170/1‑87 (MQ=38)
                                                                                                                                            acacGGTCGCCATCGACCAGGTCCCTGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTACGCGGCATGAGCGACAACCAGGTAGCGCAGATGc           <  2:1322373/150‑1 (MQ=255)
                                                                                                                                              aTGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGt         <  1:1708375/150‑1 (MQ=255)
                                                                                                                                              aTGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGt         <  1:442095/150‑1 (MQ=255)
                                                                                                                                                ggTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGa       <  2:245645/150‑1 (MQ=255)
                                                                                                                                                ggTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGa       <  1:245097/150‑1 (MQ=255)
                                                                                                                                                  tCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGAgg     <  2:1080706/150‑1 (MQ=255)
                                                                                                                                                    gCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGAGGcc   <  2:436310/150‑1 (MQ=255)
                                                                                                                                                     ccGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGa                                                                                    >  2:1970580/1‑68 (MQ=255)
                                                                                                                                                     ccGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGa                                                                                    <  1:1970580/68‑1 (MQ=255)
                                                                                                                                                     ccGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGAGGCCt  >  1:2031045/1‑150 (MQ=255)
                                                                                                                                                     ccGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGAGGCCt  >  1:2030976/1‑150 (MQ=255)
                                                                                                                                                     ccGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGAGGCCt  >  1:1980660/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGTGCCGTGGTCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGAGGCCT  >  NC_002947/5184016‑5184314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑