| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,192,823 | T→A | 10.0% | intergenic (+156/‑46) | PP_4572 → / → PP_4573 | hypothetical protein/AAA family ATPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,192,823 | 0 | T | A | 10.0% | 159.8 / 10.1 | 60 | intergenic (+156/‑46) | PP_4572/PP_4573 | hypothetical protein/AAA family ATPase |
| Reads supporting (aligned to +/- strand): ref base T (28/26); new base A (4/2); total (32/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-02 | |||||||||||
GACGGGGCATTCAGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGCCCGCTGTTGGTCAAGGGCGAACC > NC_002947/5192676‑5192972 | gACGGGGCATTCAGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTAc < 2:1246939/150‑1 (MQ=255) gCACTCAGGGACTGAATACCCGGTCTGCGTTTGCAACCGCCAGGCCCCGTGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGa < 1:587570/150‑1 (MQ=255) tCAGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGACTGTTCGGGCCTCTTCGCGGGTAACCCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAagag < 1:1992218/150‑1 (MQ=255) cAGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAgg < 1:1310995/150‑1 (MQ=255) aGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGCTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGc > 2:1260733/1‑150 (MQ=255) tGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAagag < 2:1362719/142‑1 (MQ=255) tGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAagag > 1:1362719/1‑142 (MQ=255) cccGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGTTCCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCACGAAGAGGCCCGCAAAGCCCt < 2:1979872/150‑1 (MQ=255) ggTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTaaa > 1:615177/1‑150 (MQ=255) tGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACaga < 1:1257148/150‑1 (MQ=255) tGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACaga < 1:1257767/150‑1 (MQ=255) tGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACaga < 2:615177/150‑1 (MQ=255) gCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACagag < 1:1260733/150‑1 (MQ=255) gCGNTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACagag > 2:1516873/1‑150 (MQ=255) aGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGGTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAAcc > 1:1303312/1‑150 (MQ=255) cgccAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAAccccc < 2:995892/150‑1 (MQ=255) aGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGa < 1:1516873/150‑1 (MQ=255) gcgTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGaa > 1:127338/1‑103 (MQ=255) gcgTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGaa < 2:127338/103‑1 (MQ=255) cgTTATAGTCGGAAACAAAGCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAACCCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGaa < 2:218267/150‑1 (MQ=255) tCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCcg > 1:2302421/1‑150 (MQ=255) aaaCAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTAccc > 1:2011951/1‑85 (MQ=255) aaaCAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTAccc < 2:2011951/85‑1 (MQ=255) aTCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGAGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTAAGTc > 1:2453588/1‑150 (MQ=255) tCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCg > 1:1551902/1‑150 (MQ=255) aGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCca > 1:339496/1‑150 (MQ=255) tgctgcCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGANGAc < 2:1514151/150‑1 (MQ=255) gcCTATTCGGGCCCCTCCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCCCGGGTTTACCCGCGAAGAGGCCCGCAAACCCCTAAACAGAGGACAAACCCCCATGAAGTTCGCAGGCACCCGCGACTACGTCGCCACAGATGACCTGa < 1:2290753/150‑1 (MQ=39) gCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACCGAGGACAAACCCCCATGAAGTTCGAAg > 1:1355806/1‑107 (MQ=255) gCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAg < 2:1355806/107‑1 (MQ=255) gcgGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAGACAGAGGACAAACCCCCCTGAAGTTCGAAGGCACCCGCGACTACGTCGCCCCAGATGACCTGACACTCGAGGGCAAcgcag > 2:170265/1‑148 (MQ=255) gcgGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCgg > 2:445847/1‑150 (MQ=255) ggggTAAACCCGCGCCGACAACCTCATTTTGCGCGGGTTTACCCGCGAAGAGGCACGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGNGGc < 2:1509918/149‑1 (MQ=255) cgGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGc > 1:28085/1‑150 (MQ=255) gTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCAt > 1:2012136/1‑150 (MQ=255) tAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCAtt > 1:786219/1‑150 (MQ=255) tAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCACGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCAtt > 1:2098000/1‑150 (MQ=255) cccGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCAGCTGGGACCTGAAACTGGCGGTAAACGCGGCCATTAccc > 1:1307647/1‑150 (MQ=255) ccGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGaa > 2:1297434/1‑88 (MQ=255) ccGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGaa < 1:1297434/88‑1 (MQ=255) cGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTc < 2:2302421/150‑1 (MQ=255) tACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTgg < 1:2405908/121‑1 (MQ=255) tACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTgg > 2:2405908/1‑121 (MQ=255) ccTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGCCCGCt > 1:1100697/1‑150 (MQ=255) cTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGACCTTGTCGGTAAACGCGGCCATTACCCTCGAACGCCCGCTg > 1:1947388/1‑150 (MQ=255) ttattaGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGCCCGCTGt > 1:1055480/1‑150 (MQ=255) tattaGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGCCCGCTGtt > 2:1370086/1‑150 (MQ=255) attaGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGCCCGCTGTTg < 2:28085/150‑1 (MQ=255) tcttcgcgGGTAAACCCACGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGCCCGc < 2:1889098/139‑1 (MQ=255) tcttcgcgGGTAAACCCACGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGCCCGc > 1:1889098/5‑143 (MQ=255) aGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGCCCGCTGTTGGTc < 2:339496/150‑1 (MQ=255) ttcgcgGGTAAACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTc < 2:630048/84‑1 (MQ=38) ttcgcgGGTAAACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTc > 1:630048/3‑86 (MQ=38) ttcgcgGGTAAACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGCCCGCTGTTGGTCa > 2:2347804/3‑150 (MQ=255) ttcgcgGGTAAACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGCCCGCTGTTGGTCa > 1:238054/3‑150 (MQ=255) gcgGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGCCCGCTGTTGGTCAAgg > 1:1700885/1‑150 (MQ=255) gcgGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGCCCGCTGTTGGTCAAgg < 2:151284/150‑1 (MQ=255) gcgGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCAGGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGCCCGCTGTTGGTCAAgg < 2:1055480/150‑1 (MQ=255) gTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGCCCGCTGTTGGTCAAGGGCGa > 2:1694706/1‑150 (MQ=255) gTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGCCCGCTGTTGGTCAAGGGCGa > 2:962375/1‑150 (MQ=255) tACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGCCCGCTGTTGGTCAAGGGCGAAcc > 2:2182390/1‑150 (MQ=255) | GACGGGGCATTCAGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGCCCGCTGTTGGTCAAGGGCGAACC > NC_002947/5192676‑5192972 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |