Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,257,737 A→C 5.0% V38G (GTG→GGG)  PP_4634 ← alpha/beta family hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,257,7370AC 5.0% 323.5 / 6.2 112V38G (GTG→GGG) PP_4634alpha/beta family hydrolase
Reads supporting (aligned to +/- strand):  ref base A (53/51);  new base C (2/4);  total (57/55)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 115 total aligned reads displayed.

AGGAGGCCTGGAAACCACGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAA  >  NC_002947/5257588‑5257886
                                                                                                                                                     |                                                                                                                                                     
aggaggCCTGGAAACCACGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCa                                                                                                                                                       >  1:351689/1‑150 (MQ=255)
      ccTGGAAACCACGCAGCGAAGCCGGCCAGGTTTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCg                                                                                                                                                 <  1:1568772/150‑1 (MQ=255)
       cTGGAAACCACGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCgg                                                                                                                                                <  1:1149922/150‑1 (MQ=255)
       cTGGAAACCACGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCgg                                                                                                                                                >  2:2199026/1‑150 (MQ=255)
       cTGGAAACCACGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCgg                                                                                                                                                <  2:1815869/150‑1 (MQ=255)
        tGGAAACCACGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCCCCAGCgcc                                                                                                                                               >  2:192567/1‑148 (MQ=255)
        tGGAAACCACGCAGCGAAGCCGGCCGGGATTCGCCGATGAACAGGATGTCGAGGGTCAGCACATCGCAGCCGGGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCggg                                                                                                                                               >  1:2254068/1‑150 (MQ=255)
         ggAAACCAAGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGGAGTCGTAGGTCAGAACAGCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCGGCgggg                                                                                                                                              >  2:1010073/1‑149 (MQ=255)
             aCCACGCAGCGAAGCCGGCCGGGATTAGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACAGAAGTGGCAGCGTTTATGATCTCCAGCGGGCGCTg                                                                                                                                          >  2:1203006/1‑150 (MQ=255)
              ccACGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTgg                                                                                                                                         <  2:1133296/150‑1 (MQ=255)
               cACGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTGGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTggg                                                                                                                                        <  2:2224565/150‑1 (MQ=255)
               cACGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTggg                                                                                                                                        <  2:915975/150‑1 (MQ=255)
               cACGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGCAAAGCGGTGGCGGCGTTGATGATCGCCAGCGGGCGCTggg                                                                                                                                        >  2:1840419/1‑150 (MQ=255)
                  gCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGAt                                                                                                                                     <  1:607931/150‑1 (MQ=255)
                     gCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAg                                                                                                                                  <  2:1919182/150‑1 (MQ=255)
                     gCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCCTTTATGATCCCCAGCGCCCGCTGGTGATCat                                                                                                                                  <  1:2277565/150‑2 (MQ=255)
                      cGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCACAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAgg                                                                                                                                 <  1:1864876/150‑1 (MQ=255)
                      cGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTGCCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAgg                                                                                                                                 <  2:529024/150‑1 (MQ=255)
                      cGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAgg                                                                                                                                 <  1:1361141/150‑1 (MQ=255)
                       gAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCCCCAGCGCCCGCTGGTGATCatca                                                                                                                                <  1:192567/150‑4 (MQ=255)
                         aGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATAGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTg                                                                                                                              <  2:2408654/150‑1 (MQ=255)
                          gccggccgGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCTGGTTgg                                                                                                                             >  2:1956308/1‑150 (MQ=255)
                          gccggccgGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTgg                                                                                                                             <  1:2331954/150‑1 (MQ=255)
                            cggccggGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGc                                                                                                                           <  1:569772/150‑1 (MQ=255)
                                 gggATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGgc                                                                                                                      <  2:1194232/150‑1 (MQ=255)
                                 gggATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGgc                                                                                                                      <  2:698303/150‑1 (MQ=255)
                                   gATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGgcgc                                                                                                                    >  2:502830/1‑150 (MQ=255)
                                     ttCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCACGTTGGTCGTGTCGCca                                                                                                                  <  2:746380/150‑1 (MQ=255)
                                      tCGCCGATGCCACGGTAGTCCTAGGTCAGCACATCGCAGCCGGGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCcag                                                                                                                 >  2:2231880/1‑150 (MQ=255)
                                        gCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCcagca                                                                                                               <  2:2254068/150‑1 (MQ=255)
                                           gATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGaa                                                                                                            <  1:2113799/150‑1 (MQ=255)
                                           gATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGaa                                                                                                            >  1:579149/1‑150 (MQ=255)
                                                      aGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTTACCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGc                                                                                                 <  1:1840419/150‑1 (MQ=255)
                                                          gTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTa                                                                                             <  2:1967749/150‑1 (MQ=255)
                                                          gTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTa                                                                                             >  1:1289556/1‑150 (MQ=255)
                                                            aGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATc                                                                                           >  1:2245971/1‑150 (MQ=255)
                                                             ggTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTg                                                                                                                                          <  1:2233540/102‑1 (MQ=255)
                                                             ggTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTg                                                                                                                                          >  2:2233540/1‑102 (MQ=255)
                                                                cAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTc                                                                                       <  1:945375/150‑1 (MQ=255)
                                                                 aGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCg                                                                                      >  1:1081312/1‑150 (MQ=255)
                                                                  gCACATCGCAGCCGTGGGCGAACGGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCgg                                                                                     >  2:1196203/1‑150 (MQ=255)
                                                                       tCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCcagca                                                                                                               <  2:383837/119‑1 (MQ=255)
                                                                       tCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCcagca                                                                                                               >  1:383837/1‑119 (MQ=255)
                                                                       tCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCcgcg                                                                                >  1:1575685/1‑150 (MQ=255)
                                                                          cAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCgggg                                                                             >  2:2086421/1‑150 (MQ=255)
                                                                             ccGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTg                                                                                                                                          >  2:1692121/1‑86 (MQ=255)
                                                                             ccGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTg                                                                                                                                          <  1:1692121/86‑1 (MQ=255)
                                                                                  ggCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGaaa                                                                     <  2:1897271/150‑1 (MQ=255)
                                                                                  ggCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGaaa                                                                     >  1:984893/1‑150 (MQ=255)
                                                                                   gCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCCGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAAt                                                                    <  1:1203006/150‑1 (MQ=255)
                                                                                    cGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATg                                                                   <  1:1319835/150‑1 (MQ=255)
                                                                                         aGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAGTGGGGTa                                                              >  2:1079734/1‑150 (MQ=255)
                                                                                           gTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGc                                                            >  2:698085/1‑150 (MQ=255)
                                                                                           gTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGc                                                            >  2:1130993/1‑150 (MQ=255)
                                                                                            tAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGCAATGGGGTAGCg                                                           >  1:451231/1‑150 (MQ=255)
                                                                                              gTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCGTCCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCggg                                                         >  1:1843611/1‑150 (MQ=255)
                                                                                              gTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCggg                                                         <  1:1196203/150‑1 (MQ=255)
                                                                                               tCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTAt                                                                                            <  1:1699144/114‑1 (MQ=255)
                                                                                               tCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTAt                                                                                            >  2:1699144/1‑114 (MQ=255)
                                                                                               tCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGt                                                        >  1:2440223/1‑150 (MQ=255)
                                                                                               tCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAAGGGGGGAGCGGGt                                                        >  2:1322968/1‑150 (MQ=255)
                                                                                                 ggCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTg                                                      >  2:752007/1‑150 (MQ=255)
                                                                                                 ggCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTg                                                      >  2:1247340/1‑150 (MQ=255)
                                                                                                 ggCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTg                                                      <  1:1130993/150‑1 (MQ=255)
                                                                                                 ggCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCAACACAGGTCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTg                                                      <  1:2231880/150‑1 (MQ=255)
                                                                                                  gCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTgg                                                     >  1:2208690/1‑150 (MQ=255)
                                                                                                  gCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTgg                                                     >  2:2066640/1‑150 (MQ=255)
                                                                                                   cAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGa                                                    <  1:1171313/150‑1 (MQ=255)
                                                                                                    aaaGCGGGCGTAATAGCGGAAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGAt                                                   >  2:141524/1‑150 (MQ=255)
                                                                                                      aGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGa                                                 <  1:502830/150‑1 (MQ=255)
                                                                                                       gCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAg                                                >  2:2213407/1‑150 (MQ=255)
                                                                                                        cGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAgg                                               <  2:2245971/150‑1 (MQ=255)
                                                                                                            cGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTAc                                           <  2:2208690/150‑1 (MQ=255)
                                                                                                                  aGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATgg                                     <  2:351689/150‑1 (MQ=255)
                                                                                                                           gTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTcc                            <  1:1928566/150‑1 (MQ=255)
                                                                                                                              ccGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGa                                                    <  1:640445/123‑1 (MQ=255)
                                                                                                                              ccGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGa                                                    >  2:640445/1‑123 (MQ=255)
                                                                                                                               cGAAGTGGCAGCGTTTATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCtgtt                        >  2:759246/1‑150 (MQ=255)
                                                                                                                                  aGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCtgttgtt                     <  1:2213407/150‑1 (MQ=255)
                                                                                                                                   gTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCtgttgttg                    <  2:1575685/150‑1 (MQ=255)
                                                                                                                                    tGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCtgttgttgt                   >  2:2380428/1‑150 (MQ=255)
                                                                                                                                      gCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTa                                            >  1:1502736/1‑123 (MQ=255)
                                                                                                                                      gCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTa                                            <  2:1502736/123‑1 (MQ=255)
                                                                                                                                      gCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTgc                 <  1:2066640/150‑1 (MQ=255)
                                                                                                                                       cAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTgcg                >  1:2184721/1‑150 (MQ=255)
                                                                                                                                          ccaacctgatCCCCAGCGCCCGCTGGTGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCa             >  2:2023253/7‑150 (MQ=255)
                                                                                                                                           gTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCaa            >  2:2036836/1‑150 (MQ=255)
                                                                                                                                            ttGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCaaa           >  2:2426213/1‑150 (MQ=255)
                                                                                                                                            ttGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCaaa           >  1:445385/1‑150 (MQ=255)
                                                                                                                                              gatgatCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAAc         >  1:2087083/1‑150 (MQ=255)
                                                                                                                                                tgatCCCCAGCGCCCGCTGGTGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTa       <  1:2023253/150‑1 (MQ=255)
                                                                                                                                                 gatCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTg                                                      <  1:1785212/102‑1 (MQ=255)
                                                                                                                                                 gatCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTg                                                      >  2:1785212/1‑102 (MQ=255)
                                                                                                                                                 gatCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAg      <  1:2314176/150‑1 (MQ=255)
                                                                                                                                                  atCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGc     <  1:752007/150‑1 (MQ=255)
                                                                                                                                                   tCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGt                              <  2:396217/124‑1 (MQ=255)
                                                                                                                                                    cACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGa                                                 <  1:1197944/104‑1 (MQ=255)
                                                                                                                                                    cACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGa                                                 >  2:1197944/1‑104 (MQ=255)
                                                                                                                                                     aCCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCaaa  <  1:1247340/150‑1 (MQ=255)
                                                                                                                                                     aCCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCaaa  <  1:2099704/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGGAGGCCTGGAAACCACGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAA  >  NC_002947/5257588‑5257886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑