Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,338,098 C→G 10.7% intergenic (+57/‑227) PP_4696 → / → PP_mr53 nitrogen regulation protein NR(I)/CrcZ ncRNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,338,0980CG10.7% 250.6 / 19.7 84intergenic (+57/‑227)PP_4696/PP_mr53nitrogen regulation protein NR(I)/CrcZ ncRNA
Reads supporting (aligned to +/- strand):  ref base C (36/39);  new base G (5/4);  total (41/43)
Fisher's exact test for biased strand distribution p-value = 7.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.51e-01

AAGCTCGGCGTAAGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACATTTTGAACCTGC  >  NC_002947/5337955‑5338247
                                                                                                                                               |                                                                                                                                                     
aaGCTCGGCGTAAGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCcgcgcg                                                                                                                                                 >  2:448091/1‑150 (MQ=255)
    tCGGCGTAAGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGgcgc                                                                                                                                             >  1:1494202/1‑150 (MQ=255)
    tCGGCGTAAGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGgcgc                                                                                                                                             >  2:2249931/1‑150 (MQ=255)
             gCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCgcggc                                                                                                                                               <  1:1636645/139‑1 (MQ=255)
             gCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCgcggc                                                                                                                                               >  2:1636645/1‑139 (MQ=255)
             gCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTca                                                                                                                                    <  2:777852/150‑1 (MQ=255)
               cGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTcaca                                                                                                                                  <  2:1792476/150‑1 (MQ=255)
                    gTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCCCGGCACAAGAGTAACGCGACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGgcgc                                                                                                                             >  1:1004502/1‑150 (MQ=255)
                         tgtgGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTGACAGGCGCTCAAc                                                                                                                        >  1:1939646/1‑150 (MQ=255)
                          gtgGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCGAATCGCGGCCTATGCGGCGTCGGTGAGACCGAGCGCTGCCAGAGCGGCGCTCTGTCTCACAGGCGCTGAACg                                                                                                                       >  1:644181/1‑150 (MQ=255)
                          gtgGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACg                                                                                                                       >  1:2033781/1‑150 (MQ=255)
                           tgGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACgt                                                                                                                      <  1:171194/150‑1 (MQ=255)
                           tgGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACgt                                                                                                                      <  2:1615170/150‑1 (MQ=255)
                             ggAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACgtgt                                                                                                                    >  1:2446779/1‑150 (MQ=255)
                             ggAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACgtgt                                                                                                                    <  2:1108639/150‑1 (MQ=255)
                              gAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTc                                                                                                                   <  1:2249931/150‑1 (MQ=255)
                                  gccgccAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCacga                                                                                                               <  2:950221/150‑1 (MQ=255)
                                   ccgccAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCacgac                                                                                                              <  2:2446779/150‑1 (MQ=255)
                                       cAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAac                                                                                                          <  1:2032723/150‑1 (MQ=255)
                                       cAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAac                                                                                                          >  1:2089069/1‑150 (MQ=255)
                                            ccTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACAcccc                                                                                                     <  2:2109770/150‑1 (MQ=255)
                                            ccTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACAcccc                                                                                                     <  1:206779/150‑1 (MQ=255)
                                               gggCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAgg                                                                                                  <  1:770575/150‑1 (MQ=255)
                                                ggCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGt                                                                                                 >  2:1599045/1‑150 (MQ=255)
                                                   acccccCGGCGCAAGCGCAACGCTAGCAGCGAATCACCCACCTACGCACCGCACGCCGCAGCCTAGGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAac                                                                                              <  2:858798/148‑1 (MQ=39)
                                                    tCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAaca                                                                                             <  1:1823438/150‑1 (MQ=255)
                                                     cccACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAacac                                                                                            >  2:754586/1‑150 (MQ=255)
                                                          ggCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAAcacctcac                                                                                       <  1:2186347/150‑1 (MQ=255)
                                                             gcAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACacctcacctc                                                                                    <  2:1616165/150‑1 (MQ=255)
                                                               aaGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAGCGTGTCACGACGAACACCCCa                                                                                                    <  2:940114/132‑1 (MQ=255)
                                                               aaGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAGCGTGTCACGACGAACACCCCa                                                                                                    >  1:940114/1‑132 (MQ=255)
                                                               aaGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTccc                                                                                  <  1:2290736/150‑1 (MQ=255)
                                                               aaGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTccc                                                                                  <  1:80739/150‑1 (MQ=255)
                                                                     aaCGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTaaaa                                                                            >  1:1290613/1‑150 (MQ=255)
                                                                      aCGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTaaaaa                                                                           >  1:1894671/1‑150 (MQ=255)
                                                                          tACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTg                                                                       >  2:1020662/1‑150 (MQ=255)
                                                                             cAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGgcgc                                                                                                                             >  2:1688262/1‑93 (MQ=255)
                                                                             cAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGgcgc                                                                                                                             <  1:1688262/93‑1 (MQ=255)
                                                                             cAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCGGGTAACACCTCACCTCCCGTAAAAATCt                                                                        <  1:1412756/146‑1 (MQ=255)
                                                                             cAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCGGGTAACACCTCACCTCCCGTAAAAATCt                                                                        >  2:1412756/1‑146 (MQ=255)
                                                                               gCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTAcc                                                                  >  2:491456/1‑150 (MQ=255)
                                                                                   aTAACCCCCCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAg                                                              <  2:455110/150‑1 (MQ=255)
                                                                                       cccACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACgtgt                                                                                                                    >  1:337990/1‑92 (MQ=255)
                                                                                       cccACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACgtgt                                                                                                                    <  2:337990/92‑1 (MQ=255)
                                                                                          aCCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATc                                                                         >  2:2225081/1‑132 (MQ=255)
                                                                                          aCCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATc                                                                         <  1:2225081/132‑1 (MQ=255)
                                                                                          aCCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTAc                                                                   <  2:418244/138‑1 (MQ=255)
                                                                                          aCCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTAc                                                                   >  1:418244/1‑138 (MQ=255)
                                                                                          aCCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTccg                                                       >  1:2452575/1‑150 (MQ=255)
                                                                                            cTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTccgcc                                                     <  2:1004502/150‑1 (MQ=255)
                                                                                            cTACGCACCGCACGCCGCAGCCTACGGGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTccgcc                                                     >  1:2157937/1‑150 (MQ=255)
                                                                                                  accgcacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACa                                               <  1:1599045/150‑1 (MQ=255)
                                                                                                  accgcacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACa                                               <  1:2004026/150‑1 (MQ=255)
                                                                                                   ccgcacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAg                                              >  2:2083519/1‑150 (MQ=255)
                                                                                                     gcacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTc                                            >  2:410771/1‑150 (MQ=255)
                                                                                                     gcacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTc                                            >  2:167706/1‑150 (MQ=255)
                                                                                                      cacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTcc                                           <  2:1894671/150‑1 (MQ=255)
                                                                                                      cacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTcc                                           >  1:2046812/1‑150 (MQ=255)
                                                                                                       acGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCATAACAGGccc                                          >  2:1239154/1‑150 (MQ=255)
                                                                                                         gccgcAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCg                                        >  2:1883120/1‑150 (MQ=255)
                                                                                                          ccgcAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAGCGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGa                                       <  2:1893052/150‑1 (MQ=255)
                                                                                                          ccgcAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGa                                       <  1:1883120/150‑1 (MQ=255)
                                                                                                           cgcAGCTGAGGCGGCTTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAg                                      <  2:2089069/150‑1 (MQ=255)
                                                                                                                ccTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTa                                 <  2:2118794/150‑1 (MQ=255)
                                                                                                                  tATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACg                               >  1:842485/1‑150 (MQ=255)
                                                                                                                    tGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGACGCTGAACGTGTCACGACGAACACCCCAGGTaa                                                                                               >  2:1069626/1‑84 (MQ=255)
                                                                                                                    tGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGACGCTGAACGTGTCACGACGAACACCCCAGGTaa                                                                                               <  1:1069626/84‑1 (MQ=255)
                                                                                                                       ggcgTCGGTGAGCCCGAGCGCCGCCCGCGCGGCGCTCGGCCGCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAAc                          <  2:2360491/150‑1 (MQ=255)
                                                                                                                        gcgcctgTGAGGCCGAGCGCCGCGCGGGCGGCGCTCGGTCTCGCCGACGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACg                         >  1:1869001/7‑150 (MQ=37)
                                                                                                                           tCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGt                                                                      >  2:1166603/1‑102 (MQ=255)
                                                                                                                           tCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGt                                                                      <  1:1166603/102‑1 (MQ=255)
                                                                                                                           cctgTGAGGCCGAGCGCCGCGCGGGCGGCGCTCGGTCTCACCGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCt                                                                        <  2:2221774/97‑1 (MQ=21)
                                                                                                                           cctgTGAGGCCGAGCGCCGCGCGGGCGGCGCTCGGTCTCACCGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCt                                                                        >  1:2221774/4‑100 (MQ=21)
                                                                                                                            ctgTGAGGCCGAGCGCCGCGCGGGCGGCGCTCGGTCTCACCGACGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTAc                                                                   >  2:218859/3‑104 (MQ=21)
                                                                                                                            ctgTGAGGCCGAGCGCCGCGCGGGCGGCGCTCGGTCTCACCGACGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTAc                                                                   <  1:218859/102‑1 (MQ=21)
                                                                                                                             ggTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACacctcacctc                                                                                    >  2:1772939/1‑86 (MQ=255)
                                                                                                                             ggTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACacctcacctc                                                                                    <  1:1772939/86‑1 (MQ=255)
                                                                                                                              gTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCaa                                                               >  1:1619567/1‑106 (MQ=255)
                                                                                                                              gTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCaa                                                               <  2:1619567/106‑1 (MQ=255)
                                                                                                                                     cGAGCGCCGCGCGGGCGGCGCTCGGTCTCACCGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTa                                                                    >  2:2288425/1‑94 (MQ=37)
                                                                                                                                     cGAGCGCCGCGCGGGCGGCGCTCGGTCTCACCGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTa                                                                    <  1:2288425/94‑1 (MQ=37)
                                                                                                                                     cGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACAtt            >  1:1122372/1‑150 (MQ=255)
                                                                                                                                      gAGCGCCGCGCGGGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTccgccg                                                    >  2:729844/1‑109 (MQ=255)
                                                                                                                                      gAGCGCCGCGCGGGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTccgccg                                                    <  1:729844/109‑1 (MQ=255)
                                                                                                                                             gcccgcGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACATTTTGAACCt    <  1:2083519/150‑1 (MQ=255)
                                                                                                                                               ccgcGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACATTTTGAACCTGc  >  1:1683847/1‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
AAGCTCGGCGTAAGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACATTTTGAACCTGC  >  NC_002947/5337955‑5338247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑