Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 495,969 A→T 8.5% L23Q (CTG→CAG)  PP_0408 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947495,9690AT 8.5% 306.3 / 17.7 108L23Q (CTG→CAG) PP_0408hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (44/53);  new base T (5/4);  total (50/58)
Fisher's exact test for biased strand distribution p-value = 7.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.53e-01

Only 100 of 110 total aligned reads displayed.

CGGCCTGCAGGGTCTGCTGCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAA  >  NC_002947/495823‑496118
                                                                                                                                                  |                                                                                                                                                     
cGGCCTGCAGGGTCTGCTGCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAggg                                                                                                                                                    <  2:539216/150‑1 (MQ=255)
   ccTGCAGGGTCTGCTGCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGgcgc                                                                                                                                                 <  2:2267758/150‑1 (MQ=255)
    cTGCAGGGTCTGCTGCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGcc                                                                                                                                                >  2:1217041/1‑150 (MQ=255)
      gCAGGGCCTCCTGCGCAGCCTCGGCCCGCTGCCGTTCCGGCGTTGGTGTTTCGGCCGGTCTGCTTTCCTGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGCCCTGGCCGCCGa                                                                                                                                              <  2:1103722/150‑1 (MQ=255)
        aGGGTCTGCTGCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGt                                                                                                                                            <  1:916074/150‑1 (MQ=255)
         gggTCTGCTGCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTg                                                                                                                                           <  1:1630757/150‑1 (MQ=255)
         gggTCTGCTGCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGCCCTGGCCGCCGatgc                                                                                                                                           <  2:1351360/150‑4 (MQ=255)
            tCTGCTGCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGaa                                                                                                                                        <  2:786401/150‑1 (MQ=255)
            tCTGCTGCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGaa                                                                                                                                        <  1:1217041/150‑1 (MQ=255)
            tCTGCTGCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGaa                                                                                                                                        <  1:1299153/150‑1 (MQ=255)
             ctgctgCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCTGCATCGGCGGCCAGGGCGCCGAGTGGAAg                                                                                                                                       <  2:2104362/150‑1 (MQ=255)
                ctgctCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGAc                                                                                                                                    <  1:358010/150‑1 (MQ=255)
                 tgctCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGAcc                                                                                                                                   >  1:185764/1‑150 (MQ=255)
                    tCAGCCTTGGGCAGCAGCCGTTCCAGCGCCCGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGTGCTTCGGCAGCCGGTGCCATGGCATTTGCCGGTGGTTTTTCGGCATCGGCGGCCCGGGTGCCTAGTGCAAGGACCGAg                                                                                                                                <  2:490132/150‑1 (MQ=255)
                    tCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAg                                                                                                                                >  2:108263/1‑150 (MQ=255)
                    tCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAg                                                                                                                                >  2:1428222/1‑150 (MQ=255)
                    tCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAg                                                                                                                                <  2:1780922/150‑1 (MQ=255)
                      aGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGc                                                                                                                              >  2:2438652/1‑150 (MQ=255)
                        ccTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCTGAGTGGAAGGACCGAGGCCa                                                                                                                            <  1:2452618/150‑1 (MQ=255)
                        ccTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCa                                                                                                                            >  1:1574336/1‑150 (MQ=255)
                        ccTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCa                                                                                                                            >  2:687140/1‑150 (MQ=255)
                        ccTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCa                                                                                                                            >  1:72042/1‑150 (MQ=255)
                        ccTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCa                                                                                                                            <  2:185764/150‑1 (MQ=255)
                          ttGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGCGTGGCAGGACCGAGTCCAgg                                                                                                                          >  2:897093/1‑150 (MQ=255)
                               cagcagCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGgcagca                                                                                                                     <  1:1426960/150‑1 (MQ=255)
                               cagcagCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGgcagca                                                                                                                     <  2:1859766/150‑1 (MQ=255)
                                   agcCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGCGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACAtt                                                                                                                 <  2:215263/150‑1 (MQ=255)
                                     ccGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGc                                                                                                               >  1:1978528/1‑150 (MQ=255)
                                       gTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCGTCTGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCa                                                                                                             >  1:293066/1‑150 (MQ=255)
                                       gTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGNGGCCAGNGCGCCGAGTGGANGGACCGAGGCCAGGCAGCACATTGNCa                                                                                                             <  1:2438652/150‑1 (MQ=255)
                                        ttCCAGCGCCAGTGCATCCTCCTGGATGCGTTCCAGCAAGGACGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCCGAATTGGCGTCCAGGGCGCCGAGTTGGAGGACCGAGGCAACGCAGCACAttctcag                                                                                                            >  2:563694/1‑145 (MQ=255)
                                           cAGCGCCAGTGCATCCTCCTGGCTGCGTGCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGcgt                                                                                                         <  2:174431/150‑1 (MQ=255)
                                               gcCAGTGCATCCTACTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTg                                                                                                     >  1:1662658/1‑150 (MQ=255)
                                                  aGTTCATCCTCCTGGCTGCGTTACAATCAGGGCGGTTGTGGGGCCTGGGCGGCCGCGCCCATGGCATTTGAAGGTTGTTTTCCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGa                                                                                                  <  2:23108/150‑1 (MQ=255)
                                                    tGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATa                                                                                                >  2:56870/1‑150 (MQ=255)
                                                    tGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATa                                                                                                <  2:1411992/150‑1 (MQ=255)
                                                      cATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATaaa                                                                                              <  2:1931720/150‑1 (MQ=255)
                                                        tcctccTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGgcag                                                                                                                       >  2:1154283/1‑123 (MQ=255)
                                                        tcctccTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGgcag                                                                                                                       <  1:1154283/123‑1 (MQ=255)
                                                          ctcctGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCGCGGCAGCACATTGCCAGCGTCGTGCGATAAAgtgt                                                                                          <  1:2018179/150‑1 (MQ=255)
                                                          ctcctGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCATCACATTGCCAGCGTCGTGCGATAAAgtgt                                                                                          <  1:687140/150‑1 (MQ=255)
                                                             ctGGCTGCGTTCTAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCAGCGGCCACGGCGCCGAGTGGCAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGa                                                                                       <  2:1326022/150‑1 (MQ=255)
                                                             ctGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGa                                                                                       <  2:1332727/150‑1 (MQ=255)
                                                                gCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGAGCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACAt                                                                                    >  1:2108519/1‑150 (MQ=255)
                                                                 cTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATg                                                                                   >  2:870156/1‑150 (MQ=255)
                                                                    cGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGt                                                                                <  1:976528/150‑1 (MQ=255)
                                                                      ttCCAGCAAGGGTGGGCGTGGGGCCTCGGCGGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTg                                                                                                     >  1:1640882/1‑127 (MQ=255)
                                                                      ttCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTg                                                                                                     <  2:1640882/127‑1 (MQ=255)
                                                                      ttCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCa                                                                              >  2:2405697/1‑150 (MQ=255)
                                                                      ttCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCa                                                                              >  1:2298923/1‑150 (MQ=255)
                                                                      ttCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCa                                                                              >  2:1555744/1‑150 (MQ=255)
                                                                      ttCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCa                                                                              >  1:30769/1‑150 (MQ=255)
                                                                        ccAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAgg                                                                                                                          <  1:108289/104‑1 (MQ=255)
                                                                        ccAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAgg                                                                                                                          >  2:108289/1‑104 (MQ=255)
                                                                             aaGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCaat                                                                       <  2:525062/150‑2 (MQ=255)
                                                                             aaGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAg                                                                       <  2:72042/150‑1 (MQ=255)
                                                                              aGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAgg                                                                      <  1:869018/150‑1 (MQ=255)
                                                                                 gTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCa                                                                   <  1:870156/150‑1 (MQ=255)
                                                                                    ggCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGaa                                                                <  2:1662658/150‑1 (MQ=255)
                                                                                    ggCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGaa                                                                <  2:30769/150‑1 (MQ=255)
                                                                                         ggggCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGaaa                                                           >  2:932239/1‑150 (MQ=255)
                                                                                             ccTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGaaa                                                           <  2:2263708/146‑1 (MQ=255)
                                                                                             ccTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGaaa                                                           >  1:2263708/1‑146 (MQ=255)
                                                                                             ccTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTGGTTCGGCATCGGGGGCCAGGGCGCGGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAAccag                                                       >  1:947608/1‑148 (MQ=255)
                                                                                              cTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGc                                                      <  2:2298923/150‑1 (MQ=255)
                                                                                                  gCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAgtgt                                                                                          <  2:1262221/110‑1 (MQ=255)
                                                                                                  gCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAgtgt                                                                                          >  1:1262221/1‑110 (MQ=255)
                                                                                                    aGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCAGAGGAAGGGGTGCCGAGTGGAAGGACCGAGTCCGTTCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTa                                                >  1:1615958/1‑150 (MQ=255)
                                                                                                     gCCGGTGCCATGGCATTTGCAGGTGGTTTTTTTTTTTTTTCGTCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTaa                                               >  2:1938337/1‑150 (MQ=255)
                                                                                                              aTGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAg                                                                                                                                <  1:2265022/60‑1 (MQ=255)
                                                                                                              aTGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAg                                                                                                                                >  2:2265022/1‑60 (MQ=255)
                                                                                                                ggCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTTTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGcc                                    >  1:1844874/1‑150 (MQ=255)
                                                                                                                ggCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGcc                                    <  2:1830230/150‑1 (MQ=255)
                                                                                                                ggCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGca                                    >  1:190363/1‑149 (MQ=255)
                                                                                                                     ttGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGa                               <  2:1574336/150‑1 (MQ=255)
                                                                                                                         aGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGc                           >  1:794670/1‑150 (MQ=255)
                                                                                                                           gtggtTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCa                         >  1:690801/1‑150 (MQ=255)
                                                                                                                           gtggtTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCa                         >  2:2162408/1‑150 (MQ=255)
                                                                                                                           gtggtTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAAACGGCACCCTAATAGTAGTTGCCCGTGTCGGCCa                         >  2:1098060/1‑150 (MQ=255)
                                                                                                                             ggtTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCAtg                       >  2:1058849/1‑150 (MQ=255)
                                                                                                                              gtTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGATTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCAtgc                      <  2:919402/150‑1 (MQ=255)
                                                                                                                               tttttCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCAtgct                     <  1:1095988/150‑1 (MQ=255)
                                                                                                                                 tttCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCAtgctgc                   <  1:1098060/150‑1 (MQ=255)
                                                                                                                                 tttCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCAtgctgc                   <  1:1098081/150‑1 (MQ=255)
                                                                                                                                   tgggCATCTGCGGCCAGGGCGCCGAGTTGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCAt                 <  2:947608/148‑1 (MQ=255)
                                                                                                                                   tCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCAt                 <  1:1754430/150‑1 (MQ=255)
                                                                                                                                    tccactCGGCGCCCTGGCCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATg                >  1:1530037/6‑150 (MQ=255)
                                                                                                                                    cGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCAGCATg                >  1:30554/1‑150 (MQ=255)
                                                                                                                                      cactCGGCGCCCTGGCCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCCTg                >  2:1087429/4‑148 (MQ=255)
                                                                                                                                      cactCGGCGCCCTGGCCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATg                <  1:1087429/145‑1 (MQ=255)
                                                                                                                                       actCGGCGCCCTGGCCGCCGAGTGGAAGGACCGAGGCCAGGCTGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCGGGATGTGAGTGGCCCGTGACGGCCATGCTGCATGGca             >  2:1592251/3‑149 (MQ=255)
                                                                                                                                       actCGGCGCCCTGGCCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGcc             <  2:1530037/148‑1 (MQ=255)
                                                                                                                                       actCGGCGCCCTGGCCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGcc             >  1:113995/3‑150 (MQ=255)
                                                                                                                                       actCGGCGCCCTGGCCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGCCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATCATGCATGGcc             >  1:2243301/3‑150 (MQ=255)
                                                                                                                                            gcggcCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCAGCATGGCCGTcac        <  1:1428222/150‑3 (MQ=255)
                                                                                                                                             cggcCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCAGCATGGCCGTcacg       <  2:30554/150‑4 (MQ=255)
                                                                                                                                                ccAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGcc    <  2:293066/150‑1 (MQ=255)
                                                                                                                                                ccAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGcc    <  1:108263/150‑1 (MQ=255)
                                                                                                                                                  aGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCaa  <  2:690801/150‑1 (MQ=255)
                                                                                                                                                  aGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCaa  <  2:794670/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
CGGCCTGCAGGGTCTGCTGCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTTGCCAA  >  NC_002947/495823‑496118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑