BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,750,455	A→G	5.1%	H388H (CAT→CAC)	thiI ←	tRNA sulfurtransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,750,455	0	A	G	5.1%	291.9 / 3.6	100	H388H (CAT→CAC)	thiI	tRNA sulfurtransferase
Reads supporting (aligned to +/- strand): ref base A (51/42); new base G (3/2); total (54/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.73e-01

Only 100 of 107 total aligned reads displayed.

GCATGGCCTGGACTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGC  >  NC_002947/5750313‑5750604

gCATGGCCTGGACTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGa                                                                                                                                                >  2:1752101/1‑150 (MQ=255)

 cATGGCCTGGACTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGAt                                                                                                                                               <  1:2319566/150‑1 (MQ=255)

  aTGGCCTGGACTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATg                                                                                                                                              >  2:1115952/1‑150 (MQ=255)

   tggactggacTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATgg                                                                                                                                             >  2:1125047/1‑150 (MQ=255)

   tGGCCTGGACTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATgg                                                                                                                                             >  2:435267/1‑150 (MQ=255)

   tGGCCTGGACTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATgg                                                                                                                                             <  2:1794127/150‑1 (MQ=255)

   tGGCCTGGACTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGAGCACATGATCGATgg                                                                                                                                             >  1:1075280/1‑150 (MQ=255)

     gCCTGGACTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCATGTGATCGATgagc                                                                                                                                           <  2:237683/150‑4 (MQ=255)

     gCCTGGACTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGaa                                                                                                                                           <  2:373903/150‑1 (MQ=255)

       cTGGACTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCATGTGATCGATg                                                                                                                                              >  1:1619408/1‑145 (MQ=255)

       cTGGACTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCATGTGATCGATg                                                                                                                                              <  2:1619408/145‑1 (MQ=255)

        tGGACTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATg                                                                                                                                        >  1:2312581/1‑150 (MQ=255)

           aCTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCGTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGGTCACATGACCGATGGAAGTGAAc                                                                                                                                     >  1:1351902/1‑150 (MQ=255)

             ttCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACtt                                                                                                                                   >  2:274352/1‑150 (MQ=255)

             ttCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGGGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACtt                                                                                                                                   >  1:2314346/1‑150 (MQ=255)

                    ccTTGCAGCACCGGAGGGTCGTCTTCCTGGGCATCGGGGGGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCGACGTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGACCGTGgcgcgc                                                                                                                            <  2:379228/150‑1 (MQ=255)

                    ccTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGgcgcgc                                                                                                                            <  2:2125558/150‑1 (MQ=255)

                          aGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAaga                                                                                                                      >  2:290209/1‑150 (MQ=255)

                           gCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAagag                                                                                                                     >  1:2144028/1‑150 (MQ=255)

                           gCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAagag                                                                                                                     >  2:1455055/1‑150 (MQ=255)

                              ccAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCaa                                                                                                                        <  2:130298/144‑1 (MQ=255)

                              ccAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCaa                                                                                                                        >  1:130298/1‑144 (MQ=255)

                                    gTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAgcg                                                                                                                   >  2:1954232/1‑143 (MQ=255)

                                    gTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCa                                                                                                            >  2:1965242/1‑150 (MQ=255)

                                    gTTCGTCTTCATGCGGTTTGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCCGCGGTTCGGCGATGTGTTCTATTTAGCCGTCCTTGGCTCGCTAATCGCCCCCATCATTTGACGAAATTAACTTGGCGCGCTCAAGAGCGCGCTCCa                                                                                                            <  1:1125047/150‑1 (MQ=255)

                                    gTTCGTCTGCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAgcg                                                                                                                   <  1:1954232/143‑1 (MQ=255)

                                     ttCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGCTCTGGCCTGGCAGCGCCTCGGCCACTTCCTCTATTTCGATGTCCTTGCCCAGCTCATCGAGCACCTGATCGATGGATATTAACTTGGCGCGCTCAAGAGCGCGCTCCAg                                                                                                           <  1:1761855/150‑1 (MQ=255)

                                     ttCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTCAGCAATGGTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCGCAAGAGCGCGCTCCAg                                                                                                           >  1:42632/1‑150 (MQ=255)

                                         tCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACg                                                                                                       <  2:2084184/150‑1 (MQ=255)

                                            tCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGcc                                                                                                    <  2:2245462/150‑1 (MQ=255)

                                                gggCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGt                                                                                                >  1:254680/1‑150 (MQ=255)

                                                    aTCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATg                                                                                                                                              >  1:304081/1‑100 (MQ=255)

                                                    aTCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATg                                                                                                                                              <  2:304081/100‑1 (MQ=255)

                                                    aTCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAaga                                                                                                                      >  1:1444682/1‑124 (MQ=255)

                                                    aTCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAaga                                                                                                                      <  2:1444682/124‑1 (MQ=255)

                                                        tggTGCCCAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGc                                                                                        <  2:391402/149‑1 (MQ=255)

                                                          gTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCtt                                                                                      <  2:1075280/150‑1 (MQ=255)

                                                               gAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGCGCGCGCTCCAGCACGGCCATGTCGAACGGCTTttctt                                                                                 >  1:2451936/1‑150 (MQ=255)

                                                               gAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTttctt                                                                                 <  2:2314346/150‑1 (MQ=255)

                                                               gAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTttctt                                                                                 >  2:2362477/1‑150 (MQ=255)

                                                               gAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTttctt                                                                                 >  1:2387101/1‑150 (MQ=255)

                                                                aaTGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTttcttc                                                                                <  1:2406172/150‑1 (MQ=255)

                                                                aaTGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTttcttc                                                                                <  1:1734728/150‑1 (MQ=255)

                                                                    tCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGc                                                                            >  2:388906/1‑150 (MQ=255)

                                                                       aTGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTcc                                                                         >  1:2172769/1‑150 (MQ=255)

                                                                            gATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCg                                                                    >  1:779890/1‑150 (MQ=255)

                                                                            gATCTGGCCTGGCAGCGCCCAGGCCAATTCCCCGATTAAAATGTCCTGGCCCAGCTCATCGATCACATGATCGATGGAAAGGAACTTGGCTCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCg                                                                    >  2:2104459/1‑150 (MQ=255)

                                                                             aTCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGt                                                                              >  2:2125975/1‑139 (MQ=255)

                                                                             aTCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGt                                                                              <  1:2125975/139‑1 (MQ=255)

                                                                              tCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGt                                                                  <  2:2387101/150‑1 (MQ=255)

                                                                                tggcctggcAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAAATGCTTTTCTTCGTGCTCCATGCGGTGa                                                                <  1:1455055/150‑1 (MQ=255)

                                                                                 ggcctggcAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGAc                                                               <  2:2165602/150‑1 (MQ=255)

                                                                                  gcctggcAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACg                                                              >  2:1660319/1‑150 (MQ=255)

                                                                                  gcctggcAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACg                                                              >  2:266002/1‑150 (MQ=255)

                                                                                    ctggcAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGc                                                                                                     <  1:713910/109‑1 (MQ=255)

                                                                                    ctggcAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGc                                                                                                     >  2:713910/1‑109 (MQ=255)

                                                                                      ggcAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCCGGACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTg                                                          <  1:435267/150‑1 (MQ=255)

                                                                                      ggcAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTg                                                          <  2:42632/150‑1 (MQ=255)

                                                                                       gcAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTgg                                                         >  2:595962/1‑150 (MQ=255)

                                                                                          gcgcCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGc                                                                     >  1:291901/1‑135 (MQ=255)

                                                                                          gcgcCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGc                                                                     <  2:291901/135‑1 (MQ=255)

                                                                                          gcgcCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGTAt                                                      >  2:432373/1‑150 (MQ=255)

                                                                                              cTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCgg                                                                   <  2:335317/133‑1 (MQ=255)

                                                                                              cTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCgg                                                                   >  1:335317/1‑133 (MQ=255)

                                                                                                    cACTTCCTCTATTTCGATGTCCTTGCCCAGCTCATCGATCACCTCATAGATCACTATTAACTTTGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCgg                                            <  2:64959/150‑1 (MQ=255)

                                                                                                    cACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCgg                                            <  2:1268413/150‑1 (MQ=255)

                                                                                                      cTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGt                                          <  1:1965242/150‑1 (MQ=255)

                                                                                                      cTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGt                                          <  2:2172769/150‑1 (MQ=255)

                                                                                                       ttCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGtt                                         >  2:2140919/1‑150 (MQ=255)

                                                                                                          cTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTAc                                      >  1:1876423/1‑150 (MQ=255)

                                                                                                          cTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACACGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTAc                                      >  2:811134/1‑150 (MQ=255)

                                                                                                           tCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGGTGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCAGGGGGGGTCGGGTATAcc                                     >  2:1184541/1‑150 (MQ=255)

                                                                                                           tCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGgcgcgc                                                                                                                            <  1:52679/63‑1 (MQ=255)

                                                                                                           tCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGgcgcgc                                                                                                                            >  2:52679/1‑63 (MQ=255)

                                                                                                           tCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCAt                                                                       <  2:1508181/116‑1 (MQ=255)

                                                                                                           tCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCAt                                                                       >  1:1508181/1‑116 (MQ=255)

                                                                                                             gATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAg                                                                                                           <  1:280201/78‑1 (MQ=255)

                                                                                                             gATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAg                                                                                                           >  2:280201/1‑78 (MQ=255)

                                                                                                                        ccTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCgg                                            <  2:602176/130‑1 (MQ=255)

                                                                                                                        ccTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCgg                                            >  1:602176/1‑130 (MQ=255)

                                                                                                                         cTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCa                       <  2:539646/150‑1 (MQ=255)

                                                                                                                          ttGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAg                      <  1:388906/150‑1 (MQ=255)

                                                                                                                              ccAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTAtt                  >  1:2315762/1‑150 (MQ=255)

                                                                                                                               cAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTTCCCATGCTGTTCTTCGGTTGCCTGGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTc                 <  1:290209/150‑1 (MQ=255)

                                                                                                                                aGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGAATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTcc                <  1:266002/150‑1 (MQ=255)

                                                                                                                                 gCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCCCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGCTGTCTCTTGTCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCg               <  1:2275515/150‑1 (MQ=255)

                                                                                                                                     aTCGATCATGTGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTGCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGAGCACGCCGCAGTATTCCGGCAt           >  2:567067/1‑150 (MQ=255)

                                                                                                                                     aTCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCAt           >  2:738513/1‑150 (MQ=255)

                                                                                                                                     aTCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCAt           >  2:1025621/1‑150 (MQ=255)

                                                                                                                                     aTCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCAt           <  2:2312581/150‑1 (MQ=255)

                                                                                                                                     aTCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCAt           <  1:1684126/150‑1 (MQ=255)

                                                                                                                                       cGATCACATGATCGATGGAAATGAACTTGGCTCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCAtgt         <  1:2104459/150‑1 (MQ=255)

                                                                                                                                        gATCACATGATCGATGGAAATGAACTTTTCGCGCTGAAGGGCGCGCGCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCAtgtg        >  1:2264147/1‑150 (MQ=255)

                                                                                                                                        gATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCAtgtg        >  1:845961/1‑150 (MQ=255)

                                                                                                                                             caTGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGTATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTgg   <  1:432373/150‑1 (MQ=255)

                                                                                                                                             caTGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATgg                                                    >  1:2168209/1‑101 (MQ=255)

                                                                                                                                             caTGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATgg                                                    <  2:2168209/101‑1 (MQ=255)

                                                                                                                                             caTGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTgg   <  2:254680/150‑1 (MQ=255)

                                                                                                                                             caTGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTgg   >  2:2297461/1‑150 (MQ=255)

                                                                                                                                              aTGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGATTAACCGCGAGCTCGTATCTGCATTCCTGCATGGGCTTTGc  >  2:2358014/1‑150 (MQ=255)

GCATGGCCTGGACTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGC  >  NC_002947/5750313‑5750604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`