BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,857,759	G→T	5.4%	E84D (GAG→GAT)	PP_5134 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,857,759	0	G	T	5.4%	400.9 / 6.4	124	E84D (GAG→GAT)	PP_5134	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (57/60); new base T (5/2); total (62/62)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

Only 100 of 130 total aligned reads displayed.

GTCCCACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAGCCGGTGCTGGCCAA  >  NC_002947/5857612‑5857908

gtCCCACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAgcg                                                                                                                                                     <  1:2308311/150‑1 (MQ=255)

 tCCCACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAgcgc                                                                                                                                                    >  2:2102689/1‑150 (MQ=255)

 tCCCACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAgcgc                                                                                                                                                    >  1:760982/1‑150 (MQ=255)

  cccACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGcc                                                                                                                                                   >  1:1414135/1‑150 (MQ=255)

    cACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCGCGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTg                                                                                                                                                 >  2:605354/1‑150 (MQ=255)

    cACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTg                                                                                                                                                 >  2:1936098/1‑150 (MQ=255)

       cGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGCTCGCCTgg                                                                                                                                                <  2:671347/148‑1 (MQ=255)

         ccccAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACgg                                                                                                                                            >  2:494081/1‑150 (MQ=255)

           ccAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGcc                                                                                                                                          >  2:1238162/1‑150 (MQ=255)

            cAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGccc                                                                                                                                         >  2:1476028/1‑150 (MQ=255)

              gCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTg                                                                                                                                       >  1:1120289/1‑150 (MQ=255)

                  tactCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGTTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCg                                                                                                                                   >  2:930684/1‑150 (MQ=255)

                     tCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCt                                                                                                                                <  1:2059656/150‑1 (MQ=255)

                     tCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCt                                                                                                                                >  1:341064/1‑150 (MQ=255)

                               gAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGtttt                                                                                                                      >  1:1955159/1‑150 (MQ=255)

                                aaGGTGCGCGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTc                                                                                                                     <  1:605354/150‑1 (MQ=255)

                                 aGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGc                                                                                                                                 <  2:985247/137‑1 (MQ=255)

                                 aGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGc                                                                                                                                 >  1:985247/1‑137 (MQ=255)

                                    tGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCa                                                                                                                 <  1:1936098/150‑1 (MQ=255)

                                    tGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCa                                                                                                                 <  1:2131247/150‑1 (MQ=255)

                                    tGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCa                                                                                                                 <  2:403789/150‑1 (MQ=255)

                                    tGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCa                                                                                                                 <  2:1334606/150‑1 (MQ=255)

                                    tGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCa                                                                                                                 <  1:494081/150‑1 (MQ=255)

                                      cACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAgg                                                                                                               <  1:327496/150‑1 (MQ=255)

                                       aCGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAggg                                                                                                              >  2:1413179/1‑150 (MQ=255)

                                       aCGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAggg                                                                                                              >  1:1477461/1‑150 (MQ=255)

                                         ggCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCa                                                                                                            <  2:315068/150‑1 (MQ=255)

                                           cTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGtt                                                                                                                        <  2:981853/136‑1 (MQ=255)

                                           cTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGc                                                                                                          <  1:1908147/150‑1 (MQ=255)

                                              tCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGAGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGaa                                                                                                       <  2:1632697/150‑1 (MQ=255)

                                                    gACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAgcgcgc                                                                                                 <  2:760982/150‑1 (MQ=255)

                                                        gTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAAc                                                                                             >  1:53536/1‑150 (MQ=255)

                                                             ccTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTc                                                                                        <  1:1013603/150‑1 (MQ=255)

                                                             ccTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTc                                                                                        <  2:341064/150‑1 (MQ=255)

                                                               tttGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGa                                                                                      <  2:1477461/150‑1 (MQ=255)

                                                                  gCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCa                                                                                   <  1:1413179/150‑1 (MQ=255)

                                                                     ggAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCagga                                                                                <  2:338379/150‑1 (MQ=255)

                                                                      gAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCaggag                                                                               <  1:2325873/150‑1 (MQ=255)

                                                                        gCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAg                                                                                     >  1:392928/1‑142 (MQ=255)

                                                                        gCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAg                                                                                     <  2:392928/142‑1 (MQ=255)

                                                                         cTGTACGACACGCCCGGCCTGGAAGACGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCtt                                                                                         <  1:390597/137‑1 (MQ=255)

                                                                           gTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCag                                                                                  >  2:2424783/1‑142 (MQ=255)

                                                                           gTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCag                                                                                  <  1:2424783/142‑1 (MQ=255)

                                                                            tACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAg                                                                         >  2:972187/1‑150 (MQ=255)

                                                                            tACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAg                                                                         <  1:2102689/150‑1 (MQ=255)

                                                                            tACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAg                                                                         >  1:728591/1‑150 (MQ=255)

                                                                            tACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAg                                                                         <  1:1467989/150‑1 (MQ=255)

                                                                             aCGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAgg                                                                        <  2:1903148/150‑1 (MQ=255)

                                                                                 cacGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTAtt                                                                    >  1:629131/1‑150 (MQ=255)

                                                                                  acGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTg                                                                   <  2:1955159/150‑1 (MQ=255)

                                                                                   cGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTgc                                                                  <  1:1837622/150‑1 (MQ=255)

                                                                                     cccGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTgcgc                                                                <  2:1414135/150‑1 (MQ=255)

                                                                                      ccGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGcc                                                               >  1:2287742/1‑150 (MQ=255)

                                                                                         gCCTGGAAGATGCCATCGCCCTGCTCCACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGCGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAAc                                                            <  2:516108/150‑1 (MQ=255)

                                                                                            tGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAActgc                                                         >  2:610534/1‑150 (MQ=255)

                                                                                                 gATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGcc                                                    <  1:312849/150‑1 (MQ=255)

                                                                                                  aTGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGcc                                                                                                >  1:1219636/1‑105 (MQ=255)

                                                                                                  aTGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGcc                                                                                                <  2:1219636/105‑1 (MQ=255)

                                                                                                  aTGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCa                                                   <  1:972187/150‑1 (MQ=255)

                                                                                                  aTGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCa                                                   <  1:930684/150‑1 (MQ=255)

                                                                                                      cATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCaa                                                                          >  2:2381629/1‑123 (MQ=255)

                                                                                                      cATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCaa                                                                          <  1:2381629/123‑1 (MQ=255)

                                                                                                      cATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTgcgc                                                                >  2:2063991/1‑133 (MQ=255)

                                                                                                      cATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTgcgc                                                                <  1:2063991/133‑1 (MQ=255)

                                                                                                           cccTGCTCGACTACTTTGAATGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCTAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCg                                          <  2:153877/150‑1 (MQ=255)

                                                                                                               gCTCGGCTATCTCTACCGCCTGAGGCTC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCt                                      <  2:1319298/150‑1 (MQ=255)

                                                                                                               gCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCt                                      <  1:610534/150‑1 (MQ=255)

                                                                                                                cTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTg                                                                   <  1:998800/120‑1 (MQ=255)

                                                                                                                 tCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCAAACTGCTGGCCAGTCATGCCGGCCTGt                                    >  1:1000784/1‑150 (MQ=255)

                                                                                                                  cGACTCCCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCCGGGGAGCGCGCCTAGGCGTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTa                                   <  1:291876/150‑1 (MQ=255)

                                                                                                                     cTACCTTGAACGCCTGGAGCGC‑CCAGGCTATCGCCTTGACGGCCCTGCCCTGCTTGATCGTTTTCTGCATGGCAGCGAATCGCGCCAACGCTTCGAGCAGGAGGCCAAGTGATTGCGCCATCTGCTTGCCAGTCATGCCGGCCTTTAtgt                                <  2:1697361/150‑1 (MQ=255)

                                                                                                                         cTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATc                            >  1:571153/1‑150 (MQ=255)

                                                                                                                          ttGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCg                           >  1:1065625/1‑150 (MQ=255)

                                                                                                                           tGACCGACTGGATCGC‑CCAGGCGAGCGCCTGGACGGCACTGCCCGGCTGGGGCGTTTTCTGCAGGGCAGCGAGGGGTGCTAACGCTTCGAGCAGTAGGCCAAGGTATTGCACCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGa                          <  1:534430/150‑1 (MQ=255)

                                                                                                                           tGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGa                          <  2:2438393/150‑1 (MQ=255)

                                                                                                                            gAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAATTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGAt                         >  2:234274/1‑150 (MQ=255)

                                                                                                                            gAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGAt                         <  2:2137513/150‑1 (MQ=255)

                                                                                                                              tcGCCTGG‑GCGCTCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCa                                                                                   <  2:298004/89‑1 (MQ=255)

                                                                                                                              tcGCCTGG‑GCGCTCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCa                                                                                   >  1:298004/2‑90 (MQ=255)

                                                                                                                              tcGCCTGG‑GCGCTCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTAt                                  <  2:33024/138‑1 (MQ=255)

                                                                                                                              aCGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCAAGTCATGCCGGCCTGTATGTGATCGATgc                       <  1:2424648/150‑1 (MQ=255)

                                                                                                                               cGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATgcg                      >  1:1855075/1‑150 (MQ=255)

                                                                                                                                gCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATgcgc                     >  1:966316/1‑150 (MQ=255)

                                                                                                                                  cTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATgcgcgc                   >  1:1892745/1‑150 (MQ=255)

                                                                                                                                  cTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTGTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATgcgcgc                   >  2:454574/1‑150 (MQ=255)

                                                                                                                                   tGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTAt                                  >  1:1089957/1‑134 (MQ=255)

                                                                                                                                   tGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTAt                                  <  2:1089957/134‑1 (MQ=255)

                                                                                                                                       gccgtccAGGCGCTCGCCTGGGCGGCGGTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAGc               >  1:1637878/6‑150 (MQ=255)

                                                                                                                                        cgc‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAGCCg             <  1:1321943/150‑1 (MQ=255)

                                                                                                                                         gc‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCt                                      <  2:552537/124‑1 (MQ=255)

                                                                                                                                         cgtccAGGCGCTCGCCTGGGCGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAGCCg             >  2:1762973/4‑150 (MQ=255)

                                                                                                                                          gtccAGGCGCTCGCCTGGGCGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGCGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAGCCgg            >  2:203628/3‑150 (MQ=255)

                                                                                                                                          c‑ccAGGCGAGCGCCTGGACGGCCCTGCCCTTCTGGAGCGTTTTCTTCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAGCCGGt           >  1:1233895/1‑150 (MQ=255)

                                                                                                                                           tccAGGCGCTCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAGCCGGt           >  2:854354/2‑150 (MQ=255)

                                                                                                                                             cAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATc                            >  2:861325/1‑131 (MQ=255)

                                                                                                                                             cAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATc                            <  1:861325/131‑1 (MQ=255)

                                                                                                                                             cAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAGCCGGTGc         <  2:1120101/150‑1 (MQ=255)

                                                                                                                                              aGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAGCCGGTGCt        <  2:2287742/150‑1 (MQ=255)

                                                                                                                                                    gcgcCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAGCCGGTGCTGGCCaa  >  1:1632397/1‑150 (MQ=255)

                                                                                                                                                    gcgcCTGGACGGCCCTGCCCGGCTGGAGCGATTTCTGCAGCGCACCGGAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAGCCGGTGCTGGCCaa  >  2:1466105/1‑150 (MQ=255)

GTCCCACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAGCCGGTGCTGGCCAA  >  NC_002947/5857612‑5857908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`