Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,874,875 G→T 6.4% S451* (TCG→TAG) ‡ ilvA‑II ← threonine deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,874,8750GT 6.4% 295.5 / 7.2 93S451* (TCG→TAG) ‡ilvA‑IIthreonine deaminase
Reads supporting (aligned to +/- strand):  ref base G (45/42);  new base T (3/3);  total (48/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01

TGTAGGCCGGGTTTTCGGTCTCGTCCCAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGACGAATATGCAGCTTGGCCAATTCGTT  >  NC_002947/5874729‑5875023
                                                                                                                                                  |                                                                                                                                                    
tGTAGGCCGGGTTTTCGGTCTCGTCCCAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCgaga                                                                                                                                                   <  1:1112403/150‑1 (MQ=255)
   aGGCCGGGTTTTCGGTCTCGTCCCAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCTCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGt                                                                                                                                                >  1:1406003/1‑150 (MQ=255)
     gCCGGGTTTTCGGTCTCGTCCCAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTc                                                                                                                                              >  1:2352935/1‑150 (MQ=255)
     gCCGGGTTTTCGGTCTCGTCCCAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTc                                                                                                                                              >  1:2049632/1‑150 (MQ=255)
       cGGGTTTTCGGTCTCGTCCCAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCa                                                                                                                                            >  1:230213/1‑150 (MQ=255)
       cGGGTTTTCGGTCTCGTCCCAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCa                                                                                                                                            <  2:2035176/150‑1 (MQ=255)
                 gTCTCGTCCCAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGGCGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCgaga                                                                                                                                                   <  1:1090942/133‑1 (MQ=255)
                 gTCTCGTCCCAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGGCGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCgaga                                                                                                                                                   >  2:1090942/1‑133 (MQ=255)
                  tctcGTCCCAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATCCTTCGGGGAGTGGAACATCGAGATGTTCCAGCGCCCGCCCa                                                                                                                                 >  1:723687/1‑150 (MQ=255)
                    tcGTCCCAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCTCGATGTTCCa                                                                                                                                            <  2:1565539/137‑1 (MQ=255)
                    tcGTCCCAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCTCGATGTTCCa                                                                                                                                            >  1:1565539/1‑137 (MQ=255)
                      gTCCCAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCtt                                                                                                                             >  1:653634/1‑150 (MQ=255)
                      gTCCCAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGCTCCAGAGCCCGCCCAGCtt                                                                                                                             >  2:1635517/1‑150 (MQ=255)
                       tCCCAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCttg                                                                                                                            >  1:2095923/1‑150 (MQ=255)
                       tCCCAGTACGGGTAACCGATCTTAGGATAAGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCttg                                                                                                                            >  1:1882061/1‑150 (MQ=255)
                        cccAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCttgt                                                                                                                           <  1:1473624/150‑1 (MQ=255)
                         ccAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCttgtt                                                                                                                          <  1:2306795/150‑1 (MQ=255)
                         ccAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCttgtt                                                                                                                          >  2:301466/1‑150 (MQ=255)
                         ccAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCttgtt                                                                                                                          <  2:2122848/150‑1 (MQ=255)
                           aGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGa                                                                                                                        <  2:653634/150‑1 (MQ=255)
                                 ggTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGt                                                                                                                  <  2:230213/150‑1 (MQ=255)
                                  gTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGtt                                                                                                                 >  2:117012/1‑150 (MQ=255)
                                  gTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGtt                                                                                                                 >  1:2072864/1‑150 (MQ=255)
                                  gTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGtt                                                                                                                 <  1:198304/150‑1 (MQ=255)
                                   tAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTg                                                                                                                >  2:2057126/1‑150 (MQ=255)
                                       cGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGa                                                                                                                        <  1:1805044/138‑1 (MQ=255)
                                       cGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGa                                                                                                                        >  2:1805044/1‑138 (MQ=255)
                                        gATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAg                                                                                                           >  2:333086/1‑150 (MQ=255)
                                                ccAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGt                                                                                                                                                <  1:1204051/105‑1 (MQ=255)
                                                ccAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGc                                                                                                        >  2:1708400/1‑145 (MQ=255)
                                                ccAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGc                                                                                                        <  1:1708400/145‑1 (MQ=255)
                                                ccAGCGCTGCCGGCACCAGGTGACGTACGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGt                                                                                                                                                >  2:1204051/1‑105 (MQ=255)
                                                 cAGCGCTGCCGGCACCAGGTGCCGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTgg                                                                                                  <  2:897462/150‑1 (MQ=255)
                                                 cAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTgg                                                                                                  <  1:1443920/150‑1 (MQ=255)
                                                   gcgcTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGcc                                                                                                >  2:1586637/1‑150 (MQ=255)
                                                    cgcTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCg                                                                                               >  2:1770077/1‑150 (MQ=255)
                                                          cGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCCGCACCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCggg                                                                                         >  1:2299992/1‑150 (MQ=255)
                                                          cGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTCGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCggg                                                                                         <  2:1046179/150‑1 (MQ=255)
                                                          cGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCggg                                                                                         <  2:2072590/150‑1 (MQ=255)
                                                                cAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTc                                                                                   >  1:2056870/1‑150 (MQ=255)
                                                                   gTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGaa                                                                                <  1:117012/150‑1 (MQ=255)
                                                                   gTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGaa                                                                                <  1:301466/150‑1 (MQ=255)
                                                                   gTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTAGCCGCTCGGGGAATTCGaa                                                                                <  1:915938/150‑1 (MQ=255)
                                                                        gTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCACCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCgca                                                                           >  2:924517/1‑150 (MQ=255)
                                                                         ttCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGtt                                                                                                                 >  1:610840/1‑111 (MQ=255)
                                                                         ttCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGtt                                                                                                                 <  2:610840/111‑1 (MQ=255)
                                                                         ttCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCgcag                                                                          <  2:1482579/150‑1 (MQ=255)
                                                                             tcctccGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCAcc                                                                      >  1:1092443/1‑150 (MQ=255)
                                                                               ctccGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCAt                                                                    <  1:1492326/150‑1 (MQ=255)
                                                                               ctccGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCAt                                                                    >  2:1415566/1‑150 (MQ=255)
                                                                                tccGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCAtt                                                                   >  2:1066683/1‑150 (MQ=255)
                                                                                  cGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCGCTCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAAt                                                                                     <  1:1996094/130‑1 (MQ=255)
                                                                                  cGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAAt                                                                                     >  2:1996094/1‑130 (MQ=255)
                                                                                  cGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTtc                                                                 >  2:2260099/1‑150 (MQ=255)
                                                                                  cGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTtc                                                                 >  1:885595/1‑150 (MQ=255)
                                                                                    gCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGTCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTtcgt                                                               >  1:1822337/1‑150 (MQ=255)
                                                                                     cACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTtcgtc                                                              <  2:2106831/150‑1 (MQ=255)
                                                                                      aCCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGaa                                                                                                                    <  2:2323500/95‑1 (MQ=255)
                                                                                      aCCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGaa                                                                                                                    >  1:2323500/1‑95 (MQ=255)
                                                                                        cTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCGCCATTTCGTCGCt                                                           >  2:134320/1‑150 (MQ=255)
                                                                                        cTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCt                                                           >  1:1926883/1‑150 (MQ=255)
                                                                                        cTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCt                                                           <  2:1008835/150‑1 (MQ=255)
                                                                                        cTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCt                                                           >  1:2371086/1‑150 (MQ=255)
                                                                                         tGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCTCGATGTTcc                                                                                                                                             <  2:740152/67‑1 (MQ=39)
                                                                                         tGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCTCGATGTTcc                                                                                                                                             >  1:740152/1‑67 (MQ=39)
                                                                                               cccGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCg                                                    <  1:2383564/150‑1 (MQ=255)
                                                                                                ccGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCgg                                                   <  2:1926883/150‑1 (MQ=255)
                                                                                                ccGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGACCCTGGCCGCCCGGGGTATTCGAAGCGCATCACCATTGCGTCGCTGGCACCgg                                                   >  2:1840706/1‑150 (MQ=255)
                                                                                                    caccacGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGa                                               <  2:2374935/150‑1 (MQ=255)
                                                                                                       cacGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTg                                            <  1:1086483/150‑1 (MQ=255)
                                                                                                         cgcgCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGgc                                          <  1:2057126/150‑1 (MQ=255)
                                                                                                         cgcgCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGgc                                          <  2:885595/150‑1 (MQ=255)
                                                                                                               cGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTAGCCGCTCAGGGAATTCGAAGCGCAGCACCATTTCGTCGTTGACACCGGCCGAGTGGCCGCCGa                                    >  2:1212367/1‑150 (MQ=255)
                                                                                                                 tcggtcgcgCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGAcc                                  <  2:1450286/145‑1 (MQ=255)
                                                                                                                       gcgcCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGAGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGa                            <  1:1840706/150‑1 (MQ=255)
                                                                                                                          ccATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGACGa                         >  1:1793643/1‑150 (MQ=255)
                                                                                                                             tGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGACGAata                      <  2:1934440/150‑1 (MQ=255)
                                                                                                                               gTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCa                                                       <  2:2384979/115‑1 (MQ=255)
                                                                                                                               gTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCa                                                       >  1:2384979/1‑115 (MQ=255)
                                                                                                                                 tGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCACTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGACGAATATGCa                  <  1:333086/150‑1 (MQ=255)
                                                                                                                                    ggTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGACGAATATGCAGCt               >  1:984828/1‑150 (MQ=255)
                                                                                                                                    ggTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGACGAATATGCAGCt               >  2:1641339/1‑150 (MQ=255)
                                                                                                                                      tAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGACGAATATGCAGCTTg             <  2:1069950/150‑1 (MQ=255)
                                                                                                                                      tAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGACGAATATGCAGCTTg             <  2:1092443/150‑1 (MQ=255)
                                                                                                                                       gctGGAACATCTCGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGACGAATATGCAGCTTg             >  1:2066953/3‑149 (MQ=255)
                                                                                                                                       gctGGAACATCTCGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGACGAATATGCAGCTTg             <  2:2066953/147‑1 (MQ=255)
                                                                                                                                          ggAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGACGAATATGCAGCTTGGCCa         >  2:2186300/1‑150 (MQ=255)
                                                                                                                                           gAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGACGAATATGCAGCTTGGCCaa        >  2:1659757/1‑150 (MQ=255)
                                                                                                                                             aCATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCa                                                       >  1:1432138/1‑101 (MQ=255)
                                                                                                                                             aCATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCa                                                       <  2:1432138/101‑1 (MQ=255)
                                                                                                                                             aCATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCa                                                       <  2:1432127/101‑1 (MQ=255)
                                                                                                                                             aCATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCa                                                       >  1:1432127/1‑101 (MQ=255)
                                                                                                                                              cATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGACGAATATGCAGCTTGGCCAATTc     <  1:1586637/150‑1 (MQ=255)
                                                                                                                                               aTCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGACGAATATGCAGCTTGGCCAATTCg    <  1:1415566/150‑1 (MQ=255)
                                                                                                                                               aTCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGACGAATATGCAGCTTGGCCAATTCg    >  2:1964381/1‑150 (MQ=255)
                                                                                                                                                 cGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGACGAATATGCAGCTTGGCCAATTCGtt  <  2:2095923/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TGTAGGCCGGGTTTTCGGTCTCGTCCCAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGACGAATATGCAGCTTGGCCAATTCGTT  >  NC_002947/5874729‑5875023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑