Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,963,902 T→G 8.2% Y216D (TAT→GAT)  PP_5229 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,963,9020TG 8.2% 352.9 / 17.4 127Y216D (TAT→GAT) PP_5229hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (59/57);  new base G (9/2);  total (68/59)
Fisher's exact test for biased strand distribution p-value = 6.12e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

TGGCGTTCCTGTTCGGTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCT‑TGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGCTACCGTCGC  >  NC_002947/5963753‑5964050
                                                                                                                                                      |                                                                                                                                                    
    gTTCCTGTTCGGTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGNCGTGNCAGCTCT‑TGATTACCAGGGGCTGCATGGGGATCTGGCCATCGGCAGCCGCGACCCGCCACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCtataa                                                                                                                                                  >  1:198490/1‑149 (MQ=255)
    gTTCCTGTTCGGTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCT‑TGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTctat                                                                                                                                                  >  1:719945/1‑150 (MQ=255)
    gTTCCTGTTCGGTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCT‑TGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTatat                                                                                                                                                  >  1:1507918/1‑150 (MQ=255)
     ttCCTGTTCGGTGAAGAACAGCGCCGGTAAGTAGGCTCCAGCGCCGTGGCAGCTCT‑TGAGTACCAGGGGCTGCATGGTGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATc                                                                                                                                                 <  1:1137627/150‑1 (MQ=255)
     ttCCTGTTCGGTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCT‑TGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATc                                                                                                                                                 <  1:1382511/150‑1 (MQ=255)
         tgtgCGGTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCT‑TGAGTACCAGGGGCGGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCGATATCGCCg                                                                                                                                             >  2:384548/1‑150 (MQ=255)
          gTTCGGTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCT‑TGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGa                                                                                                                                            <  1:2118713/150‑1 (MQ=255)
           ttCGGTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCT‑TGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAg                                                                                                                                           <  1:1886260/150‑1 (MQ=255)
           ttCGGTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCT‑TGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAg                                                                                                                                           >  2:2192872/1‑150 (MQ=255)
               gTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCT‑TGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCTGTACCCTTTTCCTCGGCTATATCGCCGa                                                                                                                                            <  1:139788/145‑1 (MQ=255)
                tGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCT‑TGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCt                                                                                                                                      <  1:1071282/150‑1 (MQ=255)
                tGAAGAACAGCGCCGGGAAGTAGGCTCCAGAGCCGTGGCAGCTCT‑TGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCt                                                                                                                                      >  1:312109/1‑150 (MQ=255)
                  aagaaCAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCT‑TGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTg                                                                                                                                    <  1:1524914/150‑1 (MQ=255)
                  aagaaCAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCT‑TGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTg                                                                                                                                    <  1:681550/150‑1 (MQ=255)
                  aagaaCAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCT‑TGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTg                                                                                                                                    <  2:915560/150‑1 (MQ=255)
                   agaaCAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCCG‑TCTGTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTgg                                                                                                                                   <  1:1842645/150‑1 (MQ=255)
                            ccGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCT‑TGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTtgt                                                                                                                          >  2:252958/1‑150 (MQ=255)
                                gAAGTAGGCTCCAGCGCCGTGGCAGCTCT‑TGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCa                                                                                                                      <  2:729993/150‑1 (MQ=255)
                                       gCTCCAGCGCCGTGGCAGCTCT‑TGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCa                                                                                                               >  2:2361375/1‑150 (MQ=255)
                                       gCTCCAGCGCCGTGGCAGCTCT‑TGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCa                                                                                                               >  2:286605/1‑150 (MQ=255)
                                         tCCAGCGCCGTGGCAGCTCT‑TGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTtgt                                                                                                                          >  1:1016278/1‑137 (MQ=255)
                                         tCCAGCGCCGTGGCAGCTCT‑TGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTtgt                                                                                                                          <  2:1016278/137‑1 (MQ=255)
                                                ccGTGGCAGCTCT‑TGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGAccc                                                                                                      <  2:2132418/150‑1 (MQ=255)
                                                 ggTGGCAGATCT‑TGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGAAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGAccat                                                                                                     <  2:1310008/149‑3 (MQ=255)
                                                   tGGCAGCTCT‑TGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGATACATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTgc                                                                                                   <  1:1995780/150‑1 (MQ=255)
                                                     gCAGCTCT‑TGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTgcgc                                                                                                 >  2:1948984/1‑150 (MQ=255)
                                                          tct‑TGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCAcgc                                                                                                                   <  1:1535668/127‑1 (MQ=255)
                                                               gAGTACCAGGGGCTGCATGGGGTTCTAGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGAc                                                                                                        >  1:673520/1‑134 (MQ=255)
                                                               gAGTACCAGGGGCTGCATGGGGTTCTAGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGAc                                                                                                        <  2:673520/134‑1 (MQ=255)
                                                                  tACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCtga                                                                                     <  1:2361375/150‑1 (MQ=255)
                                                                   aCCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCtgat                                                                                    <  1:1418883/150‑1 (MQ=255)
                                                                    ccAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCtgatg                                                                                   >  1:1286059/1‑150 (MQ=255)
                                                                        gggCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAAc                                                                               >  2:1745722/1‑150 (MQ=255)
                                                                         ggCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGANACg                                                                              <  2:1507918/150‑1 (MQ=255)
                                                                         ggCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACg                                                                              >  2:1845460/1‑150 (MQ=255)
                                                                          gCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGc                                                                             >  2:2295176/1‑150 (MQ=255)
                                                                            tGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCa                                                                           <  1:2295176/150‑1 (MQ=255)
                                                                             gCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTgc                                                                                                   <  1:2406856/125‑1 (MQ=255)
                                                                             gCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTgc                                                                                                   >  2:2406856/1‑125 (MQ=255)
                                                                               aTGNGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCt                                                                        >  2:1512807/1‑150 (MQ=255)
                                                                               aTGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAg                                                                          <  1:1279354/148‑1 (MQ=255)
                                                                                 ggggTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTgg                                                                      >  1:735767/1‑150 (MQ=255)
                                                                                    gTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAgg                                                                   >  2:1680682/1‑150 (MQ=255)
                                                                                    gTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAgg                                                                   <  2:41892/150‑1 (MQ=255)
                                                                                        tGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACg                                                                                         >  1:2064873/1‑124 (MQ=255)
                                                                                        tGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACg                                                                                         <  2:2064873/124‑1 (MQ=255)
                                                                                        tGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTa                                                               >  2:369991/1‑150 (MQ=255)
                                                                                          gCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCAcgc                                                                                                                   <  1:1386703/96‑1 (MQ=255)
                                                                                          gCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCAcgc                                                                                                                   >  2:1386703/1‑96 (MQ=255)
                                                                                          gCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTAtt                                                             >  2:582775/1‑150 (MQ=255)
                                                                                           ccATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTg                                                            >  1:129743/1‑150 (MQ=255)
                                                                                            cATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTAt                                                              <  1:810962/147‑1 (MQ=255)
                                                                                            cATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTAt                                                              >  2:810962/1‑147 (MQ=255)
                                                                                            cATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACACCAGCTGTAGGCGTTTTgc                                                           >  2:1722415/1‑150 (MQ=255)
                                                                                              tCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTgcga                                                         <  2:735767/150‑2 (MQ=255)
                                                                                                          aCCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCaa                                             >  1:947298/1‑150 (MQ=255)
                                                                                                           cccGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAAc                                            >  1:1990635/1‑150 (MQ=255)
                                                                                                            ccGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTa                                                               >  2:441083/1‑130 (MQ=255)
                                                                                                            ccGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTa                                                               <  1:441083/130‑1 (MQ=255)
                                                                                                             cGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGc                                                                  <  2:1430789/126‑1 (MQ=255)
                                                                                                             cGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGa                                          <  2:947298/150‑1 (MQ=255)
                                                                                                              gCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAg                                         >  2:1729496/1‑150 (MQ=255)
                                                                                                               cAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAgc                                        <  1:2207461/150‑1 (MQ=255)
                                                                                                                   acTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCa                                    <  1:252958/150‑1 (MQ=255)
                                                                                                                    cTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAg                                   <  1:1845460/150‑1 (MQ=255)
                                                                                                                     tACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAgg                                  >  2:1587171/1‑150 (MQ=255)
                                                                                                                     tACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAgg                                  >  1:750581/1‑150 (MQ=255)
                                                                                                                        aaGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAAc                                                                               >  1:904533/1‑102 (MQ=255)
                                                                                                                        aaGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAAc                                                                               <  2:904533/102‑1 (MQ=255)
                                                                                                                        aaGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGgtgt                               >  2:1889991/1‑150 (MQ=255)
                                                                                                                          gagCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCg                             <  2:2201847/150‑1 (MQ=255)
                                                                                                                            gcagcGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGa                           <  1:1680682/150‑1 (MQ=255)
                                                                                                                            gcagcGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGa                           <  2:129743/150‑1 (MQ=255)
                                                                                                                                  tCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCacac                     <  1:2192872/150‑1 (MQ=255)
                                                                                                                                     gTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTc                  >  1:1687443/1‑150 (MQ=255)
                                                                                                                                      tACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGcac                       <  2:1569004/144‑1 (MQ=255)
                                                                                                                                         ccTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAg              >  2:852915/1‑150 (MQ=255)
                                                                                                                                         ccTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAg              >  2:255494/1‑150 (MQ=255)
                                                                                                                                         ccTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCGGGAGGCTTATTGCGGCCACCTTCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAg              >  1:367310/1‑150 (MQ=255)
                                                                                                                                         caagcaccTCGGCGATATAGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAg              >  2:1370344/7‑150 (MQ=255)
                                                                                                                                          cTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAgg             >  2:770115/1‑150 (MQ=255)
                                                                                                                                           ttttCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAg                                   >  1:2280708/1‑127 (MQ=255)
                                                                                                                                           ttttCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAg                                   <  2:2280708/127‑1 (MQ=255)
                                                                                                                                           ttttCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAggg            <  1:892697/150‑1 (MQ=255)
                                                                                                                                             caccTCGGCGATATAGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGCt          >  1:925452/3‑150 (MQ=255)
                                                                                                                                              accTCGGCGATATAGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGc                                                                  >  1:374685/2‑93 (MQ=255)
                                                                                                                                              accTCGGCGATATAGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGc                                                                  <  2:374685/92‑1 (MQ=255)
                                                                                                                                                cTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCa               <  2:124671/142‑1 (MQ=255)
                                                                                                                                                cTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGAAACGAGCGCCAGGTGTCGGAGCACACCTCGCa               >  1:124671/1‑142 (MQ=255)
                                                                                                                                                cTCGGCTATATCGCCGAGGTGCGTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGCTAcc       >  1:2026690/1‑150 (MQ=255)
                                                                                                                                                cTCGGCGATATAGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAgg             <  2:1674015/144‑1 (MQ=255)
                                                                                                                                                cTCGGCGATATAGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAgg             >  1:1674015/1‑144 (MQ=255)
                                                                                                                                                cTCGGCGATATAGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGCTAcc       >  2:1857053/1‑150 (MQ=255)
                                                                                                                                                cTCGGCGATATAGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGCTAcc       >  2:1595533/1‑150 (MQ=255)
                                                                                                                                                 tCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCg                                                                                              >  1:1677894/1‑62 (MQ=255)
                                                                                                                                                 tCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGgt                                 >  1:2241687/1‑123 (MQ=255)
                                                                                                                                                 tCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGgt                                 <  2:2241687/123‑1 (MQ=255)
                                                                                                                                                 tCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGgt                                 >  1:987777/1‑123 (MQ=255)
                                                                                                                                                 tCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCAGTGAGGCGGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGCTACCg      >  2:324835/1‑150 (MQ=255)
                                                                                                                                                   ggCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGCTACCGTc    <  2:750581/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
TGGCGTTCCTGTTCGGTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCT‑TGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGCTACCGTCGC  >  NC_002947/5963753‑5964050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑