Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,027,955 A→T 13.2% intergenic (‑43/+68) PP_5277 ← / ← kauB MFS transporter/4‑guanidinobutyraldehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,027,9550AT13.2% 96.8 / 8.2 38intergenic (‑43/+68)PP_5277/kauBMFS transporter/4‑guanidinobutyraldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (9/24);  new base T (2/3);  total (11/27)
Fisher's exact test for biased strand distribution p-value = 6.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.00e-01

AGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACACTGAGCACCGATGCCAGTACGGCGAAGTAGGTACCCCAACGCATTACAGCCTCCCAGCCAATATTTCCAGGGGCCCATTTGCGGGCAAGCCCGCTCCTGCAACGGTCGCGAAGACCTGCAGGAGCGGGCTTGCCCGCGAAAAGGACCTACCGGGTTACAGCTTGATCCAGGTCGCCTTCAGCTCGGTGTACTTCTCCAGTGCGTGCAGCGACTTGTCAC  >  NC_002947/6027807‑6028087
                                                                                                                                                    |                                                                                                                                    
aGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACACTGAGCACCGATGCCAGTACGGCGAAGTAGGTACCCCAACGCATTACAGCCTCCCAGCCAATATTTCCAGGGGCCCATTTGCGGGCaa                                                                                                                                     <  2:880029/150‑1 (MQ=255)
    ccAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACACTGAGCACCGATGCCAGTACGGCGAAGTAGGTACCCCAACGCATTACAGCCTCCCAGCCAATATTTCCAGGGGCCCATTTGCGGGCAAGccc                                                                                                                                 >  2:132900/1‑150 (MQ=255)
     cAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACACTGAGCACCGATGCCAGTACGGCGAAGTAGGTACCCCAACGCATTACAGCCTCCCAGCCAATATTTCCAGGGGCCCATTTGCGGGCAAGCCCg                                                                                                                                <  1:132900/150‑1 (MQ=255)
        gCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACACTGAGCACCGATGCCAGTACGGCGAAGTAGGTACCCCAACGCATTACAGCCTCCCAGCCAATATTTCCAGGGGCCCATTTGCGGGCAAGCCCGCTc                                                                                                                             <  2:2389563/150‑1 (MQ=255)
        gCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACACTGAGCACCGATGCCAGTACGGCGAAGTAGGTACCCCAACGCATTACAGCCTCCCAGCCAATATTTCCAGGGGCCCATTTGCGGGCAAGCCCGCTc                                                                                                                             <  1:941711/150‑1 (MQ=255)
        gCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACACTGAGCACCGATGCCAGTACGGCGAAGTAGGTACCCCAACGCATTACAGCCTCCCAGCCAATATTTCCAGGGGCCCATTTGCGGGCAAGCCCGCTc                                                                                                                             <  1:2420335/150‑1 (MQ=255)
           tCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACACTGAGCACCGATGCCAGTACGGCGAAGTAGGTACCCCAACGCATTACAGCCTCCCAGCCAATATTTCCAGGGGCCCATTTGCGGGCTTGCCCGcaaatg                                                                                                                          <  1:27365/150‑6 (MQ=255)
               ggCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCTACACTGAGCACCGATGCCAGTACGGCGAAGTAGGTACCCCAACGCATTACAGCCTCCCAGCCAATATTTCCAGGGGCCCATTTGCGGGCAAGCCCGCTCCTGCAAc                                                                                                                      <  1:327271/150‑1 (MQ=255)
                gcgcAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACACTGAGCACCGATGCCAGTACGGCGAAGTAGGTACCCCAACGCATTACAGCCTCCCAGCCAATATTTCCAGGGGCCCATTTGCGGGCAAGCCCGCTCCTGCAACg                                                                                                                     <  2:1010060/150‑1 (MQ=255)
                   cAGGTACACCAGCTGCATGCTCACGCCAAGCGCCAGCCCGACACTGAGCACCGATGCCAGTACGGCGACAACGATTCTCCAACGCATTTCATCCTCCCAGCCAATATGTCCAGGGGCCCATTTGCGGGCAAGCCCGCTCCTGCAACGGTc                                                                                                                  <  1:757468/150‑1 (MQ=255)
                     gggACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACACTGAGCACCGATGCCAGTACGGCGAAGTAGGTACCCCAACGCATTACAGCCTCCCAGCCAATATTTCCAGGGGCCCATTTGCGGGCAAGCCCGCTCCTGCAACGGTcgc                                                                                                                <  2:1666812/150‑1 (MQ=255)
                     gggACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACACTGAGCACCGATGCCAGTACGGCGAAGTAGGTACCCCAACGCATTACAGCCTCCCAGCCAATATTCACCGGGGCCCATTTGCGGGCAAGCCCGCTCCTGCAACGGTcgc                                                                                                                <  2:1664166/150‑1 (MQ=255)
                          acCAGCGGCATGCTCACGCCAAGCTCCAGCCCGACACTGAGCACCGATGCCAGTACGGCGAAGTAGGTACCCCAACGCATTACAGCCTCCCAGCCAATATTTCCAGGGGCCCATTTGCGGGCAAGCCCGCTCCTGCAACGGTCGCGAAGa                                                                                                           <  1:90683/150‑1 (MQ=255)
                           ccAGCGGCATGCTCACGCCAAGCGCCAGCCCGACACTGAGCACCGATGCCAGTACGGCGAAGTAGGTACCCCAACGCATTACAGCCTCCCAGCCAATATTTCCAGGGGCCCATTTGCGGGCAAGCCCGCTCCTGCAACGGTCGCGAAGAc                                                                                                          >  1:2235362/1‑150 (MQ=255)
                               cGGCATGCTCACGCCAAGCGCCAGCCCGACACTGAGCACCGATGCCAGTACGGCGAAGTAGGTACCCCAACGCATTACAGCCTCCCAGCCAATATTTCCAGGGGCCCATTTGCGGGCAAGCCCGCTCCTGCAACGGTCGCGAAGACCTGc                                                                                                      <  1:1303661/150‑1 (MQ=255)
                                     gCTCACGCCAAGCGCCAGCCCGACACTGAGCACCGATGCCAGTACGGCGAAGTAGGTACCCCAACGCATTACAGCCTCCCAGCCAATATTTCCAGGGGCCCATTTGCGGGCAAGCCCGCTCCTGCAACGGTCGCGAAGACCTGCAGGAGc                                                                                                <  2:2062237/150‑1 (MQ=255)
                                     gCTCACGCCAAGCGCCAGCCCGACACTAAGCACCGATGCCAGTACGGCGAAGTAGGTACCCCAACGCATTACAGCCTCCCAGCCAATATTTCCAGGGGCCCATTTGCGGGCAAGCCCGCTCCTGCAACGGTCGCGAAGACCTGCAGGAGc                                                                                                <  2:570044/150‑1 (MQ=255)
                                     gCTCACCCCAAGTCCCAGCCCGACACTGAGCACCGATGCCAGTACGGCGAAGTAGATACCCTACTACATTATGTCCCCACCGCCAATATTTCCCGGGGCCCCTTTGCGGGCAAGCCCCCTCCTGCAACGGTCGCGAAGACCTGCAGGAGc                                                                                                <  1:1355577/150‑1 (MQ=255)
                                       tCACGCCAAGCGCCAGCCCGACACTGAGCACCGATGCCAGTACGGCGAAGTAGGTACCCCAACGCATTACAGCCTCCCAGCCAATATTTCCAGGGGCCCATTTGCGGGCTTGCCCGcaaatg                                                                                                                          >  2:1351675/1‑117 (MQ=255)
                                       tCACGCCAAGCGCCAGCCCGACACTGAGCACCGATGCCAGTACGGCGAAGTAGGTACCCCAACGCATTACAGCCTCCCAGCCAATATTTCCAGGGGCCCATTTGCGGGCTTGCCCGcaaatg                                                                                                                          <  1:1351675/122‑6 (MQ=255)
                                       tCACGCCAAGCGCCAGCCCGACACTGAGCACCGATGCCAGTACGGCGAAGTAGGTACCCCAACGCATTACAGCCTCCCAGCCAATATTTCCAGGGGCCCATTTGCGGGCAAGCCCGCTCCTGCAACGGTCGCGAAGACCTGCAGGAGCgg                                                                                              <  1:1896505/150‑1 (MQ=255)
                                                         cGACACTGAGCACCGATGCCAGTACGGCGAAGTAGGTACCCCAACGCATTACAGCCTCCCAGCCAATATTTCCAGGGGCCCATTTGCGGGCTTGCCCGcaaatg                                                                                                                          >  1:1625904/1‑99 (MQ=255)
                                                         cGACACTGAGCACCGATGCCAGTACGGCGAAGTAGGTACCCCAACGCATTACAGCCTCCCAGCCAATATTTCCAGGGGCCCATTTGCGGGCTTGCCCGcaaatg                                                                                                                          <  2:1625904/104‑6 (MQ=255)
                                                                          gCCAATACGGCGAAGAAGGTACCCCACCGCATTACAGCCTCCCAGCCAATATTTCCAGGGGCCCATTTGCGGGCAAGCCCGCTCCTGCAACGGTCGCGAAGACCTGCAGGAGCGGGCTTGCCCGCGAAAAGGACCTACCGGGTTACAGCt                                                           <  1:1551321/150‑1 (MQ=255)
                                                                                  ggCGAAGTAGGTACCCCAACGCATTACAGCCTCCCAGCCAATATTTCCAGGGGCCCATTTGCGGGCAAGCCCGCTCCTGCAACGGTCGCGAAGACCTGCAGGAGCGGGCTTGCCCGCGAAAAGGACCTACCGGGTTACAGCTTGATCCAg                                                   >  1:978079/1‑150 (MQ=255)
                                                                                  ggCGAAGTAGGTACCCCAACGCATTACAGCCTCCCAGCCAATATTTCCAGGGGCCCATTTGCGGGCAAGCCCGCTCCTGCAACGGTCGCGAAGACCTGCAGGAGCGGGCTTGCCCGCGAAAAGGACCTACCGGGTTACAGCTTGATCCAg                                                   <  2:2383445/150‑1 (MQ=255)
                                                                                  ggCGAAGTAGGTACCCCAACGCATTACAGCCTCCCAGCCAATATTTCCAGGGGCCCATTTGCGGGCAAGCCCGCTCCTGCAACGGTCGCGAAGACCTGCAGGAGCGGGCTTGCCCGCGAAAAGGACCTACCGGGTTACAGCTTGATCCAg                                                   >  2:1435435/1‑150 (MQ=255)
                                                                                           ggTACCCCAACGCATTACAGCCTCCCAGCCAATATTTCCAGGGGCCCATTTGCGGGCAAGCCCGCACCTGCAACGGTCGCGAAGACCTGCACGAACGGGCTTGCCCGCGAAAAGGACCTACCTGTTGACACCTCTATCCAGGTCGCCtgc                                          >  2:1134314/1‑148 (MQ=255)
                                                                                                  cAACGCATTACAGCCTCCCAGCCAATATTTCCAGGGGCCCATTTGCGGGCAAGCCCGCTCCTGCAAc                                                                                                                      <  1:1221667/67‑1 (MQ=255)
                                                                                                  cAACGCATTACAGCCTCCCAGCCAATATTTCCAGGGGCCCATTTGCGGGCAAGCCCGCTCCTGCAAc                                                                                                                      >  2:1221667/1‑67 (MQ=255)
                                                                                                  cAACGCATTACAGCCTCCCAGCCAATATTTCCAGGGGCCCATTTGCGGGCAAGCCCGCTCCTGCAACGGTCGCGAAGACCTGCAGGAGCGGGCTTGCCCGCGAAAAGGACCTACCGGGTTACAGCTTGATc                                                      >  1:1085376/1‑131 (MQ=255)
                                                                                                  cAACGCATTACAGCCTCCCAGCCAATATTTCCAGGGGCCCATTTGCGGGCAAGCCCGCTCCTGCAACGGTCGCGAAGACCTGCAGGAGCGGGCTTGCCCGCGAAAAGGACCTACCGGGTTACAGCTTGATc                                                      <  2:1085376/131‑1 (MQ=255)
                                                                                                                    cAGCCAATCTTTCCAGGGGCCCATTTGCGGGCAAGCCCGCTCCTGCAACGGTCGCGAAGACCTGCAGGAGCGGGCTTGCCCGCGAAAAGGACCTACCGGGTTACAGCTTGATCCAGGTCGCCTTCAGCTCGGTGTACTTCTCCAgtgcgt                 <  1:468332/150‑1 (MQ=255)
                                                                                                                    cAGCCAATATTTCCAGGGGCCCATTTGCGGGCAAGCCCGCTCCTGCAACGGTCGCGAAGACCTGCAGGAGCGGGCTTGCCCGCGAAAAGGACCTACCGGGTTACAGCTTGATCCAGGTCGCCTTCAGCTCGGTGTACTTCTCCAgtgcgt                 <  1:468628/150‑1 (MQ=255)
                                                                                                                        cAATATTTCCAGGGGCCCATTTGCGGGCAAGCCCGCTCCTGCAACGGTCGCGAAGACCTGCAGGAGCGGGCTTGCCCGCGAAAAGGACCTACCGGGTTACAGCTTGATCCAGGTCGCCTTCAGCTCGGTGTACTTCTCCAGTGCGTGCAg             >  1:2055238/1‑150 (MQ=255)
                                                                                                                                   ggggCCCATTTGCGGGCAAGCCCGCTCCTGCAACGGTCGCGAAGTCCTGCAGGGGCGGGCTTGCCCGCGAAAAGGACCTACCGGGTTACAGCTTGATCCAGGTCGTCTGCAGCTCGGTGTACTTCTCCAGTGCGTGCAGCGACTTGTCAc  <  1:1134314/150‑1 (MQ=255)
                                                                                                                                   ggggCCCATTTGCGGGCAAGCCCGCTCCTGCAACGGTCGCGAAGACCTGCAGGAGCGGGCTTGCCCGCGAAAAGGACCTACCGGGTTACAGCTTGATCCAGGTCGCCTTCAGCTCGGTGTACTTCTCCAGTGCGTGCAGCGACTTGTCAc  <  2:2235362/150‑1 (MQ=255)
                                                                                                                                      gCCCATTTGCGGGCAAGCCCGCTCCTGCAACGGTCGCGAAGACCTGCAGGAGCggg                                                                                             >  1:2404712/1‑56 (MQ=255)
                                                                                                                                      gCCCATTTGCGGGCAAGCCCGCTCCTGCAACGGTCGCGAAGACCTGCAGGAGCggg                                                                                             <  2:2404712/56‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                    
AGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACACTGAGCACCGATGCCAGTACGGCGAAGTAGGTACCCCAACGCATTACAGCCTCCCAGCCAATATTTCCAGGGGCCCATTTGCGGGCAAGCCCGCTCCTGCAACGGTCGCGAAGACCTGCAGGAGCGGGCTTGCCCGCGAAAAGGACCTACCGGGTTACAGCTTGATCCAGGTCGCCTTCAGCTCGGTGTACTTCTCCAGTGCGTGCAGCGACTTGTCAC  >  NC_002947/6027807‑6028087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑