Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 72,283 T→A 5.1% D189V (GAC→GTC)  glyQ ← glycine‑tRNA ligase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294772,2830TA 5.1% 349.6 / 6.7 117D189V (GAC→GTC) glyQglycine‑tRNA ligase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (61/50);  new base A (3/3);  total (64/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01

Only 100 of 119 total aligned reads displayed.

CCAGCTTGATCAGGCGGTTGGCTTCGCTTTCGTAGAAATCGAACAGTTCGAACAGTTTGTCGACGTTTGCATGTTCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCACTCGATGCCGCCAACCTGCTGGAAGTAGGTGAACTGGGTGACTTCCATGCCGTT  >  NC_002947/72134‑72431
                                                                                                                                                     |                                                                                                                                                    
ccAGCTTGATCAGGCGGTTGGCTTCGCTTTCGTAGAAATCGAACAGTTCGAACAGTTTGTCGACGTTTGCATGTTCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGt                                                                                                                                                      >  1:1307106/1‑150 (MQ=255)
    tttGATCAGGCGGTTGGCTTCGCTTTCGTAGAAATCGAACAGTTCGAACAGTTTGTCGACGTTTGCATGTTCAAAGTTGTAGGTCGACTGCTCCACCTCTTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCgga                                                                                                                                                  <  1:83447/149‑2 (MQ=255)
    cTTGATCAGGCGGTTGGCTTCGCTTTCGTAGAAATCGAACAGTTCGAACAGTTTGTCGACGTTTGCATGTTCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGc                                                                                                                                                  <  1:737304/150‑1 (MQ=255)
      tGATCAGGCGGTTGGCTTCGCTTTCGTAGAAATCGAACAGTTCGAACAGTTTGTCGACGTTTGCATGTTCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCG‑ACGGCCCGttcggcaa                                                                                                                                               <  1:1766368/150‑8 (MQ=255)
          cAGGCGGTTGGCTTCGCTTTCGTAGAAATCGAACAGTTCGAACAGTTTGTCGACGTTTGCATGTTCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGACGGcccgttc                                                                                                                                            <  1:1210859/150‑5 (MQ=255)
                gatcgCTTCGATTTCGTAGCAATCGACCAGTTCGAACAGGTTGGCGACGTTGGCATGTTCAGAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTc                                                                                                                                      >  2:2232531/5‑150 (MQ=255)
                     cTTCGCTTTCGTAGAAATCGAACAGTTCGAACAGTTTGTCGACGTTTGCATGTTCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTaca                                                                                                                                 >  2:1420684/1‑150 (MQ=255)
                          cTTTCGTAGAAATCGAACAGTTCGAACAGTTTGTCGAGCCCGGTATGTTCAAAGTTGTAGGTCGACTGCTCCCCCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGaa                                                                                                                            <  1:23525/150‑1 (MQ=255)
                          cTTTCGTAGAAATCGAACAGTTCGAACAGTTTGTCGACGTTTGCATGTTCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGaa                                                                                                                            <  2:2080237/150‑1 (MQ=255)
                           tttCGTAGAAATCGAACAGTTCGAACAGTTTGTCGACGTTTGCATGTTCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTTGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAAt                                                                                                                           >  2:1954058/1‑150 (MQ=255)
                           tttCGTAGAAATCGAACAGTTCGAACAGTTTGTCGACGTTTGCATGTTCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAAt                                                                                                                           >  1:979227/1‑150 (MQ=255)
                           tttCGTAGAAATCGAACAGTTCGAACAGTTTGTCGACGTTTGCATGTTCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAAt                                                                                                                           >  2:121435/1‑150 (MQ=255)
                           tttCGTAGAAATCGAACAGTTCGAACAGTTTGTCGACGTTTGCATGTTCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAAt                                                                                                                           >  1:2424146/1‑150 (MQ=255)
                           tttCGTAGAAATCGAACAGTTCGAACAGTTTGTAGACGTTTGCATGTTCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAGCGGGCCGTCGGCCCACACCAGGTCGTACACCGAAt                                                                                                                           >  1:2053281/1‑150 (MQ=255)
                               gTAGAAATCGAACAGTTCGAACAGTTTGTCGACGTTTGCATGTTCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTc                                                                                                                                                     >  2:693670/1‑120 (MQ=255)
                               gTAGAAATCGAACAGTTCGAACAGTTTGTCGACGTTTGCATGTTCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGAc                                                                                                                       >  2:2127456/1‑150 (MQ=255)
                                    aaTCGAACAGTTCGAACAGTTTGTCGACGTTTGCATGTTCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCt                                                                                                                  <  2:1307106/150‑1 (MQ=255)
                                     aTCGAACAGTTCGAACAGTTTGTCGACGTTTGCATGTTCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCCGGTCGGTCACCGAATCGACGCCCTg                                                                                                                 >  1:722515/1‑150 (MQ=255)
                                       cgaacagttcgaacagttTGTCGACGTTTGCATGTTCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGa                                                                                                               >  2:786174/1‑150 (MQ=255)
                                         aacagttcgaacagttTGTCGACGTTTGCATGTTCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAGCGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATg                                                                                                             >  2:1973594/1‑150 (MQ=255)
                                         aacagttcgaacagttTGTCGACGTTTGCATGTTCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATg                                                                                                             >  2:1985103/1‑150 (MQ=255)
                                              ttcgaacagttTGTCGACGTTTGCATGTTCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACAt                                                                                                        <  2:1444043/150‑1 (MQ=255)
                                              ttcgaacagttTGTCGACGTTTGCATGTTCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACAt                                                                                                        >  1:2268820/1‑150 (MQ=255)
                                               tcgaacagttTGTCGACGTTTGCATGTTCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACAtt                                                                                                       <  1:1264961/150‑2 (MQ=255)
                                                    cagttTGTCGACGTTTGCATGTTCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGAc                                                                                                                       <  1:797887/129‑1 (MQ=255)
                                                    cagttTGTCGACGTTTGCATGTTCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAg                                                                                                  <  1:58306/150‑1 (MQ=255)
                                                     agttTGTCGACGTTTGCATGTTCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTAc                                                                                                          >  1:1351642/1‑141 (MQ=255)
                                                     agttTGTCGACGTTTGCATGTTCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTAc                                                                                                          <  2:1351642/141‑1 (MQ=255)
                                                             gACGTTTGCATGTTCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAg                                                                                         <  2:709210/150‑1 (MQ=255)
                                                                gTTTGCATGTTCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGcc                                                                                      >  1:1325346/1‑150 (MQ=255)
                                                                 tttGCATGTTCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCg                                                                                     >  1:141942/1‑150 (MQ=255)
                                                                    gCATGTTCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCNTAg                                                                                  <  2:1510816/150‑1 (MQ=255)
                                                                     cATGTTCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAgg                                                                                 >  2:1528569/1‑150 (MQ=255)
                                                                       tGTTCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTg                                                                               >  1:717056/1‑150 (MQ=255)
                                                                        gtacAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACAAGTCCCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACAGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGa                                                                              >  1:1877879/4‑150 (MQ=255)
                                                                         ttCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGc                                                                                                     >  2:2076472/1‑126 (MQ=255)
                                                                         ttCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGgcgc                                                                                              <  2:212017/133‑1 (MQ=255)
                                                                         ttCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGgcgc                                                                                              >  1:212017/1‑133 (MQ=255)
                                                                         ttCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAgg                                                                                 <  2:596395/146‑1 (MQ=255)
                                                                         ttCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAgg                                                                                 >  1:596395/1‑146 (MQ=255)
                                                                         ttCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGAt                                                                             >  1:1826491/1‑150 (MQ=255)
                                                                          tCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTa                                                                                                                                   >  1:2346666/1‑95 (MQ=255)
                                                                          tCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTa                                                                                                                                   <  2:2346666/95‑1 (MQ=255)
                                                                                ttGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCg                                                                      <  2:1125676/150‑1 (MQ=255)
                                                                                  gTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGc                                                                                                                                                  >  2:260382/1‑72 (MQ=255)
                                                                                  gTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGc                                                                                                                                                  <  1:260382/72‑1 (MQ=255)
                                                                                  gTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGt                                                                    >  2:555149/1‑150 (MQ=255)
                                                                                    aGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACAt                                                                                                        <  2:1465921/112‑1 (MQ=255)
                                                                                    aGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACAt                                                                                                        >  1:1465921/1‑112 (MQ=255)
                                                                                     ggTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGAc                                                                 >  1:908542/1‑150 (MQ=255)
                                                                                      gTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGAcc                                                                >  1:825457/1‑150 (MQ=255)
                                                                                       tCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCg                                                               >  1:2245927/1‑150 (MQ=255)
                                                                                        cGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCgg                                                              <  2:2268820/150‑1 (MQ=255)
                                                                                             gCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCg                                                                      >  2:807338/1‑137 (MQ=255)
                                                                                               tCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCAc                                                       >  1:1943107/1‑150 (MQ=255)
                                                                                                 cACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCACTc                                                     >  1:1080107/1‑150 (MQ=255)
                                                                                                 cACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACAGGGTAGCACTc                                                     >  1:2286511/1‑150 (MQ=255)
                                                                                                 cACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACAAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCACTc                                                     <  2:444441/150‑1 (MQ=255)
                                                                                                    cTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAGCGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCACTCGAt                                                  <  2:2053281/150‑1 (MQ=255)
                                                                                                    cTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCACTCGAt                                                  >  1:1383011/1‑150 (MQ=255)
                                                                                                     tCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCACTCGATg                                                 >  1:2430467/1‑150 (MQ=255)
                                                                                                           tggtggAACACGTCGCCATAGGTGACCTTGCCGAATGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCACTCGATGCCGCCa                                           >  1:498596/1‑150 (MQ=255)
                                                                                                             gtggAACACGTCGCCATAGGTGACCTTGCNGAACGGGCCGTCGGNCCACNCCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGAGTTCACTGGTGACCGGGTAGCACTCGATGCCGCCAAc                                         >  1:331832/1‑150 (MQ=255)
                                                                                                             gtggAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGa                                                                              <  1:649633/113‑1 (MQ=255)
                                                                                                                gAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCACTCGATGCCGCCAACctg                                      <  1:786174/150‑1 (MQ=255)
                                                                                                                gAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCACTCGATGCCGCCAACctg                                      >  2:840721/1‑150 (MQ=255)
                                                                                                                 aaCACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCACTCGATGCCGCCAACctgc                                     <  1:555149/150‑1 (MQ=255)
                                                                                                                  acacGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCACTCGATGCCGCCAACctgct                                    <  1:373973/150‑1 (MQ=255)
                                                                                                                   cacGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCACTCGATGCCGCCAACctgctg                                   <  2:721452/150‑1 (MQ=255)
                                                                                                                    acGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCACTCGATGCCGCCAACCTGCTgg                                  <  1:840721/150‑1 (MQ=255)
                                                                                                                     cGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCACTCGATGCCGCCAACCTGCTGGa                                 >  1:1170533/1‑150 (MQ=255)
                                                                                                                      gTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCGGGTCGTACACCGAATCGACGCCCTGGATGTACATGCCCAGGCGCTCCAGGCCGTAGGTGATTTCACTAGTCGTTTTATAGCACTCGATGCCGCCAACCTGCTGGat                                <  1:1774245/150‑2 (MQ=255)
                                                                                                                      gTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAg                                                                                                  >  2:1893378/1‑84 (MQ=255)
                                                                                                                      gTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAg                                                                                                  <  1:1893378/84‑1 (MQ=255)
                                                                                                                      gTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGGCCGGGTa                                                           >  1:1962454/1‑123 (MQ=255)
                                                                                                                      gTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCACTCGATGCCGCCAACCTGCTGGaa                                >  1:1737347/1‑150 (MQ=255)
                                                                                                                      gTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCACTCGATGCCGCCAACCTGCTGGaa                                <  1:121435/150‑1 (MQ=255)
                                                                                                                         gCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCACTCGATGCCGCCAACCTGCTGGAAGTa                             <  2:1737347/150‑1 (MQ=255)
                                                                                                                          ccATGGGTTACCTCGCCTAACGGGTCGTCTGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCAGAAGGATATTGCACTGGTGACCGGGTAGCACTCGATGTCGCCAACCTGCTGGAAGTAg                            <  2:331832/150‑1 (MQ=255)
                                                                                                                          ccATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCACTCGATGCCGCCAACCTGCTGGAAGTAg                            <  2:1080107/150‑1 (MQ=255)
                                                                                                                          ccATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCACTCGATGCCGCCAACCTGCTGGAAGTAg                            <  2:2430467/150‑1 (MQ=255)
                                                                                                                           cATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTa                                                           <  2:1583532/118‑1 (MQ=255)
                                                                                                                                gTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCACTCGATgc                                                <  2:952045/124‑1 (MQ=255)
                                                                                                                                gTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCACTCGATGCCGCCAACCTGCTGGAAGTAGGTGAAc                      >  1:264073/1‑150 (MQ=255)
                                                                                                                                   aCCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCACTCGATGCCGCCAACCTGCTGGAAGTAGGTGAACTgg                   <  2:2424146/150‑1 (MQ=255)
                                                                                                                                     cTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGc                                                                                                     >  2:440846/1‑66 (MQ=255)
                                                                                                                                     cTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGc                                                                                                     <  1:440846/66‑1 (MQ=255)
                                                                                                                                     cTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGTGAGCACTCGATGCCGCCAACCTGCTGGAAGTAGGTGAACTGGGt                 >  1:2380694/1‑150 (MQ=255)
                                                                                                                                      ttGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCACTCGATGCCGCCAACCTGCTGGAAGTAGGTGAACTGGGTg                >  1:1778890/1‑150 (MQ=255)
                                                                                                                                       tGCCGAACGGGCCGTCGGCCCACACCAGGCCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGGAGCACTCGATGCCGCCAACCTGCTGGAAGTAGCTGAACTGGGtca               >  2:834861/1‑148 (MQ=255)
                                                                                                                                         tggtgtgggCCGACGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCACTCGATGCCGCCAACCTGCTGGAAGTAGGTGAACTGGGTGACt             >  1:1762853/7‑150 (MQ=255)
                                                                                                                                           gtgtgggCCGACGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCACTCGATgcc                                               <  1:488438/110‑1 (MQ=255)
                                                                                                                                           gtgtgggCCGACGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCACTCGATgcc                                               >  2:488438/5‑114 (MQ=255)
                                                                                                                                           gAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCACTCGATGCCGCCAACCTGCTGGAAGTAGGTGAACTGGTTGACTTc           <  2:2340041/150‑1 (MQ=255)
                                                                                                                                            aaCGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCACTCGATGCCGCCAACCTGCTGGAAGTAGGTGAACTGGGTGACTTcc          >  1:838055/1‑150 (MQ=255)
                                                                                                                                              tgggCCGACGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCACTCGATGCCGCCAACCTGCTGGAAGTAGGTGAACTGGGTGACTTCCAt        <  2:1762853/149‑1 (MQ=255)
                                                                                                                                               gggCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCACTCGATGCCGCCAACCTGCTGGAAGTAGGTGAACTGGGTGACTTCCATg       <  2:2245927/150‑1 (MQ=255)
                                                                                                                                                 gCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCACTCGATGCCGCCAACCTGCTGGAAGTAGGTGAACTgg                   <  1:2094160/136‑1 (MQ=255)
                                                                                                                                                   cGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCACTCGATGCCGCCAACCTGCTGGAAGTAGGTGAACTGGGTGACTTCCATGCCGt   <  2:722515/150‑1 (MQ=255)
                                                                                                                                                    gTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCACTCGATGCCGCCAACCTGCTGGAAGTAGGTGAACTGGGTGACTTCCATGCCGtt  <  2:908542/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCAGCTTGATCAGGCGGTTGGCTTCGCTTTCGTAGAAATCGAACAGTTCGAACAGTTTGTCGACGTTTGCATGTTCAAAGTTGTAGGTCGACTGCTCCACCTCGTTCTGGTGGAACACGTCGCCATAGGTGACCTTGCCGAACGGGCCGTCGGCCCACACCAGGTCGTACACCGAATCGACGCCCTGGATGTACATGGCCAGGCGCTCCAGGCCGTAGGTGATTTCACCGGTGACCGGGTAGCACTCGATGCCGCCAACCTGCTGGAAGTAGGTGAACTGGGTGACTTCCATGCCGTT  >  NC_002947/72134‑72431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑