Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	620,698	G→T	6.2%	intergenic (‑6/‑176)	PP_0534 ← / → PP_0535	transcriptional regulator PfeR/ferric siderophore receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	620,698	0	G	T	6.2%	200.8 / 2.1	64	intergenic (‑6/‑176)	PP_0534/PP_0535	transcriptional regulator PfeR/ferric siderophore receptor
Reads supporting (aligned to +/- strand):  ref base G (32/28);  new base T (2/2);  total (34/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.41e-01

GAACTGAGATTGCTGTATGGCATTCAGGGCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGTTAATCATGCTGCACCCTGGTTTTCGCTCCGGTATCGTTCGCAAAAAGCAACTTGTTTCGTTTGCGA  >  NC_002947/620552‑620844                                                                                                                                                   |                                                                                                                                                   gAACTGGGATTGCTGGATGGCATTCAGGGCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCt                                                                                                                                                 <  2:131928/150‑1 (MQ=255)    cTGAGATTGCTGTATGGCATTCAGGGCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTtga                                                                                                                                              <  1:613663/150‑1 (MQ=255)              tGTATGGCATTCAGGGCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAACCCGCGGTGGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAAtttt                                                                                                                                    >  2:2160577/1‑150 (MQ=255)              tGTATGGCATTCAGGGCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAAtttt                                                                                                                                    >  2:2032215/1‑150 (MQ=255)              tGTATGGCATTCAGGGCCTGGGTGCCGCTGGCGCAAGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAAtttt                                                                                                                                    >  1:1494607/1‑150 (MQ=255)                  tGGCATTCAGGGCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATc                                                                                                                                >  2:1834033/1‑150 (MQ=255)                       tgccgggCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGCTGCGGAAATGGCGGTCCAGGTCCTAAAGCAGGCGTGGGGCGTCTTCGACATGGAGACGAGAAAGCCGTTCGTCGAGCCTTGCTGAGAATTTTTATCAGTTg                                                                                                                           >  2:565426/5‑150 (MQ=255)                          aGGGCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGa                                                                                                                        >  1:1838526/1‑150 (MQ=255)                             gCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAatc                                                                                                                     >  2:926423/1‑150 (MQ=255)                              ccTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAatca                                                                                                                    >  1:2076753/1‑150 (MQ=255)                                tGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAatcatc                                                                                                                  <  1:2032215/150‑1 (MQ=255)                                       cGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTtgat                                                                                                                                             <  2:1411274/116‑1 (MQ=255)                                       cGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTtgat                                                                                                                                             >  1:1411274/1‑116 (MQ=255)                                        gCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCtt                                                                                                                                                <  1:478366/112‑1 (MQ=255)                                        gCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCtt                                                                                                                                                >  2:478366/1‑112 (MQ=255)                                        gCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCtt                                                                                                                                                <  1:1683461/112‑1 (MQ=255)                                        gCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCtt                                                                                                                                                >  2:1683461/1‑112 (MQ=255)                                            gcgcAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCa                                                                                                      <  2:214912/150‑1 (MQ=255)                                              gcAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGc                                                                                                                                                   <  1:1689598/103‑1 (MQ=255)                                              gcAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGc                                                                                                                                                   >  2:1689598/1‑103 (MQ=255)                                              gcAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAgg                                                                                                    >  1:1143521/1‑150 (MQ=255)                                              gcAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAgg                                                                                                    >  2:939159/1‑150 (MQ=255)                                                    gTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAgg                                                                                              <  2:1143521/150‑1 (MQ=255)                                                    gTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAgg                                                                                              <  2:2076753/150‑1 (MQ=255)                                                            ggAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAgg                                                                                              >  2:659175/1‑142 (MQ=255)                                                            ggAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAgg                                                                                              <  1:659175/142‑1 (MQ=255)                                                            ggAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTAGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGcc                                                                                      >  2:1231752/1‑150 (MQ=255)                                                                aCCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGa                                                                                  <  1:926423/150‑1 (MQ=255)                                                                     ggTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCa                                                                                                                               >  1:1617590/1‑100 (MQ=255)                                                                     ggTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCa                                                                                                                               <  2:1617590/100‑1 (MQ=255)                                                                       ttGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCt                                                                                                                                                 >  1:686634/1‑80 (MQ=255)                                                                       ttGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCt                                                                                                                                                 <  2:686634/80‑1 (MQ=255)                                                                            gAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTaagaag                                                                      >  2:2238642/1‑150 (MQ=255)                                                                             caaTGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAgg                                                                     >  2:962350/2‑150 (MQ=255)                                                                              aaTGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGt                                                                    <  1:939159/150‑1 (MQ=255)                                                                                     gTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGATCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGt                                                                    >  2:911320/1‑143 (MQ=255)                                                                                     gTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGATCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGt                                                                    <  1:911320/143‑1 (MQ=255)                                                                                     gTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGATCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGt                                                                    >  2:2146298/1‑143 (MQ=255)                                                                                     gTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGATCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGt                                                                    <  1:2146298/143‑1 (MQ=255)                                                                                      tCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAACGGCAACGCCTTGATGTGTTAAGAAGGTTAATCAtg                                                            <  2:1279126/150‑1 (MQ=255)                                                                                       ccAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGTTAATCAtgc                                                           >  1:658773/1‑150 (MQ=255)                                                                                        cAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGTTAATCAtgct                                                          >  1:915856/1‑150 (MQ=255)                                                                                         aGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGt                                                                    <  2:901203/139‑1 (MQ=255)                                                                                         aGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGt                                                                    >  1:901203/1‑139 (MQ=255)                                                                                           gTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGTTAATCATGCTGCa                                                       >  1:599066/1‑150 (MQ=255)                                                                                             ccTGAAGCAGGCGTGGGTCGTCTTCGGCATGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGTTAATCATGCTGCAcc                                                     >  2:765642/1‑150 (MQ=255)                                                                                              cTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGa                                                                                  <  1:1810698/120‑1 (MQ=255)                                                                                              cTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGa                                                                                  >  2:1810698/1‑120 (MQ=255)                                                                                                gAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGTTAATCATGCTGCACCCTg                                                  <  2:653633/150‑1 (MQ=255)                                                                                                gAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGTTAATCATGCTGCACCCTg                                                  <  2:658773/150‑1 (MQ=255)                                                                                                   gCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGTTAATCATGCTGCACCCTGGtt                                               <  1:1231752/150‑1 (MQ=255)                                                                                                     aGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGTTAATCATGCTGCACCCTGGtttt                                             >  1:1178456/1‑150 (MQ=255)                                                                                                      ggCGTGGGTCGTCTTCGGCAAGGAGCAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGTTAATCATGCAGCACCCTATTTTTc                                            <  2:222239/150‑1 (MQ=255)                                                                                                       gCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCATGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGTTAATCATGCTGCACCCTGGTTTTCg                                           >  2:1229431/1‑150 (MQ=255)                                                                                                             gtcgtcTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGTTAATCATGCTGCACCCTGGTTTTCGCTCCgg                                     >  2:1877623/1‑150 (MQ=255)                                                                                                                 tcTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAggg                                                                                             <  1:2154353/90‑1 (MQ=255)                                                                                                                 tcTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAggg                                                                                             >  2:2154353/1‑90 (MQ=255)                                                                                                                        cAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGTTAATCATGCTGCACCCTGGTTTTCGCTCCGGTATCGTTCGCa                          >  2:883470/1‑150 (MQ=255)                                                                                                                                aGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGTTAATCATGCTGCACCCTGGTTTTCGCTCCGGTATCGTTCGCAAAAAGCaa                  <  1:765642/150‑1 (MQ=255)                                                                                                                                aGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGTTAATCATGCTGCACCCTGGTTTTCGCTCCGGTATCGTTCGCAAAAAGCaa                  <  1:1877623/150‑1 (MQ=255)                                                                                                                                      gTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGTTAATCATGCTGCACCCTGGTTTTCGCTCCGGTATCGTTCGCAAAAAGCAACTTGtt            >  2:15274/1‑150 (MQ=255)                                                                                                                                           cACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGTTAATCATGCTGCACCCTGGTTTTCGCTCCGGTATCGTTCGCAAAAAGCAACTTGTTTCGtt       <  2:1178456/150‑1 (MQ=255)                                                                                                                                              ggCGCGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGTTAATCATGCTGCACCCTGGTTTTCGCTCCGGTATCGTTCGCAAAAAGCAACTTGTTTCGTTTGc    <  2:1494607/150‑1 (MQ=255)                                                                                                                                                cGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGTTAATCATGCTGCACCCTGGTTTTCGCTCCGGTATCGTTCGCAAAAAGCAACTTGTTTCGTTTGCGa  <  2:915856/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   GAACTGAGATTGCTGTATGGCATTCAGGGCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGTTAATCATGCTGCACCCTGGTTTTCGCTCCGGTATCGTTCGCAAAAAGCAACTTGTTTCGTTTGCGA  >  NC_002947/620552‑620844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑