Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 86,240 G→T 6.4% L160M (CTG→ATG)  PP_0075 ← choline sulfate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294786,2400GT 6.4% 391.4 / 10.8 125L160M (CTG→ATG) PP_0075choline sulfate transporter
Reads supporting (aligned to +/- strand):  ref base G (59/58);  new base T (4/4);  total (63/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.74e-01

Only 100 of 132 total aligned reads displayed.

CAACAGGCTCGGCCAGCGTGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGAAAATGCCGCCGA  >  NC_002947/86091‑86386
                                                                                                                                                     |                                                                                                                                                  
cAACAGGCTCGGCCAGCGTGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAg                                                                                                                                                    <  2:586965/150‑1 (MQ=255)
cAACAGGCACGGCCAGCGTGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAg                                                                                                                                                    <  2:1316397/150‑1 (MQ=255)
  aCAGGCTCGGCCAGCGTGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAggg                                                                                                                                                  <  1:2272033/150‑1 (MQ=255)
  aCAGGCTCGGCCAGCGTGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAggg                                                                                                                                                  >  2:1127525/1‑150 (MQ=255)
  aCAGGCTCGGCCAGCGTGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAggg                                                                                                                                                  <  1:54800/150‑1 (MQ=255)
      gCTCGGCCAGCGTGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCAt                                                                                                                                              <  2:2336845/150‑1 (MQ=255)
        tCGGCCAGCGTGGGCGCCTGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGc                                                                                                                                            >  1:1380571/1‑150 (MQ=255)
        tCGGCCAGCGTGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGc                                                                                                                                            >  2:393069/1‑150 (MQ=255)
        tCGGCCAGCGTGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGc                                                                                                                                            >  2:881294/1‑150 (MQ=255)
            ccAGCGTGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCcag                                                                                                                                        >  2:660124/1‑150 (MQ=255)
             cAGCGTGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCATGGCCCTGCCcagc                                                                                                                                       <  1:427362/150‑1 (MQ=255)
              aGCGTGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCcagca                                                                                                                                      <  2:709712/150‑1 (MQ=255)
               gCGTGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCcagcag                                                                                                                                     <  1:214652/150‑1 (MQ=255)
                cGTGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCATGGCCCTGCCcagccag                                                                                                                                    <  2:984516/150‑4 (MQ=255)
                  tGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGTAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGtt                                                                                                                                  >  2:537477/1‑150 (MQ=255)
                     gcgcCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTggg                                                                                                                               <  1:2288484/150‑1 (MQ=255)
                     gcgcCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTggg                                                                                                                               <  1:2307772/150‑1 (MQ=255)
                       gcCAGAGTGTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTa                                                                                                                             >  2:594905/1‑150 (MQ=255)
                         cAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGc                                                                                                                           >  2:2115560/1‑150 (MQ=255)
                         cAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGc                                                                                                                           >  2:1907360/1‑150 (MQ=255)
                         cAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGc                                                                                                                           >  2:1492043/1‑150 (MQ=255)
                         cAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGACGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGc                                                                                                                           >  1:317742/1‑150 (MQ=255)
                            agTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCGCAGCAGGTTGGGTAGCTgg                                                                                                                        <  1:2117839/150‑1 (MQ=255)
                                 gAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCaa                                                                                                                   <  1:1381682/150‑1 (MQ=255)
                                     gCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCcag                                                                                                                                        >  2:1540476/1‑125 (MQ=255)
                                         cACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGa                                                                                                           >  2:1766232/1‑150 (MQ=255)
                                         cACCGATCACGACGGTGGCCAGGACCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGATGGCCAGGTGCTGCACGGTCATCAGCGCGGTGGCGTGGATGGGCAGGGGCATGCCCAGCAGGTTGGGGAGCTGGCCCAAGGCAATGa                                                                                                           >  1:862723/1‑150 (MQ=255)
                                          aCCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGAc                                                                                                          <  1:1492043/150‑1 (MQ=255)
                                            cGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGa                                                                                                        >  2:2310811/1‑150 (MQ=255)
                                            cGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGa                                                                                                        <  2:337248/150‑1 (MQ=255)
                                            cGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGa                                                                                                        >  2:974940/1‑150 (MQ=255)
                                               tCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGTTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATgg                                                                                                     <  1:974940/150‑1 (MQ=255)
                                               tCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATgg                                                                                                     >  1:1934341/1‑150 (MQ=255)
                                                 acgacgGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATggcg                                                                                                   <  1:1127525/150‑1 (MQ=255)
                                                 acgacgGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATggcg                                                                                                   <  2:1993465/150‑1 (MQ=255)
                                                  cgacgGTGGCCAGGCCCAGCAGCAAGGAGGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATggcgg                                                                                                  >  2:1200001/1‑150 (MQ=255)
                                                   gacgGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATggcggc                                                                                                 >  2:2256786/1‑150 (MQ=255)
                                                    acgGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCa                                                                                                >  2:1710453/1‑150 (MQ=255)
                                                         ggCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTgg                                                                                                                                <  2:32711/113‑1 (MQ=255)
                                                         ggCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTgg                                                                                                                                >  1:32711/1‑113 (MQ=255)
                                                               gCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGaa                                                                                     >  1:1584770/1‑150 (MQ=255)
                                                                cccAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAg                                                                                    <  1:2310811/150‑1 (MQ=255)
                                                                 ccAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGc                                                                                   >  1:2110701/1‑150 (MQ=255)
                                                                    gcagcaAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGa                                                                                >  1:670115/1‑150 (MQ=255)
                                                                           ggaaggTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGc                                                                                   <  1:2020114/140‑1 (MQ=255)
                                                                            gaaggTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACg                                                                        >  1:133300/1‑150 (MQ=255)
                                                                                gtaggtCGACCTCGCCTGCGTGGCTGGCCAGTTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGTCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAAt                                                                    <  2:862723/150‑1 (MQ=255)
                                                                                gtaggtCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCcagcag                                                                                                                                     >  2:213622/1‑85 (MQ=255)
                                                                                gtaggtCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCcagcag                                                                                                                                     <  1:213622/85‑1 (MQ=255)
                                                                                gtaggtCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCGGCGTGAAGCCGAGCACCACGGAAt                                                                    <  1:660124/150‑1 (MQ=255)
                                                                                 taggtCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATg                                                                   <  1:2119366/150‑1 (MQ=255)
                                                                                  atctCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACCAACATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATgg                                                                  <  1:1205047/147‑1 (MQ=255)
                                                                                  aggtCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATgg                                                                  <  2:428097/150‑1 (MQ=255)
                                                                                  aggtCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATgg                                                                  <  1:1200001/150‑1 (MQ=255)
                                                                                   ggtCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATggg                                                                 <  1:2021924/150‑1 (MQ=255)
                                                                                    gtCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCATGGCCCTGCCcagcca                                                                                                                                     >  2:153082/1‑79 (MQ=38)
                                                                                    gtCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCATGGCCCTGCCcagcca                                                                                                                                     <  1:153082/81‑3 (MQ=38)
                                                                                    gtCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGga                                                                >  2:283454/1‑150 (MQ=255)
                                                                                    gtCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGga                                                                <  1:910767/150‑1 (MQ=255)
                                                                                    gtCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATAAGCGCGGTGGCCTGGCTGGGCAGGTCCATGCCAAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGga                                                                <  1:1742592/150‑1 (MQ=255)
                                                                                      cGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGgaga                                                              <  2:1934341/150‑1 (MQ=255)
                                                                                       gACCTCGCCTGCGTGGCTGGCCAGGTTCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCACGACGACAACCATAGCGGCACCCAGCCGTAATCCGAGCACCACGGAATGGgggat                                                             >  2:1205047/1‑149 (MQ=255)
                                                                                          cTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGaa                                                          >  1:1275604/1‑150 (MQ=255)
                                                                                          cTCGCCTGCGTGGCTGGCCAGGTCCTGAACGGAAATCAGCGCGGTGTCCTGGCGGGGCAGGGCCATGATCAGGAGGTTGGCTAGCCCGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAAGGGGAGACGaa                                                          >  1:1994231/1‑150 (MQ=255)
                                                                                           tCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGCGTACCACGGAATGGGAGACGAAg                                                         <  2:475844/150‑1 (MQ=255)
                                                                                             gCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTg                                                                                       <  2:2392847/118‑1 (MQ=255)
                                                                                             gCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTg                                                                                       >  1:2392847/1‑118 (MQ=255)
                                                                                             gCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGtt                                                       <  2:264992/150‑1 (MQ=255)
                                                                                               cTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGa                                                     >  2:612992/1‑150 (MQ=255)
                                                                                               cTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCCGGGCAGGGCCAAGCACAGCAGGTTGGGTAGCTGGACCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAACGGGAGACGAAGTTGa                                                     >  2:926105/1‑150 (MQ=255)
                                                                                                tGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCCACCGCAGGAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGAc                                                    <  2:1380571/150‑1 (MQ=255)
                                                                                                   gTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAg                                                 <  1:283454/150‑1 (MQ=255)
                                                                                                    tggctggcCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAgc                                                <  1:881294/150‑1 (MQ=255)
                                                                                                     ggctggcCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAgcg                                               >  1:1490595/1‑150 (MQ=255)
                                                                                                         ggcCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGcc                                           >  2:209982/1‑150 (MQ=255)
                                                                                                          gcCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGtt                                                                                                                                  <  2:1758033/62‑1 (MQ=255)
                                                                                                          gcCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCg                                          >  2:1179565/1‑150 (MQ=255)
                                                                                                           ccAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGa                                         >  1:1828907/1‑150 (MQ=255)
                                                                                                            cAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGaa                                        >  2:346472/1‑150 (MQ=255)
                                                                                                            cAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGaa                                        <  2:133300/150‑1 (MQ=255)
                                                                                                            cAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGaa                                        <  2:569448/150‑1 (MQ=255)
                                                                                                            cAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGaa                                        <  1:594905/150‑1 (MQ=255)
                                                                                                            cAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGaa                                        >  1:685284/1‑150 (MQ=255)
                                                                                                                tCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGAc                                                    <  1:2283595/134‑1 (MQ=255)
                                                                                                                    gCACGGTCATGAGCGGGGTGGGCTGGCTGGGCAGGGCCATGCCCCGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCCGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATa                                <  2:317742/150‑1 (MQ=255)
                                                                                                                       cGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGcc                             <  2:685284/150‑1 (MQ=255)
                                                                                                                       cGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGcc                             <  1:1544021/150‑1 (MQ=255)
                                                                                                                        ggTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCTCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGccc                            <  1:393069/150‑1 (MQ=255)
                                                                                                                          tcatcaGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATa                                >  1:743306/1‑144 (MQ=255)
                                                                                                                          tcatcaGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATa                                <  2:743306/144‑1 (MQ=255)
                                                                                                                             tcaGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGagca                       <  2:534116/150‑1 (MQ=255)
                                                                                                                                      tGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGa              >  1:284571/1‑150 (MQ=255)
                                                                                                                                        aacctgctggGCATGGCCCTGCCCAGCCGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGaaa            >  1:2263259/6‑150 (MQ=255)
                                                                                                                                         ccTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGaaaa           <  2:284571/150‑1 (MQ=255)
                                                                                                                                          ctggctggGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGAAAAt          <  2:2110701/150‑1 (MQ=255)
                                                                                                                                           ctgctggGCATGGCCCTGCCCAGCCGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGAAAATg         >  1:194648/3‑150 (MQ=255)
                                                                                                                                               tggGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATgg                                                                  >  2:2157209/1‑89 (MQ=255)
                                                                                                                                               tggGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATgg                                                                  <  1:2157209/89‑1 (MQ=255)
                                                                                                                                               tggGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGAAATAGCCCGAGCAGCAGCTGGAAAATgccgc     >  2:1246586/1‑150 (MQ=255)
                                                                                                                                                  gCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGAAAATGCCGCCGa  <  1:622749/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CAACAGGCTCGGCCAGCGTGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGAAAATGCCGCCGA  >  NC_002947/86091‑86386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑