Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 98,690 A→T 6.1% Q39L (CAG→CTG)  PP_0093 → zinc protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294798,6900AT 6.1% 307.1 / 8.9 98Q39L (CAG→CTG) PP_0093zinc protease
Reads supporting (aligned to +/- strand):  ref base A (48/44);  new base T (4/2);  total (52/46)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01

TCAGACAGTCCCGGATGCTTCGACTTTTTCTTATGCAGCGCTCAGGCCTTGGGGCACTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCGGGTGTTTGCCAGCCAGCAATTGAAACTGCCAGACAACCGCA  >  NC_002947/98543‑98839
                                                                                                                                                   |                                                                                                                                                      
tCAGACAGTCCCGGATGCTTCGACTTTTTCTTATGCAGCGCTCAGGCCTTGGGGCACTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATTTCGCCTACTATGGGCAGCTGGCCCAgg                                                                                                                                                      <  2:1037599/150‑1 (MQ=255)
tCAGACAGTCCCGGATGCTTCGACTTTTTCTTATGCAGCGCTCAGGCCTTGGGGCACTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAgg                                                                                                                                                      >  2:2106612/1‑150 (MQ=255)
 cAGACAGTCCCGGATGCTTCGACTTTTTCTTATGCAGCGCTCAGGCCTTGGGGCACTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAggg                                                                                                                                                     <  2:303164/150‑1 (MQ=255)
   gACAGTCCCGGATGCTTCGACTTTTTCTTATGCAGCGCTCAGGCCTTGGGGCACTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGcc                                                                                                                                                   >  1:696041/1‑150 (MQ=255)
    aCAGTCCCGGATGCTTCGACTTTTTCTTATGCAGCGCTCAGGCCTTGGGGCACTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCa                                                                                                                                                  <  2:2257854/150‑1 (MQ=255)
    aCAGTCCCGGATGCTTCGACTTTTTCTTATGCAGCGCTCAGGCCTTGGGGCACTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCa                                                                                                                                                  <  2:1228150/150‑1 (MQ=255)
     cAGTCCCGGATGCTTCGACTTTTTCTTATGCAGCGCTCAGGCCTTGGGGCACTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAg                                                                                                                                                 <  1:1443266/150‑1 (MQ=255)
        tCCCGGATGCTTCGACTTTTTCTTATGCAGCGCTCAGGCCTTGGGGCACTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTgg                                                                                                                                              <  1:1372259/150‑1 (MQ=255)
            ggATTCTGCTACTTTTTCTTATGCAGCGCTCAGGCCTGGGGGCACTCTACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGCCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGt                                                                                                                                          <  1:1009451/150‑1 (MQ=255)
             gATGCTTCGACTTTTTCTTATGCAGCGCTCAGGCCTTGGGGCACTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGtt                                                                                                                                         <  2:579602/150‑1 (MQ=255)
             gATGCTTCGACTTTTTCTTATGCAGCGCTCAGGCCTTGGGGCACTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGtt                                                                                                                                         >  1:579602/1‑150 (MQ=255)
             gATGCTTCGACTTTTTCTTATGCAGCGCTCAGGCCTTGGGGCACTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGtt                                                                                                                                         <  1:2392537/150‑1 (MQ=255)
                 cTTCGACTTTTTCTTATGCAGCGCTCAGGCCTTGGGGCACTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTtgctg                                                                                                                                     >  1:240517/1‑150 (MQ=255)
                    cGACTTTTTCTTATGCAGCGCTCAGGCCTTGGGGCACTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCCATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCgg                                                                                                                                  <  2:743656/150‑1 (MQ=255)
                    cGACTTTTTCTTATGCAGCGCTCAGGCCTTGGGGCACTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCgg                                                                                                                                  <  2:696041/150‑1 (MQ=255)
                        tttttCTTATGCAGCGCTCAGGCCTTGGGGCACTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAgg                                                                                                                                                      >  2:928253/1‑126 (MQ=255)
                        tttttCTTATGCAGCGCTCAGGCCTTGGGGCACTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAgg                                                                                                                                                      <  1:928253/126‑1 (MQ=255)
                        tttttCTTATGCAGCGCTCAGGCCTTGGGGCACTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGgcgc                                                                                                                              <  2:986167/150‑1 (MQ=255)
                            tCTTATGCAGCGCTCAGGCCTTGGGGCACTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCa                                                                                                                          >  2:368544/1‑150 (MQ=255)
                            tCTTATGCAGCGCTCAGGCCTTGGGGCACTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCCGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCa                                                                                                                          <  2:2278610/150‑1 (MQ=255)
                               tATGCAGCGCTCAGGCCTTGGGGCACTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTAGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGcc                                                                                                                       >  2:465184/1‑150 (MQ=255)
                                aTGCAGCGCTCAGGCCTTGGGGCACTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCt                                                                                                                      >  2:307375/1‑150 (MQ=255)
                                   cAGCGCTCAGGCCTTGGGGCACTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCTGCAGccgtta                                                                                                                   <  2:1971177/150‑5 (MQ=255)
                                    aGCGCTCAGGCCTTGGGGCACTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTag                                                                                                                  >  2:1230769/1‑150 (MQ=255)
                                     gcgcTCAGGCCTTGGGGCACTCGACCGCATTTTCACCCGTTTGGTTACGTTGCCTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTTGGCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTaga                                                                                                                 >  1:1879901/1‑150 (MQ=255)
                                        cTCAGGCCTTGGGGCACTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCa                                                                                                              <  1:51363/150‑1 (MQ=255)
                                         tCAGGCCTTGGGGCACTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAg                                                                                                             >  2:266882/1‑150 (MQ=255)
                                               cTTGGGGCACTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTg                                                                                                       <  1:556200/150‑1 (MQ=255)
                                                ttGGGGCACTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCGGGTATTgg                                                                                                      >  2:88762/1‑150 (MQ=255)
                                                 tGGGGCACTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGc                                                                                                     <  2:1803901/150‑1 (MQ=255)
                                                  ggggCACTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGcc                                                                                                    <  1:2106612/150‑1 (MQ=255)
                                                   gggCACTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCg                                                                                                   >  1:2268813/1‑150 (MQ=255)
                                                      cACTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGAcc                                                                                                <  2:555352/150‑1 (MQ=255)
                                                       aCTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCTGGGCCAgc                                                                                                                                                <  2:949902/101‑2 (MQ=255)
                                                       aCTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCTGGGCCAgc                                                                                                                                                >  1:949902/1‑100 (MQ=255)
                                                           gACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑t                                                                                          >  2:2045658/1‑150 (MQ=255)
                                                              cGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TAcc                                                                                       >  2:1066842/1‑150 (MQ=255)
                                                              cGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TAcc                                                                                       >  1:988460/1‑150 (MQ=255)
                                                               gCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TAccc                                                                                      <  1:1230769/150‑1 (MQ=255)
                                                               gCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TAccc                                                                                      >  1:2294564/1‑150 (MQ=255)
                                                                  ttttCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTg                                                                                   <  2:2271917/150‑1 (MQ=255)
                                                                      cACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAg                                                                                                             <  2:1834271/121‑1 (MQ=255)
                                                                      cACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAg                                                                                                             >  1:1834271/1‑121 (MQ=255)
                                                                       aCCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCAccc                                                                              <  1:1066842/150‑1 (MQ=255)
                                                                       aCCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCAccc                                                                              >  1:1431433/1‑150 (MQ=255)
                                                                       aCCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCAccc                                                                              >  2:1005717/1‑150 (MQ=255)
                                                                       aCCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCAccc                                                                              >  1:1499900/1‑150 (MQ=255)
                                                                        cccGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑Ta                                                                                         <  1:754059/138‑1 (MQ=255)
                                                                        cccGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑Ta                                                                                         >  2:754059/1‑138 (MQ=255)
                                                                         ccGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCtt                                                                            >  2:422504/1‑150 (MQ=255)
                                                                         ccGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCtt                                                                            >  2:1621830/1‑150 (MQ=255)
                                                                                 ttCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTgcgcgcgc                                                                    >  1:2198192/1‑150 (MQ=255)
                                                                                 ttCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTgcgcgcgc                                                                    >  1:2193864/1‑150 (MQ=255)
                                                                                 ttCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTgcgcgcgc                                                                    >  1:372969/1‑150 (MQ=255)
                                                                                  tCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCa                                                                   >  1:1611603/1‑150 (MQ=255)
                                                                                   ccGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGAcc                                                                                                >  1:418336/1‑121 (MQ=255)
                                                                                   ccGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGAcc                                                                                                <  2:418336/121‑1 (MQ=255)
                                                                                    cGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCAcc                                                                 >  1:1602027/1‑150 (MQ=255)
                                                                                             cggcgCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAgg                                                        <  1:2045658/150‑1 (MQ=255)
                                                                                              ggcgCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGc                                                       <  2:2268813/150‑1 (MQ=255)
                                                                                                 gcTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCACAAGGCCGa                                                    >  1:1076413/1‑150 (MQ=255)
                                                                                                   tCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAAc                                                  >  1:137365/1‑150 (MQ=255)
                                                                                                      tgctgAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACCGGCCGAACAgg                                               >  2:753085/1‑150 (MQ=255)
                                                                                                      tgctgAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAgg                                               >  2:2285004/1‑150 (MQ=255)
                                                                                                           aaCGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCgg                                          >  2:1069200/1‑150 (MQ=255)
                                                                                                           aaCGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGACAGTTGCTGCGGGCGCGGCAGCCTGTCGCGAAGGCCAT‑GCGGAATACGCGGATGTGTTCGCCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCgg                                          <  1:646022/150‑1 (MQ=255)
                                                                                                            aCGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCggg                                         <  1:307375/150‑1 (MQ=255)
                                                                                                                ttGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCGGGTGtt                                     <  1:1137516/150‑1 (MQ=255)
                                                                                                                 tGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCGGGTGttt                                    <  1:266882/150‑1 (MQ=255)
                                                                                                                  gcagcaATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCGGGTGttt                                    >  1:1911801/1‑149 (MQ=255)
                                                                                                                  gcagcaATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCGGGGGttt                                    <  2:1911801/149‑1 (MQ=255)
                                                                                                                   cagcaATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCGGGTGTTTgc                                  >  1:2432322/1‑150 (MQ=255)
                                                                                                                   cagcaATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTACACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCGGGTGTTTgc                                  >  1:1611769/1‑150 (MQ=255)
                                                                                                                    agcaATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCGGGTGTTTgcc                                 >  2:12900/1‑150 (MQ=255)
                                                                                                                    agcaATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCGGGTGTTTgcc                                 >  2:1634766/1‑150 (MQ=255)
                                                                                                                     gcaATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCGGGTGTTTgcca                                >  1:2185600/1‑150 (MQ=255)
                                                                                                                     gcaATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCGGGTGTTTgcca                                <  2:2185600/150‑1 (MQ=255)
                                                                                                                             gCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCGGGTGTTTGCCAGCCAGCaa                        <  2:2294564/150‑1 (MQ=255)
                                                                                                                               ctactaTGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCGGGTGTTTGCCAGCCAGCAAtt                      <  2:372969/150‑1 (MQ=255)
                                                                                                                                 actcTGGGCAGCTGGCCCAGTGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCGGGTGTTTGCCAGCCAGCAATTGa                    <  1:355678/150‑1 (MQ=255)
                                                                                                                                  ctaTGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCGGGTGTTTgccagc                              <  2:2188752/139‑1 (MQ=255)
                                                                                                                                  ctaTGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCGGGTGTTTgccagc                              >  1:2188752/1‑139 (MQ=255)
                                                                                                                                   taTGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCGGGTGTTTGCCAGCCAGCAATTGaaa                  >  2:1443357/1‑150 (MQ=255)
                                                                                                                                      gggCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCGGGTGTTTGccagccag                           <  1:1650414/138‑1 (MQ=255)
                                                                                                                                      gggCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCGGGTGTTTGccagccag                           >  2:1650414/1‑138 (MQ=255)
                                                                                                                                       tgccacTGGCCCTGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCGGGTGttt                                    >  2:2213506/6‑128 (MQ=255)
                                                                                                                                       tgccacTGGCCCTGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCGGGTGttt                                    <  1:2213506/123‑1 (MQ=255)
                                                                                                                                        gCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCGGGTGTTTGCCAGCCAGCAATTGAAACTGcc             <  1:1005717/150‑1 (MQ=255)
                                                                                                                                         ccacTGGCCCTGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCGGGTGTTTTCCAGCCAGCAATTGAAACTGCCa            >  2:1485056/4‑150 (MQ=255)
                                                                                                                                          cacTGGCCCTGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCGGGTGTTTGCCAGCCAGCAATTGAAACTGCCAg           >  2:2014295/3‑150 (MQ=255)
                                                                                                                                          aGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGgcgc                                            <  2:740957/117‑1 (MQ=255)
                                                                                                                                          aGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGgcgc                                            >  1:740957/1‑117 (MQ=255)
                                                                                                                                          aGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCGGGTGTTTGCCAGCCAGCAAt                       <  2:1578753/138‑1 (MQ=255)
                                                                                                                                          aGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCGGGTGTTTGCCAGCCAGCAAt                       >  1:1578753/1‑138 (MQ=255)
                                                                                                                                           gCTGGCCCAGGGCACCTGTCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCGGGTGTTTGCCAGCCAGCAATTGAAACTGCCAGa          >  1:81904/1‑150 (MQ=255)
                                                                                                                                                cccAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑AAATCGTGTACCTGTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCGGGTGTTTGCCAGCCAGCAATTGAAACTGCCAGACAAcc     <  2:81904/150‑1 (MQ=255)
                                                                                                                                                  cAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCGGGTGTTTGCCAGCCAGCAATTGAAACTGCCAGACAACCGc   <  1:422504/150‑1 (MQ=255)
                                                                                                                                                  cAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCGGGTGTTTGCCAGCCAGCAATTGAAACTGCCAGACAACCGc   >  2:2255835/1‑150 (MQ=255)
                                                                                                                                                   aGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCGGGTGTTTGCCAGCCAGCAATTGAAACTGCCAGACAACCGCa  <  1:368544/150‑1 (MQ=255)
                                                                                                                                                   aGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCGGGTGTTTGCCAGCCAGCAATTGAAACTGCCAGACAACCGCa  <  2:240517/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
TCAGACAGTCCCGGATGCTTCGACTTTTTCTTATGCAGCGCTCAGGCCTTGGGGCACTCGACCGCATTTTCACCCGTTTGGTTCCGCTGTTTGCGGCGCTCCTGCTGAACGGTTGCAGCAATGTCGCCTACTATGGGCAGCTGGCCCAGGGCCAGTGGCAGTTGCTGCGGGCGCGGCAGCCTGTAGAGCAGGTATTGGCCGACCCTGC‑TACCCCTGCACCCTTGCGCGCGCACCTGCAACAGGCCGAACAGGCGCGGGTGTTTGCCAGCCAGCAATTGAAACTGCCAGACAACCGCA  >  NC_002947/98543‑98839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑