BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	931,510	A→C	6.9%	D4705E (GAT→GAG)	PP_0806 ←	surface adhesion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	931,510	0	A	C	6.9%	315.6 / 11.3	111	D4705E (GAT→GAG)	PP_0806	surface adhesion protein
Reads supporting (aligned to +/- strand): ref base A (49/53); new base C (4/4); total (53/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

AGCAGCTCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTGTTGGCCGTTGGCCTGAGGCGATACGAGGTTGACCTG  >  NC_002947/931364‑931659

agcagcTCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGATGGTGTCACCCTCa                                                                                                                                                    <  1:1165919/150‑1 (MQ=255)

agcagcTCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCa                                                                                                                                                    >  1:906288/1‑150 (MQ=255)

  cagcTCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCAcc                                                                                                                                                  >  2:1734734/1‑150 (MQ=255)

   agcTCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCg                                                                                                                                                 <  2:2408299/150‑1 (MQ=255)

   agcTCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGAAGGTAGAGCTGCCGCTTTCCTCGCCTGCCGTGTTGGTCACTGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCg                                                                                                                                                 <  2:866045/150‑1 (MQ=255)

     cTCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGa                                                                                                                                               >  1:2108371/1‑150 (MQ=255)

      tCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGaa                                                                                                                                              >  1:1969329/1‑150 (MQ=255)

      tCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGCCGCTGCCGAGTTCCTCGCCTGCCGGGTTGGTCACGTCAACCCGTCCGCCCACTTCGCCGCGGCCGGCGAGGCTCACACCACCACCggca                                                                                                                                              >  2:316087/1‑148 (MQ=255)

       cGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGaaa                                                                                                                                             <  2:1557262/150‑1 (MQ=255)

          cGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACgg                                                                                                                                          >  2:1552696/1‑150 (MQ=255)

           gTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGc                                                                                                                                         >  1:1917776/1‑150 (MQ=255)

           gTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGCGACACCATCACCGGAAACGGc                                                                                                                                         <  1:266903/150‑1 (MQ=255)

              gCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAg                                                                                                                                      >  1:2045089/1‑150 (MQ=255)

                ccAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGtt                                                                                                                                    >  1:464195/1‑150 (MQ=255)

                ccAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGtt                                                                                                                                    >  2:292842/1‑150 (MQ=255)

                   gTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGc                                                                                                                                 <  2:2383692/150‑1 (MQ=255)

                    tGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGcc                                                                                                                                <  2:464195/150‑1 (MQ=255)

                        gggCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGt                                                                                                                            >  1:1846133/1‑150 (MQ=255)

                        gggCGCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGGCGTGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGt                                                                                                                            <  1:404864/150‑1 (MQ=255)

                            tCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCg                                                                                                                        >  2:1602683/1‑150 (MQ=255)

                             cAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGa                                                                                                                       <  1:1898174/150‑1 (MQ=255)

                               gCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATg                                                                                                                     >  1:2148135/1‑150 (MQ=255)

                                cGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGGGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATgg                                                                                                                    <  2:1596557/150‑1 (MQ=255)

                                      ggTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGgtgt                                                                                                              <  1:1009536/150‑1 (MQ=255)

                                      ggTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGgtgt                                                                                                              <  1:2051842/150‑1 (MQ=255)

                                      ggTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTCCTGGATGCACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGgtgt                                                                                                              >  1:1331244/1‑150 (MQ=255)

                                          acgTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCTGGTGCCGATGGCGgtgtcaac                                                                                                          <  1:1902646/148‑5 (MQ=255)

                                              ggCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCgg                                                                                                      <  1:502941/150‑1 (MQ=255)

                                              ggCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCgg                                                                                                      <  2:2108371/150‑1 (MQ=255)

                                               gCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTTGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCggg                                                                                                     <  1:2076158/150‑1 (MQ=255)

                                               gCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCgg                                                                                                      <  2:2118842/149‑1 (MQ=255)

                                               gCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCgg                                                                                                      >  1:2118842/1‑149 (MQ=255)

                                                    tGGCAGCGACGGTGGCGTTGATGATTTCCTAGCCTGCCCGCTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCgg                                                                                                <  2:624664/150‑1 (MQ=255)

                                                      gCATTGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACg                                                                                                                                           <  1:1142928/105‑1 (MQ=255)

                                                      gCATTGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACg                                                                                                                                           >  2:1142928/1‑105 (MQ=255)

                                                      gCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTg                                                                                              >  1:465712/1‑150 (MQ=255)

                                                       cATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGa                                                                                             <  2:2373529/150‑1 (MQ=255)

                                                        aTCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGAt                                                                                            >  1:1784282/1‑150 (MQ=255)

                                                         tCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGGCAGTTCGCCGGGTGCCGATGGCGGTGTGGTAGCGGGCGCGGTGATa                                                                                           >  1:1696976/1‑150 (MQ=255)

                                                         tCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATa                                                                                           <  2:850732/150‑1 (MQ=255)

                                                            aCGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAgcg                                                                                        >  2:275083/1‑150 (MQ=255)

                                                                   cgcTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTggcgg                                                                                 <  2:2047938/150‑1 (MQ=255)

                                                                    gcTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCg                                                                                                                  <  2:968859/116‑1 (MQ=255)

                                                                    gcTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCg                                                                                                                  >  1:968859/1‑116 (MQ=255)

                                                                        ccGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGa                                                                                             >  2:2046660/1‑133 (MQ=255)

                                                                        ccGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGa                                                                                             <  1:2046660/133‑1 (MQ=255)

                                                                          gATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTg                                                                          >  2:2014147/1‑150 (MQ=255)

                                                                          gATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTg                                                                          <  2:2148135/150‑1 (MQ=255)

                                                                            tttCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGt                                                                        <  1:2014147/150‑1 (MQ=255)

                                                                             ttCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGtt                                                                       <  2:465712/150‑1 (MQ=255)

                                                                                cTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTtgcc                                                                    <  2:1784282/150‑1 (MQ=255)

                                                                                cTCGCCTGCCGGGTTGGTCACGGTAACCCGGGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGAATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTtgcc                                                                    >  2:1411370/1‑150 (MQ=255)

                                                                                 tCGCCTGCCGGGTTGGTCACGGTAACCCTTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTtgcct                                                                   >  1:184304/1‑150 (MQ=255)

                                                                                 tCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTTGTATCGGGTGCGGTGATAGCGCTGGCGGCAGATTGGTtgcct                                                                   <  1:292842/150‑1 (MQ=255)

                                                                                 tCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTa                                                                                                          >  2:2158876/1‑111 (MQ=255)

                                                                                 tCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTa                                                                                                          <  1:2158876/111‑1 (MQ=255)

                                                                                 tCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCt                                                                                      >  2:800756/1‑131 (MQ=255)

                                                                                 tCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCt                                                                                      <  1:800756/131‑1 (MQ=255)

                                                                                 tCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTtgcct                                                                   >  2:1976408/1‑150 (MQ=255)

                                                                                 tCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTtgcct                                                                   <  1:104778/150‑1 (MQ=255)

                                                                                 tCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTtgcct                                                                   >  1:2280266/1‑150 (MQ=255)

                                                                                 ccGCCTGCCGGGTTGGTAAAGGTGACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACAGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTtgcct                                                                   <  1:1306024/149‑1 (MQ=255)

                                                                                   gCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCAcc                                                                                                                                                  <  2:247986/69‑1 (MQ=255)

                                                                                      tGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCGGACTGGTTGCCTGCCGc                                                              >  1:141952/1‑150 (MQ=255)

                                                                                      tGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGc                                                              >  1:2095213/1‑150 (MQ=255)

                                                                                          gggTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCg                                                          <  1:2056101/150‑1 (MQ=255)

                                                                                               ggTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAg                                                     <  2:2380793/150‑1 (MQ=255)

                                                                                                 tCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGca                                                   >  2:1390111/1‑150 (MQ=255)

                                                                                                   aCGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCa                                                      <  2:1769277/145‑1 (MQ=255)

                                                                                                   aCGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCa                                                      >  1:1769277/1‑145 (MQ=255)

                                                                                                     ggTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCTGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGcaccac                                               <  2:1846133/150‑1 (MQ=255)

                                                                                                      gTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACt                                              >  2:974123/1‑150 (MQ=255)

                                                                                                      gTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACt                                              <  1:2347203/150‑1 (MQ=255)

                                                                                                       tAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCa                                                                               >  1:1549384/1‑116 (MQ=255)

                                                                                                       tAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCa                                                                               <  2:1549384/116‑1 (MQ=255)

                                                                                                       tAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGc                                                              >  2:480698/1‑133 (MQ=255)

                                                                                                       tAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGc                                                              <  1:480698/133‑1 (MQ=255)

                                                                                                          cccGTGCGCCCCGTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCa                                          <  1:958301/150‑1 (MQ=255)

                                                                                                           ccGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCAc                                         >  2:1089066/1‑150 (MQ=255)

                                                                                                           ccGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCAc                                         <  2:906288/150‑1 (MQ=255)

                                                                                                             gTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCt                                       <  1:2416904/150‑1 (MQ=255)

                                                                                                                     aCTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGtt                                                                       <  1:945089/110‑1 (MQ=255)

                                                                                                                     aCTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGtt                                                                       >  2:945089/1‑110 (MQ=255)

                                                                                                                     aCTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTCGCAACGGGGGCGGTCATAGCCCTGGCGGCAGCCGCGTCGCCTGCCGCATCGGCCAGCACCACTTGCCCCGgttgcccc                               >  2:193548/1‑149 (MQ=255)

                                                                                                                            cgcgGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTGTTGGCCGTTGGCCt                        >  1:1400672/1‑150 (MQ=255)

                                                                                                                              cgGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTGTTGGCCGTTGGCCTGa                      <  1:275083/150‑1 (MQ=255)

                                                                                                                                   ggTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTGTTGGCCGTTGGCCTGAGGCGa                 >  2:1969761/1‑150 (MQ=255)

                                                                                                                                    gTGATGGTGTCACCCTCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGa                                                                                             >  2:2070444/1‑73 (MQ=38)

                                                                                                                                    gTGAGGGTGACCCCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCAc                                         >  2:700309/1‑125 (MQ=255)

                                                                                                                                    gTGAGGGGGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCAc                                         <  1:700309/125‑1 (MQ=255)

                                                                                                                                       atggTGTCACCCTCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTGTTGGCCGTTGGCCTGAGGCGATACg             >  1:692381/3‑150 (MQ=255)

                                                                                                                                         ggTGTCACCCTCACCGGAAACGGCCAGTTCGCCgg                                                                                                                              >  1:2045694/1‑35 (MQ=255)

                                                                                                                                         ggTGTCACCCTCACCGGAAACGGCCAGTTCGCCgg                                                                                                                              <  2:2045694/35‑1 (MQ=255)

                                                                                                                                         ggTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTGTTGGCCGTTGGCCTGAGGCGATACGAg           >  2:173142/1‑150 (MQ=255)

                                                                                                                                          gTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTGTTGGCCGTTGGCCTGa                      <  1:1766627/138‑1 (MQ=255)

                                                                                                                                          gTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTGTTGGCCGTTGGCCTGa                      >  2:1766627/1‑138 (MQ=255)

                                                                                                                                               acCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTGTTGGCCGTTGGCCTGAGGCGATACGAGGTTGAc     <  2:2045089/150‑1 (MQ=255)

                                                                                                                                               acCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTGTTGGCCGTTGGCCTGAGGCGATACGAGGTTGAc     >  2:181421/1‑150 (MQ=255)

                                                                                                                                                 cATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTGTTGGCCGTTGGCCTGAGGCGATACGAGGTTGACCt   >  2:213503/1‑150 (MQ=255)

                                                                                                                                                  aTCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGATATGCACCACTTGCACCTGTGGGACGTTGGCCTGAGGCGATACGAGGTTGACCTg  >  2:1245101/1‑150 (MQ=255)

AGCAGCTCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTGTTGGCCGTTGGCCTGAGGCGATACGAGGTTGACCTG  >  NC_002947/931364‑931659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`