| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 955,158 | C→T | 12.0% | intergenic (+109/‑204) | cyoE → / → alaC | protoheme IX farnesyltransferase/aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 955,158 | 0 | C | T | 12.0% | 93.1 / 2.3 | 40 | intergenic (+109/‑204) | cyoE/alaC | protoheme IX farnesyltransferase/aminotransferase |
| Reads supporting (aligned to +/- strand): ref base C (19/16); new base T (2/3); total (21/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGATTCAG > NC_002947/955013‑955300 | gACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAACCTGCTTGTTGTTTCATTAAAACCCCGGCCACTGCGGGGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTa < 1:1344860/150‑1 (MQ=38)gACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTa < 2:1495048/150‑1 (MQ=255) aCCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAg < 1:718685/150‑1 (MQ=255) cTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGc < 2:1534015/150‑1 (MQ=255) gCGGATGTGCTGATGACCTACGCACGCTGAAATTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTT‑CTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCT‑TGTAGGAGCggg < 2:151784/150‑1 (MQ=25) cGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGACTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCT‑TGTAGGAGCggg < 1:1085192/150‑1 (MQ=37) tgCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTAcc < 2:622263/150‑1 (MQ=255) gatgaCCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCcgcg > 2:356075/1‑150 (MQ=255) atgaCCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCgg < 1:1361037/138‑1 (MQ=255) atgaCCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCgg > 2:1361037/1‑138 (MQ=255) ccTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAagag < 1:356075/150‑1 (MQ=255) acgcTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCC‑GGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCT‑TGTAGGAGCgg > 2:1896783/1‑126 (MQ=25) acgcTGAAACTGCCGCCTGTTTCACGAAAACCCCGGCCACTGTGCC‑GGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCT‑TGTAGGAGCgg < 1:1896783/126‑1 (MQ=25) ccccGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTAC‑AGGAACCTTGTAGGAGCgg > 1:2393081/1‑97 (MQ=18) ccGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCgg < 2:19124/95‑1 (MQ=255) ccGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCgg > 1:19124/1‑95 (MQ=255) cTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCgg > 1:963811/1‑88 (MQ=255) cTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCgg < 2:963811/88‑1 (MQ=255) gggTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑t < 2:160482/70‑1 (MQ=255) gggTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑t > 1:160482/1‑70 (MQ=255) gggTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGACATCCTGTTTCgg > 1:991982/1‑150 (MQ=255) aaaCCCGCTCCTAC‑AGGAACCTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCta > 1:2327505/1‑106 (MQ=17) aaaCCCGCTCCTAC‑AGGAACCTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCta < 2:2327505/106‑1 (MQ=17) cccGCTCCTACAGGAACCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAg > 2:810500/1‑129 (MQ=25) cccGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACa < 2:1697424/128‑1 (MQ=255) cccGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACa > 1:1697424/1‑128 (MQ=255) cccGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtc > 2:2147787/1‑150 (MQ=255) cccGCTCCTAC‑AGGAACCTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATaaa < 1:1973366/108‑1 (MQ=21) cccGCTCCTAC‑AGGAACCTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATaaa < 1:1973365/108‑1 (MQ=21) cccGCTCCTAC‑AGGAACCTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATaaa > 2:1973366/1‑108 (MQ=21) cccGCTCCTAC‑AGGAACCTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATaaa > 2:1973365/1‑108 (MQ=21) cccGCTCCTAC‑AGGAACCTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAg < 1:810500/129‑1 (MQ=25) ccGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtct > 1:1746897/1‑150 (MQ=255) ccGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtct > 2:1053177/1‑150 (MQ=255) ccGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtct > 1:138086/1‑150 (MQ=255) gCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTc < 2:1039197/138‑1 (MQ=255) gCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTc > 1:1039197/1‑138 (MQ=255) cTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtcttct > 2:997271/1‑150 (MQ=255) tACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGAtt > 1:1067504/1‑150 (MQ=255) aaGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACATAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGATTCAg > 2:75741/1‑150 (MQ=255) c‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGa > 2:203982/1‑71 (MQ=255) c‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGa < 1:203982/71‑1 (MQ=255) | GACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGATTCAG > NC_002947/955013‑955300 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |