Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 146,128 T→A 8.1% intergenic (+33/‑115) gltP → / → PP_0138 glutamate/aspartate‑proton DAACS transporter/membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947146,1280TA 8.1% 333.7 / 17.1 111intergenic (+33/‑115)gltP/PP_0138glutamate/aspartate‑proton DAACS transporter/membrane protein
Reads supporting (aligned to +/- strand):  ref base T (50/52);  new base A (5/4);  total (55/56)
Fisher's exact test for biased strand distribution p-value = 7.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01

Only 100 of 111 total aligned reads displayed.

GTCATTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGATGCTCAACGGCCTTTGGCTCGGCTTTTTCCTG  >  NC_002947/145982‑146275
                                                                                                                                                  |                                                                                                                                                   
gTCATTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTc                                                                                                                                                  <  2:2318671/150‑1 (MQ=255)
gTCATTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTc                                                                                                                                                  >  1:998554/1‑150 (MQ=255)
 tCATTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCg                                                                                                                                                 >  1:1190675/1‑150 (MQ=255)
  cATTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCgg                                                                                                                                                <  2:846819/150‑1 (MQ=255)
  cATTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCgg                                                                                                                                                <  1:799530/150‑1 (MQ=255)
   aTTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGCGTCTTATTGCTGTTTGACCAAGAGCCCCGACTTGTCggg                                                                                                                                               >  2:2018395/1‑150 (MQ=255)
        tCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCttt                                                                                                                                          >  2:869376/1‑150 (MQ=255)
        tCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCttt                                                                                                                                          <  1:233824/150‑1 (MQ=255)
          gCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCttttt                                                                                                                                        >  1:29617/1‑150 (MQ=255)
          gCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACAAGTCggg                                                                                                                                               >  1:1160577/1‑143 (MQ=255)
          gCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACAAGTCggg                                                                                                                                               <  2:1160577/143‑1 (MQ=255)
           cTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCGAAGCGCTGCGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTg                                                                                                                                       <  1:1232776/150‑1 (MQ=255)
           cTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCAAGCTGATGGCCGGGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCGAGAGGGCTGCCTGTTGTCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTg                                                                                                                                       <  1:1225354/150‑1 (MQ=255)
               gAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTAt                                                                                                                                   >  2:366966/1‑150 (MQ=255)
                   gCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGc                                                                                                                               >  1:702337/1‑150 (MQ=255)
                    cATGTACGACGCGGTCCAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCg                                                                                                                              >  1:1775369/1‑150 (MQ=255)
                    cATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACAAGTCgg                                                                                                                                                >  2:2050071/1‑132 (MQ=255)
                     aTGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCga                                                                                                                             <  1:1720414/150‑1 (MQ=255)
                        tACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGAt                                                                                                                          >  1:1960419/1‑150 (MQ=255)
                        tACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGAt                                                                                                                          >  1:489438/1‑150 (MQ=255)
                             cgcgGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGa                                                                                                                     <  1:926369/150‑1 (MQ=255)
                              gcgGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTg                                                                                                                      <  1:443856/148‑1 (MQ=255)
                              gcgGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTg                                                                                                                      >  2:443856/1‑148 (MQ=255)
                                    aaGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCgcgc                                                                                                              >  1:2094852/1‑150 (MQ=255)
                                            gCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCga                                                                                                                             <  1:2084955/127‑1 (MQ=255)
                                            gCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCga                                                                                                                             >  2:2084955/1‑127 (MQ=255)
                                            gCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAg                                                                                                      >  1:278834/1‑150 (MQ=255)
                                            gCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACCTGTCGGGGCTTTTTGTTATCTGCGAGTTCTTGTTCCGCCCCTAGaacg                                                                                                      <  2:467441/150‑3 (MQ=255)
                                                tactacGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTc                                                                                                  <  1:1627662/150‑1 (MQ=255)
                                                tactacGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTc                                                                                                  <  1:475249/150‑1 (MQ=255)
                                                tactacGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACAAGTCGGGGCtcttg                                                                                                                                        >  2:875108/1‑108 (MQ=255)
                                                tactacGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACAAGTCGGGGCtcttg                                                                                                                                        <  1:875108/112‑2 (MQ=255)
                                                  ctacGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCgg                                                                                                                                                <  2:2279399/102‑1 (MQ=255)
                                                  ctacGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCgg                                                                                                                                                >  1:2279399/1‑102 (MQ=255)
                                                    acGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGa                                                                                              <  1:692410/150‑1 (MQ=255)
                                                      gCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATc                                                                                            <  2:29617/150‑1 (MQ=255)
                                                       ccTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCg                                                                                           <  2:1823845/150‑1 (MQ=255)
                                                        cTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGc                                                                                          <  2:278834/150‑1 (MQ=255)
                                                          cGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCCAGCGCTGAGCCTGAGGGCTGTTTGGCCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCa                                                                                        >  2:453038/1‑150 (MQ=255)
                                                           gCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGc                                                                                                                               >  1:32014/1‑110 (MQ=255)
                                                           gCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGc                                                                                                                               <  2:32014/110‑1 (MQ=255)
                                                            cTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGCGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGt                                                                                      <  2:1190675/150‑1 (MQ=255)
                                                             tGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTc                                                                                     >  2:2181930/1‑150 (MQ=255)
                                                             tGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTc                                                                                     <  1:366966/150‑1 (MQ=255)
                                                                  gCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAgg                                                                                <  2:1960419/150‑1 (MQ=255)
                                                                  gCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAgg                                                                                <  1:2392960/150‑1 (MQ=255)
                                                                  gCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAgg                                                                                >  1:2315663/1‑150 (MQ=255)
                                                                    cGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCt                                                                              >  1:1735317/1‑150 (MQ=255)
                                                                        aaGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGTCTGCGa                                                                          <  2:747695/150‑1 (MQ=255)
                                                                          gCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTTCAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATa                                                                        >  1:48092/1‑150 (MQ=255)
                                                                          gCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATa                                                                        <  1:1260270/150‑1 (MQ=255)
                                                                            aGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACg                                                                      <  2:1614113/150‑1 (MQ=255)
                                                                              ggAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCTCGATCGCCAGTCACAGGCTGCGATACGCt                                                                    >  2:1947274/1‑150 (MQ=255)
                                                                                 gCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCtatt                                                                 >  2:1493835/1‑150 (MQ=255)
                                                                                  cTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCtatta                                                                >  2:2328199/1‑150 (MQ=255)
                                                                                    gTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCga                                                                                                                             <  1:484887/87‑1 (MQ=255)
                                                                                    gTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCga                                                                                                                             >  2:484887/1‑87 (MQ=255)
                                                                                     tAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAg                                                                                                      >  1:2197869/1‑109 (MQ=255)
                                                                                      aGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCg                                                            <  2:2193082/150‑1 (MQ=255)
                                                                                       gTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCgg                                                           <  2:2312906/150‑1 (MQ=255)
                                                                                       gTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCgg                                                           <  1:1424124/150‑1 (MQ=255)
                                                                                       gTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCgg                                                           <  1:1253696/150‑1 (MQ=255)
                                                                                        tGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCggg                                                          <  2:702337/150‑1 (MQ=255)
                                                                                              gCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTcg                                                                                                 >  2:598560/1‑105 (MQ=255)
                                                                                              gCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTcg                                                                                                 <  1:598560/105‑1 (MQ=255)
                                                                                               cGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACtttt                                                   <  2:1976422/150‑1 (MQ=255)
                                                                                                      gCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGa                                                                          >  1:1907973/1‑120 (MQ=255)
                                                                                                      gCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCggg                                                          <  1:2430024/136‑1 (MQ=255)
                                                                                                      gCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCggg                                                          >  2:2430024/1‑136 (MQ=255)
                                                                                                      gCCAAGAGCTGAGCCTGATGGCTGTTTGACCAAGAGGAGCGAGTTGTCGGGGGTTTTTGTGATCTGGGAGATCTTGGGAGGCGCCTACAAAGGATAGCGATAGCCAGTCACAGGATGCGATAAGCTATTATTAGGGCACTTTTAAggggg                                            >  2:1010265/1‑150 (MQ=255)
                                                                                                       ccAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAg                                                >  1:1685038/1‑145 (MQ=255)
                                                                                                       ccAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAg                                                <  2:1685038/145‑1 (MQ=255)
                                                                                                       ccAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGa                                           >  2:692794/1‑150 (MQ=255)
                                                                                                          aGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACa                                        <  2:489438/150‑1 (MQ=255)
                                                                                                           gcgcTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGGTCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACCGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACAt                                       >  1:1813912/1‑150 (MQ=255)
                                                                                                           gcgcTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCGAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACAt                                       >  2:1252961/1‑150 (MQ=255)
                                                                                                             gcTGAGCCTGATGGCTGTTTCACCAAGATCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCtt                                                                                                                       >  2:756616/1‑68 (MQ=255)
                                                                                                               tGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACgg                                   >  1:175580/1‑150 (MQ=255)
                                                                                                                gAGCCTGATGGCTTTTTGACCAAGAGCCCCGACTTCTAGGGCCTGTTTGTTATCTGCGAGATCTTGAGCCGTGAACACAAAGGCTCGCGATCGCCAGTCACAGTCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGa                                  <  1:757893/150‑1 (MQ=255)
                                                                                                                gAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTTCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGa                                  <  1:1855855/150‑1 (MQ=255)
                                                                                                                   ccTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGATGc                               <  1:2061188/150‑1 (MQ=255)
                                                                                                                    cTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGATGCt                              >  2:2197612/1‑150 (MQ=255)
                                                                                                                    cTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGATGCt                              <  2:1735317/150‑1 (MQ=255)
                                                                                                                           cTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGATGCTCAACGGc                       <  1:929345/150‑1 (MQ=255)
                                                                                                                            tGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGATGCTCAACGGcc                      <  1:869376/150‑1 (MQ=255)
                                                                                                                                tGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGATGCTCAACGGCCTTTg                  >  1:2181874/1‑150 (MQ=255)
                                                                                                                                 gACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGATGCTCAACGGCCTTTgg                 <  1:453038/150‑1 (MQ=255)
                                                                                                                                    cAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATa                                      <  1:327317/126‑1 (MQ=255)
                                                                                                                                    cAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATa                                      >  2:327317/1‑126 (MQ=255)
                                                                                                                                    cAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGATGCTCAACGGCCTTTGGCTc              >  2:2440564/1‑150 (MQ=255)
                                                                                                                                       gagCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGATGCTCAACGGCCTTTGGCTCGGc           >  2:2296407/1‑150 (MQ=255)
                                                                                                                                        agCCCCGACAAGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCgg                                                           <  1:1627378/101‑1 (MQ=255)
                                                                                                                                        agCCCCGACAAGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCgg                                                           >  2:1627378/1‑101 (MQ=255)
                                                                                                                                          ccccGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGATGCTCAACGGCCTTTgg                 >  1:840284/1‑141 (MQ=255)
                                                                                                                                          ccccGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGATGCTCAACGGCCTTTgg                 <  2:840284/141‑1 (MQ=255)
                                                                                                                                          ccccGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCCATCGCCAGTCACAGGCTGCGATACGCTATGATTCGGGCACTTTTCAGGGGGCACATACGGCTGCTCAACGGCCTTTGGCTCGGCttt        >  2:644326/1‑150 (MQ=255)
                                                                                                                                          ccccGACAAGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGACTTTTAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGATGCTCAACGGCCTTTGGCTCGGCttt        >  1:1240746/1‑150 (MQ=255)
                                                                                                                                            ccGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGATGCTCAACGGCCTTTGGCTCGGCttttt      >  1:1351856/1‑150 (MQ=255)
                                                                                                                                             cGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGATGCTCAACGGCCTTTGGCTCGGCTTTTTc     >  1:1193132/1‑150 (MQ=255)
                                                                                                                                                cTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGATGCTCAACGGCCTTTGGCTCGGCTTTTTCCtg  <  1:2181930/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GTCATTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGATGCTCAACGGCCTTTGGCTCGGCTTTTTCCTG  >  NC_002947/145982‑146275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑